Restriction map for
pFN21AB5562
(Circular) (Six-base) MAPSORT of: pFN21AB5562.seq Check: 6700 from: 1 to: 7066
pFN21AB5562 7066 bp
With 182 enzymes: SgfI *
October 20, 2009 10:33 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2948 3940 2948
Size: 992 6074
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4876 278
Size: 53 83 186 936 3340 2468
Fragments arranged by size:
3340 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 4409 1937
Size: 2472 4594
Acc65I G'GTAC_C
Cuts at: 2350 3541 4387 2350
Size: 1191 846 5029
Fragments arranged by size:
5029 1191 846
AclI AA'CG_TT
Cuts at: 1563 5193 5566 1563
Size: 3630 373 3063
Fragments arranged by size:
3630 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2670 3231 4009 5129 6513 6781 1632
Size: 1038 561 778 1120 1384 268 1917
Fragments arranged by size:
1917 1384 1120 1038 778 561 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6869
AflIII A'CryG_T
Cuts at: 1203 4228 5965 6942 1203
Size: 3025 1737 977 1327
Fragments arranged by size:
3025 1737 1327 977
AgeI A'CCGG_T
Cuts at: 5897 5897
Size: 7066
AhdI GACnn_n'nnGTC
Cuts at: 5795 5795
Size: 7066
AleI CACnn'nnGTG
Cuts at: 1166 3128 3141 3392 1166
Size: 1962 13 251 4840
Fragments arranged by size:
4840 1962 251 13
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2268 1184
Size: 1084 5982
AlwNI CAG_nnn'CTG
Cuts at: 2494 2837 2990 3848 4809 6381 2494
Size: 343 153 858 961 1572 3179
Fragments arranged by size:
3179 1572 961 858 343 153
ApaLI G'TGCA_C
Cuts at: 2642 5123 6279 2642
Size: 2481 1156 3429
Fragments arranged by size:
3429 2481 1156
ApoI r'AATT_y
Cuts at: 1493 2091 2177 3197 3799 4328 4375 4601 4753
Size: 598 86 1020 602 529 47 226 152
Cuts at: 4753 6862 1493
Size: 2109 1697
Fragments arranged by size:
2109 1697 1020 602 598 529 226 152
86 47
AseI AT'TA_AT
Cuts at: 160 1032 5620 160
Size: 872 4588 1606
Fragments arranged by size:
4588 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7066
AvaI C'yCGr_G
Cuts at: 1870 1943 4391 1870
Size: 73 2448 4545
Fragments arranged by size:
4545 2448 73
BamHI G'GATC_C
Cuts at: 4396 4763 4396
Size: 367 6699
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2350 3502 3541 4046 4387
Size: 325 217 627 563 1152 39 505 341
Cuts at: 4387 5842 618
Size: 1455 1842
Fragments arranged by size:
1842 1455 1152 627 563 505 341 325
217 39
BanII G_rGCy'C
Cuts at: 1391 2252 2780 3491 4385 1391
Size: 861 528 711 894 4072
Fragments arranged by size:
4072 894 861 711 528
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2748 3219 4264 928
Size: 971 849 471 1045 3730
Fragments arranged by size:
3730 1045 971 849 471
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 5257 5291 1903
Size: 34 3320 34 3678
Fragments arranged by size:
3678 3320 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2720 4958 6168 2720
Size: 2238 1210 3618
Fragments arranged by size:
3618 2238 1210
BclI T'GATC_A
Cuts at: 1548 2197 2485 2635 1548
Size: 649 288 150 5979
Fragments arranged by size:
5979 649 288 150
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2009 4183 5677
Size: 107 122 71 631 605 337 2174 1494
Cuts at: 5677 136
Size: 1525
Fragments arranged by size:
2174 1525 1494 631 605 337 122 107
71
BglII A'GATC_T
Cuts at: 7061 7061
Size: 7066
BlpI GC'TnA_GC
Cuts at: 2849 4483 2849
Size: 1634 5432
Bme1580I G_kGCm'C
Cuts at: 1790 2368 2646 4051 5127 6283 1790
Size: 578 278 1405 1076 1156 2573
Fragments arranged by size:
2573 1405 1156 1076 578 278
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 7066
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2063 2825 3982
Size: 416 53 83 399 24 646 762 1157
Cuts at: 3982 4797 5755 442
Size: 815 958 1753
Fragments arranged by size:
1753 1157 958 815 762 646 416 399
83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 7066
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2266 3112 3505 2266
Size: 846 393 5827
Fragments arranged by size:
5827 846 393
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 3500 3709 5726 1239
Size: 2261 209 2017 2579
Fragments arranged by size:
2579 2261 2017 209
Bpu10I CC'TnA_GC
Cuts at: 3490 3931 3490
Size: 441 6625
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2764 3650 5191 6056 6354 2764
Size: 886 1541 865 298 3476
Fragments arranged by size:
3476 1541 886 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 5729 882
Size: 617 4230 2219
Fragments arranged by size:
4230 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 4857 493
Size: 711 3653 2702
Fragments arranged by size:
3653 2702 711
BsaBI GATnn'nnATC
Cuts at: 2196 3663 7060 2196
Size: 1467 3397 2202
Fragments arranged by size:
3397 2202 1467
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4873 5255 5890 275
Size: 53 83 186 936 3340 382 635 1451
Fragments arranged by size:
3340 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 3285 3291 5499 5897 6171 6318 6729 3285
Size: 6 2208 398 274 147 411 3622
Fragments arranged by size:
3622 2208 411 398 274 147 6
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2097 2127 3031 3061 3760
Size: 30 165 30 694 30 904 30 699
Cuts at: 3760 3790 4271 4301 1178
Size: 30 481 30 3943
Fragments arranged by size:
3943 904 699 694 481 165 30 30
30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2118 3712 1186
Size: 932 1594 4540
Fragments arranged by size:
4540 1594 932
BseYI C'CCAG_C
Cuts at: 2953 3945 4294 6269 6765 2953
Size: 992 349 1975 496 3254
Fragments arranged by size:
3254 1975 992 496 349
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2911 1163
Size: 573 1175 5318
Fragments arranged by size:
5318 1175 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3791 4516 5277 6305 1540
Size: 275 186 1790 725 761 1028 2301
Fragments arranged by size:
2301 1790 1028 761 725 275 186
BsiHKAI G_wGCw'C
Cuts at: 2252 2646 5127 5212 6283 2252
Size: 394 2481 85 1071 3035
Fragments arranged by size:
3035 2481 1071 394 85
BsmI GAATG_Cn'
Cuts at: 4577 4670 4577
Size: 93 6973
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2252 2368 2646 2780 3491 4051 4385
Size: 399 462 116 278 134 711 560 334
Cuts at: 4385 5127 5212 6283 1391
Size: 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 711 560 462 399 334
278 134 116 85
BspEI T'CCGG_A
Cuts at: 6729 6729
Size: 7066
BspHI T'CATG_A
Cuts at: 4955 6685 4955
Size: 1730 5336
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2948 3025 3940 4421 844
Size: 2104 77 915 481 3489
Fragments arranged by size:
3489 2104 915 481 77
BsrBI CCG'CTC
Cuts at: 1394 1823 2221 3930 4953 1394
Size: 429 398 1709 1023 3507
Fragments arranged by size:
3507 1709 1023 429 398
BsrDI GCAATG_nn'
Cuts at: 65 1248 2064 5561 5735 65
Size: 1183 816 3497 174 1396
Fragments arranged by size:
3497 1396 1183 816 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 3790 5710 5897 1700
Size: 110 1980 1920 187 2869
Fragments arranged by size:
2869 1980 1920 187 110
BsrGI T'GTAC_A
Cuts at: 96 3056 96
Size: 2960 4106
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 7066
BssSI C'ACGA_G
Cuts at: 5126 6138 5126
Size: 1012 6054
BstAPI GCAn_nnn'nTGC
Cuts at: 794 3009 794
Size: 2215 4851
BstBI TT'CG_AA
Cuts at: 5870 5870
Size: 7066
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 7066
BstYI r'GATC_y
Cuts at: 1972 2716 3664 4100 4396 4763 5148 5165 6617
Size: 744 948 436 296 367 385 17 1452
Cuts at: 6617 6703 6715 7061 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1452 948 744 436 385 367 346
296 86 17 12
Bsu36I CC'TnA_GG
Cuts at: 4266 4266
Size: 7066
BtgI C'CryG_G
Cuts at: 513 1065 2004 3416 513
Size: 552 939 1412 4163
Fragments arranged by size:
4163 1412 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 4044 487
Size: 683 56 2818 3509
Fragments arranged by size:
3509 2818 683 56
BtsI GCAGTG_nn'
Cuts at: 2923 4582 5376 5396 2923
Size: 1659 794 20 4593
Fragments arranged by size:
4593 1659 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 7066
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 7031
DraI TTT'AAA
Cuts at: 4370 4370
Size: 7066
DraIII CAC_nnn'GTG
Cuts at: 2914 2914
Size: 7066
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 6073 809
Size: 507 4757 1802
Fragments arranged by size:
4757 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2992 3018 3561
Size: 54 1425 50 275 18 1162 26 543
Cuts at: 3561 3788 4175 4258 4513 5402 7031 8
Size: 227 387 83 255 889 1629 43
Fragments arranged by size:
1629 1425 1162 889 543 387 275 255
227 83 54 50 43 26 18
EagI C'GGCC_G
Cuts at: 1537 1812 3788 4513 1537
Size: 275 1976 725 4090
Fragments arranged by size:
4090 1976 725 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3544 4996 1353
Size: 2191 1452 3423
Fragments arranged by size:
3423 2191 1452
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5653 6027 6173 387
Size: 1064 138 4064 374 146 1280
Fragments arranged by size:
4064 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2250 2250
Size: 7066
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2670 3231 3500 3709 4009 5129 5726
Size: 393 1038 561 269 209 300 1120 597
Cuts at: 5726 6513 6781 1239
Size: 787 268 1524
Fragments arranged by size:
1524 1120 1038 787 597 561 393 300
269 268 209
EcoO109I rG'GnC_Cy
Cuts at: 3458 3458
Size: 7066
EcoRI G'AATT_C
Cuts at: 2091 3197 4328 4375 4753 2091
Size: 1106 1131 47 378 4404
Fragments arranged by size:
4404 1131 1106 378 47
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2285 2285
Size: 7066
FspI TGC'GCA
Cuts at: 5572 5572
Size: 7066
HaeII r_GCGC'y
Cuts at: 1678 6213 1678
Size: 4535 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2033 2065 2097 2129 2189
Size: 32 135 32 461 32 32 32 60
Cuts at: 2189 2221 4271 4303 5707 5739 5781 5813 1373
Size: 32 2050 32 1404 32 42 32 2626
Fragments arranged by size:
2626 2050 1404 461 135 60 42 32
32 32 32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3080 4410 4656 669
Size: 988 281 1142 1330 246 3079
Fragments arranged by size:
3079 1330 1142 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 7066
HpaI GTT'AAC
Cuts at: 4656 4656
Size: 7066
KpnI G_GTAC'C
Cuts at: 2354 3545 4391 2354
Size: 1191 846 5029
Fragments arranged by size:
5029 1191 846
MfeI C'AATT_G
Cuts at: 4665 4665
Size: 7066
MluI A'CGCG_T
Cuts at: 6942 6942
Size: 7066
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2214 3265 3456 3909 6180 6364 1305
Size: 909 1051 191 453 2271 184 2007
Fragments arranged by size:
2271 2007 1051 909 453 191 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2994 3020 3563 4177 4260
Size: 54 1425 343 1162 26 543 614 83
Cuts at: 4260 10
Size: 2816
Fragments arranged by size:
2816 1425 1162 614 543 343 83 54
26
MslI CAynn'nnrTG
Cuts at: 518 1166 2649 2765 3128 3141 3392 5024 5383
Size: 648 1483 116 363 13 251 1632 359
Cuts at: 5383 5542 518
Size: 159 2042
Fragments arranged by size:
2042 1632 1483 648 363 359 251 159
116 13
MspA1I CmG'CkG
Cuts at: 1606 3141 3499 4482 5159 6307 6552 1606
Size: 1535 358 983 677 1148 245 2120
Fragments arranged by size:
2120 1535 1148 983 677 358 245
NaeI GCC'GGC
Cuts at: 3792 3792
Size: 7066
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 5575
Fragments arranged by size:
5575 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 7066
NgoMIV G'CCGG_C
Cuts at: 3790 3790
Size: 7066
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 7066
NotI GC'GGCC_GC
Cuts at: 4513 4513
Size: 7066
NspI r_CATG'y
Cuts at: 1132 1229 4232 4424 5969 1132
Size: 97 3003 192 1545 2229
Fragments arranged by size:
3003 2229 1545 192 97
PasI CC'CwG_GG
Cuts at: 1348 3760 1348
Size: 2412 4654
PciI A'CATG_T
Cuts at: 4228 5965 4228
Size: 1737 5329
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2990 3952 1108
Size: 1882 962 4222
Fragments arranged by size:
4222 1882 962
PfoI T'CCnGG_A
Cuts at: 2712 4275 2712
Size: 1563 5503
PmeI GTTT'AAAC
Cuts at: 4370 4370
Size: 7066
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3338 3370 5135 5167 6648 6680 3338
Size: 32 1765 32 1481 32 3724
Fragments arranged by size:
3724 1765 1481 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 3458 3458
Size: 7066
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 7066
PsiI TTA'TAA
Cuts at: 4636 4636
Size: 7066
PstI C_TGCA'G
Cuts at: 830 3853 4135 4418 830
Size: 3023 282 283 3478
Fragments arranged by size:
3478 3023 283 282
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 7066
PvuII CAG'CTG
Cuts at: 3499 3499
Size: 7066
SacI G_AGCT'C
Cuts at: 2252 2252
Size: 7066
SalI G'TCGA_C
Cuts at: 1936 4408 1936
Size: 2472 4594
SbfI CC_TGCA'GG
Cuts at: 4418 4418
Size: 7066
ScaI AGT'ACT
Cuts at: 5314 7026 5314
Size: 1712 5354
SfcI C'TryA_G
Cuts at: 826 1045 2359 3849 4131 4414 5549 6230 6421
Size: 219 1314 1490 282 283 1135 681 191
Cuts at: 6421 826
Size: 1471
Fragments arranged by size:
1490 1471 1314 1135 681 283 282 219
191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7066
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 7066
SmaI CCC'GGG
Cuts at: 1872 4393 1872
Size: 2521 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2779 3629 5170 6071 6333 820
Size: 197 926 836 850 1541 901 262 1553
Fragments arranged by size:
1553 1541 926 901 850 836 262 197
SnaBI TAC'GTA
Cuts at: 493 4857 493
Size: 4364 2702
SpeI A'CTAG_T
Cuts at: 152 3041 152
Size: 2889 4177
SphI G_CATG'C
Cuts at: 1132 4424 1132
Size: 3292 3774
SspI AAT'ATT
Cuts at: 5 52 3190 4990 5
Size: 47 3138 1800 2081
Fragments arranged by size:
3138 2081 1800 47
StuI AGG'CCT
Cuts at: 1517 2945 1517
Size: 1428 5638
StyI C'CwwG_G
Cuts at: 513 1065 2004 3895 4505 513
Size: 552 939 1891 610 3074
Fragments arranged by size:
3074 1891 939 610 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2661 3520 3758 3908 4157 5093 5110
Size: 1255 1081 859 238 150 249 936 17
Cuts at: 5110 5263 5448 5893 325
Size: 153 185 445 1498
Fragments arranged by size:
1498 1255 1081 936 859 445 249 238
185 153 150 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3056 3369 5312
Size: 274 80 33 51 1443 1079 313 1943
Cuts at: 5312 7024 96
Size: 1712 138
Fragments arranged by size:
1943 1712 1443 1079 313 274 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2408 2440 3760 3792 7026 7058 2408
Size: 32 1320 32 3234 32 2416
Fragments arranged by size:
3234 2416 1320 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 7066
XbaI T'CTAG_A
Cuts at: 4402 4402
Size: 7066
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2401 2610 2401
Size: 209 6857
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 7066
XmaI C'CCGG_G
Cuts at: 1870 4391 1870
Size: 2521 4545
XmnI GAAnn'nnTTC
Cuts at: 5195 5195
Size: 7066
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4874 276
Size: 53 83 186 936 3340 2468
Fragments arranged by size:
3340 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI
BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI
FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI
MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI
NgoMIV NheI NotI NspI PasI PciI PflMI PfoI
PmeI PpiI PpuMI PshAI PsiI PstI PvuI PvuII
SacI SalI SbfI ScaI SfcI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI
BfrBI BsiWI BsmBI BstXI BstZ17I EcoNI EcoRV FseI
FspAI KasI NarI NruI NsiI PacI PmlI PspOMI
PspXI PsrI RsrII SacII SanDI SapI SexAI SfiI
SfoI SrfI SwaI