Restriction map for
pFN21AB5556
(Circular) (Six-base) MAPSORT of: pFN21AB5556.seq Check: 4910 from: 1 to: 5698
pFN21AB5556 5698 bp
With 182 enzymes: SgfI *
January 16, 2009 15:25 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2643 2643
Size: 5698
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3508 278
Size: 53 83 186 936 1972 2468
Fragments arranged by size:
2468 1972 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2511 3041 1937
Size: 574 530 4594
Fragments arranged by size:
4594 574 530
Acc65I G'GTAC_C
Cuts at: 3019 3019
Size: 5698
AclI AA'CG_TT
Cuts at: 1563 3825 4198 1563
Size: 2262 373 3063
Fragments arranged by size:
3063 2262 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2171 2464 2699 3761 5145 5413 1632
Size: 539 293 235 1062 1384 268 1917
Fragments arranged by size:
1917 1384 1062 539 293 268 235
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5501
AflIII A'CryG_T
Cuts at: 1203 4597 5574 1203
Size: 3394 977 1327
Fragments arranged by size:
3394 1327 977
AgeI A'CCGG_T
Cuts at: 4529 4529
Size: 5698
AhdI GACnn_n'nnGTC
Cuts at: 2746 4427 2746
Size: 1681 4017
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5698
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5698
AlwNI CAG_nnn'CTG
Cuts at: 2296 2677 3441 5013 2296
Size: 381 764 1572 2981
Fragments arranged by size:
2981 1572 764 381
ApaLI G'TGCA_C
Cuts at: 3755 4911 3755
Size: 1156 4542
ApoI r'AATT_y
Cuts at: 1493 3007 3233 3385 5494 1493
Size: 1514 226 152 2109 1697
Fragments arranged by size:
2109 1697 1514 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4252 160
Size: 872 3220 1606
Fragments arranged by size:
3220 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5698
AvaI C'yCGr_G
Cuts at: 1870 1943 2225 2702 3023 1870
Size: 73 282 477 321 4545
Fragments arranged by size:
4545 477 321 282 73
BamHI G'GATC_C
Cuts at: 3028 3395 3028
Size: 367 5331
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2563 2961 3019 4474 618
Size: 325 217 627 776 398 58 1455 1842
Fragments arranged by size:
1842 1455 776 627 398 325 217 58
BanII G_rGCy'C
Cuts at: 1391 3017 1391
Size: 1626 4072
BbeI G_GCGC'C
Cuts at: 2567 2567
Size: 5698
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4727
BbvCI CC'TCA_GC
Cuts at: 2522 2522
Size: 5698
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3889 3923 1903
Size: 34 1952 34 3678
Fragments arranged by size:
3678 1952 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3590 4800 3590
Size: 1210 4488
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5698
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2453 2588 4309
Size: 107 122 71 631 605 781 135 1721
Cuts at: 4309 136
Size: 1525
Fragments arranged by size:
1721 1525 781 631 605 135 122 107
71
BglII A'GATC_T
Cuts at: 5693 5693
Size: 5698
BlpI GC'TnA_GC
Cuts at: 2546 3115 2546
Size: 569 5129
Bme1580I G_kGCm'C
Cuts at: 1790 2265 2303 2703 3759 4915 1790
Size: 475 38 400 1056 1156 2573
Fragments arranged by size:
2573 1156 1056 475 400 38
BmgBI CAC'GTC
Cuts at: 1318 2200 2397 1318
Size: 882 197 4619
Fragments arranged by size:
4619 882 197
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2132 2284 3429
Size: 416 53 83 399 24 715 152 1145
Cuts at: 3429 4387 442
Size: 958 1753
Fragments arranged by size:
1753 1145 958 715 416 399 152 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5698
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2252 2846 2864 4358 1239
Size: 1013 594 18 1494 2579
Fragments arranged by size:
2579 1494 1013 594 18
Bpu10I CC'TnA_GC
Cuts at: 2522 2906 2522
Size: 384 5314
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2195 3823 4688 4986 2195
Size: 1628 865 298 2907
Fragments arranged by size:
2907 1628 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4361 882
Size: 617 2862 2219
Fragments arranged by size:
2862 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3489 493
Size: 711 2285 2702
Fragments arranged by size:
2702 2285 711
BsaBI GATnn'nnATC
Cuts at: 5692 5692
Size: 5698
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2219 2564 2888 3505
Size: 53 83 186 936 686 345 324 617
Cuts at: 3505 3887 4522 275
Size: 382 635 1451
Fragments arranged by size:
1451 936 686 635 617 382 345 324
186 83 53
BsaWI w'CCGG_w
Cuts at: 4131 4529 4803 4950 5361 4131
Size: 398 274 147 411 4468
Fragments arranged by size:
4468 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2873 2903 1178
Size: 30 165 30 1470 30 3973
Fragments arranged by size:
3973 1470 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2174 2699 1186
Size: 988 525 4185
Fragments arranged by size:
4185 988 525
BseYI C'CCAG_C
Cuts at: 2387 2607 2640 4901 5397 2387
Size: 220 33 2261 496 2688
Fragments arranged by size:
2688 2261 496 220 33
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2351 2477 2675 1163
Size: 573 615 126 198 4186
Fragments arranged by size:
4186 615 573 198 126
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2583 3148 3909 4937 1540
Size: 275 186 582 565 761 1028 2301
Fragments arranged by size:
2301 1028 761 582 565 275 186
BsiHKAI G_wGCw'C
Cuts at: 3759 3844 4915 3759
Size: 85 1071 4542
Fragments arranged by size:
4542 1071 85
BsmI GAATG_Cn'
Cuts at: 3209 3302 3209
Size: 93 5605
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2265 2303 2703 3017 3759 3844 4915
Size: 399 475 38 400 314 742 85 1071
Cuts at: 4915 1391
Size: 2174
Fragments arranged by size:
2174 1071 742 475 400 399 314 85
38
BspEI T'CCGG_A
Cuts at: 5361 5361
Size: 5698
BspHI T'CATG_A
Cuts at: 2940 3587 5317 2940
Size: 647 1730 3321
Fragments arranged by size:
3321 1730 647
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2643 3053 844
Size: 1799 410 3489
Fragments arranged by size:
3489 1799 410
BsrBI CCG'CTC
Cuts at: 1394 1823 2073 2758 3585 1394
Size: 429 250 685 827 3507
Fragments arranged by size:
3507 827 685 429 250
BsrDI GCAATG_nn'
Cuts at: 65 1248 4193 4367 65
Size: 1183 2945 174 1396
Fragments arranged by size:
2945 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2410 2506 2582 4342 4529 1700
Size: 110 600 96 76 1760 187 2869
Fragments arranged by size:
2869 1760 600 187 110 96 76
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5698
BssHII G'CGCG_C
Cuts at: 1925 2350 2541 1925
Size: 425 191 5082
Fragments arranged by size:
5082 425 191
BssSI C'ACGA_G
Cuts at: 3758 4770 3758
Size: 1012 4686
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2635 2986 794
Size: 1841 351 3506
Fragments arranged by size:
3506 1841 351
BstBI TT'CG_AA
Cuts at: 4502 4502
Size: 5698
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5698
BstXI CCAn_nnnn'nTGG
Cuts at: 2743 2743
Size: 5698
BstYI r'GATC_y
Cuts at: 1972 2100 3028 3395 3780 3797 5249 5335 5347
Size: 128 928 367 385 17 1452 86 12
Cuts at: 5347 5693 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 928 385 367 346 128 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2439 2663 2892 513
Size: 552 1374 224 229 3319
Fragments arranged by size:
3319 1374 552 229 224
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2779 487
Size: 683 56 1553 3406
Fragments arranged by size:
3406 1553 683 56
BtsI GCAGTG_nn'
Cuts at: 3214 4008 4028 3214
Size: 794 20 4884
Fragments arranged by size:
4884 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5698
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5663
DraI TTT'AAA
Cuts at: 3002 3002
Size: 5698
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4705 809
Size: 507 3389 1802
Fragments arranged by size:
3389 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2130 2358 2580
Size: 54 1425 50 275 18 300 228 222
Cuts at: 2580 2895 3145 4034 5663 8
Size: 315 250 889 1629 43
Fragments arranged by size:
1629 1425 889 315 300 275 250 228
222 54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2580 3145 1537
Size: 275 768 565 4090
Fragments arranged by size:
4090 768 565 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3628 1353
Size: 2275 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2783 4285 4659 4805 387
Size: 1064 138 1194 1502 374 146 1280
Fragments arranged by size:
1502 1280 1194 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2171 2252 2464 2699 2846 2864 3761
Size: 393 539 81 212 235 147 18 897
Cuts at: 3761 4358 5145 5413 1239
Size: 597 787 268 1524
Fragments arranged by size:
1524 897 787 597 539 393 268 235
212 147 81 18
EcoNI CCTnn'n_nnAGG
Cuts at: 2681 2681
Size: 5698
EcoO109I rG'GnC_Cy
Cuts at: 2674 2674
Size: 5698
EcoRI G'AATT_C
Cuts at: 3007 3385 3007
Size: 378 5320
FseI GG_CCGG'CC
Cuts at: 2586 2586
Size: 5698
FspI TGC'GCA
Cuts at: 4204 4204
Size: 5698
HaeII r_GCGC'y
Cuts at: 1678 2239 2487 2567 4845 1678
Size: 561 248 80 2278 2531
Fragments arranged by size:
2531 2278 561 248 80
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2732 2764 2873 2905 4339
Size: 32 135 32 1160 32 109 32 1434
Cuts at: 4339 4371 4413 4445 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1434 1160 135 109 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2416 2953 3042 3288 669
Size: 988 281 478 537 89 246 3079
Fragments arranged by size:
3079 988 537 478 281 246 89
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5698
HpaI GTT'AAC
Cuts at: 2953 3288 2953
Size: 335 5363
KasI G'GCGC_C
Cuts at: 2563 2563
Size: 5698
KpnI G_GTAC'C
Cuts at: 3023 3023
Size: 5698
MfeI C'AATT_G
Cuts at: 3297 3297
Size: 5698
MluI A'CGCG_T
Cuts at: 5574 5574
Size: 5698
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4812 4996 1305
Size: 3507 184 2007
Fragments arranged by size:
3507 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3876
Fragments arranged by size:
3876 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2741 3656 4015 4174 518
Size: 648 1575 915 359 159 2042
Fragments arranged by size:
2042 1575 915 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2441 2466 2472 2553 2665 3114 3791 4939
Size: 835 25 6 81 112 449 677 1148
Cuts at: 4939 5184 1606
Size: 245 2120
Fragments arranged by size:
2120 1148 835 677 449 245 112 81
25 6
NaeI GCC'GGC
Cuts at: 2584 2584
Size: 5698
NarI GG'CG_CC
Cuts at: 2564 2564
Size: 5698
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5146
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5698
NgoMIV G'CCGG_C
Cuts at: 2582 2582
Size: 5698
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5698
NotI GC'GGCC_GC
Cuts at: 3145 3145
Size: 5698
NspI r_CATG'y
Cuts at: 1132 1229 2905 3056 4601 1132
Size: 97 1676 151 1545 2229
Fragments arranged by size:
2229 1676 1545 151 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5698
PciI A'CATG_T
Cuts at: 4597 4597
Size: 5698
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5698
PmeI GTTT'AAAC
Cuts at: 3002 3002
Size: 5698
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3767 3799 5280 5312 3767
Size: 32 1481 32 4153
Fragments arranged by size:
4153 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5698
PsiI TTA'TAA
Cuts at: 3268 3268
Size: 5698
PstI C_TGCA'G
Cuts at: 830 2673 3050 830
Size: 1843 377 3478
Fragments arranged by size:
3478 1843 377
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5698
SacII CC_GC'GG
Cuts at: 2442 2666 2442
Size: 224 5474
SalI G'TCGA_C
Cuts at: 1936 3040 1936
Size: 1104 4594
SbfI CC_TGCA'GG
Cuts at: 3050 3050
Size: 5698
ScaI AGT'ACT
Cuts at: 3946 5658 3946
Size: 1712 3986
SfcI C'TryA_G
Cuts at: 826 1045 2086 2669 3046 4181 4862 5053 826
Size: 219 1041 583 377 1135 681 191 1471
Fragments arranged by size:
1471 1135 1041 681 583 377 219 191
SfiI GGCCn_nnn'nGGCC
Cuts at: 2588 2588
Size: 5698
SfoI GGC'GCC
Cuts at: 2565 2565
Size: 5698
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5698
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5698
SmaI CCC'GGG
Cuts at: 1872 3025 1872
Size: 1153 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2210 3802 4703 4965 820
Size: 197 926 267 1592 901 262 1553
Fragments arranged by size:
1592 1553 926 901 267 262 197
SnaBI TAC'GTA
Cuts at: 493 3489 493
Size: 2996 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5698
SphI G_CATG'C
Cuts at: 1132 3056 1132
Size: 1924 3774
SspI AAT'ATT
Cuts at: 5 52 3622 5
Size: 47 3570 2081
Fragments arranged by size:
3570 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5698
StyI C'CwwG_G
Cuts at: 513 1065 3137 513
Size: 552 2072 3074
Fragments arranged by size:
3074 2072 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2303 2949 3725 3742 3895 4080 4525
Size: 1255 723 646 776 17 153 185 445
Cuts at: 4525 325
Size: 1498
Fragments arranged by size:
1498 1255 776 723 646 445 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3944 5656 96
Size: 274 80 33 51 1443 1967 1712 138
Fragments arranged by size:
1967 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5658 5690 5658
Size: 32 5666
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5698
XbaI T'CTAG_A
Cuts at: 3034 3034
Size: 5698
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2954 2954
Size: 5698
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5698
XmaI C'CCGG_G
Cuts at: 1870 3023 1870
Size: 1153 4545
XmnI GAAnn'nnTTC
Cuts at: 3827 3827
Size: 5698
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3506 276
Size: 53 83 186 936 1972 2468
Fragments arranged by size:
2468 1972 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI
BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI
EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FseI
FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI
MfeI MluI MmeI MscI MslI MspA1I NaeI NarI
NcoI NdeI NgoMIV NheI NotI NspI PasI PciI
PflMI PmeI PpiI PshAI PsiI PstI PvuI SacII
SalI SbfI ScaI SfcI SfiI SfoI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AfeI AloI ApaI AscI AvrII BaeI BfrBI BplI
BsiWI BsmBI BstZ17I Bsu36I DraIII EcoICRI EcoRV FalI
FspAI NruI NsiI PacI PfoI PmlI PpuMI PspOMI
PspXI PsrI PvuII RsrII SacI SanDI SapI SexAI
SrfI SwaI