Restriction map for
pFN21AB5555
(Circular) (Six-base) MAPSORT of: pFN21AB5555.seq Check: 7110 from: 1 to: 6061
pFN21AB5555 6061 bp
With 182 enzymes: SgfI *
January 16, 2009 15:25 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3871 278
Size: 53 83 186 936 2335 2468
Fragments arranged by size:
2468 2335 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3404 1937
Size: 1467 4594
Acc65I G'GTAC_C
Cuts at: 3382 3382
Size: 6061
AclI AA'CG_TT
Cuts at: 1563 4188 4561 1563
Size: 2625 373 3063
Fragments arranged by size:
3063 2625 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 4124 5508 5776 1632
Size: 2492 1384 268 1917
Fragments arranged by size:
2492 1917 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5864
AflIII A'CryG_T
Cuts at: 1203 4960 5937 1203
Size: 3757 977 1327
Fragments arranged by size:
3757 1327 977
AgeI A'CCGG_T
Cuts at: 4892 4892
Size: 6061
AhdI GACnn_n'nnGTC
Cuts at: 2050 2814 4790 2050
Size: 764 1976 3321
Fragments arranged by size:
3321 1976 764
AleI CACnn'nnGTG
Cuts at: 1166 2558 3332 1166
Size: 1392 774 3895
Fragments arranged by size:
3895 1392 774
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6061
AlwNI CAG_nnn'CTG
Cuts at: 2800 2964 3109 3804 5376 2800
Size: 164 145 695 1572 3485
Fragments arranged by size:
3485 1572 695 164 145
ApaI G_GGCC'C
Cuts at: 2807 3019 2807
Size: 212 5849
ApaLI G'TGCA_C
Cuts at: 3056 4118 5274 3056
Size: 1062 1156 3843
Fragments arranged by size:
3843 1156 1062
ApoI r'AATT_y
Cuts at: 1493 2707 3080 3370 3596 3748 5857 1493
Size: 1214 373 290 226 152 2109 1697
Fragments arranged by size:
2109 1697 1214 373 290 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4615 160
Size: 872 3583 1606
Fragments arranged by size:
3583 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6061
AvaI C'yCGr_G
Cuts at: 1870 1943 2113 3386 1870
Size: 73 170 1273 4545
Fragments arranged by size:
4545 1273 170 73
BamHI G'GATC_C
Cuts at: 2228 3391 3758 2228
Size: 1163 367 4531
Fragments arranged by size:
4531 1163 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3382 4837 618
Size: 325 217 627 1595 1455 1842
Fragments arranged by size:
1842 1595 1455 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2807 3019 3380 1391
Size: 1416 212 361 4072
Fragments arranged by size:
4072 1416 361 212
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2664 928
Size: 971 765 4325
Fragments arranged by size:
4325 971 765
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2090 2124 4252 4286 1903
Size: 34 153 34 2128 34 3678
Fragments arranged by size:
3678 2128 153 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3953 5163 3953
Size: 1210 4851
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6061
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4672 136
Size: 107 122 71 631 605 3000 1525
Fragments arranged by size:
3000 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6056 6056
Size: 6061
BlpI GC'TnA_GC
Cuts at: 3008 3478 3008
Size: 470 5591
Bme1580I G_kGCm'C
Cuts at: 1790 2746 2807 3019 3060 4122 5278 1790
Size: 956 61 212 41 1062 1156 2573
Fragments arranged by size:
2573 1156 1062 956 212 61 41
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6061
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3792 4750 442
Size: 416 53 83 399 24 2375 958 1753
Fragments arranged by size:
2375 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6061
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2893 2893
Size: 6061
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2450 2888 3226 4721 1239
Size: 1211 438 338 1495 2579
Fragments arranged by size:
2579 1495 1211 438 338
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4186 5051 5349 4186
Size: 865 298 4898
Fragments arranged by size:
4898 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4724 882
Size: 617 3225 2219
Fragments arranged by size:
3225 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3334 3852 493
Size: 711 2130 518 2702
Fragments arranged by size:
2702 2130 711 518
BsaBI GATnn'nnATC
Cuts at: 2462 2790 6055 2462
Size: 328 3265 2468
Fragments arranged by size:
3265 2468 328
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3868 4250 4885 275
Size: 53 83 186 936 2335 382 635 1451
Fragments arranged by size:
2335 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 2242 4494 4892 5166 5313 5724 2242
Size: 2252 398 274 147 411 2579
Fragments arranged by size:
2579 2252 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2015 2045 3260 3290 1178
Size: 30 165 30 612 30 1215 30 3949
Fragments arranged by size:
3949 1215 612 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2334 2383 2700 1186
Size: 1148 49 317 4547
Fragments arranged by size:
4547 1148 317 49
BseYI C'CCAG_C
Cuts at: 2778 2964 5264 5760 2778
Size: 186 2300 496 3079
Fragments arranged by size:
3079 2300 496 186
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5488
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3126 3511 4272 5300 1540
Size: 275 186 1125 385 761 1028 2301
Fragments arranged by size:
2301 1125 1028 761 385 275 186
BsiHKAI G_wGCw'C
Cuts at: 3060 4122 4207 5278 3060
Size: 1062 85 1071 3843
Fragments arranged by size:
3843 1071 1062 85
BsiWI C'GTAC_G
Cuts at: 2070 2070
Size: 6061
BsmI GAATG_Cn'
Cuts at: 3572 3665 3572
Size: 93 5968
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2746 2807 3019 3060 3380 4122 4207
Size: 399 956 61 212 41 320 742 85
Cuts at: 4207 5278 1391
Size: 1071 2174
Fragments arranged by size:
2174 1071 956 742 399 320 212 85
61 41
BspEI T'CCGG_A
Cuts at: 5724 5724
Size: 6061
BspHI T'CATG_A
Cuts at: 3950 5680 3950
Size: 1730 4331
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3416 844
Size: 2572 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 2078 2974 3948 1394
Size: 429 255 896 974 3507
Fragments arranged by size:
3507 974 896 429 255
BsrDI GCAATG_nn'
Cuts at: 65 1248 2429 3096 4556 4730 65
Size: 1183 1181 667 1460 174 1396
Fragments arranged by size:
1460 1396 1183 1181 667 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4705 4892 1700
Size: 110 2895 187 2869
Fragments arranged by size:
2895 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 3138 96
Size: 3042 3019
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6061
BssSI C'ACGA_G
Cuts at: 4121 5133 4121
Size: 1012 5049
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6061
BstBI TT'CG_AA
Cuts at: 4865 4865
Size: 6061
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6061
BstXI CCAn_nnnn'nTGG
Cuts at: 2225 2225
Size: 6061
BstYI r'GATC_y
Cuts at: 1972 2228 2785 2796 2844 3391 3758 4143 4160
Size: 256 557 11 48 547 367 385 17
Cuts at: 4160 5612 5698 5710 6056 1972
Size: 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 557 547 385 367 346 256
86 48 17 12 11
Bsu36I CC'TnA_GG
Cuts at: 2585 2585
Size: 6061
BtgI C'CryG_G
Cuts at: 513 1065 2004 3091 3282 513
Size: 552 939 1087 191 3292
Fragments arranged by size:
3292 1087 939 552 191
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5322
Fragments arranged by size:
5322 683 56
BtsI GCAGTG_nn'
Cuts at: 2597 3577 4371 4391 2597
Size: 980 794 20 4267
Fragments arranged by size:
4267 980 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6061
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6026
DraI TTT'AAA
Cuts at: 3365 3365
Size: 6061
DraIII CAC_nnn'GTG
Cuts at: 2400 2400
Size: 6061
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 3130 5068 809
Size: 507 1814 1938 1802
Fragments arranged by size:
1938 1814 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2955 3508 4397
Size: 54 1425 50 275 18 1125 553 889
Cuts at: 4397 6026 8
Size: 1629 43
Fragments arranged by size:
1629 1425 1125 889 553 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3508 1537
Size: 275 1696 4090
Fragments arranged by size:
4090 1696 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2677 3991 1353
Size: 1324 1314 3423
Fragments arranged by size:
3423 1324 1314
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2298 4648 5022 5168 387
Size: 1064 138 709 2350 374 146 1280
Fragments arranged by size:
2350 1280 1064 709 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2450 2888 3226 4124 4721 5508 5776
Size: 393 818 438 338 898 597 787 268
Cuts at: 5776 1239
Size: 1524
Fragments arranged by size:
1524 898 818 787 597 438 393 338
268
EcoO109I rG'GnC_Cy
Cuts at: 3016 3016
Size: 6061
EcoRI G'AATT_C
Cuts at: 2707 3370 3748 2707
Size: 663 378 5020
Fragments arranged by size:
5020 663 378
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2542 2706 3090 3217 2542
Size: 164 384 127 5386
Fragments arranged by size:
5386 384 164 127
FspI TGC'GCA
Cuts at: 4567 4567
Size: 6061
HaeII r_GCGC'y
Cuts at: 1678 2889 2965 5208 1678
Size: 1211 76 2243 2531
Fragments arranged by size:
2531 2243 1211 76
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2015 2036 2047 2068 2364
Size: 32 135 32 443 21 11 21 296
Cuts at: 2364 2396 2621 2634 2653 2666 2800 2832 4702
Size: 32 225 13 19 13 134 32 1870
Cuts at: 4702 4734 4776 4808 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1870 443 296 225 135 134 42
32 32 32 32 32 32 21 21
19 13 13 11
HincII GTy'rAC
Cuts at: 669 1657 1938 2197 3405 3651 669
Size: 988 281 259 1208 246 3079
Fragments arranged by size:
3079 1208 988 281 259 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6061
HpaI GTT'AAC
Cuts at: 3651 3651
Size: 6061
KpnI G_GTAC'C
Cuts at: 3386 3386
Size: 6061
MfeI C'AATT_G
Cuts at: 3660 3660
Size: 6061
MluI A'CGCG_T
Cuts at: 5937 5937
Size: 6061
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2319 2904 3146 5175 5359 1305
Size: 1014 585 242 2029 184 2007
Fragments arranged by size:
2029 2007 1014 585 242 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2957 10
Size: 54 1425 343 1125 3114
Fragments arranged by size:
3114 1425 1125 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2223 2558 3332 4019 4378 4537 518
Size: 648 1057 335 774 687 359 159 2042
Fragments arranged by size:
2042 1057 774 687 648 359 335 159
MspA1I CmG'CkG
Cuts at: 1606 2851 2898 3477 4154 5302 5547 1606
Size: 1245 47 579 677 1148 245 2120
Fragments arranged by size:
2120 1245 1148 677 579 245 47
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4570
Fragments arranged by size:
4570 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6061
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6061
NotI GC'GGCC_GC
Cuts at: 3508 3508
Size: 6061
NspI r_CATG'y
Cuts at: 1132 1229 2417 3169 3419 4964 1132
Size: 97 1188 752 250 1545 2229
Fragments arranged by size:
2229 1545 1188 752 250 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6061
PciI A'CATG_T
Cuts at: 4960 4960
Size: 6061
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2400 2488 2964 1108
Size: 1292 88 476 4205
Fragments arranged by size:
4205 1292 476 88
PfoI T'CCnGG_A
Cuts at: 2865 2865
Size: 6061
PmeI GTTT'AAAC
Cuts at: 3365 3365
Size: 6061
PmlI CAC'GTG
Cuts at: 3334 3334
Size: 6061
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4130 4162 5643 5675 4130
Size: 32 1481 32 4516
Fragments arranged by size:
4516 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6061
PsiI TTA'TAA
Cuts at: 3631 3631
Size: 6061
PspOMI G'GGCC_C
Cuts at: 2803 3015 2803
Size: 212 5849
PstI C_TGCA'G
Cuts at: 830 2255 3114 3413 830
Size: 1425 859 299 3478
Fragments arranged by size:
3478 1425 859 299
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6061
PvuII CAG'CTG
Cuts at: 2851 2898 2851
Size: 47 6014
SalI G'TCGA_C
Cuts at: 1936 3403 1936
Size: 1467 4594
SbfI CC_TGCA'GG
Cuts at: 3413 3413
Size: 6061
ScaI AGT'ACT
Cuts at: 4309 6021 4309
Size: 1712 4349
SfcI C'TryA_G
Cuts at: 826 1045 2251 3110 3409 4544 5225 5416 826
Size: 219 1206 859 299 1135 681 191 1471
Fragments arranged by size:
1471 1206 1135 859 681 299 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6061
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6061
SmaI CCC'GGG
Cuts at: 1872 3388 1872
Size: 1516 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4165 5066 5328 820
Size: 197 926 2222 901 262 1553
Fragments arranged by size:
2222 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3852 493
Size: 3359 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6061
SphI G_CATG'C
Cuts at: 1132 3419 1132
Size: 2287 3774
SspI AAT'ATT
Cuts at: 5 52 3985 5
Size: 47 3933 2081
Fragments arranged by size:
3933 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6061
StyI C'CwwG_G
Cuts at: 513 1065 2004 2094 2734 3500 513
Size: 552 939 90 640 766 3074
Fragments arranged by size:
3074 939 766 640 552 90
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3140 3344 4088 4105 4258 4443 4888
Size: 1255 1560 204 744 17 153 185 445
Cuts at: 4888 325
Size: 1498
Fragments arranged by size:
1560 1498 1255 744 445 204 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2448 3138 4307
Size: 274 80 33 51 1443 471 690 1169
Cuts at: 4307 6019 96
Size: 1712 138
Fragments arranged by size:
1712 1443 1169 690 471 274 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6021 6053 6021
Size: 32 6029
Tth111I GACn'n_nGTC
Cuts at: 1316 2675 3127 1316
Size: 1359 452 4250
Fragments arranged by size:
4250 1359 452
XbaI T'CTAG_A
Cuts at: 3397 3397
Size: 6061
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2169 2489 2169
Size: 320 5741
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6061
XmaI C'CCGG_G
Cuts at: 1870 3386 1870
Size: 1516 4545
XmnI GAAnn'nnTTC
Cuts at: 4190 4190
Size: 6061
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3869 276
Size: 53 83 186 936 2335 2468
Fragments arranged by size:
2468 2335 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsiWI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I
BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI
EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FalI
FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI
MluI MmeI MscI MslI MspA1I NcoI NdeI NheI
NotI NspI PasI PciI PflMI PfoI PmeI PmlI
PpiI PshAI PsiI PspOMI PstI PvuI PvuII SalI
SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI AscI AvrII BaeI BbeI BbvCI
BfrBI Bpu10I BsmBI BstZ17I EcoICRI EcoNI EcoRV FseI
FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI
PpuMI PspXI PsrI RsrII SacI SacII SanDI SapI
SexAI SfiI SfoI SrfI SwaI