Restriction map for
pFN21AB5545
(Circular) (Six-base) MAPSORT of: pFN21AB5545.seq Check: 5462 from: 1 to: 6577
pFN21AB5545 6577 bp
With 182 enzymes: SgfI *
April 15, 2010 09:35 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 3113 3113
Size: 6577
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4387 278
Size: 53 83 186 936 2851 2468
Fragments arranged by size:
2851 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3541 3920 1937
Size: 1604 379 4594
Fragments arranged by size:
4594 1604 379
Acc65I G'GTAC_C
Cuts at: 3898 3898
Size: 6577
AclI AA'CG_TT
Cuts at: 1563 4704 5077 1563
Size: 3141 373 3063
Fragments arranged by size:
3141 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2253 2613 2889 4640 6024 6292 1632
Size: 621 360 276 1751 1384 268 1917
Fragments arranged by size:
1917 1751 1384 621 360 276 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6380
AflIII A'CryG_T
Cuts at: 1203 5476 6453 1203
Size: 4273 977 1327
Fragments arranged by size:
4273 1327 977
AgeI A'CCGG_T
Cuts at: 5408 5408
Size: 6577
AhdI GACnn_n'nnGTC
Cuts at: 5306 5306
Size: 6577
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6577
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6577
AlwNI CAG_nnn'CTG
Cuts at: 2870 4320 5892 2870
Size: 1450 1572 3555
Fragments arranged by size:
3555 1572 1450
ApaI G_GGCC'C
Cuts at: 3553 3553
Size: 6577
ApaLI G'TGCA_C
Cuts at: 4634 5790 4634
Size: 1156 5421
ApoI r'AATT_y
Cuts at: 1493 2455 2584 2715 3153 3814 3886 4112 4264
Size: 962 129 131 438 661 72 226 152
Cuts at: 4264 6373 1493
Size: 2109 1697
Fragments arranged by size:
2109 1697 962 661 438 226 152 131
129 72
AseI AT'TA_AT
Cuts at: 160 1032 5131 160
Size: 872 4099 1606
Fragments arranged by size:
4099 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6577
AvaI C'yCGr_G
Cuts at: 1870 1943 3902 1870
Size: 73 1959 4545
Fragments arranged by size:
4545 1959 73
BamHI G'GATC_C
Cuts at: 3907 4274 3907
Size: 367 6210
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2165 2802 3524 3898 5353
Size: 325 217 627 378 637 722 374 1455
Cuts at: 5353 618
Size: 1842
Fragments arranged by size:
1842 1455 722 637 627 378 374 325
217
BanII G_rGCy'C
Cuts at: 1391 2651 3553 3896 1391
Size: 1260 902 343 4072
Fragments arranged by size:
4072 1260 902 343
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 5606
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4768 4802 1903
Size: 34 2831 34 3678
Fragments arranged by size:
3678 2831 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4469 5679 4469
Size: 1210 5367
BclI T'GATC_A
Cuts at: 1548 2041 1548
Size: 493 6084
BfrBI ATG'CAT
Cuts at: 3701 3701
Size: 6577
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 5188 136
Size: 107 122 71 631 605 3516 1525
Fragments arranged by size:
3516 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2726 6572 2726
Size: 3846 2731
BlpI GC'TnA_GC
Cuts at: 3994 3994
Size: 6577
Bme1580I G_kGCm'C
Cuts at: 1790 3553 4638 5794 1790
Size: 1763 1085 1156 2573
Fragments arranged by size:
2573 1763 1156 1085
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6577
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3548 4308 5266
Size: 416 53 83 399 24 2131 760 958
Cuts at: 5266 442
Size: 1753
Fragments arranged by size:
2131 1753 958 760 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6577
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 3015 5237 1239
Size: 1776 2222 2579
Fragments arranged by size:
2579 2222 1776
Bpu10I CC'TnA_GC
Cuts at: 3590 3590
Size: 6577
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2874 4702 5567 5865 2874
Size: 1828 865 298 3586
Fragments arranged by size:
3586 1828 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2941 3048 5240 882
Size: 617 1442 107 2192 2219
Fragments arranged by size:
2219 2192 1442 617 107
BsaAI yAC'GTr
Cuts at: 493 1204 4368 493
Size: 711 3164 2702
Fragments arranged by size:
3164 2702 711
BsaBI GATnn'nnATC
Cuts at: 2548 6571 2548
Size: 4023 2554
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4384 4766 5401 275
Size: 53 83 186 936 2851 382 635 1451
Fragments arranged by size:
2851 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 3446 5010 5408 5682 5829 6240 3446
Size: 1564 398 274 147 411 3783
Fragments arranged by size:
3783 1564 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2490 2520 1178
Size: 30 165 30 1087 30 5235
Fragments arranged by size:
5235 1087 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2271 2274 2644 3099 3357 1186
Size: 1085 3 370 455 258 4406
Fragments arranged by size:
4406 1085 455 370 258 3
BseYI C'CCAG_C
Cuts at: 5780 6276 5780
Size: 496 6081
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 6004
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3799 4027 4788 5816 1540
Size: 275 186 1798 228 761 1028 2301
Fragments arranged by size:
2301 1798 1028 761 275 228 186
BsiHKAI G_wGCw'C
Cuts at: 4638 4723 5794 4638
Size: 85 1071 5421
Fragments arranged by size:
5421 1071 85
BsmI GAATG_Cn'
Cuts at: 4088 4181 4088
Size: 93 6484
BsmBI CGTCTCn'nnnn_
Cuts at: 2194 2771 2194
Size: 577 6000
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2651 3553 3896 4638 4723 5794 1391
Size: 399 861 902 343 742 85 1071 2174
Fragments arranged by size:
2174 1071 902 861 742 399 343 85
BspEI T'CCGG_A
Cuts at: 6240 6240
Size: 6577
BspHI T'CATG_A
Cuts at: 4466 6196 4466
Size: 1730 4847
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2888 3113 3932 844
Size: 2044 225 819 3489
Fragments arranged by size:
3489 2044 819 225
BsrBI CCG'CTC
Cuts at: 1394 1823 3118 4464 1394
Size: 429 1295 1346 3507
Fragments arranged by size:
3507 1346 1295 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3681 5072 5246 65
Size: 1183 2433 1391 174 1396
Fragments arranged by size:
2433 1396 1391 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 5221 5408 1700
Size: 110 3411 187 2869
Fragments arranged by size:
3411 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 3264 96
Size: 3168 3409
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6577
BssSI C'ACGA_G
Cuts at: 4637 5649 4637
Size: 1012 5565
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6577
BstBI TT'CG_AA
Cuts at: 5381 5381
Size: 6577
BstEII G'GTnAC_C
Cuts at: 1184 2691 1184
Size: 1507 5070
BstYI r'GATC_y
Cuts at: 1972 2726 3907 4274 4659 4676 6128 6214 6226
Size: 754 1181 367 385 17 1452 86 12
Cuts at: 6226 6572 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 1181 754 385 367 346 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 513
Size: 552 939 5086
Fragments arranged by size:
5086 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5838
Fragments arranged by size:
5838 683 56
BtsI GCAGTG_nn'
Cuts at: 2382 3787 4093 4887 4907 2382
Size: 1405 306 794 20 4052
Fragments arranged by size:
4052 1405 794 306 20
ClaI AT'CG_AT
Cuts at: 1554 3795 1554
Size: 2241 4336
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2017 2052 529
Size: 35 1453 35 5054
Fragments arranged by size:
5054 1453 35 35
DraI TTT'AAA
Cuts at: 3881 3881
Size: 6577
DraIII CAC_nnn'GTG
Cuts at: 2809 3335 2809
Size: 526 6051
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5584 809
Size: 507 4268 1802
Fragments arranged by size:
4268 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 4024 4913 6542
Size: 54 1425 50 275 18 2194 889 1629
Cuts at: 6542 8
Size: 43
Fragments arranged by size:
2194 1629 1425 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 4024 1537
Size: 275 2212 4090
Fragments arranged by size:
4090 2212 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2184 2272 2977 3334 4507 1353
Size: 831 88 705 357 1173 3423
Fragments arranged by size:
3423 1173 831 705 357 88
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5164 5538 5684 387
Size: 1064 138 3575 374 146 1280
Fragments arranged by size:
3575 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2253 2613 2889 3015 4640 5237 6024
Size: 393 621 360 276 126 1625 597 787
Cuts at: 6024 6292 1239
Size: 268 1524
Fragments arranged by size:
1625 1524 787 621 597 393 360 276
268 126
EcoNI CCTnn'n_nnAGG
Cuts at: 2619 2619
Size: 6577
EcoO109I rG'GnC_Cy
Cuts at: 2608 3549 3550 2608
Size: 941 1 5635
Fragments arranged by size:
5635 941 1
EcoRI G'AATT_C
Cuts at: 3153 3814 3886 4264 3153
Size: 661 72 378 5466
Fragments arranged by size:
5466 661 378 72
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2901 3594 2901
Size: 693 5884
FspI TGC'GCA
Cuts at: 5083 5083
Size: 6577
HaeII r_GCGC'y
Cuts at: 1678 5724 1678
Size: 4046 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2264 2296 3182 3214 3263
Size: 32 135 32 692 32 886 32 49
Cuts at: 3263 3295 5218 5250 5292 5324 1373
Size: 32 1923 32 42 32 2626
Fragments arranged by size:
2626 1923 886 692 135 49 42 32
32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2434 2601 3542 3921 4167 669
Size: 988 281 496 167 941 379 246 3079
Fragments arranged by size:
3079 988 941 496 379 281 246 167
HindIII A'AGCT_T
Cuts at: 748 2094 748
Size: 1346 5231
HpaI GTT'AAC
Cuts at: 2434 2601 4167 2434
Size: 167 1566 4844
Fragments arranged by size:
4844 1566 167
KpnI G_GTAC'C
Cuts at: 3902 3902
Size: 6577
MfeI C'AATT_G
Cuts at: 4176 4176
Size: 6577
MluI A'CGCG_T
Cuts at: 6453 6453
Size: 6577
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2363 2978 5691 5875 1305
Size: 1058 615 2713 184 2007
Fragments arranged by size:
2713 2007 1058 615 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4755
Fragments arranged by size:
4755 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 4535 4894 5053 518
Size: 648 3369 359 159 2042
Fragments arranged by size:
3369 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2915 3993 4670 5818 6063 1606
Size: 1309 1078 677 1148 245 2120
Fragments arranged by size:
2120 1309 1148 1078 677 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 5086
Fragments arranged by size:
5086 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6577
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6577
NotI GC'GGCC_GC
Cuts at: 4024 4024
Size: 6577
NsiI A_TGCA'T
Cuts at: 3703 3703
Size: 6577
NspI r_CATG'y
Cuts at: 1132 1229 3935 5480 1132
Size: 97 2706 1545 2229
Fragments arranged by size:
2706 2229 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6577
PciI A'CATG_T
Cuts at: 5476 5476
Size: 6577
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2399 1108
Size: 1291 5286
PfoI T'CCnGG_A
Cuts at: 2992 2992
Size: 6577
PmeI GTTT'AAAC
Cuts at: 3881 3881
Size: 6577
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3373 3391 3405 3423 4646 4678 6159 6191 3373
Size: 18 14 18 1223 32 1481 32 3759
Fragments arranged by size:
3759 1481 1223 32 32 18 18 14
PpuMI rG'GwC_Cy
Cuts at: 2608 2608
Size: 6577
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6577
PsiI TTA'TAA
Cuts at: 4147 4147
Size: 6577
PspOMI G'GGCC_C
Cuts at: 3549 3549
Size: 6577
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 2970 3002 3355 3387 2970
Size: 32 353 32 6160
Fragments arranged by size:
6160 353 32 32
PstI C_TGCA'G
Cuts at: 830 2053 3929 830
Size: 1223 1876 3478
Fragments arranged by size:
3478 1876 1223
PvuI CG_AT'CG
Cuts at: 2001 3799 2001
Size: 1798 4779
PvuII CAG'CTG
Cuts at: 2915 2915
Size: 6577
SalI G'TCGA_C
Cuts at: 1936 3540 3919 1936
Size: 1604 379 4594
Fragments arranged by size:
4594 1604 379
SbfI CC_TGCA'GG
Cuts at: 3929 3929
Size: 6577
ScaI AGT'ACT
Cuts at: 2182 4825 6537 2182
Size: 2643 1712 2222
Fragments arranged by size:
2643 2222 1712
SfcI C'TryA_G
Cuts at: 826 1045 2049 2207 2931 3712 3925 5060 5741
Size: 219 1004 158 724 781 213 1135 681
Cuts at: 5741 5932 826
Size: 191 1471
Fragments arranged by size:
1471 1135 1004 781 724 681 219 213
191 158
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6577
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6577
SmaI CCC'GGG
Cuts at: 1872 3904 1872
Size: 2032 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2889 4681 5582 5844 820
Size: 197 926 946 1792 901 262 1553
Fragments arranged by size:
1792 1553 946 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 4368 493
Size: 3875 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6577
SphI G_CATG'C
Cuts at: 1132 3935 1132
Size: 2803 3774
SspI AAT'ATT
Cuts at: 5 52 4501 5
Size: 47 4449 2081
Fragments arranged by size:
4449 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6577
StyI C'CwwG_G
Cuts at: 513 1065 2004 4016 513
Size: 552 939 2012 3074
Fragments arranged by size:
3074 2012 939 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2091 3024 4604 4621 4774 4959 5404
Size: 1255 511 933 1580 17 153 185 445
Cuts at: 5404 325
Size: 1498
Fragments arranged by size:
1580 1498 1255 933 511 445 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2180 3264 4823
Size: 274 80 33 51 1443 203 1084 1559
Cuts at: 4823 6535 96
Size: 1712 138
Fragments arranged by size:
1712 1559 1443 1084 274 203 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2490 2522 6537 6569 2490
Size: 32 4015 32 2498
Fragments arranged by size:
4015 2498 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6577
XbaI T'CTAG_A
Cuts at: 2570 2792 3913 2570
Size: 222 1121 5234
Fragments arranged by size:
5234 1121 222
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6577
XmaI C'CCGG_G
Cuts at: 1870 3902 1870
Size: 2032 4545
XmnI GAAnn'nnTTC
Cuts at: 2884 4706 2884
Size: 1822 4755
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4385 276
Size: 53 83 186 936 2851 2468
Fragments arranged by size:
2851 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI
BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI
BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI
EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI
FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI
MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI
NheI NotI NsiI NspI PasI PciI PflMI PfoI
PmeI PpiI PpuMI PshAI PsiI PspOMI PsrI PstI
PvuI PvuII SalI SbfI ScaI SfcI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AfeI AloI AscI AvrII BaeI BbeI BbvCI BplI
BsiWI BstXI BstZ17I Bsu36I EcoICRI EcoRV FseI FspAI
KasI NaeI NarI NgoMIV NruI PacI PmlI PspXI
RsrII SacI SacII SanDI SapI SexAI SfiI SfoI
SrfI SwaI XcmI