Restriction map for
pFN21AB5544
(Circular) (Six-base) MAPSORT of: pFN21AB5544.seq Check: 5443 from: 1 to: 9040
pFN21AB5544 9040 bp
With 182 enzymes: SgfI *
January 16, 2009 15:25 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 3085 3085
Size: 9040
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3449 6850 278
Size: 53 83 186 936 1913 3401 2468
Fragments arranged by size:
3401 2468 1913 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2419 4793 6273 6383 1937
Size: 482 2374 1480 110 4594
Fragments arranged by size:
4594 2374 1480 482 110
Acc65I G'GTAC_C
Cuts at: 6361 6361
Size: 9040
AclI AA'CG_TT
Cuts at: 1563 7167 7540 1563
Size: 5604 373 3063
Fragments arranged by size:
5604 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2151 3387 4149 4964 5300 5400 5535 7103
Size: 519 1236 762 815 336 100 135 1568
Cuts at: 7103 8487 8755 1632
Size: 1384 268 1917
Fragments arranged by size:
1917 1568 1384 1236 815 762 519 336
268 135 100
AfeI AGC'GCT
Cuts at: 4574 4574
Size: 9040
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 8843
AflIII A'CryG_T
Cuts at: 1203 7939 8916 1203
Size: 6736 977 1327
Fragments arranged by size:
6736 1327 977
AgeI A'CCGG_T
Cuts at: 7871 7871
Size: 9040
AhdI GACnn_n'nnGTC
Cuts at: 2332 7769 2332
Size: 5437 3603
AleI CACnn'nnGTG
Cuts at: 1166 2782 4028 4515 5184 1166
Size: 1616 1246 487 669 5022
Fragments arranged by size:
5022 1616 1246 669 487
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2159 5202 1184
Size: 975 3043 5022
Fragments arranged by size:
5022 3043 975
AlwNI CAG_nnn'CTG
Cuts at: 2308 2938 3076 6115 6314 6783 8355 2308
Size: 630 138 3039 199 469 1572 2993
Fragments arranged by size:
3039 2993 1572 630 469 199 138
ApaI G_GGCC'C
Cuts at: 2116 3497 3696 5168 2116
Size: 1381 199 1472 5988
Fragments arranged by size:
5988 1472 1381 199
ApaLI G'TGCA_C
Cuts at: 5177 7097 8253 5177
Size: 1920 1156 5964
Fragments arranged by size:
5964 1920 1156
ApoI r'AATT_y
Cuts at: 1493 2450 5660 6349 6575 6727 8836 1493
Size: 957 3210 689 226 152 2109 1697
Fragments arranged by size:
3210 2109 1697 957 689 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4725 5622 7594 160
Size: 872 3693 897 1972 1606
Fragments arranged by size:
3693 1972 1606 897 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 9040
AvaI C'yCGr_G
Cuts at: 1870 1943 3427 4428 5099 5574 6365 1870
Size: 73 1484 1001 671 475 791 4545
Fragments arranged by size:
4545 1484 1001 791 671 475 73
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 2749 2782 5367 5400 2749
Size: 33 2585 33 6389
Fragments arranged by size:
6389 2585 33 33
BamHI G'GATC_C
Cuts at: 2647 2704 6370 6737 2647
Size: 57 3666 367 4950
Fragments arranged by size:
4950 3666 367 57
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2195 3731 5207 6361 7816
Size: 325 217 627 408 1536 1476 1154 1455
Cuts at: 7816 618
Size: 1842
Fragments arranged by size:
1842 1536 1476 1455 1154 627 408 325
217
BanII G_rGCy'C
Cuts at: 1391 2116 2486 3497 3696 3873 4524 5168 6359
Size: 725 370 1011 199 177 651 644 1191
Cuts at: 6359 1391
Size: 4072
Fragments arranged by size:
4072 1191 1011 725 651 644 370 199
177
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2328 2803 3197 3426 4137 4419 4882
Size: 971 429 475 394 229 711 282 463
Cuts at: 4882 928
Size: 5086
Fragments arranged by size:
5086 971 711 475 463 429 394 282
229
BbvCI CC'TCA_GC
Cuts at: 3631 5140 3631
Size: 1509 7531
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 3532 3566 7231 7265 1903
Size: 34 50 34 1511 34 3665 34 3678
Fragments arranged by size:
3678 3665 1511 50 34 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 6932 8142 6932
Size: 1210 7830
BclI T'GATC_A
Cuts at: 1548 4663 4888 6159 1548
Size: 3115 225 1271 4429
Fragments arranged by size:
4429 3115 1271 225
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 3413 3524 7651
Size: 107 122 71 631 605 1741 111 4127
Cuts at: 7651 136
Size: 1525
Fragments arranged by size:
4127 1741 1525 631 605 122 111 107
71
BglII A'GATC_T
Cuts at: 9035 9035
Size: 9040
BlpI GC'TnA_GC
Cuts at: 2476 6227 6457 2476
Size: 3751 230 5059
Fragments arranged by size:
5059 3751 230
Bme1580I G_kGCm'C
Cuts at: 1790 2116 3384 3497 3696 3734 4035 5168 5181
Size: 326 1268 113 199 38 301 1133 13
Cuts at: 5181 6094 7101 8257 1790
Size: 913 1007 1156 2573
Fragments arranged by size:
2573 1268 1156 1133 1007 913 326 301
199 113 38 13
BmgBI CAC'GTC
Cuts at: 1318 2223 1318
Size: 905 8135
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2191 2224 2467
Size: 416 53 83 399 24 774 33 243
Cuts at: 2467 3026 3495 6771 7729 442
Size: 559 469 3276 958 1753
Fragments arranged by size:
3276 1753 958 774 559 469 416 399
243 83 53 33 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 9040
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3826 3826
Size: 9040
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 3124 3487 3672 4182 4309 4831 6137 6198
Size: 1885 363 185 510 127 522 1306 61
Cuts at: 6198 7700 1239
Size: 1502 2579
Fragments arranged by size:
2579 1885 1502 1306 522 510 363 185
127 61
Bpu10I CC'TnA_GC
Cuts at: 2208 3631 5140 2208
Size: 1423 1509 6108
Fragments arranged by size:
6108 1509 1423
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2867 7165 8030 8328 2867
Size: 4298 865 298 3579
Fragments arranged by size:
4298 3579 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2687 2812 7703 882
Size: 617 1188 125 4891 2219
Fragments arranged by size:
4891 2219 1188 617 125
BsaAI yAC'GTr
Cuts at: 493 1204 6831 493
Size: 711 5627 2702
Fragments arranged by size:
5627 2702 711
BsaBI GATnn'nnATC
Cuts at: 9034 9034
Size: 9040
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3446 6847 7229 7864
Size: 53 83 186 936 1913 3401 382 635
Cuts at: 7864 275
Size: 1451
Fragments arranged by size:
3401 1913 1451 936 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 2814 4468 7473 7871 8145 8292 8703 2814
Size: 1654 3005 398 274 147 411 3151
Fragments arranged by size:
3151 3005 1654 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 3137 3167 3179 3209 3376
Size: 30 165 30 1734 30 12 30 167
Cuts at: 3376 3406 4282 4312 1178
Size: 30 876 30 5906
Fragments arranged by size:
5906 1734 876 167 165 30 30 30
30 30 30 12
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 3490 3574 3806 3866 4064 4377 5146 5336
Size: 2304 84 232 60 198 313 769 190
Cuts at: 5336 1186
Size: 4890
Fragments arranged by size:
4890 2304 769 313 232 198 190 84
60
BseYI C'CCAG_C
Cuts at: 3593 3872 6108 8243 8739 3593
Size: 279 2236 2135 496 3894
Fragments arranged by size:
3894 2236 2135 496 279
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2102 2165 2625 3089 5384 1163
Size: 573 366 63 460 464 2295 4819
Fragments arranged by size:
4819 2295 573 464 460 366 63
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3627 6490 7251 8279 1540
Size: 275 186 1626 2863 761 1028 2301
Fragments arranged by size:
2863 2301 1626 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2067 2214 3789 4281 4524 4952 5181 5515 5575
Size: 147 1575 492 243 428 229 334 60
Cuts at: 5575 7101 7186 8257 2067
Size: 1526 85 1071 2850
Fragments arranged by size:
2850 1575 1526 1071 492 428 334 243
229 147 85 60
BsmI GAATG_Cn'
Cuts at: 4606 6551 6644 4606
Size: 1945 93 7002
Fragments arranged by size:
7002 1945 93
BsmBI CGTCTCn'nnnn_
Cuts at: 3396 3894 3396
Size: 498 8542
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2067 2116 2214 2486 3384 3497 3696
Size: 399 277 49 98 272 898 113 199
Cuts at: 3696 3734 3789 3873 4035 4281 4524 4952 5168
Size: 38 55 84 162 246 243 428 216
Cuts at: 5168 5181 5515 5575 6094 6359 7101 7186 8257
Size: 13 334 60 519 265 742 85 1071
Cuts at: 8257 1391
Size: 2174
Fragments arranged by size:
2174 1071 898 742 519 428 399 334
277 272 265 246 243 216 199 162
113 98 85 84 60 55 49 38
13
BspEI T'CCGG_A
Cuts at: 2814 4468 8703 2814
Size: 1654 4235 3151
Fragments arranged by size:
4235 3151 1654
BspHI T'CATG_A
Cuts at: 2683 3138 6052 6929 8659 2683
Size: 455 2914 877 1730 3064
Fragments arranged by size:
3064 2914 1730 877 455
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3085 6395 844
Size: 2241 3310 3489
Fragments arranged by size:
3489 3310 2241
BsrBI CCG'CTC
Cuts at: 1394 1823 3781 3856 6927 1394
Size: 429 1958 75 3071 3507
Fragments arranged by size:
3507 3071 1958 429 75
BsrDI GCAATG_nn'
Cuts at: 65 1248 2476 4269 4767 5591 5662 6228 7535
Size: 1183 1228 1793 498 824 71 566 1307
Cuts at: 7535 7709 65
Size: 174 1396
Fragments arranged by size:
1793 1396 1307 1228 1183 824 566 498
174 71
BsrFI r'CCGG_y
Cuts at: 1700 1810 3554 3989 7684 7871 1700
Size: 110 1744 435 3695 187 2869
Fragments arranged by size:
3695 2869 1744 435 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 9040
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 9040
BssSI C'ACGA_G
Cuts at: 2120 3788 7100 8112 2120
Size: 1668 3312 1012 3048
Fragments arranged by size:
3312 3048 1668 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2938 5683 794
Size: 2144 2745 4151
Fragments arranged by size:
4151 2745 2144
BstBI TT'CG_AA
Cuts at: 7844 7844
Size: 9040
BstEII G'GTnAC_C
Cuts at: 1184 3535 3835 4015 1184
Size: 2351 300 180 6209
Fragments arranged by size:
6209 2351 300 180
BstXI CCAn_nnnn'nTGG
Cuts at: 2471 2471
Size: 9040
BstYI r'GATC_y
Cuts at: 1972 2148 2647 2704 5135 6370 6737 7122 7139
Size: 176 499 57 2431 1235 367 385 17
Cuts at: 7139 8591 8677 8689 9035 1972
Size: 1452 86 12 346 1977
Fragments arranged by size:
2431 1977 1452 1235 499 385 367 346
176 86 57 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2601 2659 2871 3477 4111 513
Size: 552 1536 58 212 606 634 5442
Fragments arranged by size:
5442 1536 634 606 552 212 58
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 8301
Fragments arranged by size:
8301 683 56
BtsI GCAGTG_nn'
Cuts at: 2148 3089 3948 4596 5179 5191 6556 7350 7370
Size: 941 859 648 583 12 1365 794 20
Cuts at: 7370 2148
Size: 3818
Fragments arranged by size:
3818 1365 941 859 794 648 583 20
12
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 9040
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 5478 5513 529
Size: 35 4914 35 4056
Fragments arranged by size:
4914 4056 35 35
DraI TTT'AAA
Cuts at: 6344 6344
Size: 9040
DraIII CAC_nnn'GTG
Cuts at: 3603 5481 3603
Size: 1878 7162
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2402 8047 809
Size: 507 1086 5645 1802
Fragments arranged by size:
5645 1802 1086 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2604 3624 4898
Size: 54 1425 50 275 18 774 1020 1274
Cuts at: 4898 5792 6487 7376 9005 8
Size: 894 695 889 1629 43
Fragments arranged by size:
1629 1425 1274 1020 894 889 774 695
275 54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3624 6487 1537
Size: 275 1812 2863 4090
Fragments arranged by size:
4090 2863 1812 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3406 3821 4940 5554 6012 6970 1353
Size: 2053 415 1119 614 458 958 3423
Fragments arranged by size:
3423 2053 1119 958 614 458 415
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2953 3990 7627 8001 8147 387
Size: 1064 138 1364 1037 3637 374 146 1280
Fragments arranged by size:
3637 1364 1280 1064 1037 374 146 138
EcoICRI GAG'CTC
Cuts at: 4522 4522
Size: 9040
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2151 3124 3387 3487 3672 4149 4182
Size: 393 519 973 263 100 185 477 33
Cuts at: 4182 4309 4831 4964 5300 5400 5535 6137 6198
Size: 127 522 133 336 100 135 602 61
Cuts at: 6198 7103 7700 8487 8755 1239
Size: 905 597 787 268 1524
Fragments arranged by size:
1524 973 905 787 602 597 522 519
477 393 336 268 263 185 135 133
127 100 100 61 33
EcoNI CCTnn'n_nnAGG
Cuts at: 4197 4692 6028 4197
Size: 495 1336 7209
Fragments arranged by size:
7209 1336 495
EcoO109I rG'GnC_Cy
Cuts at: 2112 2113 2722 3494 3692 4781 5165 2112
Size: 1 609 772 198 1089 384 5987
Fragments arranged by size:
5987 1089 772 609 384 198 1
EcoRI G'AATT_C
Cuts at: 6349 6727 6349
Size: 378 8662
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2713 5381 6242 2713
Size: 2668 861 5511
Fragments arranged by size:
5511 2668 861
FspI TGC'GCA
Cuts at: 3299 7546 3299
Size: 4247 4793
HaeII r_GCGC'y
Cuts at: 1678 4576 8187 1678
Size: 2898 3611 2531
Fragments arranged by size:
3611 2898 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2318 2350 4207 4239 5050
Size: 32 135 32 746 32 1857 32 811
Cuts at: 5050 5082 5707 5739 5847 5879 7681 7713 7755
Size: 32 625 32 108 32 1802 32 42
Cuts at: 7755 7787 1373
Size: 32 2626
Fragments arranged by size:
2626 1857 1802 811 746 625 135 108
42 32 32 32 32 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2094 5263 5413 6384 6630 669
Size: 988 281 156 3169 150 971 246 3079
Fragments arranged by size:
3169 3079 988 971 281 246 156 150
HindIII A'AGCT_T
Cuts at: 748 748
Size: 9040
HpaI GTT'AAC
Cuts at: 5263 5413 6630 5263
Size: 150 1217 7673
Fragments arranged by size:
7673 1217 150
KpnI G_GTAC'C
Cuts at: 6365 6365
Size: 9040
MfeI C'AATT_G
Cuts at: 6639 6639
Size: 9040
MluI A'CGCG_T
Cuts at: 8916 8916
Size: 9040
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3266 5334 8154 8338 1305
Size: 1961 2068 2820 184 2007
Fragments arranged by size:
2820 2068 2007 1961 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2606 4900 5794 10
Size: 54 1425 343 774 2294 894 3256
Fragments arranged by size:
3256 2294 1425 894 774 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2469 2782 3059 3065 4028 4274 4404
Size: 648 1303 313 277 6 963 246 130
Cuts at: 4404 4515 4847 5184 5406 5744 6998 7357 7516
Size: 111 332 337 222 338 1254 359 159
Cuts at: 7516 518
Size: 2042
Fragments arranged by size:
2042 1303 1254 963 648 359 338 337
332 313 277 246 222 159 130 111
6
MspA1I CmG'CkG
Cuts at: 1606 2235 2480 3162 4113 4160 5398 6456 7133
Size: 629 245 682 951 47 1238 1058 677
Cuts at: 7133 8281 8526 1606
Size: 1148 245 2120
Fragments arranged by size:
2120 1238 1148 1058 951 682 677 629
245 245 47
NaeI GCC'GGC
Cuts at: 3556 3991 3556
Size: 435 8605
NcoI C'CATG_G
Cuts at: 513 1065 2601 3477 513
Size: 552 1536 876 6076
Fragments arranged by size:
6076 1536 876 552
NdeI CA'TA_TG
Cuts at: 387 4660 387
Size: 4273 4767
NgoMIV G'CCGG_C
Cuts at: 3554 3989 3554
Size: 435 8605
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 9040
NotI GC'GGCC_GC
Cuts at: 6487 6487
Size: 9040
NspI r_CATG'y
Cuts at: 1132 1229 3298 4964 5233 6398 7943 1132
Size: 97 2069 1666 269 1165 1545 2229
Fragments arranged by size:
2229 2069 1666 1545 1165 269 97
PasI CC'CwG_GG
Cuts at: 1348 2740 1348
Size: 1392 7648
PciI A'CATG_T
Cuts at: 7939 7939
Size: 9040
PflMI CCAn_nnn'nTGG
Cuts at: 1108 5597 5767 6115 1108
Size: 4489 170 348 4033
Fragments arranged by size:
4489 4033 348 170
PfoI T'CCnGG_A
Cuts at: 2718 2978 3101 2718
Size: 260 123 8657
Fragments arranged by size:
8657 260 123
PmeI GTTT'AAAC
Cuts at: 6344 6344
Size: 9040
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2298 2330 5531 5563 7109 7141 8622 8654 2298
Size: 32 3201 32 1546 32 1481 32 2684
Fragments arranged by size:
3201 2684 1546 1481 32 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 2722 4781 2722
Size: 2059 6981
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 9040
PsiI TTA'TAA
Cuts at: 5669 6610 5669
Size: 941 8099
PspOMI G'GGCC_C
Cuts at: 2112 3493 3692 5164 2112
Size: 1381 199 1472 5988
Fragments arranged by size:
5988 1472 1381 199
PspXI vC'TCGA_Gb
Cuts at: 4428 5574 4428
Size: 1146 7894
PstI C_TGCA'G
Cuts at: 830 3096 4603 5186 6392 830
Size: 2266 1507 583 1206 3478
Fragments arranged by size:
3478 2266 1507 1206 583
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 9040
PvuII CAG'CTG
Cuts at: 2235 3162 5398 2235
Size: 927 2236 5877
Fragments arranged by size:
5877 2236 927
SacI G_AGCT'C
Cuts at: 4524 4524
Size: 9040
SacII CC_GC'GG
Cuts at: 4114 4114
Size: 9040
SalI G'TCGA_C
Cuts at: 1936 6382 1936
Size: 4446 4594
SapI GCTCTTCn'nnn_
Cuts at: 4940 4940
Size: 9040
SbfI CC_TGCA'GG
Cuts at: 6392 6392
Size: 9040
ScaI AGT'ACT
Cuts at: 5118 7288 9000 5118
Size: 2170 1712 5158
Fragments arranged by size:
5158 2170 1712
SfcI C'TryA_G
Cuts at: 826 1045 3092 3157 4599 5182 6388 7523 8204
Size: 219 2047 65 1442 583 1206 1135 681
Cuts at: 8204 8395 826
Size: 191 1471
Fragments arranged by size:
2047 1471 1442 1206 1135 681 583 219
191 65
SfiI GGCCn_nnn'nGGCC
Cuts at: 3524 3524
Size: 9040
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 9040
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 9040
SmaI CCC'GGG
Cuts at: 1872 6367 1872
Size: 4495 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2846 4428 5574 7144 8045 8307
Size: 197 926 903 1582 1146 1570 901 262
Cuts at: 8307 820
Size: 1553
Fragments arranged by size:
1582 1570 1553 1146 926 903 901 262
197
SnaBI TAC'GTA
Cuts at: 493 6831 493
Size: 6338 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 9040
SphI G_CATG'C
Cuts at: 1132 5233 6398 1132
Size: 4101 1165 3774
Fragments arranged by size:
4101 3774 1165
SspI AAT'ATT
Cuts at: 5 52 3348 6964 5
Size: 47 3296 3616 2081
Fragments arranged by size:
3616 3296 2081 47
StuI AGG'CCT
Cuts at: 1517 3677 3999 5825 6023 6175 1517
Size: 2160 322 1826 198 152 4382
Fragments arranged by size:
4382 2160 1826 322 198 152
StyI C'CwwG_G
Cuts at: 513 1065 2601 3352 3477 3678 3717 4314 4777
Size: 552 1536 751 125 201 39 597 463
Cuts at: 4777 5031 6479 513
Size: 254 1448 3074
Fragments arranged by size:
3074 1536 1448 751 597 552 463 254
201 125 39
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3067 3144 3148 4040 4134 4790 7067
Size: 1255 1487 77 4 892 94 656 2277
Cuts at: 7067 7084 7237 7422 7867 325
Size: 17 153 185 445 1498
Fragments arranged by size:
2277 1498 1487 1255 892 656 445 185
153 94 77 17 4
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4577 5116 5328
Size: 274 80 33 51 1443 2600 539 212
Cuts at: 5328 5787 7286 8998 96
Size: 459 1499 1712 138
Fragments arranged by size:
2600 1712 1499 1443 539 459 274 212
138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3376 3408 4138 4170 4830 4862 9000 9032 3376
Size: 32 730 32 660 32 4138 32 3384
Fragments arranged by size:
4138 3384 730 660 32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 2888 4013 5924 1316
Size: 1572 1125 1911 4432
Fragments arranged by size:
4432 1911 1572 1125
XbaI T'CTAG_A
Cuts at: 6376 6376
Size: 9040
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3676 3676
Size: 9040
XhoI C'TCGA_G
Cuts at: 1943 4428 5574 1943
Size: 2485 1146 5409
Fragments arranged by size:
5409 2485 1146
XmaI C'CCGG_G
Cuts at: 1870 6365 1870
Size: 4495 4545
XmnI GAAnn'nnTTC
Cuts at: 4449 4485 5361 5548 5702 7169 4449
Size: 36 876 187 154 1467 6320
Fragments arranged by size:
6320 1467 876 187 154 36
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3447 6848 276
Size: 53 83 186 936 1913 3401 2468
Fragments arranged by size:
3401 2468 1913 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII
BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI
Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI
BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI
CspCI DraI DraIII DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII
Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI
MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI
NotI NspI PasI PciI PflMI PfoI PmeI PpiI
PpuMI PshAI PsiI PspOMI PspXI PstI PvuI PvuII
SacI SacII SalI SapI SbfI ScaI SfcI SfiI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XcmI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AloI AscI AvrII BbeI BfrBI BsiWI BstZ17I Bsu36I
EcoRV FseI FspAI KasI NarI NruI NsiI PacI
PmlI PsrI RsrII SanDI SexAI SfoI SrfI SwaI