Restriction map for
pFN21AB5543
(Circular) (Six-base) MAPSORT of: pFN21AB5543.seq Check: 9615 from: 1 to: 5743
pFN21AB5543 5743 bp
With 182 enzymes: SgfI *
April 15, 2010 09:31 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3553 278
Size: 53 83 186 936 2017 2468
Fragments arranged by size:
2468 2017 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3086 1937
Size: 1149 4594
Acc65I G'GTAC_C
Cuts at: 3064 3064
Size: 5743
AclI AA'CG_TT
Cuts at: 1563 3870 4243 1563
Size: 2307 373 3063
Fragments arranged by size:
3063 2307 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3806 5190 5458 1632
Size: 2174 1384 268 1917
Fragments arranged by size:
2174 1917 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5546
AflIII A'CryG_T
Cuts at: 1203 4642 5619 1203
Size: 3439 977 1327
Fragments arranged by size:
3439 1327 977
AgeI A'CCGG_T
Cuts at: 4574 4574
Size: 5743
AhdI GACnn_n'nnGTC
Cuts at: 4472 4472
Size: 5743
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5743
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5743
AlwNI CAG_nnn'CTG
Cuts at: 2774 3486 5058 2774
Size: 712 1572 3459
Fragments arranged by size:
3459 1572 712
ApaLI G'TGCA_C
Cuts at: 3800 4956 3800
Size: 1156 4587
ApoI r'AATT_y
Cuts at: 1493 2687 2900 3052 3278 3430 5539 1493
Size: 1194 213 152 226 152 2109 1697
Fragments arranged by size:
2109 1697 1194 226 213 152 152
AseI AT'TA_AT
Cuts at: 160 1032 4297 160
Size: 872 3265 1606
Fragments arranged by size:
3265 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5743
AvaI C'yCGr_G
Cuts at: 1870 1943 3068 1870
Size: 73 1125 4545
Fragments arranged by size:
4545 1125 73
BamHI G'GATC_C
Cuts at: 3073 3440 3073
Size: 367 5376
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3064 4519 618
Size: 325 217 627 1277 1455 1842
Fragments arranged by size:
1842 1455 1277 627 325 217
BanII G_rGCy'C
Cuts at: 1391 3062 1391
Size: 1671 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2442 928
Size: 971 543 4229
Fragments arranged by size:
4229 971 543
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3934 3968 1903
Size: 34 1997 34 3678
Fragments arranged by size:
3678 1997 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3635 4845 3635
Size: 1210 4533
BclI T'GATC_A
Cuts at: 1548 2274 1548
Size: 726 5017
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4354 136
Size: 107 122 71 631 605 2682 1525
Fragments arranged by size:
2682 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2314 5738 2314
Size: 3424 2319
BlpI GC'TnA_GC
Cuts at: 3160 3160
Size: 5743
Bme1580I G_kGCm'C
Cuts at: 1790 3804 4960 1790
Size: 2014 1156 2573
Fragments arranged by size:
2573 2014 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5743
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2784 3474 4432
Size: 416 53 83 399 24 1367 690 958
Cuts at: 4432 442
Size: 1753
Fragments arranged by size:
1753 1367 958 690 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5743
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2059 2172 2748 2754 4403 1239
Size: 820 113 576 6 1649 2579
Fragments arranged by size:
2579 1649 820 576 113 6
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2442 3868 4733 5031 2442
Size: 1426 865 298 3154
Fragments arranged by size:
3154 1426 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4406 882
Size: 617 2907 2219
Fragments arranged by size:
2907 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3534 493
Size: 711 2330 2702
Fragments arranged by size:
2702 2330 711
BsaBI GATnn'nnATC
Cuts at: 5737 5737
Size: 5743
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3550 3932 4567 275
Size: 53 83 186 936 2017 382 635 1451
Fragments arranged by size:
2017 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4176 4574 4848 4995 5406 4176
Size: 398 274 147 411 4513
Fragments arranged by size:
4513 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5518
Fragments arranged by size:
5518 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2853 1186
Size: 1667 4076
BseYI C'CCAG_C
Cuts at: 4946 5442 4946
Size: 496 5247
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5170
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3193 3954 4982 1540
Size: 275 186 1192 761 1028 2301
Fragments arranged by size:
2301 1192 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3804 3889 4960 3804
Size: 85 1071 4587
Fragments arranged by size:
4587 1071 85
BsmI GAATG_Cn'
Cuts at: 2122 3254 3347 2122
Size: 1132 93 4518
Fragments arranged by size:
4518 1132 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 3062 3804 3889 4960 1391
Size: 399 1272 742 85 1071 2174
Fragments arranged by size:
2174 1272 1071 742 399 85
BspEI T'CCGG_A
Cuts at: 5406 5406
Size: 5743
BspHI T'CATG_A
Cuts at: 2614 3632 5362 2614
Size: 1018 1730 2995
Fragments arranged by size:
2995 1730 1018
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3098 844
Size: 2254 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 2181 3630 1394
Size: 429 358 1449 3507
Fragments arranged by size:
3507 1449 429 358
BsrDI GCAATG_nn'
Cuts at: 65 1248 4238 4412 65
Size: 1183 2990 174 1396
Fragments arranged by size:
2990 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4387 4574 1700
Size: 110 2577 187 2869
Fragments arranged by size:
2869 2577 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5743
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5743
BssSI C'ACGA_G
Cuts at: 2036 3803 4815 2036
Size: 1767 1012 2964
Fragments arranged by size:
2964 1767 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5743
BstBI TT'CG_AA
Cuts at: 4547 4547
Size: 5743
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5743
BstXI CCAn_nnnn'nTGG
Cuts at: 2278 2505 2278
Size: 227 5516
BstYI r'GATC_y
Cuts at: 1972 2314 2524 2845 3073 3440 3825 3842 5294
Size: 342 210 321 228 367 385 17 1452
Cuts at: 5294 5380 5392 5738 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1452 385 367 346 342 321 228
210 86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4252
Fragments arranged by size:
4252 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5004
Fragments arranged by size:
5004 683 56
BtsI GCAGTG_nn'
Cuts at: 2178 2213 3259 4053 4073 2178
Size: 35 1046 794 20 3848
Fragments arranged by size:
3848 1046 794 35 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5743
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2389 2424 529
Size: 35 1825 35 3848
Fragments arranged by size:
3848 1825 35 35
DraI TTT'AAA
Cuts at: 3047 3047
Size: 5743
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4750 809
Size: 507 3434 1802
Fragments arranged by size:
3434 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2701 3190 4079
Size: 54 1425 50 275 18 871 489 889
Cuts at: 4079 5708 8
Size: 1629 43
Fragments arranged by size:
1629 1425 889 871 489 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3190 1537
Size: 275 1378 4090
Fragments arranged by size:
4090 1378 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2469 2830 3673 1353
Size: 1116 361 843 3423
Fragments arranged by size:
3423 1116 843 361
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4330 4704 4850 387
Size: 1064 138 2741 374 146 1280
Fragments arranged by size:
2741 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2059 2172 2748 2754 3806 4403 5190
Size: 393 427 113 576 6 1052 597 787
Cuts at: 5190 5458 1239
Size: 268 1524
Fragments arranged by size:
1524 1052 787 597 576 427 393 268
113 6
EcoO109I rG'GnC_Cy
Cuts at: 2059 2059
Size: 5743
EcoRI G'AATT_C
Cuts at: 2900 3052 3430 2900
Size: 152 378 5213
Fragments arranged by size:
5213 378 152
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2038 2038
Size: 5743
FspI TGC'GCA
Cuts at: 4249 4249
Size: 5743
HaeII r_GCGC'y
Cuts at: 1678 4890 1678
Size: 3212 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 4384 4416 4458 4490 1373
Size: 32 135 32 2812 32 42 32 2626
Fragments arranged by size:
2812 2626 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2098 3087 3333 669
Size: 988 281 160 989 246 3079
Fragments arranged by size:
3079 989 988 281 246 160
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5743
HpaI GTT'AAC
Cuts at: 3333 3333
Size: 5743
KpnI G_GTAC'C
Cuts at: 3068 3068
Size: 5743
MfeI C'AATT_G
Cuts at: 3342 3342
Size: 5743
MluI A'CGCG_T
Cuts at: 5619 5619
Size: 5743
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2873 4857 5041 1305
Size: 1568 1984 184 2007
Fragments arranged by size:
2007 1984 1568 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3921
Fragments arranged by size:
3921 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2276 2291 2703 3701 4060 4219 518
Size: 648 1110 15 412 998 359 159 2042
Fragments arranged by size:
2042 1110 998 648 412 359 159 15
MspA1I CmG'CkG
Cuts at: 1606 3159 3836 4984 5229 1606
Size: 1553 677 1148 245 2120
Fragments arranged by size:
2120 1553 1148 677 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4252
Fragments arranged by size:
4252 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5743
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5743
NotI GC'GGCC_GC
Cuts at: 3190 3190
Size: 5743
NspI r_CATG'y
Cuts at: 1132 1229 3101 4646 1132
Size: 97 1872 1545 2229
Fragments arranged by size:
2229 1872 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5743
PciI A'CATG_T
Cuts at: 4642 4642
Size: 5743
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2726 1108
Size: 1618 4125
PmeI GTTT'AAAC
Cuts at: 3047 3047
Size: 5743
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3812 3844 5325 5357 3812
Size: 32 1481 32 4198
Fragments arranged by size:
4198 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5743
PsiI TTA'TAA
Cuts at: 2997 3313 2997
Size: 316 5427
PstI C_TGCA'G
Cuts at: 830 2758 3095 830
Size: 1928 337 3478
Fragments arranged by size:
3478 1928 337
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5743
SalI G'TCGA_C
Cuts at: 1936 3085 1936
Size: 1149 4594
SapI GCTCTTCn'nnn_
Cuts at: 2469 2469
Size: 5743
SbfI CC_TGCA'GG
Cuts at: 3095 3095
Size: 5743
ScaI AGT'ACT
Cuts at: 2358 3991 5703 2358
Size: 1633 1712 2398
Fragments arranged by size:
2398 1712 1633
SexAI A'CCwGG_T
Cuts at: 2557 2557
Size: 5743
SfcI C'TryA_G
Cuts at: 826 1045 2220 2754 2820 3091 4226 4907 5098
Size: 219 1175 534 66 271 1135 681 191
Cuts at: 5098 826
Size: 1471
Fragments arranged by size:
1471 1175 1135 681 534 271 219 191
66
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5743
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5743
SmaI CCC'GGG
Cuts at: 1872 3070 1872
Size: 1198 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2457 3847 4748 5010 820
Size: 197 926 514 1390 901 262 1553
Fragments arranged by size:
1553 1390 926 901 514 262 197
SnaBI TAC'GTA
Cuts at: 493 3534 493
Size: 3041 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5743
SphI G_CATG'C
Cuts at: 1132 3101 1132
Size: 1969 3774
SspI AAT'ATT
Cuts at: 5 52 3667 5
Size: 47 3615 2081
Fragments arranged by size:
3615 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5743
StyI C'CwwG_G
Cuts at: 513 1065 2004 3182 513
Size: 552 939 1178 3074
Fragments arranged by size:
3074 1178 939 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3770 3787 3940 4125 4570 325
Size: 1255 2190 17 153 185 445 1498
Fragments arranged by size:
2190 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2356 3989 5701
Size: 274 80 33 51 1443 379 1633 1712
Cuts at: 5701 96
Size: 138
Fragments arranged by size:
1712 1633 1443 379 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5703 5735 5703
Size: 32 5711
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5743
XbaI T'CTAG_A
Cuts at: 3079 3079
Size: 5743
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5743
XmaI C'CCGG_G
Cuts at: 1870 3068 1870
Size: 1198 4545
XmnI GAAnn'nnTTC
Cuts at: 3872 3872
Size: 5743
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3551 276
Size: 53 83 186 936 2017 2468
Fragments arranged by size:
2468 2017 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BcgI BciVI BclI
BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI
BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI
CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI
EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII
HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I
NcoI NdeI NheI NotI NspI PasI PciI PflMI
PmeI PpiI PshAI PsiI PstI PvuI SalI SapI
SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstZ17I Bsu36I
DraIII EcoICRI EcoNI EcoRV FseI FspAI KasI NaeI
NarI NgoMIV NruI NsiI PacI PfoI PmlI PpuMI
PspOMI PspXI PsrI PvuII RsrII SacI SacII SanDI
SfiI SfoI SrfI SwaI XcmI