Restriction map for
pFN21AB5542
(Circular) (Six-base) MAPSORT of: pFN21AB5542.seq Check: 6476 from: 1 to: 5779
pFN21AB5542 5779 bp
With 182 enzymes: SgfI *
January 16, 2009 15:25 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3589 278
Size: 53 83 186 936 2053 2468
Fragments arranged by size:
2468 2053 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3122 1937
Size: 1185 4594
Acc65I G'GTAC_C
Cuts at: 3100 3100
Size: 5779
AclI AA'CG_TT
Cuts at: 1563 3906 4279 1563
Size: 2343 373 3063
Fragments arranged by size:
3063 2343 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3842 5226 5494 1632
Size: 2210 1384 268 1917
Fragments arranged by size:
2210 1917 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5582
AflIII A'CryG_T
Cuts at: 1203 2629 2838 4678 5655 1203
Size: 1426 209 1840 977 1327
Fragments arranged by size:
1840 1426 1327 977 209
AgeI A'CCGG_T
Cuts at: 4610 4610
Size: 5779
AhdI GACnn_n'nnGTC
Cuts at: 4508 4508
Size: 5779
AleI CACnn'nnGTG
Cuts at: 1166 2099 2558 1166
Size: 933 459 4387
Fragments arranged by size:
4387 933 459
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5779
AlwNI CAG_nnn'CTG
Cuts at: 3522 5094 3522
Size: 1572 4207
ApaI G_GGCC'C
Cuts at: 2382 2683 2382
Size: 301 5478
ApaLI G'TGCA_C
Cuts at: 3836 4992 3836
Size: 1156 4623
ApoI r'AATT_y
Cuts at: 1493 3088 3314 3466 5575 1493
Size: 1595 226 152 2109 1697
Fragments arranged by size:
2109 1697 1595 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4333 160
Size: 872 3301 1606
Fragments arranged by size:
3301 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5779
AvaI C'yCGr_G
Cuts at: 1870 1943 2867 3104 1870
Size: 73 924 237 4545
Fragments arranged by size:
4545 924 237 73
BamHI G'GATC_C
Cuts at: 3109 3476 3109
Size: 367 5412
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2203 2212 2812 3100 4555
Size: 325 217 627 416 9 600 288 1455
Cuts at: 4555 618
Size: 1842
Fragments arranged by size:
1842 1455 627 600 416 325 288 217
9
BanII G_rGCy'C
Cuts at: 1391 2072 2127 2382 2619 2683 2757 2874 3005
Size: 681 55 255 237 64 74 117 131
Cuts at: 3005 3098 1391
Size: 93 4072
Fragments arranged by size:
4072 681 255 237 131 117 93 74
64 55
BbeI G_GCGC'C
Cuts at: 2216 2216
Size: 5779
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2556 928
Size: 971 657 4151
Fragments arranged by size:
4151 971 657
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2185 2219 3970 4004 1903
Size: 34 248 34 1751 34 3678
Fragments arranged by size:
3678 1751 248 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3671 4881 3671
Size: 1210 4569
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5779
BfrBI ATG'CAT
Cuts at: 2362 2362
Size: 5779
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2386 2522 4390
Size: 107 122 71 631 605 714 136 1868
Cuts at: 4390 136
Size: 1525
Fragments arranged by size:
1868 1525 714 631 605 136 122 107
71
BglII A'GATC_T
Cuts at: 2490 5774 2490
Size: 3284 2495
BlpI GC'TnA_GC
Cuts at: 3196 3196
Size: 5779
Bme1580I G_kGCm'C
Cuts at: 1790 2382 2683 3055 3840 4996 1790
Size: 592 301 372 785 1156 2573
Fragments arranged by size:
2573 1156 785 592 372 301
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5779
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2155 3510 4468
Size: 416 53 83 399 24 738 1355 958
Cuts at: 4468 442
Size: 1753
Fragments arranged by size:
1753 1355 958 738 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5779
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2603 2633 2841 2985 4439 1239
Size: 1364 30 208 144 1454 2579
Fragments arranged by size:
2579 1454 1364 208 144 30
Bpu10I CC'TnA_GC
Cuts at: 3004 3004
Size: 5779
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3904 4769 5067 3904
Size: 865 298 4616
Fragments arranged by size:
4616 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2346 2756 2965 4442 882
Size: 617 847 410 209 1477 2219
Fragments arranged by size:
2219 1477 847 617 410 209
BsaAI yAC'GTr
Cuts at: 493 1204 2149 2632 2841 3570 493
Size: 711 945 483 209 729 2702
Fragments arranged by size:
2702 945 729 711 483 209
BsaBI GATnn'nnATC
Cuts at: 5773 5773
Size: 5779
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2184 2213 3586 3968
Size: 53 83 186 936 651 29 1373 382
Cuts at: 3968 4603 275
Size: 635 1451
Fragments arranged by size:
1451 1373 936 651 635 382 186 83
53 29
BsaWI w'CCGG_w
Cuts at: 4212 4610 4884 5031 5442 4212
Size: 398 274 147 411 4549
Fragments arranged by size:
4549 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2444 2474 2958 2988 3034
Size: 30 165 30 1041 30 484 30 46
Cuts at: 3034 3064 1178
Size: 30 3893
Fragments arranged by size:
3893 1041 484 165 46 30 30 30
30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2021 2840 1186
Size: 835 819 4125
Fragments arranged by size:
4125 835 819
BseYI C'CCAG_C
Cuts at: 2374 2676 2901 4982 5478 2374
Size: 302 225 2081 496 2675
Fragments arranged by size:
2675 2081 496 302 225
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2870 1163
Size: 573 1134 4072
Fragments arranged by size:
4072 1134 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2116 3229 3990 5018 1540
Size: 275 186 115 1113 761 1028 2301
Fragments arranged by size:
2301 1113 1028 761 275 186 115
BsiHKAI G_wGCw'C
Cuts at: 2532 2757 3005 3840 3925 4996 2532
Size: 225 248 835 85 1071 3315
Fragments arranged by size:
3315 1071 835 248 225 85
BsmI GAATG_Cn'
Cuts at: 3290 3383 3290
Size: 93 5686
BsmBI CGTCTCn'nnnn_
Cuts at: 2591 2591
Size: 5779
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2072 2127 2382 2532 2619 2683 2757
Size: 399 282 55 255 150 87 64 74
Cuts at: 2757 2874 3005 3055 3098 3840 3925 4996 1391
Size: 117 131 50 43 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 399 282 255 150 131
117 87 85 74 64 55 50 43
BspEI T'CCGG_A
Cuts at: 5442 5442
Size: 5779
BspHI T'CATG_A
Cuts at: 3668 5398 3668
Size: 1730 4049
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2307 2463 3134 844
Size: 1463 156 671 3489
Fragments arranged by size:
3489 1463 671 156
BsrBI CCG'CTC
Cuts at: 1394 1823 3666 1394
Size: 429 1843 3507
Fragments arranged by size:
3507 1843 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2928 4274 4448 65
Size: 1183 1680 1346 174 1396
Fragments arranged by size:
1680 1396 1346 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2893 4423 4610 1700
Size: 110 1083 1530 187 2869
Fragments arranged by size:
2869 1530 1083 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5779
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5779
BssSI C'ACGA_G
Cuts at: 2756 3839 4851 2756
Size: 1083 1012 3684
Fragments arranged by size:
3684 1083 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2359 794
Size: 1565 4214
BstBI TT'CG_AA
Cuts at: 4583 4583
Size: 5779
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5779
BstYI r'GATC_y
Cuts at: 1972 2490 2951 3109 3476 3861 3878 5330 5416
Size: 518 461 158 367 385 17 1452 86
Cuts at: 5416 5428 5774 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 518 461 385 367 346 158
86 17 12
Bsu36I CC'TnA_GG
Cuts at: 2669 2669
Size: 5779
BtgI C'CryG_G
Cuts at: 513 1065 2004 2116 2207 2216 2398 2469 513
Size: 552 939 112 91 9 182 71 3823
Fragments arranged by size:
3823 939 552 182 112 91 71 9
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5040
Fragments arranged by size:
5040 683 56
BtsI GCAGTG_nn'
Cuts at: 2360 3295 4089 4109 2360
Size: 935 794 20 4030
Fragments arranged by size:
4030 935 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5779
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5744
DraI TTT'AAA
Cuts at: 3083 3083
Size: 5779
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4786 809
Size: 507 3470 1802
Fragments arranged by size:
3470 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2113 3226 4115
Size: 54 1425 50 275 18 283 1113 889
Cuts at: 4115 5744 8
Size: 1629 43
Fragments arranged by size:
1629 1425 1113 889 283 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2113 3226 1537
Size: 275 301 1113 4090
Fragments arranged by size:
4090 1113 301 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2993 3709 1353
Size: 1640 716 3423
Fragments arranged by size:
3423 1640 716
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2540 4366 4740 4886 387
Size: 1064 138 951 1826 374 146 1280
Fragments arranged by size:
1826 1280 1064 951 374 146 138
EcoICRI GAG'CTC
Cuts at: 2755 3003 2755
Size: 248 5531
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2603 2633 2841 2985 3842 4439 5226
Size: 393 971 30 208 144 857 597 787
Cuts at: 5226 5494 1239
Size: 268 1524
Fragments arranged by size:
1524 971 857 787 597 393 268 208
144 30
EcoNI CCTnn'n_nnAGG
Cuts at: 2036 2447 2585 2036
Size: 411 138 5230
Fragments arranged by size:
5230 411 138
EcoO109I rG'GnC_Cy
Cuts at: 2041 2378 2452 2680 2041
Size: 337 74 228 5140
Fragments arranged by size:
5140 337 228 74
EcoRI G'AATT_C
Cuts at: 3088 3466 3088
Size: 378 5401
FspI TGC'GCA
Cuts at: 4285 4285
Size: 5779
HaeII r_GCGC'y
Cuts at: 1678 2216 2268 2392 4926 1678
Size: 538 52 124 2534 2531
Fragments arranged by size:
2534 2531 538 124 52
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2324 2356 2444 2476 2489
Size: 32 135 32 752 32 88 32 13
Cuts at: 2489 2521 2943 2975 4420 4452 4494 4526 1373
Size: 32 422 32 1445 32 42 32 2626
Fragments arranged by size:
2626 1445 752 422 135 88 42 32
32 32 32 32 32 32 32 13
HincII GTy'rAC
Cuts at: 669 1657 1938 3123 3369 669
Size: 988 281 1185 246 3079
Fragments arranged by size:
3079 1185 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5779
HpaI GTT'AAC
Cuts at: 3369 3369
Size: 5779
KasI G'GCGC_C
Cuts at: 2212 2212
Size: 5779
KpnI G_GTAC'C
Cuts at: 3104 3104
Size: 5779
MfeI C'AATT_G
Cuts at: 3378 3378
Size: 5779
MluI A'CGCG_T
Cuts at: 5655 5655
Size: 5779
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4893 5077 1305
Size: 3588 184 2007
Fragments arranged by size:
3588 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3957
Fragments arranged by size:
3957 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2099 2138 2558 3737 4096 4255 518
Size: 648 933 39 420 1179 359 159 2042
Fragments arranged by size:
2042 1179 933 648 420 359 159 39
MspA1I CmG'CkG
Cuts at: 1606 2118 2209 2400 2471 2804 3195 3872 5020
Size: 512 91 191 71 333 391 677 1148
Cuts at: 5020 5265 1606
Size: 245 2120
Fragments arranged by size:
2120 1148 677 512 391 333 245 191
91 71
NaeI GCC'GGC
Cuts at: 2895 2895
Size: 5779
NarI GG'CG_CC
Cuts at: 2213 2213
Size: 5779
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4288
Fragments arranged by size:
4288 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5779
NgoMIV G'CCGG_C
Cuts at: 2893 2893
Size: 5779
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5779
NotI GC'GGCC_GC
Cuts at: 3226 3226
Size: 5779
NsiI A_TGCA'T
Cuts at: 2364 2364
Size: 5779
NspI r_CATG'y
Cuts at: 1132 1229 2559 3137 4682 1132
Size: 97 1330 578 1545 2229
Fragments arranged by size:
2229 1545 1330 578 97
PasI CC'CwG_GG
Cuts at: 1348 2683 3055 1348
Size: 1335 372 4072
Fragments arranged by size:
4072 1335 372
PciI A'CATG_T
Cuts at: 4678 4678
Size: 5779
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5779
PfoI T'CCnGG_A
Cuts at: 2462 2462
Size: 5779
PmeI GTTT'AAAC
Cuts at: 3083 3083
Size: 5779
PmlI CAC'GTG
Cuts at: 2632 2841 2632
Size: 209 5570
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3848 3880 5361 5393 3848
Size: 32 1481 32 4234
Fragments arranged by size:
4234 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2452 2452
Size: 5779
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5779
PsiI TTA'TAA
Cuts at: 3349 3349
Size: 5779
PspOMI G'GGCC_C
Cuts at: 2378 2679 2378
Size: 301 5478
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 2396 2428 2396
Size: 32 5747
PstI C_TGCA'G
Cuts at: 830 2355 2776 3131 830
Size: 1525 421 355 3478
Fragments arranged by size:
3478 1525 421 355
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5779
PvuII CAG'CTG
Cuts at: 2804 2804
Size: 5779
SacI G_AGCT'C
Cuts at: 2757 3005 2757
Size: 248 5531
SacII CC_GC'GG
Cuts at: 2119 2210 2401 2472 2119
Size: 91 191 71 5426
Fragments arranged by size:
5426 191 91 71
SalI G'TCGA_C
Cuts at: 1936 3121 1936
Size: 1185 4594
SapI GCTCTTCn'nnn_
Cuts at: 2993 2993
Size: 5779
SbfI CC_TGCA'GG
Cuts at: 2776 3131 2776
Size: 355 5424
ScaI AGT'ACT
Cuts at: 4027 5739 4027
Size: 1712 4067
SfcI C'TryA_G
Cuts at: 826 1045 2351 2772 3127 4262 4943 5134 826
Size: 219 1306 421 355 1135 681 191 1471
Fragments arranged by size:
1471 1306 1135 681 421 355 219 191
SfoI GGC'GCC
Cuts at: 2214 2214
Size: 5779
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5779
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5779
SmaI CCC'GGG
Cuts at: 1872 3106 1872
Size: 1234 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3883 4784 5046 820
Size: 197 926 1940 901 262 1553
Fragments arranged by size:
1940 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3570 493
Size: 3077 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5779
SphI G_CATG'C
Cuts at: 1132 3137 1132
Size: 2005 3774
SspI AAT'ATT
Cuts at: 5 52 3703 5
Size: 47 3651 2081
Fragments arranged by size:
3651 2081 47
StuI AGG'CCT
Cuts at: 1517 2770 1517
Size: 1253 4526
StyI C'CwwG_G
Cuts at: 513 1065 2004 2947 3218 513
Size: 552 939 943 271 3074
Fragments arranged by size:
3074 943 939 552 271
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2174 3806 3823 3976 4161 4606 325
Size: 1255 594 1632 17 153 185 445 1498
Fragments arranged by size:
1632 1498 1255 594 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4025 5737 96
Size: 274 80 33 51 1443 2048 1712 138
Fragments arranged by size:
2048 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2271 2303 3034 3066 5739 5771 2271
Size: 32 731 32 2673 32 2279
Fragments arranged by size:
2673 2279 731 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5779
XbaI T'CTAG_A
Cuts at: 3115 3115
Size: 5779
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2917 2917
Size: 5779
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5779
XmaI C'CCGG_G
Cuts at: 1870 3104 1870
Size: 1234 4545
XmnI GAAnn'nnTTC
Cuts at: 2087 3908 2087
Size: 1821 3958
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3587 276
Size: 53 83 186 936 2053 2468
Fragments arranged by size:
2468 2053 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI
BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI
Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I
EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI
KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI
NarI NcoI NdeI NgoMIV NheI NotI NsiI NspI
PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI
PshAI PsiI PspOMI PsrI PstI PvuI PvuII SacI
SacII SalI SapI SbfI ScaI SfcI SfoI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI AscI AvrII BaeI BbvCI BplI
BsiWI BstXI BstZ17I DraIII EcoRV FalI FseI FspAI
NruI PacI PspXI RsrII SanDI SexAI SfiI SrfI
SwaI