Restriction map for
pFN21AB5541
(Circular) (Six-base) MAPSORT of: pFN21AB5541.seq Check: 1205 from: 1 to: 7636
pFN21AB5541 7636 bp
With 182 enzymes: SgfI *
February 27, 2009 14:34 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 2652 3024 4413 5446
Size: 53 83 186 936 1116 372 1389 1033
Cuts at: 5446 278
Size: 2468
Fragments arranged by size:
2468 1389 1116 1033 936 372 186 83
53
AccI GT'mk_AC
Cuts at: 1937 2252 3119 4979 1937
Size: 315 867 1860 4594
Fragments arranged by size:
4594 1860 867 315
Acc65I G'GTAC_C
Cuts at: 4957 4957
Size: 7636
AclI AA'CG_TT
Cuts at: 1563 5763 6136 1563
Size: 4200 373 3063
Fragments arranged by size:
4200 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3219 5699 7083 7351 1632
Size: 1587 2480 1384 268 1917
Fragments arranged by size:
2480 1917 1587 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 3326 820
Size: 197 2309 5130
Fragments arranged by size:
5130 2309 197
AflIII A'CryG_T
Cuts at: 1203 2203 3540 6535 7512 1203
Size: 1000 1337 2995 977 1327
Fragments arranged by size:
2995 1337 1327 1000 977
AgeI A'CCGG_T
Cuts at: 6467 6467
Size: 7636
AhdI GACnn_n'nnGTC
Cuts at: 6365 6365
Size: 7636
AleI CACnn'nnGTG
Cuts at: 1166 3758 1166
Size: 2592 5044
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2344 3377 4355 1184
Size: 1160 1033 978 4465
Fragments arranged by size:
4465 1160 1033 978
AlwNI CAG_nnn'CTG
Cuts at: 3469 3490 5379 6951 3469
Size: 21 1889 1572 4154
Fragments arranged by size:
4154 1889 1572 21
ApaI G_GGCC'C
Cuts at: 2961 2961
Size: 7636
ApaLI G'TGCA_C
Cuts at: 5693 6849 5693
Size: 1156 6480
ApoI r'AATT_y
Cuts at: 1493 3708 4667 4945 5171 5323 7432 1493
Size: 2215 959 278 226 152 2109 1697
Fragments arranged by size:
2215 2109 1697 959 278 226 152
AseI AT'TA_AT
Cuts at: 160 1032 6190 160
Size: 872 5158 1606
Fragments arranged by size:
5158 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7636
AvaI C'yCGr_G
Cuts at: 1870 1943 2054 2514 3261 3302 3565 3812 4024
Size: 73 111 460 747 41 263 247 212
Cuts at: 4024 4961 1870
Size: 937 4545
Fragments arranged by size:
4545 937 747 460 263 247 212 111
73 41
BamHI G'GATC_C
Cuts at: 2658 3804 4450 4966 5333 2658
Size: 1146 646 516 367 4961
Fragments arranged by size:
4961 1146 646 516 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3569 4195 4367 4957 6412
Size: 325 217 627 1782 626 172 590 1455
Cuts at: 6412 618
Size: 1842
Fragments arranged by size:
1842 1782 1455 627 626 590 325 217
172
BanII G_rGCy'C
Cuts at: 1391 2523 2961 3534 3566 3999 4899 4908 4955
Size: 1132 438 573 32 433 900 9 47
Cuts at: 4955 1391
Size: 4072
Fragments arranged by size:
4072 1132 900 573 438 433 47 32
9
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 4245 4413 928
Size: 971 2346 168 4151
Fragments arranged by size:
4151 2346 971 168
BbvCI CC'TCA_GC
Cuts at: 3747 4468 3747
Size: 721 6915
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 2897 2931 3920 3954 4462
Size: 34 50 34 876 34 989 34 508
Cuts at: 4462 4496 5827 5861 1903
Size: 34 1331 34 3678
Fragments arranged by size:
3678 1331 989 876 508 50 34 34
34 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 5528 6738 5528
Size: 1210 6426
BclI T'GATC_A
Cuts at: 1548 3003 1548
Size: 1455 6181
BfrBI ATG'CAT
Cuts at: 3409 3409
Size: 7636
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2009 2014 3638
Size: 107 122 71 631 605 337 5 1624
Cuts at: 3638 6247 136
Size: 2609 1525
Fragments arranged by size:
2609 1624 1525 631 605 337 122 107
71 5
BglII A'GATC_T
Cuts at: 4699 7631 4699
Size: 2932 4704
BlpI GC'TnA_GC
Cuts at: 4232 5053 4232
Size: 821 6815
Bme1580I G_kGCm'C
Cuts at: 1790 2928 2961 3572 3755 5697 6853 1790
Size: 1138 33 611 183 1942 1156 2573
Fragments arranged by size:
2573 1942 1156 1138 611 183 33
BmgBI CAC'GTC
Cuts at: 1318 2575 3543 3588 3790 4537 4806 1318
Size: 1257 968 45 202 747 269 4148
Fragments arranged by size:
4148 1257 968 747 269 202 45
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 4910 5367 6325
Size: 416 53 83 399 24 3493 457 958
Cuts at: 6325 442
Size: 1753
Fragments arranged by size:
3493 1753 958 457 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 7636
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4721 4721
Size: 7636
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 3152 3410 3473 3528 4013 4206 4505 4522
Size: 1913 258 63 55 485 193 299 17
Cuts at: 4522 4634 4908 6296 1239
Size: 112 274 1388 2579
Fragments arranged by size:
2579 1913 1388 485 299 274 258 193
112 63 55 17
Bpu10I CC'TnA_GC
Cuts at: 3350 3401 3519 3747 4468 3350
Size: 51 118 228 721 6518
Fragments arranged by size:
6518 721 228 118 51
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3088 5761 6626 6924 3088
Size: 2673 865 298 3800
Fragments arranged by size:
3800 2673 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2741 4059 6299 882
Size: 617 1242 1318 2240 2219
Fragments arranged by size:
2240 2219 1318 1242 617
BsaAI yAC'GTr
Cuts at: 493 1204 4303 4421 4480 5427 493
Size: 711 3099 118 59 947 2702
Fragments arranged by size:
3099 2702 947 711 118 59
BsaBI GATnn'nnATC
Cuts at: 2729 7630 2729
Size: 4901 2735
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2649 2846 2973 3021
Size: 53 83 186 936 1116 197 127 48
Cuts at: 3021 4410 4929 5443 5825 6460 275
Size: 1389 519 514 382 635 1451
Fragments arranged by size:
1451 1389 1116 936 635 519 514 382
197 186 127 83 53 48
BsaWI w'CCGG_w
Cuts at: 2399 2523 6069 6467 6741 6888 7299 2399
Size: 124 3546 398 274 147 411 2736
Fragments arranged by size:
3546 2736 411 398 274 147 124
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2657 2687 4028 4058 1178
Size: 30 165 30 1254 30 1341 30 4756
Fragments arranged by size:
4756 1341 1254 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 3287 4694 4814 1186
Size: 2101 1407 120 4008
Fragments arranged by size:
4008 2101 1407 120
BseYI C'CCAG_C
Cuts at: 3011 3372 3581 4768 4908 6839 7335 3011
Size: 361 209 1187 140 1931 496 3312
Fragments arranged by size:
3312 1931 1187 496 361 209 140
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2336 3877 3960 4876 1163
Size: 573 600 1541 83 916 3923
Fragments arranged by size:
3923 1541 916 600 573 83
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4578 5086 5847 6875 1540
Size: 275 186 2577 508 761 1028 2301
Fragments arranged by size:
2577 2301 1028 761 508 275 186
BsiHKAI G_wGCw'C
Cuts at: 2523 3356 3534 3566 3870 4485 5697 5782 6853
Size: 833 178 32 304 615 1212 85 1071
Cuts at: 6853 2523
Size: 3306
Fragments arranged by size:
3306 1212 1071 833 615 304 178 85
32
BsmI GAATG_Cn'
Cuts at: 2536 5147 5240 2536
Size: 2611 93 4932
Fragments arranged by size:
4932 2611 93
BsmBI CGTCTCn'nnnn_
Cuts at: 4757 4812 4757
Size: 55 7581
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2523 2928 2961 3356 3534 3566 3572
Size: 399 733 405 33 395 178 32 6
Cuts at: 3572 3755 3870 3999 4485 4899 4908 4955 5697
Size: 183 115 129 486 414 9 47 742
Cuts at: 5697 5782 6853 1391
Size: 85 1071 2174
Fragments arranged by size:
2174 1071 742 733 486 414 405 399
395 183 178 129 115 85 47 33
32 9 6
BspEI T'CCGG_A
Cuts at: 7299 7299
Size: 7636
BspHI T'CATG_A
Cuts at: 5525 7255 5525
Size: 1730 5906
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2182 4668 4991 844
Size: 1338 2486 323 3489
Fragments arranged by size:
3489 2486 1338 323
BsrBI CCG'CTC
Cuts at: 1394 1823 5523 1394
Size: 429 3700 3507
Fragments arranged by size:
3700 3507 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3921 4413 6131 6305 65
Size: 1183 2673 492 1718 174 1396
Fragments arranged by size:
2673 1718 1396 1183 492 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2008 3906 6280 6467 1700
Size: 110 198 1898 2374 187 2869
Fragments arranged by size:
2869 2374 1898 198 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 7636
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 7636
BssSI C'ACGA_G
Cuts at: 2906 4862 4892 5696 6708 2906
Size: 1956 30 804 1012 3834
Fragments arranged by size:
3834 1956 1012 804 30
BstAPI GCAn_nnn'nTGC
Cuts at: 794 3490 794
Size: 2696 4940
BstBI TT'CG_AA
Cuts at: 6440 6440
Size: 7636
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 7636
BstXI CCAn_nnnn'nTGG
Cuts at: 2969 3130 3506 4328 2969
Size: 161 376 822 6277
Fragments arranged by size:
6277 822 376 161
BstYI r'GATC_y
Cuts at: 1972 2179 2230 2658 3459 3804 4450 4699 4966
Size: 207 51 428 801 345 646 249 267
Cuts at: 4966 5333 5718 5735 7187 7273 7285 7631 1972
Size: 367 385 17 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 801 646 428 385 367 346
345 267 249 207 86 51 17 12
BstZ17I GTA'TAC
Cuts at: 2253 2253
Size: 7636
Bsu36I CC'TnA_GG
Cuts at: 2565 2565
Size: 7636
BtgI C'CryG_G
Cuts at: 513 1065 2929 3312 3739 4608 4845 513
Size: 552 1864 383 427 869 237 3304
Fragments arranged by size:
3304 1864 869 552 427 383 237
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 4460 487
Size: 683 56 3234 3663
Fragments arranged by size:
3663 3234 683 56
BtsI GCAGTG_nn'
Cuts at: 2558 4167 5152 5946 5966 2558
Size: 1609 985 794 20 4228
Fragments arranged by size:
4228 1609 985 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 7636
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2187 2222 2524 2559 529
Size: 35 1623 35 302 35 5606
Fragments arranged by size:
5606 1623 302 35 35 35
DraI TTT'AAA
Cuts at: 2172 4940 2172
Size: 2768 4868
DraIII CAC_nnn'GTG
Cuts at: 2599 2799 2599
Size: 200 7436
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2770 3839 4771 6643 809
Size: 507 1454 1069 932 1872 1802
Fragments arranged by size:
1872 1802 1454 1069 932 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3792 5083 5972
Size: 54 1425 50 275 18 1962 1291 889
Cuts at: 5972 7601 8
Size: 1629 43
Fragments arranged by size:
1962 1629 1425 1291 889 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 5083 1537
Size: 275 3271 4090
Fragments arranged by size:
4090 3271 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2205 2466 3794 4877 5566 1353
Size: 852 261 1328 1083 689 3423
Fragments arranged by size:
3423 1328 1083 852 689 261
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 6223 6597 6743 387
Size: 1064 138 4634 374 146 1280
Fragments arranged by size:
4634 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2521 3532 3564 2521
Size: 1011 32 6593
Fragments arranged by size:
6593 1011 32
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 3152 3219 3410 3473 3528 4013 4206
Size: 393 1520 67 191 63 55 485 193
Cuts at: 4206 4505 4522 4634 4908 5699 6296 7083 7351
Size: 299 17 112 274 791 597 787 268
Cuts at: 7351 1239
Size: 1524
Fragments arranged by size:
1524 1520 791 787 597 485 393 299
274 268 193 191 112 67 63 55
17
EcoO109I rG'GnC_Cy
Cuts at: 2194 2957 2958 3307 3597 4674 4773 2194
Size: 763 1 349 290 1077 99 5057
Fragments arranged by size:
5057 1077 763 349 290 99 1
EcoRI G'AATT_C
Cuts at: 4945 5323 4945
Size: 378 7258
FspI TGC'GCA
Cuts at: 3981 6142 3981
Size: 2161 5475
HaeII r_GCGC'y
Cuts at: 1678 6783 1678
Size: 5105 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2624 2656 2836 2868 3394
Size: 32 135 32 1052 32 180 32 526
Cuts at: 3394 3426 4438 4470 6277 6309 6351 6383 1373
Size: 32 1012 32 1807 32 42 32 2626
Fragments arranged by size:
2626 1807 1052 1012 526 180 135 42
32 32 32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2112 3226 4980 5226 669
Size: 988 281 174 1114 1754 246 3079
Fragments arranged by size:
3079 1754 1114 988 281 246 174
HindIII A'AGCT_T
Cuts at: 748 748
Size: 7636
HpaI GTT'AAC
Cuts at: 5226 5226
Size: 7636
KpnI G_GTAC'C
Cuts at: 4961 4961
Size: 7636
MfeI C'AATT_G
Cuts at: 3756 5235 3756
Size: 1479 6157
MluI A'CGCG_T
Cuts at: 7512 7512
Size: 7636
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3627 4158 6750 6934 1305
Size: 2322 531 2592 184 2007
Fragments arranged by size:
2592 2322 2007 531 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 5814
Fragments arranged by size:
5814 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2326 2495 2733 3758 4094 5594 5953
Size: 648 1160 169 238 1025 336 1500 359
Cuts at: 5953 6112 518
Size: 159 2042
Fragments arranged by size:
2042 1500 1160 1025 648 359 336 238
169 159
MspA1I CmG'CkG
Cuts at: 1606 2704 2931 3490 3847 4345 5052 5729 6877
Size: 1098 227 559 357 498 707 677 1148
Cuts at: 6877 7122 1606
Size: 245 2120
Fragments arranged by size:
2120 1148 1098 707 677 559 498 357
245 227
NaeI GCC'GGC
Cuts at: 2010 2010
Size: 7636
NcoI C'CATG_G
Cuts at: 513 1065 3739 4845 513
Size: 552 2674 1106 3304
Fragments arranged by size:
3304 2674 1106 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 7636
NgoMIV G'CCGG_C
Cuts at: 2008 2008
Size: 7636
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 7636
NotI GC'GGCC_GC
Cuts at: 5083 5083
Size: 7636
NsiI A_TGCA'T
Cuts at: 3411 3411
Size: 7636
NspI r_CATG'y
Cuts at: 1132 1229 2135 2207 3409 3775 4994 6539 1132
Size: 97 906 72 1202 366 1219 1545 2229
Fragments arranged by size:
2229 1545 1219 1202 906 366 97 72
PasI CC'CwG_GG
Cuts at: 1348 3594 1348
Size: 2246 5390
PciI A'CATG_T
Cuts at: 2203 6535 2203
Size: 4332 3304
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 7636
PfoI T'CCnGG_A
Cuts at: 3034 3534 4530 3034
Size: 500 996 6140
Fragments arranged by size:
6140 996 500
PmeI GTTT'AAAC
Cuts at: 4940 4940
Size: 7636
PmlI CAC'GTG
Cuts at: 4421 4480 4421
Size: 59 7577
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5705 5737 7218 7250 5705
Size: 32 1481 32 6091
Fragments arranged by size:
6091 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2194 4674 4773 2194
Size: 2480 99 5057
Fragments arranged by size:
5057 2480 99
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 7636
PsiI TTA'TAA
Cuts at: 5206 5206
Size: 7636
PspOMI G'GGCC_C
Cuts at: 2957 2957
Size: 7636
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 4244 4276 4244
Size: 32 7604
PstI C_TGCA'G
Cuts at: 830 2193 3474 4014 4285 4344 4376 4753 4988
Size: 1363 1281 540 271 59 32 377 235
Cuts at: 4988 830
Size: 3478
Fragments arranged by size:
3478 1363 1281 540 377 271 235 59
32
PvuI CG_AT'CG
Cuts at: 2001 4578 2001
Size: 2577 5059
PvuII CAG'CTG
Cuts at: 3490 3847 3490
Size: 357 7279
SacI G_AGCT'C
Cuts at: 2523 3534 3566 2523
Size: 1011 32 6593
Fragments arranged by size:
6593 1011 32
SacII CC_GC'GG
Cuts at: 2932 2932
Size: 7636
SalI G'TCGA_C
Cuts at: 1936 4978 1936
Size: 3042 4594
SanDI GG'GwC_CC
Cuts at: 4773 4773
Size: 7636
SapI GCTCTTCn'nnn_
Cuts at: 2205 2205
Size: 7636
SbfI CC_TGCA'GG
Cuts at: 2193 4988 2193
Size: 2795 4841
ScaI AGT'ACT
Cuts at: 5884 7596 5884
Size: 1712 5924
SexAI A'CCwGG_T
Cuts at: 3219 3219
Size: 7636
SfcI C'TryA_G
Cuts at: 826 1045 2189 3470 4010 4281 4340 4372 4749
Size: 219 1144 1281 540 271 59 32 377
Cuts at: 4749 4984 6119 6800 6991 826
Size: 235 1135 681 191 1471
Fragments arranged by size:
1471 1281 1144 1135 681 540 377 271
235 219 191 59 32
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7636
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 7636
SmaI CCC'GGG
Cuts at: 1872 4963 1872
Size: 3091 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3067 3326 5740 6641 6903 820
Size: 197 926 1124 259 2414 901 262 1553
Fragments arranged by size:
2414 1553 1124 926 901 262 259 197
SnaBI TAC'GTA
Cuts at: 493 4303 5427 493
Size: 3810 1124 2702
Fragments arranged by size:
3810 2702 1124
SpeI A'CTAG_T
Cuts at: 152 152
Size: 7636
SphI G_CATG'C
Cuts at: 1132 2135 3409 4994 1132
Size: 1003 1274 1585 3774
Fragments arranged by size:
3774 1585 1274 1003
SspI AAT'ATT
Cuts at: 5 52 5560 5
Size: 47 5508 2081
Fragments arranged by size:
5508 2081 47
StuI AGG'CCT
Cuts at: 1517 2280 1517
Size: 763 6873
StyI C'CwwG_G
Cuts at: 513 1065 2817 3636 3723 3739 4845 5075 513
Size: 552 1752 819 87 16 1106 230 3074
Fragments arranged by size:
3074 1752 1106 819 552 230 87 16
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3364 3516 4355 4407 5663 5680 5833
Size: 1255 1784 152 839 52 1256 17 153
Cuts at: 5833 6018 6463 325
Size: 185 445 1498
Fragments arranged by size:
1784 1498 1256 1255 839 445 185 153
152 52 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3918 5882 7594
Size: 274 80 33 51 1443 1941 1964 1712
Cuts at: 7594 96
Size: 138
Fragments arranged by size:
1964 1941 1712 1443 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2585 2617 2639 2671 4028 4060 4068 4100 7596
Size: 32 22 32 1357 32 8 32 3496
Cuts at: 7596 7628 2585
Size: 32 2593
Fragments arranged by size:
3496 2593 1357 32 32 32 32 32
22 8
Tth111I GACn'n_nGTC
Cuts at: 1316 2419 1316
Size: 1103 6533
XbaI T'CTAG_A
Cuts at: 4972 4972
Size: 7636
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2308 3730 4027 4360 2308
Size: 1422 297 333 5584
Fragments arranged by size:
5584 1422 333 297
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 7636
XmaI C'CCGG_G
Cuts at: 1870 4961 1870
Size: 3091 4545
XmnI GAAnn'nnTTC
Cuts at: 5765 5765
Size: 7636
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 2650 3022 4411 5444
Size: 53 83 186 936 1116 372 1389 1033
Cuts at: 5444 276
Size: 2468
Fragments arranged by size:
2468 1389 1116 1033 936 372 186 83
53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI
BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII
BstXI BstYI BstZ17I Bsu36I BtgI BtgZI BtsI ClaI
CspCI DraI DraIII DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII
HindIII HpaI KpnI MfeI MluI MmeI MscI MslI
MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NsiI
NspI PasI PciI PflMI PfoI PmeI PmlI PpiI
PpuMI PshAI PsiI PspOMI PsrI PstI PvuI PvuII
SacI SacII SalI SanDI SapI SbfI ScaI SexAI
SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI AscI AvrII BaeI BbeI BsiWI
EcoNI EcoRV FalI FseI FspAI KasI NarI NruI
PacI PspXI RsrII SfiI SfoI SrfI SwaI