Restriction map for
pFN21AB5539
(Circular) (Six-base) MAPSORT of: pFN21AB5539.seq Check: 9195 from: 1 to: 6388
pFN21AB5539 6388 bp
With 182 enzymes: SgfI *
April 15, 2010 09:31 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4198 278
Size: 53 83 186 936 2662 2468
Fragments arranged by size:
2662 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2009 2934 3731 1937
Size: 72 925 797 4594
Fragments arranged by size:
4594 925 797 72
Acc65I G'GTAC_C
Cuts at: 3709 3709
Size: 6388
AclI AA'CG_TT
Cuts at: 1563 4515 4888 1563
Size: 2952 373 3063
Fragments arranged by size:
3063 2952 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2871 2887 3072 3221 3513 4451 5835 6103
Size: 1239 16 185 149 292 938 1384 268
Cuts at: 6103 1632
Size: 1917
Fragments arranged by size:
1917 1384 1239 938 292 268 185 149
16
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6191
AflIII A'CryG_T
Cuts at: 1203 3525 5287 6264 1203
Size: 2322 1762 977 1327
Fragments arranged by size:
2322 1762 1327 977
AgeI A'CCGG_T
Cuts at: 5219 5219
Size: 6388
AhdI GACnn_n'nnGTC
Cuts at: 5117 5117
Size: 6388
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6388
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6388
AlwNI CAG_nnn'CTG
Cuts at: 2359 2381 3641 4131 5703 2359
Size: 22 1260 490 1572 3044
Fragments arranged by size:
3044 1572 1260 490 22
ApaLI G'TGCA_C
Cuts at: 4445 5601 4445
Size: 1156 5232
ApoI r'AATT_y
Cuts at: 1493 3697 3923 4075 6184 1493
Size: 2204 226 152 2109 1697
Fragments arranged by size:
2204 2109 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4942 160
Size: 872 3910 1606
Fragments arranged by size:
3910 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6388
AvaI C'yCGr_G
Cuts at: 1870 1943 3123 3713 1870
Size: 73 1180 590 4545
Fragments arranged by size:
4545 1180 590 73
BamHI G'GATC_C
Cuts at: 2070 2970 3718 4085 2070
Size: 900 748 367 4373
Fragments arranged by size:
4373 900 748 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3134 3709 5164 618
Size: 325 217 627 1347 575 1455 1842
Fragments arranged by size:
1842 1455 1347 627 575 325 217
BanII G_rGCy'C
Cuts at: 1391 3124 3217 3596 3707 1391
Size: 1733 93 379 111 4072
Fragments arranged by size:
4072 1733 379 111 93
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2679 3060 3501 928
Size: 971 780 381 441 3815
Fragments arranged by size:
3815 971 780 441 381
BbvCI CC'TCA_GC
Cuts at: 3626 3626
Size: 6388
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4579 4613 1903
Size: 34 2642 34 3678
Fragments arranged by size:
3678 2642 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3467 4280 5490 3467
Size: 813 1210 4365
Fragments arranged by size:
4365 1210 813
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6388
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4999 136
Size: 107 122 71 631 605 3327 1525
Fragments arranged by size:
3327 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2405 3366 3408 6383 2405
Size: 961 42 2975 2410
Fragments arranged by size:
2975 2410 961 42
BlpI GC'TnA_GC
Cuts at: 3805 3805
Size: 6388
Bme1580I G_kGCm'C
Cuts at: 1790 4449 5605 1790
Size: 2659 1156 2573
Fragments arranged by size:
2659 2573 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6388
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2369 2886 3396
Size: 416 53 83 399 24 952 517 510
Cuts at: 3396 4119 5077 442
Size: 723 958 1753
Fragments arranged by size:
1753 958 952 723 517 510 416 399
83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6388
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2634 2634
Size: 6388
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2487 2597 2718 3618 5048 1239
Size: 1248 110 121 900 1430 2579
Fragments arranged by size:
2579 1430 1248 900 121 110
Bpu10I CC'TnA_GC
Cuts at: 3233 3626 3233
Size: 393 5995
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4513 5378 5676 4513
Size: 865 298 5225
Fragments arranged by size:
5225 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3575 5051 882
Size: 617 2076 1476 2219
Fragments arranged by size:
2219 2076 1476 617
BsaAI yAC'GTr
Cuts at: 493 1204 4179 493
Size: 711 2975 2702
Fragments arranged by size:
2975 2702 711
BsaBI GATnn'nnATC
Cuts at: 2975 6382 2975
Size: 3407 2981
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4195 4577 5212 275
Size: 53 83 186 936 2662 382 635 1451
Fragments arranged by size:
2662 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4821 5219 5493 5640 6051 4821
Size: 398 274 147 411 5158
Fragments arranged by size:
5158 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 3066 3096 1178
Size: 30 165 30 1663 30 4470
Fragments arranged by size:
4470 1663 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2751 2790 1186
Size: 1565 39 4784
Fragments arranged by size:
4784 1565 39
BseYI C'CCAG_C
Cuts at: 2074 3027 5591 6087 2074
Size: 953 2564 496 2375
Fragments arranged by size:
2564 2375 953 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2730 1163
Size: 573 994 4821
Fragments arranged by size:
4821 994 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3838 4599 5627 1540
Size: 275 186 1837 761 1028 2301
Fragments arranged by size:
2301 1837 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2257 2955 3596 4449 4534 5605 2257
Size: 698 641 853 85 1071 3040
Fragments arranged by size:
3040 1071 853 698 641 85
BsmI GAATG_Cn'
Cuts at: 3899 3992 3899
Size: 93 6295
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2257 2955 3124 3217 3596 3707 4449
Size: 399 467 698 169 93 379 111 742
Cuts at: 4449 4534 5605 1391
Size: 85 1071 2174
Fragments arranged by size:
2174 1071 742 698 467 399 379 169
111 93 85
BspEI T'CCGG_A
Cuts at: 6051 6051
Size: 6388
BspHI T'CATG_A
Cuts at: 4277 6007 4277
Size: 1730 4658
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2100 3743 844
Size: 1256 1643 3489
Fragments arranged by size:
3489 1643 1256
BsrBI CCG'CTC
Cuts at: 1394 1823 2134 4275 1394
Size: 429 311 2141 3507
Fragments arranged by size:
3507 2141 429 311
BsrDI GCAATG_nn'
Cuts at: 65 1248 3436 4883 5057 65
Size: 1183 2188 1447 174 1396
Fragments arranged by size:
2188 1447 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2211 5032 5219 1700
Size: 110 401 2821 187 2869
Fragments arranged by size:
2869 2821 401 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6388
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6388
BssSI C'ACGA_G
Cuts at: 4448 5460 4448
Size: 1012 5376
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2090 3275 794
Size: 1296 1185 3907
Fragments arranged by size:
3907 1296 1185
BstBI TT'CG_AA
Cuts at: 5192 5192
Size: 6388
BstEII G'GTnAC_C
Cuts at: 1184 3287 1184
Size: 2103 4285
BstXI CCAn_nnnn'nTGG
Cuts at: 2712 2981 2712
Size: 269 6119
BstYI r'GATC_y
Cuts at: 1972 2070 2355 2405 2970 3366 3390 3408 3718
Size: 98 285 50 565 396 24 18 310
Cuts at: 3718 4085 4470 4487 5939 6025 6037 6383 1972
Size: 367 385 17 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 565 396 385 367 346 310
285 98 86 50 24 18 17 12
Bsu36I CC'TnA_GG
Cuts at: 3209 3283 3209
Size: 74 6314
BtgI C'CryG_G
Cuts at: 513 1065 513
Size: 552 5836
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2301 487
Size: 683 56 1075 4574
Fragments arranged by size:
4574 1075 683 56
BtsI GCAGTG_nn'
Cuts at: 3904 4698 4718 3904
Size: 794 20 5574
Fragments arranged by size:
5574 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6388
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6353
DraI TTT'AAA
Cuts at: 3692 3692
Size: 6388
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2035 5395 809
Size: 507 719 3360 1802
Fragments arranged by size:
3360 1802 719 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2209 3401 3540
Size: 54 1425 50 275 18 379 1192 139
Cuts at: 3540 3835 4724 6353 8
Size: 295 889 1629 43
Fragments arranged by size:
1629 1425 1192 889 379 295 275 139
54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3835 1537
Size: 275 2023 4090
Fragments arranged by size:
4090 2023 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2124 2603 2662 3294 4318 1353
Size: 771 479 59 632 1024 3423
Fragments arranged by size:
3423 1024 771 632 479 59
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4975 5349 5495 387
Size: 1064 138 3386 374 146 1280
Fragments arranged by size:
3386 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 3594 3594
Size: 6388
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2487 2597 2718 2871 2887 3072 3221
Size: 393 855 110 121 153 16 185 149
Cuts at: 3221 3513 3618 4451 5048 5835 6103 1239
Size: 292 105 833 597 787 268 1524
Fragments arranged by size:
1524 855 833 787 597 393 292 268
185 153 149 121 110 105 16
EcoO109I rG'GnC_Cy
Cuts at: 2638 3127 3205 2638
Size: 489 78 5821
Fragments arranged by size:
5821 489 78
EcoRI G'AATT_C
Cuts at: 3697 4075 3697
Size: 378 6010
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3430 3430
Size: 6388
FspI TGC'GCA
Cuts at: 4894 4894
Size: 6388
HaeII r_GCGC'y
Cuts at: 1678 5535 1678
Size: 3857 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2239 2271 2477 2509 3111
Size: 32 135 32 667 32 206 32 602
Cuts at: 3111 3143 5029 5061 5103 5135 1373
Size: 32 1886 32 42 32 2626
Fragments arranged by size:
2626 1886 667 602 206 135 42 32
32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3172 3732 3978 669
Size: 988 281 1234 560 246 3079
Fragments arranged by size:
3079 1234 988 560 281 246
HindIII A'AGCT_T
Cuts at: 748 3008 3306 748
Size: 2260 298 3830
Fragments arranged by size:
3830 2260 298
HpaI GTT'AAC
Cuts at: 3978 3978
Size: 6388
KpnI G_GTAC'C
Cuts at: 3713 3713
Size: 6388
MfeI C'AATT_G
Cuts at: 2999 3480 3987 2999
Size: 481 507 5400
Fragments arranged by size:
5400 507 481
MluI A'CGCG_T
Cuts at: 6264 6264
Size: 6388
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5502 5686 1305
Size: 4197 184 2007
Fragments arranged by size:
4197 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 3403 3542 10
Size: 54 1425 343 1571 139 2856
Fragments arranged by size:
2856 1571 1425 343 139 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2789 2916 4346 4705 4864 518
Size: 648 1623 127 1430 359 159 2042
Fragments arranged by size:
2042 1623 1430 648 359 159 127
MspA1I CmG'CkG
Cuts at: 1606 2236 3804 4481 5629 5874 1606
Size: 630 1568 677 1148 245 2120
Fragments arranged by size:
2120 1568 1148 677 630 245
NaeI GCC'GGC
Cuts at: 2213 2213
Size: 6388
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5836
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6388
NgoMIV G'CCGG_C
Cuts at: 2211 2211
Size: 6388
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6388
NotI GC'GGCC_GC
Cuts at: 3835 3835
Size: 6388
NspI r_CATG'y
Cuts at: 1132 1229 3071 3529 3746 5291 1132
Size: 97 1842 458 217 1545 2229
Fragments arranged by size:
2229 1842 1545 458 217 97
PasI CC'CwG_GG
Cuts at: 1348 2059 1348
Size: 711 5677
PciI A'CATG_T
Cuts at: 3525 5287 3525
Size: 1762 4626
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6388
PmeI GTTT'AAAC
Cuts at: 3692 3692
Size: 6388
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4457 4489 5970 6002 4457
Size: 32 1481 32 4843
Fragments arranged by size:
4843 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2638 3127 3205 2638
Size: 489 78 5821
Fragments arranged by size:
5821 489 78
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6388
PsiI TTA'TAA
Cuts at: 2525 3958 2525
Size: 1433 4955
PstI C_TGCA'G
Cuts at: 830 2629 3740 830
Size: 1799 1111 3478
Fragments arranged by size:
3478 1799 1111
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6388
PvuII CAG'CTG
Cuts at: 2236 2236
Size: 6388
SacI G_AGCT'C
Cuts at: 3596 3596
Size: 6388
SalI G'TCGA_C
Cuts at: 1936 3730 1936
Size: 1794 4594
SanDI GG'GwC_CC
Cuts at: 2638 2638
Size: 6388
SapI GCTCTTCn'nnn_
Cuts at: 2124 2124
Size: 6388
SbfI CC_TGCA'GG
Cuts at: 3740 3740
Size: 6388
ScaI AGT'ACT
Cuts at: 4636 6348 4636
Size: 1712 4676
SfcI C'TryA_G
Cuts at: 826 1045 2625 3736 4871 5552 5743 826
Size: 219 1580 1111 1135 681 191 1471
Fragments arranged by size:
1580 1471 1135 1111 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6388
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6388
SmaI CCC'GGG
Cuts at: 1872 3125 3715 1872
Size: 1253 590 4545
Fragments arranged by size:
4545 1253 590
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4492 5393 5655 820
Size: 197 926 2549 901 262 1553
Fragments arranged by size:
2549 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 4179 493
Size: 3686 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6388
SphI G_CATG'C
Cuts at: 1132 3746 1132
Size: 2614 3774
SspI AAT'ATT
Cuts at: 5 52 2227 4312 5
Size: 47 2175 2085 2081
Fragments arranged by size:
2175 2085 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6388
StyI C'CwwG_G
Cuts at: 513 1065 2065 3138 3827 513
Size: 552 1000 1073 689 3074
Fragments arranged by size:
3074 1073 1000 689 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2782 4415 4432 4585 4770 5215 325
Size: 1255 1202 1633 17 153 185 445 1498
Fragments arranged by size:
1633 1498 1255 1202 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3463 4634 6346
Size: 274 80 33 51 1443 1486 1171 1712
Cuts at: 6346 96
Size: 138
Fragments arranged by size:
1712 1486 1443 1171 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6348 6380 6348
Size: 32 6356
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6388
XbaI T'CTAG_A
Cuts at: 3724 3724
Size: 6388
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2731 2731
Size: 6388
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6388
XmaI C'CCGG_G
Cuts at: 1870 3123 3713 1870
Size: 1253 590 4545
Fragments arranged by size:
4545 1253 590
XmnI GAAnn'nnTTC
Cuts at: 2601 4517 2601
Size: 1916 4472
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4196 276
Size: 53 83 186 936 2662 2468
Fragments arranged by size:
2662 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I
BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI
FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI
MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI
NgoMIV NheI NotI NspI PasI PciI PflMI PmeI
PpiI PpuMI PshAI PsiI PstI PvuI PvuII SacI
SalI SanDI SapI SbfI ScaI SfcI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BfrBI BsiWI BsmBI BstZ17I DraIII EcoNI EcoRV FseI
FspAI KasI NarI NruI NsiI PacI PfoI PmlI
PspOMI PspXI PsrI RsrII SacII SexAI SfiI SfoI
SrfI SwaI