Restriction map for
pFN21AB5527
(Circular) (Six-base) MAPSORT of: pFN21AB5527.seq Check: 8447 from: 1 to: 6973
pFN21AB5527 6973 bp
With 182 enzymes: SgfI *
October 20, 2009 10:45 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 3225 3225
Size: 6973
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4783 278
Size: 53 83 186 936 3247 2468
Fragments arranged by size:
3247 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2886 4316 1937
Size: 949 1430 4594
Fragments arranged by size:
4594 1430 949
Acc65I G'GTAC_C
Cuts at: 4294 4294
Size: 6973
AclI AA'CG_TT
Cuts at: 1563 5100 5473 1563
Size: 3537 373 3063
Fragments arranged by size:
3537 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2093 2111 2531 2542 2848 3003 3317 3335
Size: 461 18 420 11 306 155 314 18
Cuts at: 3335 3833 5036 6420 6688 1632
Size: 498 1203 1384 268 1917
Fragments arranged by size:
1917 1384 1203 498 461 420 314 306
268 155 18 18 11
AfeI AGC'GCT
Cuts at: 2123 2774 2123
Size: 651 6322
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6776
AflIII A'CryG_T
Cuts at: 1203 3765 5872 6849 1203
Size: 2562 2107 977 1327
Fragments arranged by size:
2562 2107 1327 977
AgeI A'CCGG_T
Cuts at: 5804 5804
Size: 6973
AhdI GACnn_n'nnGTC
Cuts at: 5702 5702
Size: 6973
AleI CACnn'nnGTG
Cuts at: 1166 2880 3377 1166
Size: 1714 497 4762
Fragments arranged by size:
4762 1714 497
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2195 3237 1184
Size: 1011 1042 4920
Fragments arranged by size:
4920 1042 1011
AlwNI CAG_nnn'CTG
Cuts at: 2068 2089 2747 3925 4716 6288 2068
Size: 21 658 1178 791 1572 2753
Fragments arranged by size:
2753 1572 1178 791 658 21
ApaI G_GGCC'C
Cuts at: 2973 3048 3236 3876 2973
Size: 75 188 640 6070
Fragments arranged by size:
6070 640 188 75
ApaLI G'TGCA_C
Cuts at: 5030 6186 5030
Size: 1156 5817
ApoI r'AATT_y
Cuts at: 1493 3736 3949 4282 4508 4660 6769 1493
Size: 2243 213 333 226 152 2109 1697
Fragments arranged by size:
2243 2109 1697 333 226 213 152
AseI AT'TA_AT
Cuts at: 160 1032 5527 160
Size: 872 4495 1606
Fragments arranged by size:
4495 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6973
AvaI C'yCGr_G
Cuts at: 1870 1943 2011 2279 3329 4298 1870
Size: 73 68 268 1050 969 4545
Fragments arranged by size:
4545 1050 969 268 73 68
BamHI G'GATC_C
Cuts at: 4303 4670 4303
Size: 367 6606
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2238 3213 3536 3639 3842
Size: 325 217 627 451 975 323 103 203
Cuts at: 3842 4294 5749 618
Size: 452 1455 1842
Fragments arranged by size:
1842 1455 975 627 452 451 325 323
217 203 103
BanII G_rGCy'C
Cuts at: 1391 2520 2675 2784 2973 3048 3236 3339 3480
Size: 1129 155 109 189 75 188 103 141
Cuts at: 3480 3876 4292 1391
Size: 396 416 4072
Fragments arranged by size:
4072 1129 416 396 189 188 155 141
109 103 75
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2296 3396 928
Size: 971 397 1100 4505
Fragments arranged by size:
4505 1100 971 397
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2525 2559 5164 5198 1903
Size: 34 588 34 2605 34 3678
Fragments arranged by size:
3678 2605 588 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4184 4865 6075 4184
Size: 681 1210 5082
Fragments arranged by size:
5082 1210 681
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6973
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 3140 5584 136
Size: 107 122 71 631 605 1468 2444 1525
Fragments arranged by size:
2444 1525 1468 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2553 4257 6968 2553
Size: 1704 2711 2558
Fragments arranged by size:
2711 2558 1704
BlpI GC'TnA_GC
Cuts at: 2909 4390 2909
Size: 1481 5492
Bme1580I G_kGCm'C
Cuts at: 1790 2241 2280 2973 3001 3048 3236 3589 3642
Size: 451 39 693 28 47 188 353 53
Cuts at: 3642 3876 3886 5034 6190 1790
Size: 234 10 1148 1156 2573
Fragments arranged by size:
2573 1156 1148 693 451 353 234 188
53 47 39 28 10
BmgBI CAC'GTC
Cuts at: 1318 3766 4003 1318
Size: 2448 237 4288
Fragments arranged by size:
4288 2448 237
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2374 4704 5662
Size: 416 53 83 399 24 957 2330 958
Cuts at: 5662 442
Size: 1753
Fragments arranged by size:
2330 1753 958 957 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 2966 3470 1056
Size: 1910 504 4559
Fragments arranged by size:
4559 1910 504
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2276 2612 2972 3044 3191 3209 3369 3511
Size: 1037 336 360 72 147 18 160 142
Cuts at: 3511 4250 5633 1239
Size: 739 1383 2579
Fragments arranged by size:
2579 1383 1037 739 360 336 160 147
142 72 18
Bpu10I CC'TnA_GC
Cuts at: 2674 3113 3288 3461 3644 3867 2674
Size: 439 175 173 183 223 5780
Fragments arranged by size:
5780 439 223 183 175 173
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4166 5098 5963 6261 4166
Size: 932 865 298 4878
Fragments arranged by size:
4878 932 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3825 5636 882
Size: 617 2326 1811 2219
Fragments arranged by size:
2326 2219 1811 617
BsaAI yAC'GTr
Cuts at: 493 1204 3808 4764 493
Size: 711 2604 956 2702
Fragments arranged by size:
2702 2604 956 711
BsaBI GATnn'nnATC
Cuts at: 2055 6967 2055
Size: 4912 2061
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3143 3566 4780 5162
Size: 53 83 186 936 1610 423 1214 382
Cuts at: 5162 5797 275
Size: 635 1451
Fragments arranged by size:
1610 1451 1214 936 635 423 382 186
83 53
BsaWI w'CCGG_w
Cuts at: 5406 5804 6078 6225 6636 5406
Size: 398 274 147 411 5743
Fragments arranged by size:
5743 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2387 2417 2672 2702 4055
Size: 30 165 30 984 30 255 30 1353
Cuts at: 4055 4085 1178
Size: 30 4066
Fragments arranged by size:
4066 1353 984 255 165 30 30 30
30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2128 2615 2747 3439 3670 4123 1186
Size: 942 487 132 692 231 453 4036
Fragments arranged by size:
4036 942 692 487 453 231 132
BseYI C'CCAG_C
Cuts at: 2196 2358 2371 2631 2783 2972 3036 6176 6672
Size: 162 13 260 152 189 64 3140 496
Cuts at: 6672 2196
Size: 2497
Fragments arranged by size:
3140 2497 496 260 189 162 152 64
13
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2018 2363 3166 3455 1163
Size: 573 282 345 803 289 4681
Fragments arranged by size:
4681 803 573 345 289 282
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2650 4423 5184 6212 1540
Size: 275 186 649 1773 761 1028 2301
Fragments arranged by size:
2301 1773 1028 761 649 275 186
BsiHKAI G_wGCw'C
Cuts at: 2520 3114 3480 5034 5119 6190 2520
Size: 594 366 1554 85 1071 3303
Fragments arranged by size:
3303 1554 1071 594 366 85
BsmI GAATG_Cn'
Cuts at: 4484 4577 4484
Size: 93 6880
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2241 2280 2520 2675 2784 2973 3001
Size: 399 451 39 240 155 109 189 28
Cuts at: 3001 3048 3114 3236 3339 3480 3589 3642 3876
Size: 47 66 122 103 141 109 53 234
Cuts at: 3876 3886 4292 5034 5119 6190 1391
Size: 10 406 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 451 406 399 240 234
189 155 141 122 109 109 103 85
66 53 47 39 28 10
BspEI T'CCGG_A
Cuts at: 6636 6636
Size: 6973
BspHI T'CATG_A
Cuts at: 4862 6592 4862
Size: 1730 5243
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2955 3225 4328 844
Size: 2111 270 1103 3489
Fragments arranged by size:
3489 2111 1103 270
BsrBI CCG'CTC
Cuts at: 1394 1823 4860 1394
Size: 429 3037 3507
Fragments arranged by size:
3507 3037 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 5468 5642 65
Size: 1183 4220 174 1396
Fragments arranged by size:
4220 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 5617 5804 1700
Size: 110 3807 187 2869
Fragments arranged by size:
3807 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6973
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6973
BssSI C'ACGA_G
Cuts at: 5033 6045 5033
Size: 1012 5961
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2368 2747 3012 3925 794
Size: 1574 379 265 913 3842
Fragments arranged by size:
3842 1574 913 379 265
BstBI TT'CG_AA
Cuts at: 5777 5777
Size: 6973
BstEII G'GTnAC_C
Cuts at: 1184 3265 1184
Size: 2081 4892
BstXI CCAn_nnnn'nTGG
Cuts at: 3749 3749
Size: 6973
BstYI r'GATC_y
Cuts at: 1972 2056 2553 3093 3306 3981 4257 4303 4670
Size: 84 497 540 213 675 276 46 367
Cuts at: 4670 5055 5072 6524 6610 6622 6968 1972
Size: 385 17 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 675 540 497 385 367 346
276 213 86 84 46 17 12
Bsu36I CC'TnA_GG
Cuts at: 3366 3366
Size: 6973
BtgI C'CryG_G
Cuts at: 513 1065 2004 2406 2667 3070 3696 3985 4049
Size: 552 939 402 261 403 626 289 64
Cuts at: 4049 513
Size: 3437
Fragments arranged by size:
3437 939 626 552 403 402 289 261
64
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2320 2328 3120 487
Size: 683 56 1094 8 792 4340
Fragments arranged by size:
4340 1094 792 683 56 8
BtsI GCAGTG_nn'
Cuts at: 3178 3426 4489 5283 5303 3178
Size: 248 1063 794 20 4848
Fragments arranged by size:
4848 1063 794 248 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6973
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 4231 4266 529
Size: 35 3667 35 3236
Fragments arranged by size:
3667 3236 35 35
DraI TTT'AAA
Cuts at: 4277 4277
Size: 6973
DraIII CAC_nnn'GTG
Cuts at: 2368 3634 2368
Size: 1266 5707
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2191 5980 809
Size: 507 875 3789 1802
Fragments arranged by size:
3789 1802 875 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 4005 4153 4420
Size: 54 1425 50 275 18 2175 148 267
Cuts at: 4420 5309 6938 8
Size: 889 1629 43
Fragments arranged by size:
2175 1629 1425 889 275 267 148 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 4420 1537
Size: 275 2608 4090
Fragments arranged by size:
4090 2608 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2935 3104 4903 1353
Size: 1582 169 1799 3423
Fragments arranged by size:
3423 1799 1582 169
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3614 5560 5934 6080 387
Size: 1064 138 2025 1946 374 146 1280
Fragments arranged by size:
2025 1946 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2518 3478 2518
Size: 960 6013
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2093 2111 2276 2531 2542 2612 2848
Size: 393 461 18 165 255 11 70 236
Cuts at: 2848 2972 3003 3044 3191 3209 3317 3335 3369
Size: 124 31 41 147 18 108 18 34
Cuts at: 3369 3511 3833 4250 5036 5633 6420 6688 1239
Size: 142 322 417 786 597 787 268 1524
Fragments arranged by size:
1524 787 786 597 461 417 393 322
268 255 236 165 147 142 124 108
70 41 34 31 18 18 18 11
EcoNI CCTnn'n_nnAGG
Cuts at: 2951 3314 3866 2951
Size: 363 552 6058
Fragments arranged by size:
6058 552 363
EcoO109I rG'GnC_Cy
Cuts at: 2744 3233 3795 3872 3873 2744
Size: 489 562 77 1 5844
Fragments arranged by size:
5844 562 489 77 1
EcoRI G'AATT_C
Cuts at: 3949 4282 4660 3949
Size: 333 378 6262
Fragments arranged by size:
6262 378 333
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2503 2842 3115 3838 4162 2503
Size: 339 273 723 324 5314
Fragments arranged by size:
5314 723 339 324 273
FspI TGC'GCA
Cuts at: 5479 5479
Size: 6973
HaeII r_GCGC'y
Cuts at: 1678 2069 2125 2776 2862 6120 1678
Size: 391 56 651 86 3258 2531
Fragments arranged by size:
3258 2531 651 391 86 56
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2672 2704 3551 3583 4118
Size: 32 135 32 1100 32 847 32 535
Cuts at: 4118 4150 5614 5646 5688 5720 1373
Size: 32 1464 32 42 32 2626
Fragments arranged by size:
2626 1464 1100 847 535 135 42 32
32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3937 4317 4563 669
Size: 988 281 1999 380 246 3079
Fragments arranged by size:
3079 1999 988 380 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6973
HpaI GTT'AAC
Cuts at: 4563 4563
Size: 6973
KpnI G_GTAC'C
Cuts at: 4298 4298
Size: 6973
MfeI C'AATT_G
Cuts at: 3954 4572 3954
Size: 618 6355
MluI A'CGCG_T
Cuts at: 6849 6849
Size: 6973
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3708 6087 6271 1305
Size: 2403 2379 184 2007
Fragments arranged by size:
2403 2379 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 4007 4155 10
Size: 54 1425 343 2175 148 2828
Fragments arranged by size:
2828 2175 1425 343 148 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2528 2880 3219 3377 4931 5290 5449
Size: 648 1362 352 339 158 1554 359 159
Cuts at: 5449 518
Size: 2042
Fragments arranged by size:
2042 1554 1362 648 359 352 339 159
158
MspA1I CmG'CkG
Cuts at: 1606 2200 2289 2351 2635 2669 2728 2806 3925
Size: 594 89 62 284 34 59 78 1119
Cuts at: 3925 4389 5066 6214 6459 1606
Size: 464 677 1148 245 2120
Fragments arranged by size:
2120 1148 1119 677 594 464 284 245
89 78 62 59 34
NcoI C'CATG_G
Cuts at: 513 1065 2004 2406 3985 4049 513
Size: 552 939 402 1579 64 3437
Fragments arranged by size:
3437 1579 939 552 402 64
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6973
NheI G'CTAG_C
Cuts at: 1052 2962 3466 1052
Size: 1910 504 4559
Fragments arranged by size:
4559 1910 504
NotI GC'GGCC_GC
Cuts at: 4420 4420
Size: 6973
NspI r_CATG'y
Cuts at: 1132 1229 3224 4331 5876 1132
Size: 97 1995 1107 1545 2229
Fragments arranged by size:
2229 1995 1545 1107 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6973
PciI A'CATG_T
Cuts at: 5872 5872
Size: 6973
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2109 2638 2896 2920 3260 3634 3727 1108
Size: 1001 529 258 24 340 374 93 4354
Fragments arranged by size:
4354 1001 529 374 340 258 93 24
PfoI T'CCnGG_A
Cuts at: 3123 3783 4266 3123
Size: 660 483 5830
Fragments arranged by size:
5830 660 483
PmeI GTTT'AAAC
Cuts at: 4277 4277
Size: 6973
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5042 5074 6555 6587 5042
Size: 32 1481 32 5428
Fragments arranged by size:
5428 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 2415 4022 1613
Size: 802 1607 4564
Fragments arranged by size:
4564 1607 802
PsiI TTA'TAA
Cuts at: 4543 4543
Size: 6973
PspOMI G'GGCC_C
Cuts at: 2969 3044 3232 3872 2969
Size: 75 188 640 6070
Fragments arranged by size:
6070 640 188 75
PstI C_TGCA'G
Cuts at: 830 2502 2718 4325 830
Size: 1672 216 1607 3478
Fragments arranged by size:
3478 1672 1607 216
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6973
PvuII CAG'CTG
Cuts at: 2200 2351 3925 2200
Size: 151 1574 5248
Fragments arranged by size:
5248 1574 151
SacI G_AGCT'C
Cuts at: 2520 3480 2520
Size: 960 6013
SacII CC_GC'GG
Cuts at: 2670 2670
Size: 6973
SalI G'TCGA_C
Cuts at: 1936 4315 1936
Size: 2379 4594
SbfI CC_TGCA'GG
Cuts at: 4325 4325
Size: 6973
ScaI AGT'ACT
Cuts at: 5221 6933 5221
Size: 1712 5261
SexAI A'CCwGG_T
Cuts at: 2919 2919
Size: 6973
SfcI C'TryA_G
Cuts at: 826 1045 2042 2060 2426 2498 2714 3688 4321
Size: 219 997 18 366 72 216 974 633
Cuts at: 4321 5456 6137 6328 826
Size: 1135 681 191 1471
Fragments arranged by size:
1471 1135 997 974 681 633 366 219
216 191 72 18
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6973
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6973
SmaI CCC'GGG
Cuts at: 1872 4300 1872
Size: 2428 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4181 5077 5978 6240 820
Size: 197 926 2238 896 901 262 1553
Fragments arranged by size:
2238 1553 926 901 896 262 197
SnaBI TAC'GTA
Cuts at: 493 4764 493
Size: 4271 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6973
SphI G_CATG'C
Cuts at: 1132 3224 4331 1132
Size: 2092 1107 3774
Fragments arranged by size:
3774 2092 1107
SspI AAT'ATT
Cuts at: 5 52 4897 5
Size: 47 4845 2081
Fragments arranged by size:
4845 2081 47
StuI AGG'CCT
Cuts at: 1517 2612 3551 1517
Size: 1095 939 4939
Fragments arranged by size:
4939 1095 939
StyI C'CwwG_G
Cuts at: 513 1065 2004 2406 2796 2817 3447 3985 4049
Size: 552 939 402 390 21 630 538 64
Cuts at: 4049 4412 513
Size: 363 3074
Fragments arranged by size:
3074 939 630 552 538 402 390 363
64 21
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2356 5000 5017 5170 5355 5800 325
Size: 1255 776 2644 17 153 185 445 1498
Fragments arranged by size:
2644 1498 1255 776 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2835 5219 6931
Size: 274 80 33 51 1443 858 2384 1712
Cuts at: 6931 96
Size: 138
Fragments arranged by size:
2384 1712 1443 858 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2387 2419 6933 6965 2387
Size: 32 4514 32 2395
Fragments arranged by size:
4514 2395 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 2686 3488 1316
Size: 1370 802 4801
Fragments arranged by size:
4801 1370 802
XbaI T'CTAG_A
Cuts at: 4309 4309
Size: 6973
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3973 3973
Size: 6973
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6973
XmaI C'CCGG_G
Cuts at: 1870 4298 1870
Size: 2428 4545
XmnI GAAnn'nnTTC
Cuts at: 2555 4145 5102 2555
Size: 1590 957 4426
Fragments arranged by size:
4426 1590 957
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4781 276
Size: 53 83 186 936 3247 2468
Fragments arranged by size:
3247 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI
Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI
EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII
HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I
NcoI NdeI NheI NotI NspI PasI PciI PflMI
PfoI PmeI PpiI PshAI PsiI PspOMI PstI PvuI
PvuII SacI SacII SalI SbfI ScaI SexAI SfcI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XcmI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AloI AscI AvrII BaeI BbeI BbvCI BfrBI BplI
BsiWI BsmBI BstZ17I EcoRV FseI FspAI KasI NaeI
NarI NgoMIV NruI NsiI PacI PmlI PpuMI PspXI
PsrI RsrII SanDI SapI SfiI SfoI SrfI SwaI