Restriction map for
pFN21AB5523
(Circular) (Six-base) MAPSORT of: pFN21AB5523.seq Check: 1389 from: 1 to: 12121
pFN21AB5523 12121 bp
With 182 enzymes: SgfI *
January 16, 2009 15:25 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 3427 5197 3427
Size: 1770 10351
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 6610 9931 278
Size: 53 83 186 936 5074 3321 2468
Fragments arranged by size:
5074 3321 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 9464 1937
Size: 7527 4594
Acc65I G'GTAC_C
Cuts at: 9442 9442
Size: 12121
AclI AA'CG_TT
Cuts at: 1563 9420 10248 10621 1563
Size: 7857 828 373 3063
Fragments arranged by size:
7857 3063 828 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2180 2184 2234 2295 2547 2744 3213 4811
Size: 548 4 50 61 252 197 469 1598
Cuts at: 4811 5444 5487 5502 7166 7589 7751 7925 10184
Size: 633 43 15 1664 423 162 174 2259
Cuts at: 10184 11568 11836 1632
Size: 1384 268 1917
Fragments arranged by size:
2259 1917 1664 1598 1384 633 548 469
423 268 252 197 174 162 61 50
43 15 4
AfeI AGC'GCT
Cuts at: 2780 3516 5645 6032 2780
Size: 736 2129 387 8869
Fragments arranged by size:
8869 2129 736 387
AflII C'TTAA_G
Cuts at: 820 1017 3521 820
Size: 197 2504 9420
Fragments arranged by size:
9420 2504 197
AflIII A'CryG_T
Cuts at: 1203 8146 11020 11997 1203
Size: 6943 2874 977 1327
Fragments arranged by size:
6943 2874 1327 977
AgeI A'CCGG_T
Cuts at: 10952 10952
Size: 12121
AhdI GACnn_n'nnGTC
Cuts at: 5476 6058 7333 10850 5476
Size: 582 1275 3517 6747
Fragments arranged by size:
6747 3517 1275 582
AleI CACnn'nnGTG
Cuts at: 1166 3225 1166
Size: 2059 10062
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 3425 4707 4885 5150 6338 6540 8930 1184
Size: 2241 1282 178 265 1188 202 2390 4375
Fragments arranged by size:
4375 2390 2241 1282 1188 265 202 178
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 9200 9232 9200
Size: 32 12089
AlwNI CAG_nnn'CTG
Cuts at: 2122 2545 2722 2881 3283 4342 4468 4660 5665
Size: 423 177 159 402 1059 126 192 1005
Cuts at: 5665 5815 6217 6964 7408 7537 7600 8209 9301
Size: 150 402 747 444 129 63 609 1092
Cuts at: 9301 9864 11436 2122
Size: 563 1572 2807
Fragments arranged by size:
2807 1572 1092 1059 1005 747 609 563
444 423 402 402 192 177 159 150
129 126 63
ApaI G_GGCC'C
Cuts at: 3706 9250 3706
Size: 5544 6577
ApaLI G'TGCA_C
Cuts at: 5944 10178 11334 5944
Size: 4234 1156 6731
Fragments arranged by size:
6731 4234 1156
ApoI r'AATT_y
Cuts at: 1493 2573 3587 8984 9430 9656 9808 11917 1493
Size: 1080 1014 5397 446 226 152 2109 1697
Fragments arranged by size:
5397 2109 1697 1080 1014 446 226 152
AseI AT'TA_AT
Cuts at: 160 1032 10675 160
Size: 872 9643 1606
Fragments arranged by size:
9643 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 12121
AvaI C'yCGr_G
Cuts at: 1870 1943 4953 5097 5915 6393 7587 9306 9446
Size: 73 3010 144 818 478 1194 1719 140
Cuts at: 9446 1870
Size: 4545
Fragments arranged by size:
4545 3010 1719 1194 818 478 144 140
73
BamHI G'GATC_C
Cuts at: 6745 6819 8664 9139 9451 9818 6745
Size: 74 1845 475 312 367 9048
Fragments arranged by size:
9048 1845 475 367 312 74
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 9442 10897 618
Size: 325 217 627 7655 1455 1842
Fragments arranged by size:
7655 1842 1455 627 325 217
BanII G_rGCy'C
Cuts at: 1391 3706 3759 3867 4320 4419 7370 9250 9291
Size: 2315 53 108 453 99 2951 1880 41
Cuts at: 9291 9372 9440 1391
Size: 81 68 4072
Fragments arranged by size:
4072 2951 2315 1880 453 108 99 81
68 53 41
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2934 3252 3489 3540 4776 5475 5934
Size: 971 1035 318 237 51 1236 699 459
Cuts at: 5934 6423 6474 8009 8022 928
Size: 489 51 1535 13 5027
Fragments arranged by size:
5027 1535 1236 1035 971 699 489 459
318 237 51 51 13
BbvCI CC'TCA_GC
Cuts at: 2952 4779 6126 8423 9251 2952
Size: 1827 1347 2297 828 5822
Fragments arranged by size:
5822 2297 1827 1347 828
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4037 4071 4757 4791 7174 7208 7691
Size: 34 2100 34 686 34 2383 34 483
Cuts at: 7691 7725 10312 10346 1903
Size: 34 2587 34 3678
Fragments arranged by size:
3678 2587 2383 2100 686 483 34 34
34 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2175 10013 11223 2175
Size: 7838 1210 3073
Fragments arranged by size:
7838 3073 1210
BclI T'GATC_A
Cuts at: 1548 2679 3972 6453 9189 1548
Size: 1131 1293 2481 2736 4480
Fragments arranged by size:
4480 2736 2481 1293 1131
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4292 6398 6902
Size: 107 122 71 631 605 2620 2106 504
Cuts at: 6902 7046 8168 8300 9245 10732 136
Size: 144 1122 132 945 1487 1525
Fragments arranged by size:
2620 2106 1525 1487 1122 945 631 605
504 144 132 122 107 71
BglII A'GATC_T
Cuts at: 2911 4129 4816 12116 2911
Size: 1218 687 7300 2916
Fragments arranged by size:
7300 2916 1218 687
BlpI GC'TnA_GC
Cuts at: 3861 9538 3861
Size: 5677 6444
Bme1580I G_kGCm'C
Cuts at: 1790 3559 3706 5948 9250 10182 11338 1790
Size: 1769 147 2242 3302 932 1156 2573
Fragments arranged by size:
3302 2573 2242 1769 1156 932 147
BmgBI CAC'GTC
Cuts at: 1318 2485 4398 7963 1318
Size: 1167 1913 3565 5476
Fragments arranged by size:
5476 3565 1913 1167
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3040 4914 5080
Size: 416 53 83 399 24 1623 1874 166
Cuts at: 5080 5249 9852 10810 442
Size: 169 4603 958 1753
Fragments arranged by size:
4603 1874 1753 1623 958 416 399 169
166 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 3713 6107 1056
Size: 2657 2394 7070
Fragments arranged by size:
7070 2657 2394
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 7861 7861
Size: 12121
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2192 2567 2924 3383 4136 4682 5132 5375
Size: 953 375 357 459 753 546 450 243
Cuts at: 5375 5495 5986 6158 6314 7907 8264 8549 8560
Size: 120 491 172 156 1593 357 285 11
Cuts at: 8560 8597 8948 10781 1239
Size: 37 351 1833 2579
Fragments arranged by size:
2579 1833 1593 953 753 546 491 459
450 375 357 357 351 285 243 172
156 120 37 11
Bpu10I CC'TnA_GC
Cuts at: 2952 4779 6038 6126 6910 8423 9251 2952
Size: 1827 1259 88 784 1513 828 5822
Fragments arranged by size:
5822 1827 1513 1259 828 784 88
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3743 4295 10246 11111 11409 3743
Size: 552 5951 865 298 4455
Fragments arranged by size:
5951 4455 865 552 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2853 8016 8528 8666 10784 882
Size: 617 1354 5163 512 138 2118 2219
Fragments arranged by size:
5163 2219 2118 1354 617 512 138
BsaAI yAC'GTr
Cuts at: 493 1204 5065 9274 9349 9391 9912 493
Size: 711 3861 4209 75 42 521 2702
Fragments arranged by size:
4209 3861 2702 711 521 75 42
BsaBI GATnn'nnATC
Cuts at: 2133 12115 2133
Size: 9982 2139
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2113 6607 8406 9928
Size: 53 83 186 936 580 4494 1799 1522
Cuts at: 9928 10310 10945 275
Size: 382 635 1451
Fragments arranged by size:
4494 1799 1522 1451 936 635 580 382
186 83 53
BsaWI w'CCGG_w
Cuts at: 6119 6156 6816 10554 10952 11226 11373 11784 6119
Size: 37 660 3738 398 274 147 411 6456
Fragments arranged by size:
6456 3738 660 411 398 274 147 37
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2286 2316 9314 9344 1178
Size: 30 165 30 883 30 6998 30 3955
Fragments arranged by size:
6998 3955 883 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2684 3359 4406 5129 5369 5513 7346 7535
Size: 1498 675 1047 723 240 144 1833 189
Cuts at: 7535 7850 8036 8351 8407 8546 8621 8678 8837
Size: 315 186 315 56 139 75 57 159
Cuts at: 8837 8984 9239 9299 1186
Size: 147 255 60 4008
Fragments arranged by size:
4008 1833 1498 1047 723 675 315 315
255 240 189 186 159 147 144 139
75 60 57 56
BseYI C'CCAG_C
Cuts at: 2533 3129 3185 3705 4644 4680 8072 8637 8733
Size: 596 56 520 939 36 3392 565 96
Cuts at: 8733 8877 8902 11324 11820 2533
Size: 144 25 2422 496 2834
Fragments arranged by size:
3392 2834 2422 939 596 565 520 496
144 96 56 36 25
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2300 2369 2480 4163 5525 6006 6306
Size: 573 564 69 111 1683 1362 481 300
Cuts at: 6306 7286 7421 7777 8057 1163
Size: 980 135 356 280 5227
Fragments arranged by size:
5227 1683 1362 980 573 564 481 356
300 280 135 111 69
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 5038 7384 9571 10332 11360 1540
Size: 275 186 3037 2346 2187 761 1028 2301
Fragments arranged by size:
3037 2346 2301 2187 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3222 3759 3867 5391 5948 7557 8859 9291 9372
Size: 537 108 1524 557 1609 1302 432 81
Cuts at: 9372 10182 10267 11338 3222
Size: 810 85 1071 4005
Fragments arranged by size:
4005 1609 1524 1302 1071 810 557 537
432 108 85 81
BsiWI C'GTAC_G
Cuts at: 5061 5061
Size: 12121
BsmI GAATG_Cn'
Cuts at: 4552 9632 9725 4552
Size: 5080 93 6948
Fragments arranged by size:
6948 5080 93
BsmBI CGTCTCn'nnnn_
Cuts at: 4388 9334 4388
Size: 4946 7175
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 3222 3559 3706 3759 3867 4320 4419
Size: 399 1432 337 147 53 108 453 99
Cuts at: 4419 5391 5948 7370 7557 8859 9250 9291 9372
Size: 972 557 1422 187 1302 391 41 81
Cuts at: 9372 9440 10182 10267 11338 1391
Size: 68 742 85 1071 2174
Fragments arranged by size:
2174 1432 1422 1302 1071 972 742 557
453 399 391 337 187 147 108 99
85 81 68 53 41
BspEI T'CCGG_A
Cuts at: 11784 11784
Size: 12121
BspHI T'CATG_A
Cuts at: 2605 8187 9186 10010 11740 2605
Size: 5582 999 824 1730 2986
Fragments arranged by size:
5582 2986 1730 999 824
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2056 3417 3427 5197 5368 5940 6033 7098
Size: 1212 1361 10 1770 171 572 93 1065
Cuts at: 7098 9087 9476 844
Size: 1989 389 3489
Fragments arranged by size:
3489 1989 1770 1361 1212 1065 572 389
171 93 10
BsrBI CCG'CTC
Cuts at: 1394 1823 3353 5342 8415 9405 10008 1394
Size: 429 1530 1989 3073 990 603 3507
Fragments arranged by size:
3507 3073 1989 1530 990 603 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3630 3676 4066 4419 5324 6131 6589
Size: 1183 2382 46 390 353 905 807 458
Cuts at: 6589 8100 10616 10790 65
Size: 1511 2516 174 1396
Fragments arranged by size:
2516 2382 1511 1396 1183 905 807 458
390 353 174 46
BsrFI r'CCGG_y
Cuts at: 1700 1810 4872 5053 5180 6545 6576 6872 7456
Size: 110 3062 181 127 1365 31 296 584
Cuts at: 7456 10765 10952 1700
Size: 3309 187 2869
Fragments arranged by size:
3309 3062 2869 1365 584 296 187 181
127 110 31
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 12121
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 12121
BssSI C'ACGA_G
Cuts at: 2894 3680 4289 4601 10181 11193 2894
Size: 786 609 312 5580 1012 3822
Fragments arranged by size:
5580 3822 1012 786 609 312
BstAPI GCAn_nnn'nTGC
Cuts at: 794 3287 4502 5512 7108 794
Size: 2493 1215 1010 1596 5807
Fragments arranged by size:
5807 2493 1596 1215 1010
BstBI TT'CG_AA
Cuts at: 10925 10925
Size: 12121
BstEII G'GTnAC_C
Cuts at: 1184 4960 1184
Size: 3776 8345
BstXI CCAn_nnnn'nTGG
Cuts at: 3181 4244 3181
Size: 1063 11058
BstYI r'GATC_y
Cuts at: 1972 2542 2911 3039 3811 4129 4816 6745 6819
Size: 570 369 128 772 318 687 1929 74
Cuts at: 6819 7524 8664 8937 9139 9451 9818 10203 10220
Size: 705 1140 273 202 312 367 385 17
Cuts at: 10220 11672 11758 11770 12116 1972
Size: 1452 86 12 346 1977
Fragments arranged by size:
1977 1929 1452 1140 772 705 687 570
385 369 367 346 318 312 273 202
128 86 74 17 12
Bsu36I CC'TnA_GG
Cuts at: 2108 2667 7409 2108
Size: 559 4742 6820
Fragments arranged by size:
6820 4742 559
BtgI C'CryG_G
Cuts at: 513 1065 2004 4320 4551 4890 5576 5607 6823
Size: 552 939 2316 231 339 686 31 1216
Cuts at: 6823 8517 8817 8865 9084 513
Size: 1694 300 48 219 3550
Fragments arranged by size:
3550 2316 1694 1216 939 686 552 339
300 231 219 48 31
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 6523 7280 487
Size: 683 56 5297 757 5328
Fragments arranged by size:
5328 5297 757 683 56
BtsI GCAGTG_nn'
Cuts at: 3281 3404 5132 5191 6883 7192 8112 8888 9637
Size: 123 1728 59 1692 309 920 776 749
Cuts at: 9637 10431 10451 3281
Size: 794 20 4951
Fragments arranged by size:
4951 1728 1692 920 794 776 749 309
123 59 20
ClaI AT'CG_AT
Cuts at: 1554 2137 6168 1554
Size: 583 4031 7507
Fragments arranged by size:
7507 4031 583
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 12086
DraI TTT'AAA
Cuts at: 9425 9425
Size: 12121
DraIII CAC_nnn'GTG
Cuts at: 5608 5608
Size: 12121
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 7763 9326 11128 809
Size: 507 6447 1563 1802 1802
Fragments arranged by size:
6447 1802 1802 1563 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2854 3636 3907
Size: 54 1425 50 275 18 1024 782 271
Cuts at: 3907 4246 4344 5610 5667 6547 7381 7683 8017
Size: 339 98 1266 57 880 834 302 334
Cuts at: 8017 8211 8454 9154 9568 10457 12086 8
Size: 194 243 700 414 889 1629 43
Fragments arranged by size:
1629 1425 1266 1024 889 880 834 782
700 414 339 334 302 275 271 243
194 98 57 54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 7381 9568 1537
Size: 275 5569 2187 4090
Fragments arranged by size:
5569 4090 2187 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2160 2523 2574 3422 4376 4940 5264 5748
Size: 807 363 51 848 954 564 324 484
Cuts at: 5748 6492 7599 7638 7852 8186 8285 8706 10051
Size: 744 1107 39 214 334 99 421 1345
Cuts at: 10051 1353
Size: 3423
Fragments arranged by size:
3423 1345 1107 954 848 807 744 564
484 421 363 334 324 214 99 51
39
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 7973 10708 11082 11228 387
Size: 1064 138 6384 2735 374 146 1280
Fragments arranged by size:
6384 2735 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 3757 3865 9289 9370 3757
Size: 108 5424 81 6508
Fragments arranged by size:
6508 5424 108 81
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2180 2184 2192 2234 2295 2547 2567
Size: 393 548 4 8 42 61 252 20
Cuts at: 2567 2744 2924 3213 3383 4136 4682 4811 5132
Size: 177 180 289 170 753 546 129 321
Cuts at: 5132 5375 5444 5487 5495 5502 5986 6158 6314
Size: 243 69 43 8 7 484 172 156
Cuts at: 6314 7166 7589 7751 7907 7925 8264 8549 8560
Size: 852 423 162 156 18 339 285 11
Cuts at: 8560 8597 8948 10184 10781 11568 11836 1239
Size: 37 351 1236 597 787 268 1524
Fragments arranged by size:
1524 1236 852 787 753 597 548 546
484 423 393 351 339 321 289 285
268 252 243 180 177 172 170 162
156 156 129 69 61 43 42 37
20 18 11 8 8 7 4
EcoNI CCTnn'n_nnAGG
Cuts at: 3341 4172 6296 3341
Size: 831 2124 9166
Fragments arranged by size:
9166 2124 831
EcoO109I rG'GnC_Cy
Cuts at: 2068 2728 4657 5419 5812 5845 6052 7039 7150
Size: 660 1929 762 393 33 207 987 111
Cuts at: 7150 7387 7861 8275 8524 8697 8721 8803 8827
Size: 237 474 414 249 173 24 82 24
Cuts at: 8827 8974 9247 2068
Size: 147 273 4942
Fragments arranged by size:
4942 1929 987 762 660 474 414 393
273 249 237 207 173 147 111 82
33 24 24
EcoRI G'AATT_C
Cuts at: 8984 9430 9808 8984
Size: 446 378 11297
Fragments arranged by size:
11297 446 378
EcoRV GAT'ATC
Cuts at: 4981 4981
Size: 12121
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3536 8147 3536
Size: 4611 7510
FspI TGC'GCA
Cuts at: 3380 7449 8912 10627 3380
Size: 4069 1463 1715 4874
Fragments arranged by size:
4874 4069 1715 1463
FspAI rTGC'GCAy
Cuts at: 8912 8912
Size: 12121
HaeII r_GCGC'y
Cuts at: 1678 2782 3359 3518 5647 5700 5899 6034 7240
Size: 1104 577 159 2129 53 199 135 1206
Cuts at: 7240 9240 11268 1678
Size: 2000 2028 2531
Fragments arranged by size:
2531 2129 2028 2000 1206 1104 577 199
159 135 53
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 4697 4729 4757 4789 4801
Size: 32 135 32 3125 32 28 32 12
Cuts at: 4801 4833 5140 5172 5462 5494 5781 5813 6044
Size: 32 307 32 290 32 287 32 231
Cuts at: 6044 6076 6313 6345 6496 6528 6620 6652 7319
Size: 32 237 32 151 32 92 32 667
Cuts at: 7319 7351 7794 7826 7913 7945 9035 9067 10762
Size: 32 443 32 87 32 1090 32 1695
Cuts at: 10762 10794 10836 10868 1373
Size: 32 42 32 2626
Fragments arranged by size:
3125 2626 1695 1090 667 443 307 290
287 237 231 151 135 92 87 42
32 32 32 32 32 32 32 32
32 32 32 32 32 32 32 32
32 32 28 12
HincII GTy'rAC
Cuts at: 669 1657 1938 3928 9465 9711 669
Size: 988 281 1990 5537 246 3079
Fragments arranged by size:
5537 3079 1990 988 281 246
HindIII A'AGCT_T
Cuts at: 748 2246 3801 4022 4595 4638 4803 7743 8237
Size: 1498 1555 221 573 43 165 2940 494
Cuts at: 8237 8385 748
Size: 148 4484
Fragments arranged by size:
4484 2940 1555 1498 573 494 221 165
148 43
HpaI GTT'AAC
Cuts at: 9711 9711
Size: 12121
KpnI G_GTAC'C
Cuts at: 9446 9446
Size: 12121
MfeI C'AATT_G
Cuts at: 2514 9720 2514
Size: 7206 4915
MluI A'CGCG_T
Cuts at: 11997 11997
Size: 12121
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2792 3843 5244 5341 6688 8520 11235 11419
Size: 1487 1051 1401 97 1347 1832 2715 184
Cuts at: 11419 1305
Size: 2007
Fragments arranged by size:
2715 2007 1832 1487 1401 1347 1051 184
97
MscI TGG'CCA
Cuts at: 10 64 1489 1832 3638 3909 4248 5612 7685
Size: 54 1425 343 1806 271 339 1364 2073
Cuts at: 7685 8213 9156 10
Size: 528 943 2975
Fragments arranged by size:
2975 2073 1806 1425 1364 943 528 343
339 271 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3225 4254 5724 8912 10079 10438 10597
Size: 648 2059 1029 1470 3188 1167 359 159
Cuts at: 10597 518
Size: 2042
Fragments arranged by size:
3188 2059 2042 1470 1167 1029 648 359
159
MspA1I CmG'CkG
Cuts at: 1606 2719 3129 5665 6217 6349 6947 7534 7576
Size: 1113 410 2536 552 132 598 587 42
Cuts at: 7576 7583 7597 8319 8452 8596 8893 8902 9537
Size: 7 14 722 133 144 297 9 635
Cuts at: 9537 10214 11362 11607 1606
Size: 677 1148 245 2120
Fragments arranged by size:
2536 2120 1148 1113 722 677 635 598
587 552 410 297 245 144 133 132
42 14 9 7
NaeI GCC'GGC
Cuts at: 4874 5055 5182 6547 6578 6874 7458 4874
Size: 181 127 1365 31 296 584 9537
Fragments arranged by size:
9537 1365 584 296 181 127 31
NcoI C'CATG_G
Cuts at: 513 1065 2004 4320 4551 8517 8817 9084 513
Size: 552 939 2316 231 3966 300 267 3550
Fragments arranged by size:
3966 3550 2316 939 552 300 267 231
NdeI CA'TA_TG
Cuts at: 387 387
Size: 12121
NgoMIV G'CCGG_C
Cuts at: 4872 5053 5180 6545 6576 6872 7456 4872
Size: 181 127 1365 31 296 584 9537
Fragments arranged by size:
9537 1365 584 296 181 127 31
NheI G'CTAG_C
Cuts at: 1052 3709 6103 1052
Size: 2657 2394 7070
Fragments arranged by size:
7070 2657 2394
NotI GC'GGCC_GC
Cuts at: 9568 9568
Size: 12121
NspI r_CATG'y
Cuts at: 1132 1229 2555 3068 4166 5263 6806 8911 8917
Size: 97 1326 513 1098 1097 1543 2105 6
Cuts at: 8917 9479 11024 1132
Size: 562 1545 2229
Fragments arranged by size:
2229 2105 1545 1543 1326 1098 1097 562
513 97 6
PasI CC'CwG_GG
Cuts at: 1348 5544 9067 1348
Size: 4196 3523 4402
Fragments arranged by size:
4402 4196 3523
PciI A'CATG_T
Cuts at: 11020 11020
Size: 12121
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2455 2545 5689 5745 8209 1108
Size: 1347 90 3144 56 2464 5020
Fragments arranged by size:
5020 3144 2464 1347 90 56
PfoI T'CCnGG_A
Cuts at: 2049 4374 4715 5109 6048 6492 7314 7337 7473
Size: 2325 341 394 939 444 822 23 136
Cuts at: 7473 7973 8835 9126 2049
Size: 500 862 291 5044
Fragments arranged by size:
5044 2325 939 862 822 500 444 394
341 291 136 23
PmeI GTTT'AAAC
Cuts at: 9425 9425
Size: 12121
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 10190 10222 11703 11735 10190
Size: 32 1481 32 10576
Fragments arranged by size:
10576 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2068 4657 5419 5845 6052 7150 7387 8275 8697
Size: 2589 762 426 207 1098 237 888 422
Cuts at: 8697 8721 8803 2068
Size: 24 82 5386
Fragments arranged by size:
5386 2589 1098 888 762 426 422 237
207 82 24
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 12121
PsiI TTA'TAA
Cuts at: 2220 3807 9691 2220
Size: 1587 5884 4650
Fragments arranged by size:
5884 4650 1587
PspOMI G'GGCC_C
Cuts at: 3702 9246 3702
Size: 5544 6577
PstI C_TGCA'G
Cuts at: 830 2445 2742 3265 3414 3732 4579 4677 5937
Size: 1615 297 523 149 318 847 98 1260
Cuts at: 5937 6030 6348 6519 7900 8082 8586 9473 830
Size: 93 318 171 1381 182 504 887 3478
Fragments arranged by size:
3478 1615 1381 1260 887 847 523 504
318 318 297 182 171 149 98 93
PvuI CG_AT'CG
Cuts at: 2001 5038 2001
Size: 3037 9084
PvuII CAG'CTG
Cuts at: 3129 5665 6217 6349 7576 7583 8319 8452 8893
Size: 2536 552 132 1227 7 736 133 441
Cuts at: 8893 8902 3129
Size: 9 6348
Fragments arranged by size:
6348 2536 1227 736 552 441 133 132
9 7
SacI G_AGCT'C
Cuts at: 3759 3867 9291 9372 3759
Size: 108 5424 81 6508
Fragments arranged by size:
6508 5424 108 81
SalI G'TCGA_C
Cuts at: 1936 9463 1936
Size: 7527 4594
SanDI GG'GwC_CC
Cuts at: 8721 8721
Size: 12121
SapI GCTCTTCn'nnn_
Cuts at: 2160 2523 3422 4376 2160
Size: 363 899 954 9905
Fragments arranged by size:
9905 954 899 363
SbfI CC_TGCA'GG
Cuts at: 5937 9473 5937
Size: 3536 8585
ScaI AGT'ACT
Cuts at: 2465 4688 10369 12081 2465
Size: 2223 5681 1712 2505
Fragments arranged by size:
5681 2505 2223 1712
SexAI A'CCwGG_T
Cuts at: 8507 8507
Size: 12121
SfcI C'TryA_G
Cuts at: 826 1045 2441 2738 3261 3410 3728 4575 4673
Size: 219 1396 297 523 149 318 847 98
Cuts at: 4673 5312 5657 5933 6026 6344 6515 7896 8078
Size: 639 345 276 93 318 171 1381 182
Cuts at: 8078 8549 8582 9469 10604 11285 11476 826
Size: 471 33 887 1135 681 191 1471
Fragments arranged by size:
1471 1396 1381 1135 887 847 681 639
523 471 345 318 318 297 276 219
191 182 171 149 98 93 33
SfiI GGCCn_nnn'nGGCC
Cuts at: 8168 8168
Size: 12121
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 12121
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 12121
SmaI CCC'GGG
Cuts at: 1872 5099 6395 7589 9308 9448 1872
Size: 3227 1296 1194 1719 140 4545
Fragments arranged by size:
4545 3227 1719 1296 1194 140
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3521 3758 4310 10225 11126 11388
Size: 197 926 1578 237 552 5915 901 262
Cuts at: 11388 820
Size: 1553
Fragments arranged by size:
5915 1578 1553 926 901 552 262 237
197
SnaBI TAC'GTA
Cuts at: 493 9912 493
Size: 9419 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 12121
SphI G_CATG'C
Cuts at: 1132 6806 8911 8917 9479 1132
Size: 5674 2105 6 562 3774
Fragments arranged by size:
5674 3774 2105 562 6
SspI AAT'ATT
Cuts at: 5 52 10045 5
Size: 47 9993 2081
Fragments arranged by size:
9993 2081 47
StuI AGG'CCT
Cuts at: 1517 5963 6302 6401 6521 8084 1517
Size: 4446 339 99 120 1563 5554
Fragments arranged by size:
5554 4446 1563 339 120 99
StyI C'CwwG_G
Cuts at: 513 1065 2004 3048 3498 3894 3939 4320 4551
Size: 552 939 1044 450 396 45 381 231
Cuts at: 4551 5878 6108 6558 7611 8157 8370 8517 8817
Size: 1327 230 450 1053 546 213 147 300
Cuts at: 8817 9084 9279 9560 513
Size: 267 195 281 3074
Fragments arranged by size:
3074 1327 1053 1044 939 552 546 450
450 396 381 300 281 267 231 230
213 195 147 45
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2091 3121 4215 5231 5999 10148 10165
Size: 1255 511 1030 1094 1016 768 4149 17
Cuts at: 10165 10318 10503 10948 325
Size: 153 185 445 1498
Fragments arranged by size:
4149 1498 1255 1094 1030 1016 768 511
445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2463 3835 4686
Size: 274 80 33 51 1443 486 1372 851
Cuts at: 4686 5707 8847 10367 12079 96
Size: 1021 3140 1520 1712 138
Fragments arranged by size:
3140 1712 1520 1443 1372 1021 851 486
274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 8138 8170 9124 9156 12081 12113 8138
Size: 32 954 32 2925 32 8146
Fragments arranged by size:
8146 2925 954 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 2933 1316
Size: 1617 10504
XbaI T'CTAG_A
Cuts at: 2923 9457 2923
Size: 6534 5587
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2305 3946 8524 2305
Size: 1641 4578 5902
Fragments arranged by size:
5902 4578 1641
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 12121
XmaI C'CCGG_G
Cuts at: 1870 5097 6393 7587 9306 9446 1870
Size: 3227 1296 1194 1719 140 4545
Fragments arranged by size:
4545 3227 1719 1296 1194 140
XmnI GAAnn'nnTTC
Cuts at: 2193 2583 3516 10250 2193
Size: 390 933 6734 4064
Fragments arranged by size:
6734 4064 933 390
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 6608 9929 276
Size: 53 83 186 936 5074 3321 2468
Fragments arranged by size:
5074 3321 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI
Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI BsiWI BsmI BsmBI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI
BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV
FalI FspI FspAI HaeII Hin4I HincII HindIII HpaI
KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI
NcoI NdeI NgoMIV NheI NotI NspI PasI PciI
PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI
PstI PvuI PvuII SacI SalI SanDI SapI SbfI
ScaI SexAI SfcI SfiI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AscI AvrII BaeI BbeI BfrBI BstZ17I FseI KasI
NarI NruI NsiI PacI PmlI PspXI PsrI RsrII
SacII SfoI SrfI SwaI