Restriction map for
pFN21AB5522
(Circular) (Six-base) MAPSORT of: pFN21AB5522.seq Check: 7037 from: 1 to: 5851
pFN21AB5522 5851 bp
With 182 enzymes: SgfI *
February 27, 2009 14:34 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3661 278
Size: 53 83 186 936 2125 2468
Fragments arranged by size:
2468 2125 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3194 1937
Size: 1257 4594
Acc65I G'GTAC_C
Cuts at: 3172 3172
Size: 5851
AclI AA'CG_TT
Cuts at: 1563 3978 4351 1563
Size: 2415 373 3063
Fragments arranged by size:
3063 2415 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2860 3914 5298 5566 1632
Size: 1228 1054 1384 268 1917
Fragments arranged by size:
1917 1384 1228 1054 268
AfeI AGC'GCT
Cuts at: 2429 2429
Size: 5851
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5654
AflIII A'CryG_T
Cuts at: 1203 4750 5727 1203
Size: 3547 977 1327
Fragments arranged by size:
3547 1327 977
AgeI A'CCGG_T
Cuts at: 4682 4682
Size: 5851
AhdI GACnn_n'nnGTC
Cuts at: 4580 4580
Size: 5851
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5851
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5851
AlwNI CAG_nnn'CTG
Cuts at: 2502 3594 5166 2502
Size: 1092 1572 3187
Fragments arranged by size:
3187 1572 1092
ApaI G_GGCC'C
Cuts at: 2013 2013
Size: 5851
ApaLI G'TGCA_C
Cuts at: 3908 5064 3908
Size: 1156 4695
ApoI r'AATT_y
Cuts at: 1493 2228 3020 3050 3160 3386 3538 5647 1493
Size: 735 792 30 110 226 152 2109 1697
Fragments arranged by size:
2109 1697 792 735 226 152 110 30
AseI AT'TA_AT
Cuts at: 160 1032 4405 160
Size: 872 3373 1606
Fragments arranged by size:
3373 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5851
AvaI C'yCGr_G
Cuts at: 1870 1943 2998 3176 1870
Size: 73 1055 178 4545
Fragments arranged by size:
4545 1055 178 73
BamHI G'GATC_C
Cuts at: 3181 3548 3181
Size: 367 5484
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2812 2918 3172 4627 618
Size: 325 217 627 1025 106 254 1455 1842
Fragments arranged by size:
1842 1455 1025 627 325 254 217 106
BanII G_rGCy'C
Cuts at: 1391 2013 3170 1391
Size: 622 1157 4072
Fragments arranged by size:
4072 1157 622
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2623 2788 2864 3117 928
Size: 971 724 165 76 253 3662
Fragments arranged by size:
3662 971 724 253 165 76
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4042 4076 1903
Size: 34 2105 34 3678
Fragments arranged by size:
3678 2105 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3743 4953 3743
Size: 1210 4641
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5851
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2810 4462 136
Size: 107 122 71 631 605 1138 1652 1525
Fragments arranged by size:
1652 1525 1138 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5846 5846
Size: 5851
BlpI GC'TnA_GC
Cuts at: 3268 3268
Size: 5851
Bme1580I G_kGCm'C
Cuts at: 1790 2013 2815 2999 3912 5068 1790
Size: 223 802 184 913 1156 2573
Fragments arranged by size:
2573 1156 913 802 223 184
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5851
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2344 3582 4540
Size: 416 53 83 399 24 927 1238 958
Cuts at: 4540 442
Size: 1753
Fragments arranged by size:
1753 1238 958 927 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5851
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2207 4511 1239
Size: 968 2304 2579
Fragments arranged by size:
2579 2304 968
Bpu10I CC'TnA_GC
Cuts at: 2789 2789
Size: 5851
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2753 3976 4841 5139 2753
Size: 1223 865 298 3465
Fragments arranged by size:
3465 1223 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2056 3107 4514 882
Size: 617 557 1051 1407 2219
Fragments arranged by size:
2219 1407 1051 617 557
BsaAI yAC'GTr
Cuts at: 493 1204 3642 493
Size: 711 2438 2702
Fragments arranged by size:
2702 2438 711
BsaBI GATnn'nnATC
Cuts at: 5845 5845
Size: 5851
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3658 4040 4675 275
Size: 53 83 186 936 2125 382 635 1451
Fragments arranged by size:
2125 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 2973 4284 4682 4956 5103 5514 2973
Size: 1311 398 274 147 411 3310
Fragments arranged by size:
3310 1311 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 3062 3092 1178
Size: 30 165 30 1659 30 3937
Fragments arranged by size:
3937 1659 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2399 1186
Size: 1213 4638
BseYI C'CCAG_C
Cuts at: 2505 2808 5054 5550 2505
Size: 303 2246 496 2806
Fragments arranged by size:
2806 2246 496 303
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2605 1163
Size: 573 869 4409
Fragments arranged by size:
4409 869 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3301 4062 5090 1540
Size: 275 186 1300 761 1028 2301
Fragments arranged by size:
2301 1300 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2910 3912 3997 5068 2910
Size: 1002 85 1071 3693
Fragments arranged by size:
3693 1071 1002 85
BsmI GAATG_Cn'
Cuts at: 3362 3455 3362
Size: 93 5758
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2013 2815 2910 2999 3170 3912 3997
Size: 399 223 802 95 89 171 742 85
Cuts at: 3997 5068 1391
Size: 1071 2174
Fragments arranged by size:
2174 1071 802 742 399 223 171 95
89 85
BspEI T'CCGG_A
Cuts at: 2973 5514 2973
Size: 2541 3310
BspHI T'CATG_A
Cuts at: 3740 5470 3740
Size: 1730 4121
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2316 3206 844
Size: 1472 890 3489
Fragments arranged by size:
3489 1472 890
BsrBI CCG'CTC
Cuts at: 1394 1823 2593 2818 2882 3003 3738 1394
Size: 429 770 225 64 121 735 3507
Fragments arranged by size:
3507 770 735 429 225 121 64
BsrDI GCAATG_nn'
Cuts at: 65 1248 4346 4520 65
Size: 1183 3098 174 1396
Fragments arranged by size:
3098 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 3041 4495 4682 1700
Size: 110 1231 1454 187 2869
Fragments arranged by size:
2869 1454 1231 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5851
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5851
BssSI C'ACGA_G
Cuts at: 3911 4923 3911
Size: 1012 4839
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5851
BstBI TT'CG_AA
Cuts at: 4655 4655
Size: 5851
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5851
BstYI r'GATC_y
Cuts at: 1972 3181 3548 3933 3950 5402 5488 5500 5846
Size: 1209 367 385 17 1452 86 12 346
Cuts at: 5846 1972
Size: 1977
Fragments arranged by size:
1977 1452 1209 385 367 346 86 17
12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2625 513
Size: 552 939 621 3739
Fragments arranged by size:
3739 939 621 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2401 2785 3022 487
Size: 683 56 1175 384 237 3316
Fragments arranged by size:
3316 1175 683 384 237 56
BtsI GCAGTG_nn'
Cuts at: 3367 4161 4181 3367
Size: 794 20 5037
Fragments arranged by size:
5037 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5851
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5816
DraI TTT'AAA
Cuts at: 3155 3155
Size: 5851
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 3121 4858 809
Size: 507 1805 1737 1802
Fragments arranged by size:
1805 1802 1737 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3298 4187 5816
Size: 54 1425 50 275 18 1468 889 1629
Cuts at: 5816 8
Size: 43
Fragments arranged by size:
1629 1468 1425 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 3298 1537
Size: 275 1486 4090
Fragments arranged by size:
4090 1486 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2489 2621 2825 2879 3148 3781 1353
Size: 1136 132 204 54 269 633 3423
Fragments arranged by size:
3423 1136 633 269 204 132 54
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2468 4438 4812 4958 387
Size: 1064 138 879 1970 374 146 1280
Fragments arranged by size:
1970 1280 1064 879 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2207 2860 3914 4511 5298 5566 1239
Size: 393 575 653 1054 597 787 268 1524
Fragments arranged by size:
1524 1054 787 653 597 575 393 268
EcoNI CCTnn'n_nnAGG
Cuts at: 2686 2686
Size: 5851
EcoO109I rG'GnC_Cy
Cuts at: 2009 2009
Size: 5851
EcoRI G'AATT_C
Cuts at: 3050 3160 3538 3050
Size: 110 378 5363
Fragments arranged by size:
5363 378 110
EcoRV GAT'ATC
Cuts at: 2074 2074
Size: 5851
FspI TGC'GCA
Cuts at: 4357 4357
Size: 5851
HaeII r_GCGC'y
Cuts at: 1678 2431 2502 2765 4998 1678
Size: 753 71 263 2233 2531
Fragments arranged by size:
2531 2233 753 263 71
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 3021 3053 3062 3094 4492
Size: 32 135 32 1449 32 9 32 1398
Cuts at: 4492 4524 4566 4598 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1449 1398 135 42 32 32 32
32 32 32 9
HincII GTy'rAC
Cuts at: 669 1657 1938 2065 3139 3195 3441 669
Size: 988 281 127 1074 56 246 3079
Fragments arranged by size:
3079 1074 988 281 246 127 56
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5851
HpaI GTT'AAC
Cuts at: 3441 3441
Size: 5851
KpnI G_GTAC'C
Cuts at: 3176 3176
Size: 5851
MfeI C'AATT_G
Cuts at: 3450 3450
Size: 5851
MluI A'CGCG_T
Cuts at: 5727 5727
Size: 5851
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4965 5149 1305
Size: 3660 184 2007
Fragments arranged by size:
3660 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4029
Fragments arranged by size:
4029 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3809 4168 4327 518
Size: 648 2643 359 159 2042
Fragments arranged by size:
2643 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2026 2574 3267 3944 5092 5337 1606
Size: 420 548 693 677 1148 245 2120
Fragments arranged by size:
2120 1148 693 677 548 420 245
NaeI GCC'GGC
Cuts at: 3043 3043
Size: 5851
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4360
Fragments arranged by size:
4360 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5851
NgoMIV G'CCGG_C
Cuts at: 3041 3041
Size: 5851
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5851
NotI GC'GGCC_GC
Cuts at: 3298 3298
Size: 5851
NspI r_CATG'y
Cuts at: 1132 1229 3209 4754 1132
Size: 97 1980 1545 2229
Fragments arranged by size:
2229 1980 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5851
PciI A'CATG_T
Cuts at: 4750 4750
Size: 5851
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5851
PfoI T'CCnGG_A
Cuts at: 2184 2184
Size: 5851
PmeI GTTT'AAAC
Cuts at: 3155 3155
Size: 5851
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3920 3952 5433 5465 3920
Size: 32 1481 32 4306
Fragments arranged by size:
4306 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5851
PsiI TTA'TAA
Cuts at: 3421 3421
Size: 5851
PspOMI G'GGCC_C
Cuts at: 2009 2009
Size: 5851
PstI C_TGCA'G
Cuts at: 830 2672 3203 830
Size: 1842 531 3478
Fragments arranged by size:
3478 1842 531
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5851
PvuII CAG'CTG
Cuts at: 2574 2574
Size: 5851
SalI G'TCGA_C
Cuts at: 1936 3193 1936
Size: 1257 4594
SapI GCTCTTCn'nnn_
Cuts at: 2825 2825
Size: 5851
SbfI CC_TGCA'GG
Cuts at: 3203 3203
Size: 5851
ScaI AGT'ACT
Cuts at: 2888 4099 5811 2888
Size: 1211 1712 2928
Fragments arranged by size:
2928 1712 1211
SfcI C'TryA_G
Cuts at: 826 1045 2668 3199 4334 5015 5206 826
Size: 219 1623 531 1135 681 191 1471
Fragments arranged by size:
1623 1471 1135 681 531 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5851
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5851
SmaI CCC'GGG
Cuts at: 1872 3178 1872
Size: 1306 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2768 3955 4856 5118 820
Size: 197 926 825 1187 901 262 1553
Fragments arranged by size:
1553 1187 926 901 825 262 197
SnaBI TAC'GTA
Cuts at: 493 3642 493
Size: 3149 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5851
SphI G_CATG'C
Cuts at: 1132 3209 1132
Size: 2077 3774
SspI AAT'ATT
Cuts at: 5 52 3775 5
Size: 47 3723 2081
Fragments arranged by size:
3723 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5851
StyI C'CwwG_G
Cuts at: 513 1065 2004 2682 2730 3290 513
Size: 552 939 678 48 560 3074
Fragments arranged by size:
3074 939 678 560 552 48
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2038 2516 3878 3895 4048 4233 4678
Size: 1255 458 478 1362 17 153 185 445
Cuts at: 4678 325
Size: 1498
Fragments arranged by size:
1498 1362 1255 478 458 445 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2244 2664 2886
Size: 274 80 33 51 1443 267 420 222
Cuts at: 2886 4097 5809 96
Size: 1211 1712 138
Fragments arranged by size:
1712 1443 1211 420 274 267 222 138
80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5811 5843 5811
Size: 32 5819
Tth111I GACn'n_nGTC
Cuts at: 1316 2060 1316
Size: 744 5107
XbaI T'CTAG_A
Cuts at: 3187 3187
Size: 5851
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2023 2023
Size: 5851
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5851
XmaI C'CCGG_G
Cuts at: 1870 3176 1870
Size: 1306 4545
XmnI GAAnn'nnTTC
Cuts at: 3980 3980
Size: 5851
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3659 276
Size: 53 83 186 936 2125 2468
Fragments arranged by size:
2468 2125 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI
BtsI ClaI CspCI DraI DrdI EaeI EagI EarI
EciI Eco57MI EcoNI EcoO109I EcoRI EcoRV FspI HaeII
Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI
MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI
NotI NspI PasI PciI PflMI PfoI PmeI PpiI
PshAI PsiI PspOMI PstI PvuI PvuII SalI SapI
SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AloI AscI AvrII BaeI BbeI BbvCI BfrBI
BplI BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoICRI
FalI FseI FspAI KasI NarI NruI NsiI PacI
PmlI PpuMI PspXI PsrI RsrII SacI SacII SanDI
SexAI SfiI SfoI SrfI SwaI