Restriction map for
pFN21AB5520
(Circular) (Six-base) MAPSORT of: pFN21AB5520.seq Check: 7349 from: 1 to: 5707
pFN21AB5520 5707 bp
With 182 enzymes: SgfI *
January 16, 2009 15:25 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2891 2891
Size: 5707
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3517 278
Size: 53 83 186 936 1981 2468
Fragments arranged by size:
2468 1981 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3050 1937
Size: 1113 4594
Acc65I G'GTAC_C
Cuts at: 3028 3028
Size: 5707
AclI AA'CG_TT
Cuts at: 1563 3834 4207 1563
Size: 2271 373 3063
Fragments arranged by size:
3063 2271 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2108 2287 3770 5154 5422 1632
Size: 476 179 1483 1384 268 1917
Fragments arranged by size:
1917 1483 1384 476 268 179
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5510
AflIII A'CryG_T
Cuts at: 1203 4606 5583 1203
Size: 3403 977 1327
Fragments arranged by size:
3403 1327 977
AgeI A'CCGG_T
Cuts at: 4538 4538
Size: 5707
AhdI GACnn_n'nnGTC
Cuts at: 4436 4436
Size: 5707
AleI CACnn'nnGTG
Cuts at: 1166 2089 1166
Size: 923 4784
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2058 1184
Size: 874 4833
AlwNI CAG_nnn'CTG
Cuts at: 3450 5022 3450
Size: 1572 4135
ApaI G_GGCC'C
Cuts at: 2877 2877
Size: 5707
ApaLI G'TGCA_C
Cuts at: 3764 4920 3764
Size: 1156 4551
ApoI r'AATT_y
Cuts at: 1493 2138 2267 2309 3016 3242 3394 5503 1493
Size: 645 129 42 707 226 152 2109 1697
Fragments arranged by size:
2109 1697 707 645 226 152 129 42
AseI AT'TA_AT
Cuts at: 160 1032 4261 160
Size: 872 3229 1606
Fragments arranged by size:
3229 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5707
AvaI C'yCGr_G
Cuts at: 1870 1943 2051 2391 2862 2870 3032 1870
Size: 73 108 340 471 8 162 4545
Fragments arranged by size:
4545 471 340 162 108 73 8
BamHI G'GATC_C
Cuts at: 3037 3404 3037
Size: 367 5340
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2866 2954 3028 4483 618
Size: 325 217 627 1079 88 74 1455 1842
Fragments arranged by size:
1842 1455 1079 627 325 217 88 74
BanII G_rGCy'C
Cuts at: 1391 2877 3026 1391
Size: 1486 149 4072
Fragments arranged by size:
4072 1486 149
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2293 2358 928
Size: 971 394 65 4277
Fragments arranged by size:
4277 971 394 65
BbvCI CC'TCA_GC
Cuts at: 2721 2721
Size: 5707
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2693 2727 3898 3932 1903
Size: 34 756 34 1171 34 3678
Fragments arranged by size:
3678 1171 756 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3599 4809 3599
Size: 1210 4497
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5707
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2794 4318 136
Size: 107 122 71 631 605 1122 1524 1525
Fragments arranged by size:
1525 1524 1122 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5702 5702
Size: 5707
BlpI GC'TnA_GC
Cuts at: 3124 3124
Size: 5707
Bme1580I G_kGCm'C
Cuts at: 1790 2869 2877 3768 4924 1790
Size: 1079 8 891 1156 2573
Fragments arranged by size:
2573 1156 1079 891 8
BmgBI CAC'GTC
Cuts at: 1318 2538 1318
Size: 1220 4487
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3438 4396 442
Size: 416 53 83 399 24 2021 958 1753
Fragments arranged by size:
2021 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5707
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 4367 1239
Size: 3128 2579
Bpu10I CC'TnA_GC
Cuts at: 2721 2721
Size: 5707
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3832 4697 4995 3832
Size: 865 298 4544
Fragments arranged by size:
4544 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4370 882
Size: 617 2871 2219
Fragments arranged by size:
2871 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3498 493
Size: 711 2294 2702
Fragments arranged by size:
2702 2294 711
BsaBI GATnn'nnATC
Cuts at: 2106 5701 2106
Size: 3595 2112
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3514 3896 4531 275
Size: 53 83 186 936 1981 382 635 1451
Fragments arranged by size:
1981 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4140 4538 4812 4959 5370 4140
Size: 398 274 147 411 4477
Fragments arranged by size:
4477 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5482
Fragments arranged by size:
5482 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2247 2250 2738 2852 2855 1186
Size: 1061 3 488 114 3 4038
Fragments arranged by size:
4038 1061 488 114 3 3
BseYI C'CCAG_C
Cuts at: 2760 4910 5406 2760
Size: 2150 496 3061
Fragments arranged by size:
3061 2150 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2054 1163
Size: 573 318 4816
Fragments arranged by size:
4816 573 318
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3157 3918 4946 1540
Size: 275 186 1156 761 1028 2301
Fragments arranged by size:
2301 1156 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3768 3853 4924 3768
Size: 85 1071 4551
Fragments arranged by size:
4551 1071 85
BsmI GAATG_Cn'
Cuts at: 3218 3311 3218
Size: 93 5614
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2869 2877 3026 3768 3853 4924 1391
Size: 399 1079 8 149 742 85 1071 2174
Fragments arranged by size:
2174 1079 1071 742 399 149 85 8
BspEI T'CCGG_A
Cuts at: 5370 5370
Size: 5707
BspHI T'CATG_A
Cuts at: 3596 5326 3596
Size: 1730 3977
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2891 3062 844
Size: 2047 171 3489
Fragments arranged by size:
3489 2047 171
BsrBI CCG'CTC
Cuts at: 1394 1823 3594 1394
Size: 429 1771 3507
Fragments arranged by size:
3507 1771 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4202 4376 65
Size: 1183 2954 174 1396
Fragments arranged by size:
2954 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2768 4351 4538 1700
Size: 110 958 1583 187 2869
Fragments arranged by size:
2869 1583 958 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5707
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5707
BssSI C'ACGA_G
Cuts at: 3767 4779 3767
Size: 1012 4695
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5707
BstBI TT'CG_AA
Cuts at: 4511 4511
Size: 5707
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5707
BstYI r'GATC_y
Cuts at: 1972 3037 3404 3789 3806 5258 5344 5356 5702
Size: 1065 367 385 17 1452 86 12 346
Cuts at: 5702 1972
Size: 1977
Fragments arranged by size:
1977 1452 1065 385 367 346 86 17
12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2922 513
Size: 552 939 918 3298
Fragments arranged by size:
3298 939 918 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4968
Fragments arranged by size:
4968 683 56
BtsI GCAGTG_nn'
Cuts at: 3223 4017 4037 3223
Size: 794 20 4893
Fragments arranged by size:
4893 794 20
ClaI AT'CG_AT
Cuts at: 1554 2573 1554
Size: 1019 4688
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2524 2559 529
Size: 35 1960 35 3677
Fragments arranged by size:
3677 1960 35 35
DraI TTT'AAA
Cuts at: 3011 3011
Size: 5707
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4714 809
Size: 507 3398 1802
Fragments arranged by size:
3398 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2668 2700 3154
Size: 54 1425 50 275 18 838 32 454
Cuts at: 3154 4043 5672 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 889 838 454 275 54 50
43 32 18
EagI C'GGCC_G
Cuts at: 1537 1812 3154 1537
Size: 275 1342 4090
Fragments arranged by size:
4090 1342 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2536 3637 1353
Size: 1183 1101 3423
Fragments arranged by size:
3423 1183 1101
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2765 4294 4668 4814 387
Size: 1064 138 1176 1529 374 146 1280
Fragments arranged by size:
1529 1280 1176 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2108 2287 3770 4367 5154 5422 1239
Size: 393 476 179 1483 597 787 268 1524
Fragments arranged by size:
1524 1483 787 597 476 393 268 179
EcoNI CCTnn'n_nnAGG
Cuts at: 2720 2720
Size: 5707
EcoO109I rG'GnC_Cy
Cuts at: 2326 2387 2515 2874 2881 2326
Size: 61 128 359 7 5152
Fragments arranged by size:
5152 359 128 61 7
EcoRI G'AATT_C
Cuts at: 3016 3394 3016
Size: 378 5329
EcoRV GAT'ATC
Cuts at: 2577 2577
Size: 5707
FspI TGC'GCA
Cuts at: 4213 4213
Size: 5707
HaeII r_GCGC'y
Cuts at: 1678 4854 1678
Size: 3176 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 4348 4380 4422 4454 1373
Size: 32 135 32 2776 32 42 32 2626
Fragments arranged by size:
2776 2626 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3051 3297 669
Size: 988 281 1113 246 3079
Fragments arranged by size:
3079 1113 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5707
HpaI GTT'AAC
Cuts at: 3297 3297
Size: 5707
KpnI G_GTAC'C
Cuts at: 3032 3032
Size: 5707
MfeI C'AATT_G
Cuts at: 3306 3306
Size: 5707
MluI A'CGCG_T
Cuts at: 5583 5583
Size: 5707
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2154 4821 5005 1305
Size: 849 2667 184 2007
Fragments arranged by size:
2667 2007 849 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2670 10
Size: 54 1425 343 838 3047
Fragments arranged by size:
3047 1425 838 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2089 3665 4024 4183 518
Size: 648 923 1576 359 159 2042
Fragments arranged by size:
2042 1576 923 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 3123 3800 4948 5193 1606
Size: 1517 677 1148 245 2120
Fragments arranged by size:
2120 1517 1148 677 245
NaeI GCC'GGC
Cuts at: 2770 2770
Size: 5707
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4216
Fragments arranged by size:
4216 939 552
NdeI CA'TA_TG
Cuts at: 387 2161 387
Size: 1774 3933
NgoMIV G'CCGG_C
Cuts at: 2768 2768
Size: 5707
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5707
NotI GC'GGCC_GC
Cuts at: 3154 3154
Size: 5707
NspI r_CATG'y
Cuts at: 1132 1229 2045 2734 3065 4610 1132
Size: 97 816 689 331 1545 2229
Fragments arranged by size:
2229 1545 816 689 331 97
PasI CC'CwG_GG
Cuts at: 1348 2877 1348
Size: 1529 4178
PciI A'CATG_T
Cuts at: 4606 4606
Size: 5707
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5707
PmeI GTTT'AAAC
Cuts at: 3011 3011
Size: 5707
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3776 3808 5289 5321 3776
Size: 32 1481 32 4162
Fragments arranged by size:
4162 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2326 2387 2881 2326
Size: 61 494 5152
Fragments arranged by size:
5152 494 61
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5707
PsiI TTA'TAA
Cuts at: 3277 3277
Size: 5707
PspOMI G'GGCC_C
Cuts at: 2873 2873
Size: 5707
PstI C_TGCA'G
Cuts at: 830 3059 830
Size: 2229 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5707
SalI G'TCGA_C
Cuts at: 1936 3049 1936
Size: 1113 4594
SanDI GG'GwC_CC
Cuts at: 2326 2387 2881 2326
Size: 61 494 5152
Fragments arranged by size:
5152 494 61
SbfI CC_TGCA'GG
Cuts at: 3059 3059
Size: 5707
ScaI AGT'ACT
Cuts at: 3955 5667 3955
Size: 1712 3995
SfcI C'TryA_G
Cuts at: 826 1045 3055 4190 4871 5062 826
Size: 219 2010 1135 681 191 1471
Fragments arranged by size:
2010 1471 1135 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5707
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5707
SmaI CCC'GGG
Cuts at: 1872 2872 3034 1872
Size: 1000 162 4545
Fragments arranged by size:
4545 1000 162
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3811 4712 4974 820
Size: 197 926 1868 901 262 1553
Fragments arranged by size:
1868 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3498 493
Size: 3005 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5707
SphI G_CATG'C
Cuts at: 1132 2734 3065 1132
Size: 1602 331 3774
Fragments arranged by size:
3774 1602 331
SspI AAT'ATT
Cuts at: 5 52 3631 5
Size: 47 3579 2081
Fragments arranged by size:
3579 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5707
StyI C'CwwG_G
Cuts at: 513 1065 2004 3146 513
Size: 552 939 1142 3074
Fragments arranged by size:
3074 1142 939 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2871 2942 3734 3751 3904 4089 4534
Size: 1255 1291 71 792 17 153 185 445
Cuts at: 4534 325
Size: 1498
Fragments arranged by size:
1498 1291 1255 792 445 185 153 71
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3953 5665 96
Size: 274 80 33 51 1443 1976 1712 138
Fragments arranged by size:
1976 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5667 5699 5667
Size: 32 5675
Tth111I GACn'n_nGTC
Cuts at: 1316 2416 1316
Size: 1100 4607
XbaI T'CTAG_A
Cuts at: 3043 3043
Size: 5707
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5707
XmaI C'CCGG_G
Cuts at: 1870 2870 3032 1870
Size: 1000 162 4545
Fragments arranged by size:
4545 1000 162
XmnI GAAnn'nnTTC
Cuts at: 3836 3836
Size: 5707
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3515 276
Size: 53 83 186 936 1981 2468
Fragments arranged by size:
2468 1981 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI
BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI
EarI EciI Eco57MI EcoNI EcoO109I EcoRI EcoRV FspI
HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI
MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV
NheI NotI NspI PasI PciI PflMI PmeI PpiI
PpuMI PshAI PsiI PspOMI PstI PvuI SalI SanDI
SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI AscI AvrII BaeI BbeI BfrBI BplI
BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoICRI FalI
FseI FspAI KasI NarI NruI NsiI PacI PfoI
PmlI PspXI PsrI PvuII RsrII SacI SacII SapI
SexAI SfiI SfoI SrfI SwaI XcmI