Restriction map for
pFN21AB5511
(Circular) (Six-base) MAPSORT of: pFN21AB5511.seq Check: 2107 from: 1 to: 6886
pFN21AB5511 6886 bp
With 182 enzymes: SgfI *
February 27, 2009 14:35 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2210 3558 2210
Size: 1348 5538
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 2216 4696 278
Size: 53 83 186 936 680 2480 2468
Fragments arranged by size:
2480 2468 936 680 186 83 53
AccI GT'mk_AC
Cuts at: 1937 4229 1937
Size: 2292 4594
Acc65I G'GTAC_C
Cuts at: 4207 4207
Size: 6886
AclI AA'CG_TT
Cuts at: 1563 5013 5386 1563
Size: 3450 373 3063
Fragments arranged by size:
3450 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2267 2366 3124 4949 6333 6601 1632
Size: 635 99 758 1825 1384 268 1917
Fragments arranged by size:
1917 1825 1384 758 635 268 99
AfeI AGC'GCT
Cuts at: 2141 2141
Size: 6886
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6689
AflIII A'CryG_T
Cuts at: 1203 5785 6762 1203
Size: 4582 977 1327
Fragments arranged by size:
4582 1327 977
AgeI A'CCGG_T
Cuts at: 5717 5717
Size: 6886
AhdI GACnn_n'nnGTC
Cuts at: 5615 5615
Size: 6886
AleI CACnn'nnGTG
Cuts at: 1166 3692 1166
Size: 2526 4360
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 3124 3404 1184
Size: 1940 280 4666
Fragments arranged by size:
4666 1940 280
AlwNI CAG_nnn'CTG
Cuts at: 2632 4629 6201 2632
Size: 1997 1572 3317
Fragments arranged by size:
3317 1997 1572
ApaI G_GGCC'C
Cuts at: 2334 3059 3910 2334
Size: 725 851 5310
Fragments arranged by size:
5310 851 725
ApaLI G'TGCA_C
Cuts at: 4943 6099 4943
Size: 1156 5730
ApoI r'AATT_y
Cuts at: 1493 3845 4195 4421 4573 6682 1493
Size: 2352 350 226 152 2109 1697
Fragments arranged by size:
2352 2109 1697 350 226 152
AseI AT'TA_AT
Cuts at: 160 1032 5440 160
Size: 872 4408 1606
Fragments arranged by size:
4408 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6886
AvaI C'yCGr_G
Cuts at: 1870 1943 2334 3584 4211 1870
Size: 73 391 1250 627 4545
Fragments arranged by size:
4545 1250 627 391 73
AvrII C'CTAG_G
Cuts at: 2182 2182
Size: 6886
BamHI G'GATC_C
Cuts at: 4216 4583 4216
Size: 367 6519
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 4151 4207 5662 618
Size: 325 217 627 2364 56 1455 1842
Fragments arranged by size:
2364 1842 1455 627 325 217 56
BanII G_rGCy'C
Cuts at: 1391 2334 2343 2397 2465 3059 3174 3268 3910
Size: 943 9 54 68 594 115 94 642
Cuts at: 3910 4078 4137 4205 1391
Size: 168 59 68 4072
Fragments arranged by size:
4072 943 642 594 168 115 94 68
68 59 54 9
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2371 928
Size: 971 472 5443
Fragments arranged by size:
5443 971 472
BbvCI CC'TCA_GC
Cuts at: 2388 2495 2388
Size: 107 6779
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 5077 5111 1903
Size: 34 50 34 3056 34 3678
Fragments arranged by size:
3678 3056 50 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2439 4778 5988 2439
Size: 2339 1210 3337
Fragments arranged by size:
3337 2339 1210
BclI T'GATC_A
Cuts at: 1548 3372 1548
Size: 1824 5062
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2838 5497 136
Size: 107 122 71 631 605 1166 2659 1525
Fragments arranged by size:
2659 1525 1166 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2559 3412 6881 2559
Size: 853 3469 2564
Fragments arranged by size:
3469 2564 853
BlpI GC'TnA_GC
Cuts at: 2143 4303 2143
Size: 2160 4726
Bme1580I G_kGCm'C
Cuts at: 1790 2334 3059 3351 3388 3910 4154 4947 6103
Size: 544 725 292 37 522 244 793 1156
Cuts at: 6103 1790
Size: 2573
Fragments arranged by size:
2573 1156 793 725 544 522 292 244
37
BmgBI CAC'GTC
Cuts at: 1318 2314 3157 1318
Size: 996 843 5047
Fragments arranged by size:
5047 996 843
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3904 4617 5575
Size: 416 53 83 399 24 2487 713 958
Cuts at: 5575 442
Size: 1753
Fragments arranged by size:
2487 1753 958 713 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6886
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3973 3973
Size: 6886
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2279 2438 2487 3875 5546 1239
Size: 1040 159 49 1388 1671 2579
Fragments arranged by size:
2579 1671 1388 1040 159 49
Bpu10I CC'TnA_GC
Cuts at: 2388 2495 2997 2388
Size: 107 502 6277
Fragments arranged by size:
6277 502 107
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2645 2819 5011 5876 6174 2645
Size: 174 2192 865 298 3357
Fragments arranged by size:
3357 2192 865 298 174
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2108 2414 3747 5549 882
Size: 617 609 306 1333 1802 2219
Fragments arranged by size:
2219 1802 1333 617 609 306
BsaAI yAC'GTr
Cuts at: 493 1204 3307 4677 493
Size: 711 2103 1370 2702
Fragments arranged by size:
2702 2103 1370 711
BsaBI GATnn'nnATC
Cuts at: 2594 6880 2594
Size: 4286 2600
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2067 2213 3653 4693
Size: 53 83 186 936 534 146 1440 1040
Cuts at: 4693 5075 5710 275
Size: 382 635 1451
Fragments arranged by size:
1451 1440 1040 936 635 534 382 186
146 83 53
BsaWI w'CCGG_w
Cuts at: 2431 5319 5717 5991 6138 6549 2431
Size: 2888 398 274 147 411 2768
Fragments arranged by size:
2888 2768 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2411 2441 1178
Size: 30 165 30 1008 30 5623
Fragments arranged by size:
5623 1008 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2405 3731 1186
Size: 1219 1326 4341
Fragments arranged by size:
4341 1326 1219
BseYI C'CCAG_C
Cuts at: 2032 6089 6585 2032
Size: 4057 496 2333
Fragments arranged by size:
4057 2333 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2788 3112 3294 3338 3536 1163
Size: 573 1052 324 182 44 198 4513
Fragments arranged by size:
4513 1052 573 324 198 182 44
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4336 5097 6125 1540
Size: 275 186 2335 761 1028 2301
Fragments arranged by size:
2335 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2343 2397 2661 3729 4947 5032 6103 2343
Size: 54 264 1068 1218 85 1071 3126
Fragments arranged by size:
3126 1218 1071 1068 264 85 54
BsmI GAATG_Cn'
Cuts at: 3082 4397 4490 3082
Size: 1315 93 5478
Fragments arranged by size:
5478 1315 93
BsmBI CGTCTCn'nnnn_
Cuts at: 3644 3644
Size: 6886
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2334 2343 2397 2465 2661 3059 3174
Size: 399 544 9 54 68 196 398 115
Cuts at: 3174 3268 3351 3388 3729 3910 4078 4137 4154
Size: 94 83 37 341 181 168 59 17
Cuts at: 4154 4205 4947 5032 6103 1391
Size: 51 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 544 399 398 341 196
181 168 115 94 85 83 68 59
54 51 37 17 9
BspEI T'CCGG_A
Cuts at: 6549 6549
Size: 6886
BspHI T'CATG_A
Cuts at: 4775 6505 4775
Size: 1730 5156
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2210 3558 4241 844
Size: 1366 1348 683 3489
Fragments arranged by size:
3489 1366 1348 683
BsrBI CCG'CTC
Cuts at: 1394 1823 3832 4773 1394
Size: 429 2009 941 3507
Fragments arranged by size:
3507 2009 941 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3213 3861 5381 5555 65
Size: 1183 1965 648 1520 174 1396
Fragments arranged by size:
1965 1520 1396 1183 648 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 3111 3498 5530 5717 1700
Size: 110 1301 387 2032 187 2869
Fragments arranged by size:
2869 2032 1301 387 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6886
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6886
BssSI C'ACGA_G
Cuts at: 2978 4946 5958 2978
Size: 1968 1012 3906
Fragments arranged by size:
3906 1968 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6886
BstBI TT'CG_AA
Cuts at: 2793 5690 2793
Size: 2897 3989
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6886
BstXI CCAn_nnnn'nTGG
Cuts at: 2177 2869 3094 3631 2177
Size: 692 225 537 5432
Fragments arranged by size:
5432 692 537 225
BstYI r'GATC_y
Cuts at: 1972 2166 2281 2559 3412 4216 4583 4968 4985
Size: 194 115 278 853 804 367 385 17
Cuts at: 4985 6437 6523 6535 6881 1972
Size: 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 853 804 385 367 346 278
194 115 86 17 12
Bsu36I CC'TnA_GG
Cuts at: 3060 3060
Size: 6886
BtgI C'CryG_G
Cuts at: 513 1065 2725 2862 513
Size: 552 1660 137 4537
Fragments arranged by size:
4537 1660 552 137
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 3985 4147 487
Size: 683 56 2759 162 3226
Fragments arranged by size:
3226 2759 683 162 56
BtsI GCAGTG_nn'
Cuts at: 3388 4402 5196 5216 3388
Size: 1014 794 20 5058
Fragments arranged by size:
5058 1014 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6886
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2884 2919 529
Size: 35 2320 35 4496
Fragments arranged by size:
4496 2320 35 35
DraI TTT'AAA
Cuts at: 4190 4190
Size: 6886
DraIII CAC_nnn'GTG
Cuts at: 2811 2811
Size: 6886
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5893 809
Size: 507 4577 1802
Fragments arranged by size:
4577 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2049 2103 2508
Size: 54 1425 50 275 18 219 54 405
Cuts at: 2508 3511 3733 4333 5222 6851 8
Size: 1003 222 600 889 1629 43
Fragments arranged by size:
1629 1425 1003 889 600 405 275 222
219 54 54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 4333 1537
Size: 275 2521 4090
Fragments arranged by size:
4090 2521 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2300 2573 3000 3422 4816 1353
Size: 947 273 427 422 1394 3423
Fragments arranged by size:
3423 1394 947 427 422 273
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5473 5847 5993 387
Size: 1064 138 3884 374 146 1280
Fragments arranged by size:
3884 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2341 2395 2341
Size: 54 6832
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2267 2279 2366 2438 2487 3124 3875
Size: 393 635 12 87 72 49 637 751
Cuts at: 3875 4949 5546 6333 6601 1239
Size: 1074 597 787 268 1524
Fragments arranged by size:
1524 1074 787 751 637 635 597 393
268 87 72 49 12
EcoNI CCTnn'n_nnAGG
Cuts at: 2186 3566 2186
Size: 1380 5506
EcoO109I rG'GnC_Cy
Cuts at: 2330 2331 3056 3906 2330
Size: 1 725 850 5310
Fragments arranged by size:
5310 850 725 1
EcoRI G'AATT_C
Cuts at: 4195 4573 4195
Size: 378 6508
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2581 2581
Size: 6886
FspI TGC'GCA
Cuts at: 2828 5392 2828
Size: 2564 4322
HaeII r_GCGC'y
Cuts at: 1678 2143 2177 6033 1678
Size: 465 34 3856 2531
Fragments arranged by size:
3856 2531 465 34
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2413 2445 2460 2492 2952
Size: 32 135 32 841 32 15 32 460
Cuts at: 2952 2984 3023 3055 3389 3421 5527 5559 5601
Size: 32 39 32 334 32 2106 32 42
Cuts at: 5601 5633 1373
Size: 32 2626
Fragments arranged by size:
2626 2106 841 460 334 135 42 39
32 32 32 32 32 32 32 32
32 15
HincII GTy'rAC
Cuts at: 669 1657 1938 4230 4476 669
Size: 988 281 2292 246 3079
Fragments arranged by size:
3079 2292 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6886
HpaI GTT'AAC
Cuts at: 4476 4476
Size: 6886
KpnI G_GTAC'C
Cuts at: 4211 4211
Size: 6886
MfeI C'AATT_G
Cuts at: 4485 4485
Size: 6886
MluI A'CGCG_T
Cuts at: 6762 6762
Size: 6886
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3220 6000 6184 1305
Size: 1915 2780 184 2007
Fragments arranged by size:
2780 2007 1915 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2105 3513 3735 10
Size: 54 1425 343 273 1408 222 3161
Fragments arranged by size:
3161 1425 1408 343 273 222 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3692 3809 3975 4125 4844 5203 5362
Size: 648 2526 117 166 150 719 359 159
Cuts at: 5362 518
Size: 2042
Fragments arranged by size:
2526 2042 719 648 359 166 159 150
117
MspA1I CmG'CkG
Cuts at: 1606 2545 2613 2923 3126 3223 4302 4979 6127
Size: 939 68 310 203 97 1079 677 1148
Cuts at: 6127 6372 1606
Size: 245 2120
Fragments arranged by size:
2120 1148 1079 939 677 310 245 203
97 68
NaeI GCC'GGC
Cuts at: 3113 3113
Size: 6886
NcoI C'CATG_G
Cuts at: 513 1065 2725 513
Size: 552 1660 4674
Fragments arranged by size:
4674 1660 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6886
NgoMIV G'CCGG_C
Cuts at: 3111 3111
Size: 6886
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6886
NotI GC'GGCC_GC
Cuts at: 4333 4333
Size: 6886
NspI r_CATG'y
Cuts at: 1132 1229 2959 4244 5789 1132
Size: 97 1730 1285 1545 2229
Fragments arranged by size:
2229 1730 1545 1285 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6886
PciI A'CATG_T
Cuts at: 5785 5785
Size: 6886
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2632 1108
Size: 1524 5362
PmeI GTTT'AAAC
Cuts at: 4190 4190
Size: 6886
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4955 4987 6468 6500 4955
Size: 32 1481 32 5341
Fragments arranged by size:
5341 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6886
PsiI TTA'TAA
Cuts at: 4456 4456
Size: 6886
PspOMI G'GGCC_C
Cuts at: 2330 3055 3906 2330
Size: 725 851 5310
Fragments arranged by size:
5310 851 725
PstI C_TGCA'G
Cuts at: 830 2751 3405 4238 830
Size: 1921 654 833 3478
Fragments arranged by size:
3478 1921 833 654
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6886
PvuII CAG'CTG
Cuts at: 2545 3126 2545
Size: 581 6305
SacI G_AGCT'C
Cuts at: 2343 2397 2343
Size: 54 6832
SalI G'TCGA_C
Cuts at: 1936 4228 1936
Size: 2292 4594
SbfI CC_TGCA'GG
Cuts at: 4238 4238
Size: 6886
ScaI AGT'ACT
Cuts at: 5134 6846 5134
Size: 1712 5174
SfcI C'TryA_G
Cuts at: 826 1045 2747 3401 4234 5369 6050 6241 826
Size: 219 1702 654 833 1135 681 191 1471
Fragments arranged by size:
1702 1471 1135 833 681 654 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6886
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6886
SmaI CCC'GGG
Cuts at: 1872 2336 4213 1872
Size: 464 1877 4545
Fragments arranged by size:
4545 1877 464
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2660 2834 4990 5891 6153 820
Size: 197 926 717 174 2156 901 262 1553
Fragments arranged by size:
2156 1553 926 901 717 262 197 174
SnaBI TAC'GTA
Cuts at: 493 4677 493
Size: 4184 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6886
SphI G_CATG'C
Cuts at: 1132 4244 1132
Size: 3112 3774
SspI AAT'ATT
Cuts at: 5 52 4810 5
Size: 47 4758 2081
Fragments arranged by size:
4758 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6886
StyI C'CwwG_G
Cuts at: 513 1065 2182 2643 2725 3508 4079 4325 513
Size: 552 1117 461 82 783 571 246 3074
Fragments arranged by size:
3074 1117 783 571 552 461 246 82
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2132 3182 3560 4913 4930 5083 5268
Size: 1255 552 1050 378 1353 17 153 185
Cuts at: 5268 5713 325
Size: 445 1498
Fragments arranged by size:
1498 1353 1255 1050 552 445 378 185
153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3603 3858 5132
Size: 274 80 33 51 1443 1626 255 1274
Cuts at: 5132 6844 96
Size: 1712 138
Fragments arranged by size:
1712 1626 1443 1274 274 255 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6846 6878 6846
Size: 32 6854
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6886
XbaI T'CTAG_A
Cuts at: 4222 4222
Size: 6886
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2371 2431 2563 2633 4086 2371
Size: 60 132 70 1453 5171
Fragments arranged by size:
5171 1453 132 70 60
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6886
XmaI C'CCGG_G
Cuts at: 1870 2334 4211 1870
Size: 464 1877 4545
Fragments arranged by size:
4545 1877 464
XmnI GAAnn'nnTTC
Cuts at: 2351 5015 2351
Size: 2664 4222
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 2214 4694 276
Size: 53 83 186 936 680 2480 2468
Fragments arranged by size:
2480 2468 936 680 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI AvrII BamHI BanI BanII
BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI
Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI
BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI
ClaI CspCI DraI DraIII DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI
HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI
MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV
NheI NotI NspI PasI PciI PflMI PmeI PpiI
PshAI PsiI PspOMI PstI PvuI PvuII SacI SalI
SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AloI AscI BaeI BbeI BfrBI BsiWI BstZ17I EcoRV
FseI FspAI KasI NarI NruI NsiI PacI PfoI
PmlI PpuMI PspXI PsrI RsrII SacII SanDI SapI
SexAI SfiI SfoI SrfI SwaI