Restriction map for
pFN21AB5507
(Circular) (Six-base) MAPSORT of: pFN21AB5507.seq Check: 5271 from: 1 to: 6493
pFN21AB5507 6493 bp
With 182 enzymes: SgfI *
April 15, 2010 09:35 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4303 278
Size: 53 83 186 936 2767 2468
Fragments arranged by size:
2767 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3836 1937
Size: 1899 4594
Acc65I G'GTAC_C
Cuts at: 3814 3814
Size: 6493
AclI AA'CG_TT
Cuts at: 1563 4620 4993 1563
Size: 3057 373 3063
Fragments arranged by size:
3063 3057 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2276 2881 4556 5940 6208 1632
Size: 644 605 1675 1384 268 1917
Fragments arranged by size:
1917 1675 1384 644 605 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6296
AflIII A'CryG_T
Cuts at: 1203 3459 5392 6369 1203
Size: 2256 1933 977 1327
Fragments arranged by size:
2256 1933 1327 977
AgeI A'CCGG_T
Cuts at: 5324 5324
Size: 6493
AhdI GACnn_n'nnGTC
Cuts at: 5222 5222
Size: 6493
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6493
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 3179 1184
Size: 1995 4498
AlwNI CAG_nnn'CTG
Cuts at: 2405 3019 3547 4236 5808 2405
Size: 614 528 689 1572 3090
Fragments arranged by size:
3090 1572 689 614 528
ApaLI G'TGCA_C
Cuts at: 2463 4550 5706 2463
Size: 2087 1156 3250
Fragments arranged by size:
3250 2087 1156
ApoI r'AATT_y
Cuts at: 1493 2311 2976 3405 3802 4028 4180 6289 1493
Size: 818 665 429 397 226 152 2109 1697
Fragments arranged by size:
2109 1697 818 665 429 397 226 152
AseI AT'TA_AT
Cuts at: 160 1032 5047 160
Size: 872 4015 1606
Fragments arranged by size:
4015 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6493
AvaI C'yCGr_G
Cuts at: 1870 1943 2012 2553 2885 3818 1870
Size: 73 69 541 332 933 4545
Fragments arranged by size:
4545 933 541 332 73 69
BamHI G'GATC_C
Cuts at: 2851 3823 4190 2851
Size: 972 367 5154
Fragments arranged by size:
5154 972 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3814 5269 618
Size: 325 217 627 2027 1455 1842
Fragments arranged by size:
2027 1842 1455 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2013 2073 3812 1391
Size: 622 60 1739 4072
Fragments arranged by size:
4072 1739 622 60
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3327 928
Size: 971 1428 4094
Fragments arranged by size:
4094 1428 971
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3142 3176 4684 4718 1903
Size: 34 1205 34 1508 34 3678
Fragments arranged by size:
3678 1508 1205 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4385 5595 4385
Size: 1210 5283
BclI T'GATC_A
Cuts at: 1548 2836 1548
Size: 1288 5205
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 5104 136
Size: 107 122 71 631 605 3432 1525
Fragments arranged by size:
3432 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2911 6488 2911
Size: 3577 2916
BlpI GC'TnA_GC
Cuts at: 2072 3910 2072
Size: 1838 4655
Bme1580I G_kGCm'C
Cuts at: 1790 2467 4554 5710 1790
Size: 677 2087 1156 2573
Fragments arranged by size:
2573 2087 1156 677
BmgBI CAC'GTC
Cuts at: 1318 2301 3460 1318
Size: 983 1159 4351
Fragments arranged by size:
4351 1159 983
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2194 3293 3427
Size: 416 53 83 399 24 777 1099 134
Cuts at: 3427 4224 5182 442
Size: 797 958 1753
Fragments arranged by size:
1753 1099 958 797 777 416 399 134
83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6493
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2858 3041 3131 3411 5153 1239
Size: 1619 183 90 280 1742 2579
Fragments arranged by size:
2579 1742 1619 280 183 90
Bpu10I CC'TnA_GC
Cuts at: 2240 3534 2240
Size: 1294 5199
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4618 5483 5781 4618
Size: 865 298 5330
Fragments arranged by size:
5330 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 5156 882
Size: 617 3657 2219
Fragments arranged by size:
3657 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 4284 493
Size: 711 3080 2702
Fragments arranged by size:
3080 2702 711
BsaBI GATnn'nnATC
Cuts at: 6487 6487
Size: 6493
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4300 4682 5317 275
Size: 53 83 186 936 2767 382 635 1451
Fragments arranged by size:
2767 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4926 5324 5598 5745 6156 4926
Size: 398 274 147 411 5263
Fragments arranged by size:
5263 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 3178 3208 1178
Size: 30 165 30 1775 30 4463
Fragments arranged by size:
4463 1775 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 3378 3625 1186
Size: 2192 247 4054
Fragments arranged by size:
4054 2192 247
BseYI C'CCAG_C
Cuts at: 2398 5696 6192 2398
Size: 3298 496 2699
Fragments arranged by size:
3298 2699 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 3542 1163
Size: 573 1806 4114
Fragments arranged by size:
4114 1806 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2042 2103 3943 4704 5732 1540
Size: 275 186 41 61 1840 761 1028 2301
Fragments arranged by size:
2301 1840 1028 761 275 186 61 41
BsiHKAI G_wGCw'C
Cuts at: 2467 3003 4554 4639 5710 2467
Size: 536 1551 85 1071 3250
Fragments arranged by size:
3250 1551 1071 536 85
BsmI GAATG_Cn'
Cuts at: 2791 4004 4097 2791
Size: 1213 93 5187
Fragments arranged by size:
5187 1213 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2013 2073 2467 3003 3812 4554 4639
Size: 399 223 60 394 536 809 742 85
Cuts at: 4639 5710 1391
Size: 1071 2174
Fragments arranged by size:
2174 1071 809 742 536 399 394 223
85 60
BspEI T'CCGG_A
Cuts at: 6156 6156
Size: 6493
BspHI T'CATG_A
Cuts at: 4382 6112 4382
Size: 1730 4763
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2415 3333 3848 844
Size: 1571 918 515 3489
Fragments arranged by size:
3489 1571 918 515
BsrBI CCG'CTC
Cuts at: 1394 1823 3497 4380 1394
Size: 429 1674 883 3507
Fragments arranged by size:
3507 1674 883 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3501 4988 5162 65
Size: 1183 2253 1487 174 1396
Fragments arranged by size:
2253 1487 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 3285 5137 5324 1700
Size: 110 1475 1852 187 2869
Fragments arranged by size:
2869 1852 1475 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6493
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6493
BssSI C'ACGA_G
Cuts at: 2292 2762 4553 5565 2292
Size: 470 1791 1012 3220
Fragments arranged by size:
3220 1791 1012 470
BstAPI GCAn_nnn'nTGC
Cuts at: 794 3547 794
Size: 2753 3740
BstBI TT'CG_AA
Cuts at: 5297 5297
Size: 6493
BstEII G'GTnAC_C
Cuts at: 1184 2384 2772 1184
Size: 1200 388 4905
Fragments arranged by size:
4905 1200 388
BstYI r'GATC_y
Cuts at: 1972 2393 2578 2851 2911 3823 4190 4575 4592
Size: 421 185 273 60 912 367 385 17
Cuts at: 4592 6044 6130 6142 6488 1972
Size: 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 912 421 385 367 346 273
185 86 60 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2042 3172 513
Size: 552 939 38 1130 3834
Fragments arranged by size:
3834 1130 939 552 38
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2893 487
Size: 683 56 1667 4087
Fragments arranged by size:
4087 1667 683 56
BtsI GCAGTG_nn'
Cuts at: 2467 3482 4009 4803 4823 2467
Size: 1015 527 794 20 4137
Fragments arranged by size:
4137 1015 794 527 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6493
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6458
DraI TTT'AAA
Cuts at: 3797 3797
Size: 6493
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2352 5500 809
Size: 507 1036 3148 1802
Fragments arranged by size:
3148 1802 1036 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2100 3498 3940
Size: 54 1425 50 275 18 270 1398 442
Cuts at: 3940 4829 6458 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 1398 889 442 275 270 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2100 3940 1537
Size: 275 288 1840 4090
Fragments arranged by size:
4090 1840 288 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2634 2835 3558 3736 4423 1353
Size: 1281 201 723 178 687 3423
Fragments arranged by size:
3423 1281 723 687 201 178
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5080 5454 5600 387
Size: 1064 138 3491 374 146 1280
Fragments arranged by size:
3491 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2276 2858 2881 3041 3131 3411 4556
Size: 393 644 582 23 160 90 280 1145
Cuts at: 4556 5153 5940 6208 1239
Size: 597 787 268 1524
Fragments arranged by size:
1524 1145 787 644 597 582 393 280
268 160 90 23
EcoNI CCTnn'n_nnAGG
Cuts at: 3589 3589
Size: 6493
EcoO109I rG'GnC_Cy
Cuts at: 2093 3136 3544 2093
Size: 1043 408 5042
Fragments arranged by size:
5042 1043 408
EcoRI G'AATT_C
Cuts at: 3405 3802 4180 3405
Size: 397 378 5718
Fragments arranged by size:
5718 397 378
EcoRV GAT'ATC
Cuts at: 2932 3061 2932
Size: 129 6364
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2849 2996 2849
Size: 147 6346
FspI TGC'GCA
Cuts at: 4999 4999
Size: 6493
HaeII r_GCGC'y
Cuts at: 1678 5640 1678
Size: 3962 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2378 2410 2657 2689 5134
Size: 32 135 32 806 32 247 32 2445
Cuts at: 5134 5166 5208 5240 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 2445 806 247 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2430 2703 3837 4083 669
Size: 988 281 492 273 1134 246 3079
Fragments arranged by size:
3079 1134 988 492 281 273 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6493
HpaI GTT'AAC
Cuts at: 4083 4083
Size: 6493
KpnI G_GTAC'C
Cuts at: 3818 3818
Size: 6493
MfeI C'AATT_G
Cuts at: 4092 4092
Size: 6493
MluI A'CGCG_T
Cuts at: 6369 6369
Size: 6493
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2097 5607 5791 1305
Size: 792 3510 184 2007
Fragments arranged by size:
3510 2007 792 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4671
Fragments arranged by size:
4671 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2492 2762 3006 3686 4451 4810 4969
Size: 648 1326 270 244 680 765 359 159
Cuts at: 4969 518
Size: 2042
Fragments arranged by size:
2042 1326 765 680 648 359 270 244
159
MspA1I CmG'CkG
Cuts at: 1606 2044 2815 3909 4586 5734 5979 1606
Size: 438 771 1094 677 1148 245 2120
Fragments arranged by size:
2120 1148 1094 771 677 438 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 3172 513
Size: 552 939 1168 3834
Fragments arranged by size:
3834 1168 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6493
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6493
NotI GC'GGCC_GC
Cuts at: 3940 3940
Size: 6493
NspI r_CATG'y
Cuts at: 1132 1229 3685 3851 5396 1132
Size: 97 2456 166 1545 2229
Fragments arranged by size:
2456 2229 1545 166 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6493
PciI A'CATG_T
Cuts at: 5392 5392
Size: 6493
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6493
PmeI GTTT'AAAC
Cuts at: 3797 3797
Size: 6493
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3569 3601 4562 4594 6075 6107 3569
Size: 32 961 32 1481 32 3955
Fragments arranged by size:
3955 1481 961 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 3136 3136
Size: 6493
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6493
PsiI TTA'TAA
Cuts at: 4063 4063
Size: 6493
PstI C_TGCA'G
Cuts at: 830 3330 3543 3845 830
Size: 2500 213 302 3478
Fragments arranged by size:
3478 2500 302 213
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6493
PvuII CAG'CTG
Cuts at: 2815 2815
Size: 6493
SacII CC_GC'GG
Cuts at: 2045 2045
Size: 6493
SalI G'TCGA_C
Cuts at: 1936 3835 1936
Size: 1899 4594
SanDI GG'GwC_CC
Cuts at: 3136 3136
Size: 6493
SapI GCTCTTCn'nnn_
Cuts at: 2835 3736 2835
Size: 901 5592
SbfI CC_TGCA'GG
Cuts at: 3330 3845 3330
Size: 515 5978
ScaI AGT'ACT
Cuts at: 4741 6453 4741
Size: 1712 4781
SexAI A'CCwGG_T
Cuts at: 3276 3276
Size: 6493
SfcI C'TryA_G
Cuts at: 826 1045 2791 2803 3326 3539 3841 4976 5657
Size: 219 1746 12 523 213 302 1135 681
Cuts at: 5657 5848 826
Size: 191 1471
Fragments arranged by size:
1746 1471 1135 681 523 302 219 213
191 12
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6493
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6493
SmaI CCC'GGG
Cuts at: 1872 3820 1872
Size: 1948 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2885 4597 5498 5760 820
Size: 197 926 942 1712 901 262 1553
Fragments arranged by size:
1712 1553 942 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 4284 493
Size: 3791 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6493
SphI G_CATG'C
Cuts at: 1132 3685 3851 1132
Size: 2553 166 3774
Fragments arranged by size:
3774 2553 166
SspI AAT'ATT
Cuts at: 5 52 4417 5
Size: 47 4365 2081
Fragments arranged by size:
4365 2081 47
StuI AGG'CCT
Cuts at: 1517 2600 1517
Size: 1083 5410
StyI C'CwwG_G
Cuts at: 513 1065 2004 2304 3172 3932 513
Size: 552 939 300 868 760 3074
Fragments arranged by size:
3074 939 868 760 552 300
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 4520 4537 4690 4875 5320 325
Size: 1255 2940 17 153 185 445 1498
Fragments arranged by size:
2940 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4739 6451 96
Size: 274 80 33 51 1443 2762 1712 138
Fragments arranged by size:
2762 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3178 3210 6453 6485 3178
Size: 32 3243 32 3186
Fragments arranged by size:
3243 3186 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6493
XbaI T'CTAG_A
Cuts at: 3829 3829
Size: 6493
XhoI C'TCGA_G
Cuts at: 1943 2885 1943
Size: 942 5551
XmaI C'CCGG_G
Cuts at: 1870 3818 1870
Size: 1948 4545
XmnI GAAnn'nnTTC
Cuts at: 4622 4622
Size: 6493
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4301 276
Size: 53 83 186 936 2767 2468
Fragments arranged by size:
2767 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BcgI BciVI BclI
BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI
Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI
CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI
EcoNI EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I
HincII HindIII HpaI KpnI MfeI MluI MmeI MscI
MslI MspA1I NcoI NdeI NheI NotI NspI PasI
PciI PflMI PmeI PpiI PpuMI PshAI PsiI PstI
PvuI PvuII SacII SalI SanDI SapI SbfI ScaI
SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BbvCI BfrBI BplI BsiWI BsmBI BstXI BstZ17I Bsu36I
DraIII EcoICRI FseI FspAI KasI NaeI NarI NgoMIV
NruI NsiI PacI PfoI PmlI PspOMI PspXI PsrI
RsrII SacI SfiI SfoI SrfI SwaI XcmI