Restriction map for
pFN21AB5503
(Circular) (Six-base) MAPSORT of: pFN21AB5503.seq Check: 56 from: 1 to: 6370
pFN21AB5503 6370 bp
With 182 enzymes: SgfI *
April 15, 2010 09:31 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4180 278
Size: 53 83 186 936 2644 2468
Fragments arranged by size:
2644 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2733 3713 1937
Size: 796 980 4594
Fragments arranged by size:
4594 980 796
Acc65I G'GTAC_C
Cuts at: 3691 3691
Size: 6370
AclI AA'CG_TT
Cuts at: 1563 4497 4870 1563
Size: 2934 373 3063
Fragments arranged by size:
3063 2934 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2159 2680 4433 5817 6085 1632
Size: 527 521 1753 1384 268 1917
Fragments arranged by size:
1917 1753 1384 527 521 268
AfeI AGC'GCT
Cuts at: 2647 2647
Size: 6370
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6173
AflIII A'CryG_T
Cuts at: 1203 5269 6246 1203
Size: 4066 977 1327
Fragments arranged by size:
4066 1327 977
AgeI A'CCGG_T
Cuts at: 5201 5201
Size: 6370
AhdI GACnn_n'nnGTC
Cuts at: 2719 5099 2719
Size: 2380 3990
AleI CACnn'nnGTG
Cuts at: 1166 2204 2261 1166
Size: 1038 57 5275
Fragments arranged by size:
5275 1038 57
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6370
AlwNI CAG_nnn'CTG
Cuts at: 3349 4113 5685 3349
Size: 764 1572 4034
Fragments arranged by size:
4034 1572 764
ApaI G_GGCC'C
Cuts at: 2496 2496
Size: 6370
ApaLI G'TGCA_C
Cuts at: 4427 5583 4427
Size: 1156 5214
ApoI r'AATT_y
Cuts at: 1493 3679 3905 4057 6166 1493
Size: 2186 226 152 2109 1697
Fragments arranged by size:
2186 2109 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4924 160
Size: 872 3892 1606
Fragments arranged by size:
3892 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6370
AvaI C'yCGr_G
Cuts at: 1870 1943 2046 3695 1870
Size: 73 103 1649 4545
Fragments arranged by size:
4545 1649 103 73
BamHI G'GATC_C
Cuts at: 2939 3700 4067 2939
Size: 761 367 5242
Fragments arranged by size:
5242 761 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2191 2792 3450 3691 5146
Size: 325 217 627 404 601 658 241 1455
Cuts at: 5146 618
Size: 1842
Fragments arranged by size:
1842 1455 658 627 601 404 325 241
217
BanII G_rGCy'C
Cuts at: 1391 2426 2496 3292 3689 1391
Size: 1035 70 796 397 4072
Fragments arranged by size:
4072 1035 796 397 70
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2120 3668 928
Size: 971 221 1548 3630
Fragments arranged by size:
3630 1548 971 221
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4561 4595 1903
Size: 34 2624 34 3678
Fragments arranged by size:
3678 2624 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4262 5472 4262
Size: 1210 5160
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6370
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2009 2018 4981
Size: 107 122 71 631 605 337 9 2963
Cuts at: 4981 136
Size: 1525
Fragments arranged by size:
2963 1525 631 605 337 122 107 71
9
BglII A'GATC_T
Cuts at: 3621 6365 3621
Size: 2744 3626
BlpI GC'TnA_GC
Cuts at: 3204 3787 3204
Size: 583 5787
Bme1580I G_kGCm'C
Cuts at: 1790 2229 2496 3453 3600 4431 5587 1790
Size: 439 267 957 147 831 1156 2573
Fragments arranged by size:
2573 1156 957 831 439 267 147
BmgBI CAC'GTC
Cuts at: 1318 3385 1318
Size: 2067 4303
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2746 2915 4101
Size: 416 53 83 399 24 1329 169 1186
Cuts at: 4101 5059 442
Size: 958 1753
Fragments arranged by size:
1753 1329 1186 958 416 399 169 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6370
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2679 2679
Size: 6370
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2860 3021 3650 3667 5030 1239
Size: 1621 161 629 17 1363 2579
Fragments arranged by size:
2579 1621 1363 629 161 17
Bpu10I CC'TnA_GC
Cuts at: 2991 2991
Size: 6370
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2651 4495 5360 5658 2651
Size: 1844 865 298 3363
Fragments arranged by size:
3363 1844 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2117 3626 5033 882
Size: 617 618 1509 1407 2219
Fragments arranged by size:
2219 1509 1407 618 617
BsaAI yAC'GTr
Cuts at: 493 1204 2440 2986 4161 493
Size: 711 1236 546 1175 2702
Fragments arranged by size:
2702 1236 1175 711 546
BsaBI GATnn'nnATC
Cuts at: 6364 6364
Size: 6370
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3443 4177 4559 5194
Size: 53 83 186 936 1910 734 382 635
Cuts at: 5194 275
Size: 1451
Fragments arranged by size:
1910 1451 936 734 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 4803 5201 5475 5622 6033 4803
Size: 398 274 147 411 5140
Fragments arranged by size:
5140 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2345 2375 1178
Size: 30 165 30 942 30 5173
Fragments arranged by size:
5173 942 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2363 2366 2446 3515 1186
Size: 1177 3 80 1069 4041
Fragments arranged by size:
4041 1177 1069 80 3
BseYI C'CCAG_C
Cuts at: 2092 2935 3069 3210 5573 6069 2092
Size: 843 134 141 2363 496 2393
Fragments arranged by size:
2393 2363 843 496 141 134
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5797
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2013 3820 4581 5609 1540
Size: 275 186 12 1807 761 1028 2301
Fragments arranged by size:
2301 1807 1028 761 275 186 12
BsiHKAI G_wGCw'C
Cuts at: 2970 4431 4516 5587 2970
Size: 1461 85 1071 3753
Fragments arranged by size:
3753 1461 1071 85
BsmI GAATG_Cn'
Cuts at: 2173 2788 3881 3974 2173
Size: 615 1093 93 4569
Fragments arranged by size:
4569 1093 615 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2229 2426 2496 2970 3292 3453 3600
Size: 399 439 197 70 474 322 161 147
Cuts at: 3600 3689 4431 4516 5587 1391
Size: 89 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 474 439 399 322 197
161 147 89 85 70
BspEI T'CCGG_A
Cuts at: 6033 6033
Size: 6370
BspHI T'CATG_A
Cuts at: 4259 5989 4259
Size: 1730 4640
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3725 844
Size: 2881 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 2104 4257 1394
Size: 429 281 2153 3507
Fragments arranged by size:
3507 2153 429 281
BsrDI GCAATG_nn'
Cuts at: 65 1248 2844 4865 5039 65
Size: 1183 1596 2021 174 1396
Fragments arranged by size:
2021 1596 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 3276 5014 5201 1700
Size: 110 1466 1738 187 2869
Fragments arranged by size:
2869 1738 1466 187 110
BsrGI T'GTAC_A
Cuts at: 96 3012 3399 96
Size: 2916 387 3067
Fragments arranged by size:
3067 2916 387
BssHII G'CGCG_C
Cuts at: 1925 2056 1925
Size: 131 6239
BssSI C'ACGA_G
Cuts at: 4430 5442 4430
Size: 1012 5358
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6370
BstBI TT'CG_AA
Cuts at: 5174 5174
Size: 6370
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6370
BstXI CCAn_nnnn'nTGG
Cuts at: 2092 2159 2092
Size: 67 6303
BstYI r'GATC_y
Cuts at: 1972 2939 3621 3700 4067 4452 4469 5921 6007
Size: 967 682 79 367 385 17 1452 86
Cuts at: 6007 6019 6365 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 967 682 385 367 346 86
79 17 12
Bsu36I CC'TnA_GG
Cuts at: 2406 2406
Size: 6370
BtgI C'CryG_G
Cuts at: 513 1065 2004 2118 513
Size: 552 939 114 4765
Fragments arranged by size:
4765 939 552 114
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5631
Fragments arranged by size:
5631 683 56
BtsI GCAGTG_nn'
Cuts at: 2377 2514 3886 4680 4700 2377
Size: 137 1372 794 20 4047
Fragments arranged by size:
4047 1372 794 137 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6370
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6335
DraI TTT'AAA
Cuts at: 3674 3674
Size: 6370
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 3640 5377 809
Size: 507 2324 1737 1802
Fragments arranged by size:
2324 1802 1737 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2010 2082 2551
Size: 54 1425 50 275 18 180 72 469
Cuts at: 2551 3042 3309 3817 4706 6335 8
Size: 491 267 508 889 1629 43
Fragments arranged by size:
1629 1425 889 508 491 469 275 267
180 72 54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2010 3817 1537
Size: 275 198 1807 4090
Fragments arranged by size:
4090 1807 275 198
EarI CTCTTCn'nnn_
Cuts at: 1353 2072 2648 3429 3584 4300 1353
Size: 719 576 781 155 716 3423
Fragments arranged by size:
3423 781 719 716 576 155
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2171 4957 5331 5477 387
Size: 1064 138 582 2786 374 146 1280
Fragments arranged by size:
2786 1280 1064 582 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2159 2680 2860 3021 3650 3667 4433
Size: 393 527 521 180 161 629 17 766
Cuts at: 4433 5030 5817 6085 1239
Size: 597 787 268 1524
Fragments arranged by size:
1524 787 766 629 597 527 521 393
268 180 161 17
EcoNI CCTnn'n_nnAGG
Cuts at: 2329 2630 2329
Size: 301 6069
EcoO109I rG'GnC_Cy
Cuts at: 2356 3239 2356
Size: 883 5487
EcoRI G'AATT_C
Cuts at: 3679 4057 3679
Size: 378 5992
EcoRV GAT'ATC
Cuts at: 2445 2445
Size: 6370
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2269 2692 2269
Size: 423 5947
FspI TGC'GCA
Cuts at: 4876 4876
Size: 6370
HaeII r_GCGC'y
Cuts at: 1678 2649 2764 3036 5517 1678
Size: 971 115 272 2481 2531
Fragments arranged by size:
2531 2481 971 272 115
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2345 2377 2705 2737 3374
Size: 32 135 32 773 32 328 32 637
Cuts at: 3374 3406 3428 3460 5011 5043 5085 5117 1373
Size: 32 22 32 1551 32 42 32 2626
Fragments arranged by size:
2626 1551 773 637 328 135 42 32
32 32 32 32 32 32 32 22
HincII GTy'rAC
Cuts at: 669 1657 1938 3097 3714 3960 669
Size: 988 281 1159 617 246 3079
Fragments arranged by size:
3079 1159 988 617 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6370
HpaI GTT'AAC
Cuts at: 3960 3960
Size: 6370
KpnI G_GTAC'C
Cuts at: 3695 3695
Size: 6370
MfeI C'AATT_G
Cuts at: 3969 3969
Size: 6370
MluI A'CGCG_T
Cuts at: 6246 6246
Size: 6370
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5484 5668 1305
Size: 4179 184 2007
Fragments arranged by size:
4179 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2084 3044 3311 10
Size: 54 1425 343 252 960 267 3069
Fragments arranged by size:
3069 1425 960 343 267 252 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2204 2261 4328 4687 4846 518
Size: 648 1038 57 2067 359 159 2042
Fragments arranged by size:
2067 2042 1038 648 359 159 57
MspA1I CmG'CkG
Cuts at: 1606 2798 3010 3069 3214 3786 4463 5611 5856
Size: 1192 212 59 145 572 677 1148 245
Cuts at: 5856 1606
Size: 2120
Fragments arranged by size:
2120 1192 1148 677 572 245 212 145
59
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4879
Fragments arranged by size:
4879 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6370
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6370
NotI GC'GGCC_GC
Cuts at: 2010 3817 2010
Size: 1807 4563
NspI r_CATG'y
Cuts at: 1132 1229 2896 3728 5273 1132
Size: 97 1667 832 1545 2229
Fragments arranged by size:
2229 1667 1545 832 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6370
PciI A'CATG_T
Cuts at: 5269 5269
Size: 6370
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6370
PfoI T'CCnGG_A
Cuts at: 3627 3644 3627
Size: 17 6353
PmeI GTTT'AAAC
Cuts at: 3674 3674
Size: 6370
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4439 4471 5952 5984 4439
Size: 32 1481 32 4825
Fragments arranged by size:
4825 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6370
PsiI TTA'TAA
Cuts at: 3940 3940
Size: 6370
PspOMI G'GGCC_C
Cuts at: 2492 2492
Size: 6370
PstI C_TGCA'G
Cuts at: 830 3722 830
Size: 2892 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6370
PvuII CAG'CTG
Cuts at: 3214 3214
Size: 6370
SalI G'TCGA_C
Cuts at: 1936 3712 1936
Size: 1776 4594
SbfI CC_TGCA'GG
Cuts at: 3722 3722
Size: 6370
ScaI AGT'ACT
Cuts at: 4618 6330 4618
Size: 1712 4658
SfcI C'TryA_G
Cuts at: 826 1045 3408 3718 4853 5534 5725 826
Size: 219 2363 310 1135 681 191 1471
Fragments arranged by size:
2363 1471 1135 681 310 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6370
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6370
SmaI CCC'GGG
Cuts at: 1872 2048 3697 1872
Size: 176 1649 4545
Fragments arranged by size:
4545 1649 176
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2666 4474 5375 5637 820
Size: 197 926 723 1808 901 262 1553
Fragments arranged by size:
1808 1553 926 901 723 262 197
SnaBI TAC'GTA
Cuts at: 493 4161 493
Size: 3668 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6370
SphI G_CATG'C
Cuts at: 1132 3728 1132
Size: 2596 3774
SspI AAT'ATT
Cuts at: 5 52 4294 5
Size: 47 4242 2081
Fragments arranged by size:
4242 2081 47
StuI AGG'CCT
Cuts at: 1517 2324 2537 1517
Size: 807 213 5350
Fragments arranged by size:
5350 807 213
StyI C'CwwG_G
Cuts at: 513 1065 2004 3809 513
Size: 552 939 1805 3074
Fragments arranged by size:
3074 1805 939 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3282 4397 4414 4567 4752 5197 325
Size: 1255 1702 1115 17 153 185 445 1498
Fragments arranged by size:
1702 1498 1255 1115 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3012 3399 4616
Size: 274 80 33 51 1443 1035 387 1217
Cuts at: 4616 6328 96
Size: 1712 138
Fragments arranged by size:
1712 1443 1217 1035 387 274 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6330 6362 6330
Size: 32 6338
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6370
XbaI T'CTAG_A
Cuts at: 3706 3706
Size: 6370
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2637 2884 2637
Size: 247 6123
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6370
XmaI C'CCGG_G
Cuts at: 1870 2046 3695 1870
Size: 176 1649 4545
Fragments arranged by size:
4545 1649 176
XmnI GAAnn'nnTTC
Cuts at: 4499 4499
Size: 6370
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4178 276
Size: 53 83 186 936 2644 2468
Fragments arranged by size:
2644 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI
Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI
EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI
EcoRV FalI FspI HaeII Hin4I HincII HindIII HpaI
KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI
NdeI NheI NotI NspI PasI PciI PflMI PfoI
PmeI PpiI PshAI PsiI PspOMI PstI PvuI PvuII
SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AloI AscI AvrII BaeI BbeI BbvCI BfrBI
BsiWI BsmBI BstZ17I DraIII EcoICRI FseI FspAI KasI
NaeI NarI NgoMIV NruI NsiI PacI PmlI PpuMI
PspXI PsrI RsrII SacI SacII SanDI SapI SexAI
SfiI SfoI SrfI SwaI