Restriction map for
pFN21AB5502
(Circular) (Six-base) MAPSORT of: pFN21AB5502.seq Check: 4146 from: 1 to: 6973
pFN21AB5502 6973 bp
With 182 enzymes: SgfI *
July 22, 2016 16:51 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4783 278
Size: 53 83 186 936 3247 2468
Fragments arranged by size:
3247 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 4088 4316 1937
Size: 2151 228 4594
Fragments arranged by size:
4594 2151 228
Acc65I G'GTAC_C
Cuts at: 4294 4294
Size: 6973
AclI AA'CG_TT
Cuts at: 1563 5100 5473 1563
Size: 3537 373 3063
Fragments arranged by size:
3537 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2192 3339 3354 3869 5036 6420 6688 1632
Size: 560 1147 15 515 1167 1384 268 1917
Fragments arranged by size:
1917 1384 1167 1147 560 515 268 15
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6776
AflIII A'CryG_T
Cuts at: 1203 5872 6849 1203
Size: 4669 977 1327
Fragments arranged by size:
4669 1327 977
AgeI A'CCGG_T
Cuts at: 5804 5804
Size: 6973
AhdI GACnn_n'nnGTC
Cuts at: 5702 5702
Size: 6973
AleI CACnn'nnGTG
Cuts at: 1166 3992 1166
Size: 2826 4147
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6973
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3546 3578 3546
Size: 32 6941
AlwNI CAG_nnn'CTG
Cuts at: 2609 3202 3314 4716 6288 2609
Size: 593 112 1402 1572 3294
Fragments arranged by size:
3294 1572 1402 593 112
ApaLI G'TGCA_C
Cuts at: 2924 5030 6186 2924
Size: 2106 1156 3711
Fragments arranged by size:
3711 2106 1156
ApoI r'AATT_y
Cuts at: 1493 2529 4282 4508 4660 6769 1493
Size: 1036 1753 226 152 2109 1697
Fragments arranged by size:
2109 1753 1697 1036 226 152
AseI AT'TA_AT
Cuts at: 160 1032 3543 5527 160
Size: 872 2511 1984 1606
Fragments arranged by size:
2511 1984 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6973
AvaI C'yCGr_G
Cuts at: 1870 1943 3168 4298 1870
Size: 73 1225 1130 4545
Fragments arranged by size:
4545 1225 1130 73
BamHI G'GATC_C
Cuts at: 4303 4670 4303
Size: 367 6606
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2198 4294 5749 618
Size: 325 217 627 411 2096 1455 1842
Fragments arranged by size:
2096 1842 1455 627 411 325 217
BanII G_rGCy'C
Cuts at: 1391 3014 4292 1391
Size: 1623 1278 4072
Fragments arranged by size:
4072 1623 1278
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3102 928
Size: 971 1203 4799
Fragments arranged by size:
4799 1203 971
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 5164 5198 1903
Size: 34 3227 34 3678
Fragments arranged by size:
3678 3227 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3346 4865 6075 3346
Size: 1519 1210 4244
Fragments arranged by size:
4244 1519 1210
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6973
BfrBI ATG'CAT
Cuts at: 3188 3188
Size: 6973
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 5584 136
Size: 107 122 71 631 605 3912 1525
Fragments arranged by size:
3912 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2218 6968 2218
Size: 4750 2223
BlpI GC'TnA_GC
Cuts at: 4390 4390
Size: 6973
Bme1580I G_kGCm'C
Cuts at: 1790 2928 3948 5034 6190 1790
Size: 1138 1020 1086 1156 2573
Fragments arranged by size:
2573 1156 1138 1086 1020
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6973
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 4704 5662 442
Size: 416 53 83 399 24 3287 958 1753
Fragments arranged by size:
3287 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6973
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2993 3007 3515 5633 1239
Size: 1754 14 508 2118 2579
Fragments arranged by size:
2579 2118 1754 508 14
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2839 5098 5963 6261 2839
Size: 2259 865 298 3551
Fragments arranged by size:
3551 2259 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 5636 882
Size: 617 4137 2219
Fragments arranged by size:
4137 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 4764 493
Size: 711 3560 2702
Fragments arranged by size:
3560 2702 711
BsaBI GATnn'nnATC
Cuts at: 2183 6967 2183
Size: 4784 2189
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4780 5162 5797 275
Size: 53 83 186 936 3247 382 635 1451
Fragments arranged by size:
3247 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4062 5406 5804 6078 6225 6636 4062
Size: 1344 398 274 147 411 4399
Fragments arranged by size:
4399 1344 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2589 2619 1178
Size: 30 165 30 1186 30 5532
Fragments arranged by size:
5532 1186 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2503 2975 3305 4097 1186
Size: 1317 472 330 792 4062
Fragments arranged by size:
4062 1317 792 472 330
BseYI C'CCAG_C
Cuts at: 3307 6176 6672 3307
Size: 2869 496 3608
Fragments arranged by size:
3608 2869 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2939 3002 1163
Size: 573 1203 63 5134
Fragments arranged by size:
5134 1203 573 63
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4423 5184 6212 1540
Size: 275 186 2422 761 1028 2301
Fragments arranged by size:
2422 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2928 5034 5119 6190 2928
Size: 2106 85 1071 3711
Fragments arranged by size:
3711 2106 1071 85
BsmI GAATG_Cn'
Cuts at: 4046 4484 4577 4046
Size: 438 93 6442
Fragments arranged by size:
6442 438 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2928 3014 3948 4292 5034 5119 6190
Size: 399 1138 86 934 344 742 85 1071
Cuts at: 6190 1391
Size: 2174
Fragments arranged by size:
2174 1138 1071 934 742 399 344 86
85
BspEI T'CCGG_A
Cuts at: 4062 6636 4062
Size: 2574 4399
BspHI T'CATG_A
Cuts at: 4862 6592 4862
Size: 1730 5243
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2538 4248 4328 844
Size: 1694 1710 80 3489
Fragments arranged by size:
3489 1710 1694 80
BsrBI CCG'CTC
Cuts at: 1394 1823 2977 3748 4860 1394
Size: 429 1154 771 1112 3507
Fragments arranged by size:
3507 1154 1112 771 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 5468 5642 65
Size: 1183 4220 174 1396
Fragments arranged by size:
4220 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 5617 5804 1700
Size: 110 3807 187 2869
Fragments arranged by size:
3807 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6973
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6973
BssSI C'ACGA_G
Cuts at: 2485 2957 5033 6045 2485
Size: 472 2076 1012 3413
Fragments arranged by size:
3413 2076 1012 472
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6973
BstBI TT'CG_AA
Cuts at: 5777 5777
Size: 6973
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6973
BstXI CCAn_nnnn'nTGG
Cuts at: 2195 4137 2195
Size: 1942 5031
BstYI r'GATC_y
Cuts at: 1972 2184 2218 4303 4670 5055 5072 6524 6610
Size: 212 34 2085 367 385 17 1452 86
Cuts at: 6610 6622 6968 1972
Size: 12 346 1977
Fragments arranged by size:
2085 1977 1452 385 367 346 212 86
34 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2640 513
Size: 552 1575 4846
Fragments arranged by size:
4846 1575 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2044 487
Size: 683 56 818 5416
Fragments arranged by size:
5416 818 683 56
BtsI GCAGTG_nn'
Cuts at: 4489 5283 5303 4489
Size: 794 20 6159
Fragments arranged by size:
6159 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6973
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6938
DraI TTT'AAA
Cuts at: 2502 4277 2502
Size: 1775 5198
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5980 809
Size: 507 4664 1802
Fragments arranged by size:
4664 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2084 2316 4420
Size: 54 1425 50 275 18 254 232 2104
Cuts at: 4420 5309 6938 8
Size: 889 1629 43
Fragments arranged by size:
2104 1629 1425 889 275 254 232 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 4420 1537
Size: 275 2608 4090
Fragments arranged by size:
4090 2608 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2789 3380 4903 1353
Size: 1436 591 1523 3423
Fragments arranged by size:
3423 1523 1436 591
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5560 5934 6080 387
Size: 1064 138 3971 374 146 1280
Fragments arranged by size:
3971 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2192 2993 3007 3339 3354 3515 3869
Size: 393 560 801 14 332 15 161 354
Cuts at: 3869 5036 5633 6420 6688 1239
Size: 1167 597 787 268 1524
Fragments arranged by size:
1524 1167 801 787 597 560 393 354
332 268 161 15 14
EcoNI CCTnn'n_nnAGG
Cuts at: 2957 2957
Size: 6973
EcoO109I rG'GnC_Cy
Cuts at: 2445 2464 3392 2445
Size: 19 928 6026
Fragments arranged by size:
6026 928 19
EcoRI G'AATT_C
Cuts at: 4282 4660 4282
Size: 378 6595
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2791 3839 2791
Size: 1048 5925
FspI TGC'GCA
Cuts at: 5479 5479
Size: 6973
HaeII r_GCGC'y
Cuts at: 1678 6120 1678
Size: 4442 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2117 2149 3866 3898 3951
Size: 32 135 32 545 32 1717 32 53
Cuts at: 3951 3983 5614 5646 5688 5720 1373
Size: 32 1631 32 42 32 2626
Fragments arranged by size:
2626 1717 1631 545 135 53 42 32
32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 4089 4317 4563 669
Size: 988 281 2151 228 246 3079
Fragments arranged by size:
3079 2151 988 281 246 228
HindIII A'AGCT_T
Cuts at: 748 2496 3179 3665 748
Size: 1748 683 486 4056
Fragments arranged by size:
4056 1748 683 486
HpaI GTT'AAC
Cuts at: 4563 4563
Size: 6973
KpnI G_GTAC'C
Cuts at: 4298 4298
Size: 6973
MfeI C'AATT_G
Cuts at: 4572 4572
Size: 6973
MluI A'CGCG_T
Cuts at: 6849 6849
Size: 6973
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 6087 6271 1305
Size: 4782 184 2007
Fragments arranged by size:
4782 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2318 10
Size: 54 1425 343 486 4665
Fragments arranged by size:
4665 1425 486 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3992 4931 5290 5449 518
Size: 648 2826 939 359 159 2042
Fragments arranged by size:
2826 2042 939 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2083 2363 4389 5066 6214 6459 1606
Size: 477 280 2026 677 1148 245 2120
Fragments arranged by size:
2120 2026 1148 677 477 280 245
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 6421
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6973
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6973
NotI GC'GGCC_GC
Cuts at: 4420 4420
Size: 6973
NsiI A_TGCA'T
Cuts at: 3190 3190
Size: 6973
NspI r_CATG'y
Cuts at: 1132 1229 4331 5876 1132
Size: 97 3102 1545 2229
Fragments arranged by size:
3102 2229 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6973
PciI A'CATG_T
Cuts at: 5872 5872
Size: 6973
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2432 2800 2911 3151 3788 1108
Size: 1324 368 111 240 637 4293
Fragments arranged by size:
4293 1324 637 368 240 111
PfoI T'CCnGG_A
Cuts at: 2970 3023 3531 3567 4136 2970
Size: 53 508 36 569 5807
Fragments arranged by size:
5807 569 508 53 36
PmeI GTTT'AAAC
Cuts at: 4277 4277
Size: 6973
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5042 5074 6555 6587 5042
Size: 32 1481 32 5428
Fragments arranged by size:
5428 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2445 2464 3392 2445
Size: 19 928 6026
Fragments arranged by size:
6026 928 19
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6973
PsiI TTA'TAA
Cuts at: 4543 4543
Size: 6973
PstI C_TGCA'G
Cuts at: 830 2262 3198 3481 4230 4325 830
Size: 1432 936 283 749 95 3478
Fragments arranged by size:
3478 1432 936 749 283 95
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6973
PvuII CAG'CTG
Cuts at: 2363 2363
Size: 6973
SalI G'TCGA_C
Cuts at: 1936 4087 4315 1936
Size: 2151 228 4594
Fragments arranged by size:
4594 2151 228
SanDI GG'GwC_CC
Cuts at: 2445 2445
Size: 6973
SbfI CC_TGCA'GG
Cuts at: 4325 4325
Size: 6973
ScaI AGT'ACT
Cuts at: 5221 6933 5221
Size: 1712 5261
SfcI C'TryA_G
Cuts at: 826 1045 2258 3194 3477 4226 4321 5456 6137
Size: 219 1213 936 283 749 95 1135 681
Cuts at: 6137 6328 826
Size: 191 1471
Fragments arranged by size:
1471 1213 1135 936 749 681 283 219
191 95
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6973
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6973
SmaI CCC'GGG
Cuts at: 1872 3170 4300 1872
Size: 1298 1130 4545
Fragments arranged by size:
4545 1298 1130
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2818 5077 5978 6240 820
Size: 197 926 875 2259 901 262 1553
Fragments arranged by size:
2259 1553 926 901 875 262 197
SnaBI TAC'GTA
Cuts at: 493 4764 493
Size: 4271 2702
SpeI A'CTAG_T
Cuts at: 152 2249 3515 152
Size: 2097 1266 3610
Fragments arranged by size:
3610 2097 1266
SphI G_CATG'C
Cuts at: 1132 4331 1132
Size: 3199 3774
SspI AAT'ATT
Cuts at: 5 52 2650 4897 5
Size: 47 2598 2247 2081
Fragments arranged by size:
2598 2247 2081 47
StuI AGG'CCT
Cuts at: 1517 2169 1517
Size: 652 6321
StyI C'CwwG_G
Cuts at: 513 1065 3621 4412 513
Size: 552 2556 791 3074
Fragments arranged by size:
3074 2556 791 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2886 3053 5000 5017 5170 5355 5800
Size: 1255 1306 167 1947 17 153 185 445
Cuts at: 5800 325
Size: 1498
Fragments arranged by size:
1947 1498 1306 1255 445 185 167 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2557 3030 4071
Size: 274 80 33 51 1443 580 473 1041
Cuts at: 4071 5219 6931 96
Size: 1148 1712 138
Fragments arranged by size:
1712 1443 1148 1041 580 473 274 138
80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2859 2891 6933 6965 2859
Size: 32 4042 32 2867
Fragments arranged by size:
4042 2867 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 3106 1316
Size: 1790 5183
XbaI T'CTAG_A
Cuts at: 4309 4309
Size: 6973
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2011 3789 2011
Size: 1778 5195
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6973
XmaI C'CCGG_G
Cuts at: 1870 3168 4298 1870
Size: 1298 1130 4545
Fragments arranged by size:
4545 1298 1130
XmnI GAAnn'nnTTC
Cuts at: 5102 5102
Size: 6973
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4781 276
Size: 53 83 186 936 3247 2468
Fragments arranged by size:
3247 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI
BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI
BtsI ClaI CspCI DraI DrdI EaeI EagI EarI
EciI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII
Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI
MscI MslI MspA1I NcoI NdeI NheI NotI NsiI
NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI
PshAI PsiI PstI PvuI PvuII SalI SanDI SbfI
ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI ApaI AscI AvrII BaeI BbeI BbvCI
BplI Bpu10I BsiWI BsmBI BstZ17I Bsu36I DraIII EcoICRI
EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI
PacI PmlI PspOMI PspXI PsrI RsrII SacI SacII
SapI SexAI SfiI SfoI SrfI SwaI