Restriction map for
pFN21AB5497
(Circular) (Six-base) MAPSORT of: pFN21AB5497.seq Check: 6275 from: 1 to: 6142
pFN21AB5497 6142 bp
With 182 enzymes: SgfI *
October 20, 2009 10:35 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3952 278
Size: 53 83 186 936 2416 2468
Fragments arranged by size:
2468 2416 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3485 1937
Size: 1548 4594
Acc65I G'GTAC_C
Cuts at: 3463 3463
Size: 6142
AclI AA'CG_TT
Cuts at: 1563 4269 4642 1563
Size: 2706 373 3063
Fragments arranged by size:
3063 2706 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2072 3367 4205 5589 5857 1632
Size: 440 1295 838 1384 268 1917
Fragments arranged by size:
1917 1384 1295 838 440 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5945
AflIII A'CryG_T
Cuts at: 1203 5041 6018 1203
Size: 3838 977 1327
Fragments arranged by size:
3838 1327 977
AgeI A'CCGG_T
Cuts at: 4973 4973
Size: 6142
AhdI GACnn_n'nnGTC
Cuts at: 4871 4871
Size: 6142
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6142
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6142
AlwNI CAG_nnn'CTG
Cuts at: 2275 2302 3885 5457 2275
Size: 27 1583 1572 2960
Fragments arranged by size:
2960 1583 1572 27
ApaLI G'TGCA_C
Cuts at: 4199 5355 4199
Size: 1156 4986
ApoI r'AATT_y
Cuts at: 1493 2997 3043 3081 3249 3451 3677 3829 5938
Size: 1504 46 38 168 202 226 152 2109
Cuts at: 5938 1493
Size: 1697
Fragments arranged by size:
2109 1697 1504 226 202 168 152 46
38
AseI AT'TA_AT
Cuts at: 160 1032 4696 160
Size: 872 3664 1606
Fragments arranged by size:
3664 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6142
AvaI C'yCGr_G
Cuts at: 1870 1943 2199 2249 2331 3467 1870
Size: 73 256 50 82 1136 4545
Fragments arranged by size:
4545 1136 256 82 73 50
BamHI G'GATC_C
Cuts at: 3472 3839 3472
Size: 367 5775
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3463 4918 618
Size: 325 217 627 1676 1455 1842
Fragments arranged by size:
1842 1676 1455 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2229 2325 2654 3102 3461 1391
Size: 838 96 329 448 359 4072
Fragments arranged by size:
4072 838 448 359 329 96
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 5171
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4333 4367 1903
Size: 34 2396 34 3678
Fragments arranged by size:
3678 2396 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3038 4034 5244 3038
Size: 996 1210 3936
Fragments arranged by size:
3936 1210 996
BclI T'GATC_A
Cuts at: 1548 2304 1548
Size: 756 5386
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4753 136
Size: 107 122 71 631 605 3081 1525
Fragments arranged by size:
3081 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6137 6137
Size: 6142
BlpI GC'TnA_GC
Cuts at: 3321 3559 3321
Size: 238 5904
Bme1580I G_kGCm'C
Cuts at: 1790 2029 4203 5359 1790
Size: 239 2174 1156 2573
Fragments arranged by size:
2573 2174 1156 239
BmgBI CAC'GTC
Cuts at: 1318 2368 1318
Size: 1050 5092
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3873 4831 442
Size: 416 53 83 399 24 2456 958 1753
Fragments arranged by size:
2456 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6142
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2297 2309 2312 2402 3027 3279 4802 1239
Size: 1058 12 3 90 625 252 1523 2579
Fragments arranged by size:
2579 1523 1058 625 252 90 12 3
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4267 5132 5430 4267
Size: 865 298 4979
Fragments arranged by size:
4979 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2180 2246 4805 882
Size: 617 681 66 2559 2219
Fragments arranged by size:
2559 2219 681 617 66
BsaAI yAC'GTr
Cuts at: 493 1204 3933 493
Size: 711 2729 2702
Fragments arranged by size:
2729 2702 711
BsaBI GATnn'nnATC
Cuts at: 6136 6136
Size: 6142
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3949 4331 4966 275
Size: 53 83 186 936 2416 382 635 1451
Fragments arranged by size:
2416 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4575 4973 5247 5394 5805 4575
Size: 398 274 147 411 4912
Fragments arranged by size:
4912 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2138 2168 1178
Size: 30 165 30 735 30 5152
Fragments arranged by size:
5152 735 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2477 2561 2899 3110 3137 1186
Size: 1291 84 338 211 27 4191
Fragments arranged by size:
4191 1291 338 211 84 27
BseYI C'CCAG_C
Cuts at: 5345 5841 5345
Size: 496 5646
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2357 1163
Size: 573 621 4948
Fragments arranged by size:
4948 621 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3592 4353 5381 1540
Size: 275 186 1591 761 1028 2301
Fragments arranged by size:
2301 1591 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2229 2325 4203 4288 5359 2229
Size: 96 1878 85 1071 3012
Fragments arranged by size:
3012 1878 1071 96 85
BsmI GAATG_Cn'
Cuts at: 2977 3653 3746 2977
Size: 676 93 5373
Fragments arranged by size:
5373 676 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2029 2229 2325 2654 3102 3461 4203
Size: 399 239 200 96 329 448 359 742
Cuts at: 4203 4288 5359 1391
Size: 85 1071 2174
Fragments arranged by size:
2174 1071 742 448 399 359 329 239
200 96 85
BspEI T'CCGG_A
Cuts at: 5805 5805
Size: 6142
BspHI T'CATG_A
Cuts at: 2860 4031 5761 2860
Size: 1171 1730 3241
Fragments arranged by size:
3241 1730 1171
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2488 3497 844
Size: 1644 1009 3489
Fragments arranged by size:
3489 1644 1009
BsrBI CCG'CTC
Cuts at: 1394 1823 4029 1394
Size: 429 2206 3507
Fragments arranged by size:
3507 2206 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2950 4637 4811 65
Size: 1183 1702 1687 174 1396
Fragments arranged by size:
1702 1687 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4786 4973 1700
Size: 110 2976 187 2869
Fragments arranged by size:
2976 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6142
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6142
BssSI C'ACGA_G
Cuts at: 4202 5214 4202
Size: 1012 5130
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6142
BstBI TT'CG_AA
Cuts at: 4946 4946
Size: 6142
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6142
BstYI r'GATC_y
Cuts at: 1972 2271 2398 3472 3839 4224 4241 5693 5779
Size: 299 127 1074 367 385 17 1452 86
Cuts at: 5779 5791 6137 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 1074 385 367 346 299 127
86 17 12
Bsu36I CC'TnA_GG
Cuts at: 2316 2316
Size: 6142
BtgI C'CryG_G
Cuts at: 513 1065 2004 2631 513
Size: 552 939 627 4024
Fragments arranged by size:
4024 939 627 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5403
Fragments arranged by size:
5403 683 56
BtsI GCAGTG_nn'
Cuts at: 3658 4452 4472 3658
Size: 794 20 5328
Fragments arranged by size:
5328 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6142
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2932 2967 3100 3135 529
Size: 35 2368 35 133 35 3536
Fragments arranged by size:
3536 2368 133 35 35 35
DraI TTT'AAA
Cuts at: 3446 3446
Size: 6142
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5149 809
Size: 507 3833 1802
Fragments arranged by size:
3833 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3589 4478 6107
Size: 54 1425 50 275 18 1759 889 1629
Cuts at: 6107 8
Size: 43
Fragments arranged by size:
1759 1629 1425 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 3589 1537
Size: 275 1777 4090
Fragments arranged by size:
4090 1777 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2178 2593 4072 1353
Size: 825 415 1479 3423
Fragments arranged by size:
3423 1479 825 415
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4729 5103 5249 387
Size: 1064 138 3140 374 146 1280
Fragments arranged by size:
3140 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2227 2323 2227
Size: 96 6046
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2072 2297 2309 2312 2402 3027 3279
Size: 393 440 225 12 3 90 625 252
Cuts at: 3279 3367 4205 4802 5589 5857 1239
Size: 88 838 597 787 268 1524
Fragments arranged by size:
1524 838 787 625 597 440 393 268
252 225 90 88 12 3
EcoNI CCTnn'n_nnAGG
Cuts at: 2141 2315 2478 2881 2141
Size: 174 163 403 5402
Fragments arranged by size:
5402 403 174 163
EcoO109I rG'GnC_Cy
Cuts at: 2195 2195
Size: 6142
EcoRI G'AATT_C
Cuts at: 2997 3081 3249 3451 3829 2997
Size: 84 168 202 378 5310
Fragments arranged by size:
5310 378 202 168 84
EcoRV GAT'ATC
Cuts at: 3419 3419
Size: 6142
FspI TGC'GCA
Cuts at: 4648 4648
Size: 6142
HaeII r_GCGC'y
Cuts at: 1678 5289 1678
Size: 3611 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2068 2075 2100 2107 2458
Size: 32 135 32 496 7 25 7 351
Cuts at: 2458 2490 2794 2826 4783 4815 4857 4889 1373
Size: 32 304 32 1957 32 42 32 2626
Fragments arranged by size:
2626 1957 496 351 304 135 42 32
32 32 32 32 32 25 7 7
HincII GTy'rAC
Cuts at: 669 1657 1938 2234 3486 3732 669
Size: 988 281 296 1252 246 3079
Fragments arranged by size:
3079 1252 988 296 281 246
HindIII A'AGCT_T
Cuts at: 748 2570 3210 748
Size: 1822 640 3680
Fragments arranged by size:
3680 1822 640
HpaI GTT'AAC
Cuts at: 3732 3732
Size: 6142
KpnI G_GTAC'C
Cuts at: 3467 3467
Size: 6142
MfeI C'AATT_G
Cuts at: 3741 3741
Size: 6142
MluI A'CGCG_T
Cuts at: 6018 6018
Size: 6142
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3325 5256 5440 1305
Size: 2020 1931 184 2007
Fragments arranged by size:
2020 2007 1931 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4320
Fragments arranged by size:
4320 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2511 2865 2922 4100 4459 4618 518
Size: 648 1345 354 57 1178 359 159 2042
Fragments arranged by size:
2042 1345 1178 648 359 354 159 57
MspA1I CmG'CkG
Cuts at: 1606 2395 3558 4235 5383 5628 1606
Size: 789 1163 677 1148 245 2120
Fragments arranged by size:
2120 1163 1148 789 677 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 2631 513
Size: 552 939 627 4024
Fragments arranged by size:
4024 939 627 552
NdeI CA'TA_TG
Cuts at: 387 3284 387
Size: 2897 3245
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6142
NotI GC'GGCC_GC
Cuts at: 3589 3589
Size: 6142
NspI r_CATG'y
Cuts at: 1132 1229 2756 3329 3500 5045 1132
Size: 97 1527 573 171 1545 2229
Fragments arranged by size:
2229 1545 1527 573 171 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6142
PciI A'CATG_T
Cuts at: 5041 5041
Size: 6142
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2035 2737 1108
Size: 927 702 4513
Fragments arranged by size:
4513 927 702
PfoI T'CCnGG_A
Cuts at: 2220 2274 2220
Size: 54 6088
PmeI GTTT'AAAC
Cuts at: 3446 3446
Size: 6142
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4211 4243 5724 5756 4211
Size: 32 1481 32 4597
Fragments arranged by size:
4597 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2195 2195
Size: 6142
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6142
PsiI TTA'TAA
Cuts at: 3712 3712
Size: 6142
PstI C_TGCA'G
Cuts at: 830 3494 830
Size: 2664 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6142
PvuII CAG'CTG
Cuts at: 2395 2395
Size: 6142
SacI G_AGCT'C
Cuts at: 2229 2325 2229
Size: 96 6046
SalI G'TCGA_C
Cuts at: 1936 3484 1936
Size: 1548 4594
SbfI CC_TGCA'GG
Cuts at: 3494 3494
Size: 6142
ScaI AGT'ACT
Cuts at: 4390 6102 4390
Size: 1712 4430
SfcI C'TryA_G
Cuts at: 826 1045 2243 3490 4625 5306 5497 826
Size: 219 1198 1247 1135 681 191 1471
Fragments arranged by size:
1471 1247 1198 1135 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6142
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6142
SmaI CCC'GGG
Cuts at: 1872 2333 3469 1872
Size: 461 1136 4545
Fragments arranged by size:
4545 1136 461
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2199 4246 5147 5409 820
Size: 197 926 256 2047 901 262 1553
Fragments arranged by size:
2047 1553 926 901 262 256 197
SnaBI TAC'GTA
Cuts at: 493 3933 493
Size: 3440 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6142
SphI G_CATG'C
Cuts at: 1132 3500 1132
Size: 2368 3774
SspI AAT'ATT
Cuts at: 5 52 4066 5
Size: 47 4014 2081
Fragments arranged by size:
4014 2081 47
StuI AGG'CCT
Cuts at: 1517 2136 2979 3140 1517
Size: 619 843 161 4519
Fragments arranged by size:
4519 843 619 161
StyI C'CwwG_G
Cuts at: 513 1065 2004 2137 2262 2631 3581 513
Size: 552 939 133 125 369 950 3074
Fragments arranged by size:
3074 950 939 552 369 133 125
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2364 3167 4169 4186 4339 4524 4969
Size: 1255 784 803 1002 17 153 185 445
Cuts at: 4969 325
Size: 1498
Fragments arranged by size:
1498 1255 1002 803 784 445 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2485 2759 4388
Size: 274 80 33 51 1443 508 274 1629
Cuts at: 4388 6100 96
Size: 1712 138
Fragments arranged by size:
1712 1629 1443 508 274 274 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2138 2170 2406 2438 6102 6134 2138
Size: 32 236 32 3664 32 2146
Fragments arranged by size:
3664 2146 236 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6142
XbaI T'CTAG_A
Cuts at: 2837 3478 2837
Size: 641 5501
XhoI C'TCGA_G
Cuts at: 1943 2199 1943
Size: 256 5886
XmaI C'CCGG_G
Cuts at: 1870 2331 3467 1870
Size: 461 1136 4545
Fragments arranged by size:
4545 1136 461
XmnI GAAnn'nnTTC
Cuts at: 3296 4271 3296
Size: 975 5167
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3950 276
Size: 53 83 186 936 2416 2468
Fragments arranged by size:
2468 2416 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BcgI BciVI BclI
BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI
BstBI BstEII BstYI Bsu36I BtgI BtgZI BtsI ClaI
CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoNI EcoO109I EcoRI EcoRV FspI HaeII Hin4I
HincII HindIII HpaI KpnI MfeI MluI MmeI MscI
MslI MspA1I NcoI NdeI NheI NotI NspI PasI
PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI
PstI PvuI PvuII SacI SalI SbfI ScaI SfcI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstXI BstZ17I
DraIII FalI FseI FspAI KasI NaeI NarI NgoMIV
NruI NsiI PacI PmlI PspOMI PspXI PsrI RsrII
SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI
XcmI