Restriction map for
pFN21AB5487
(Circular) (Six-base) MAPSORT of: pFN21AB5487.seq Check: 520 from: 1 to: 5449
pFN21AB5487 5449 bp
With 182 enzymes: SgfI *
October 20, 2009 10:48 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3259 278
Size: 53 83 186 936 1723 2468
Fragments arranged by size:
2468 1723 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2574 2792 1937
Size: 637 218 4594
Fragments arranged by size:
4594 637 218
Acc65I G'GTAC_C
Cuts at: 2770 2770
Size: 5449
AclI AA'CG_TT
Cuts at: 1563 3576 3949 1563
Size: 2013 373 3063
Fragments arranged by size:
3063 2013 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2237 2714 3512 4896 5164 1632
Size: 605 477 798 1384 268 1917
Fragments arranged by size:
1917 1384 798 605 477 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5252
AflIII A'CryG_T
Cuts at: 1203 2022 4348 5325 1203
Size: 819 2326 977 1327
Fragments arranged by size:
2326 1327 977 819
AgeI A'CCGG_T
Cuts at: 4280 4280
Size: 5449
AhdI GACnn_n'nnGTC
Cuts at: 4178 4178
Size: 5449
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5449
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2453 1184
Size: 1269 4180
AlwNI CAG_nnn'CTG
Cuts at: 2342 2692 3192 4764 2342
Size: 350 500 1572 3027
Fragments arranged by size:
3027 1572 500 350
ApaLI G'TGCA_C
Cuts at: 2026 3506 4662 2026
Size: 1480 1156 2813
Fragments arranged by size:
2813 1480 1156
ApoI r'AATT_y
Cuts at: 1493 2758 2984 3136 5245 1493
Size: 1265 226 152 2109 1697
Fragments arranged by size:
2109 1697 1265 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4003 160
Size: 872 2971 1606
Fragments arranged by size:
2971 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5449
AvaI C'yCGr_G
Cuts at: 1870 1943 2636 2774 1870
Size: 73 693 138 4545
Fragments arranged by size:
4545 693 138 73
BamHI G'GATC_C
Cuts at: 2779 3146 2779
Size: 367 5082
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2084 2123 2429 2770 4225
Size: 325 217 627 297 39 306 341 1455
Cuts at: 4225 618
Size: 1842
Fragments arranged by size:
1842 1455 627 341 325 306 297 217
39
BanII G_rGCy'C
Cuts at: 1391 2768 1391
Size: 1377 4072
BbeI G_GCGC'C
Cuts at: 2088 2127 2088
Size: 39 5410
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4478
BbvCI CC'TCA_GC
Cuts at: 2496 2496
Size: 5449
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 3640 3674 1903
Size: 34 50 34 1619 34 3678
Fragments arranged by size:
3678 1619 50 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3341 4551 3341
Size: 1210 4239
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5449
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4060 136
Size: 107 122 71 631 605 2388 1525
Fragments arranged by size:
2388 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5444 5444
Size: 5449
BlpI GC'TnA_GC
Cuts at: 2866 2866
Size: 5449
Bme1580I G_kGCm'C
Cuts at: 1790 2030 2432 3510 4666 1790
Size: 240 402 1078 1156 2573
Fragments arranged by size:
2573 1156 1078 402 240
BmgBI CAC'GTC
Cuts at: 1318 2590 1318
Size: 1272 4177
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3180 4138 442
Size: 416 53 83 399 24 1763 958 1753
Fragments arranged by size:
1763 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 2516 1056
Size: 1460 3989
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2253 4109 1239
Size: 1014 1856 2579
Fragments arranged by size:
2579 1856 1014
Bpu10I CC'TnA_GC
Cuts at: 2297 2496 2297
Size: 199 5250
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3574 4439 4737 3574
Size: 865 298 4286
Fragments arranged by size:
4286 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4112 882
Size: 617 2613 2219
Fragments arranged by size:
2613 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2410 3240 493
Size: 711 1206 830 2702
Fragments arranged by size:
2702 1206 830 711
BsaBI GATnn'nnATC
Cuts at: 5443 5443
Size: 5449
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2085 2124 3256 3638
Size: 53 83 186 936 552 39 1132 382
Cuts at: 3638 4273 275
Size: 635 1451
Fragments arranged by size:
1451 1132 936 635 552 382 186 83
53 39
BsaWI w'CCGG_w
Cuts at: 2157 3882 4280 4554 4701 5112 2157
Size: 1725 398 274 147 411 2494
Fragments arranged by size:
2494 1725 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5224
Fragments arranged by size:
5224 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2713 1186
Size: 1527 3922
BseYI C'CCAG_C
Cuts at: 2331 4652 5148 2331
Size: 2321 496 2632
Fragments arranged by size:
2632 2321 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2432 1163
Size: 573 696 4180
Fragments arranged by size:
4180 696 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2095 2899 3660 4688 1540
Size: 275 186 94 804 761 1028 2301
Fragments arranged by size:
2301 1028 804 761 275 186 94
BsiHKAI G_wGCw'C
Cuts at: 2030 3510 3595 4666 2030
Size: 1480 85 1071 2813
Fragments arranged by size:
2813 1480 1071 85
BsmI GAATG_Cn'
Cuts at: 2325 2960 3053 2325
Size: 635 93 4721
Fragments arranged by size:
4721 635 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2030 2432 2768 3510 3595 4666 1391
Size: 399 240 402 336 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 402 399 336 240 85
BspEI T'CCGG_A
Cuts at: 5112 5112
Size: 5449
BspHI T'CATG_A
Cuts at: 3338 5068 3338
Size: 1730 3719
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2192 2804 844
Size: 1348 612 3489
Fragments arranged by size:
3489 1348 612
BsrBI CCG'CTC
Cuts at: 1394 1823 3336 1394
Size: 429 1513 3507
Fragments arranged by size:
3507 1513 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3944 4118 65
Size: 1183 2696 174 1396
Fragments arranged by size:
2696 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2090 4093 4280 1700
Size: 110 280 2003 187 2869
Fragments arranged by size:
2869 2003 280 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5449
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5449
BssSI C'ACGA_G
Cuts at: 3509 4521 3509
Size: 1012 4437
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5449
BstBI TT'CG_AA
Cuts at: 4253 4253
Size: 5449
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5449
BstXI CCAn_nnnn'nTGG
Cuts at: 2134 2134
Size: 5449
BstYI r'GATC_y
Cuts at: 1972 2779 3146 3531 3548 5000 5086 5098 5444
Size: 807 367 385 17 1452 86 12 346
Cuts at: 5444 1972
Size: 1977
Fragments arranged by size:
1977 1452 807 385 367 346 86 17
12
BtgI C'CryG_G
Cuts at: 513 1065 2061 2115 513
Size: 552 996 54 3847
Fragments arranged by size:
3847 996 552 54
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2017 487
Size: 683 56 791 3919
Fragments arranged by size:
3919 791 683 56
BtsI GCAGTG_nn'
Cuts at: 2311 2965 3759 3779 2311
Size: 654 794 20 3981
Fragments arranged by size:
3981 794 654 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5449
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5414
DraI TTT'AAA
Cuts at: 2753 2753
Size: 5449
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4456 809
Size: 507 3140 1802
Fragments arranged by size:
3140 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2092 2454 2896
Size: 54 1425 50 275 18 262 362 442
Cuts at: 2896 3785 5414 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 889 442 362 275 262 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2092 2896 1537
Size: 275 280 804 4090
Fragments arranged by size:
4090 804 280 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2256 2690 3379 1353
Size: 903 434 689 3423
Fragments arranged by size:
3423 903 689 434
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4036 4410 4556 387
Size: 1064 138 2447 374 146 1280
Fragments arranged by size:
2447 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2237 2253 2714 3512 4109 4896 5164
Size: 393 605 16 461 798 597 787 268
Cuts at: 5164 1239
Size: 1524
Fragments arranged by size:
1524 798 787 605 597 461 393 268
16
EcoO109I rG'GnC_Cy
Cuts at: 2689 2689
Size: 5449
EcoRI G'AATT_C
Cuts at: 2758 3136 2758
Size: 378 5071
FspI TGC'GCA
Cuts at: 3955 3955
Size: 5449
HaeII r_GCGC'y
Cuts at: 1678 2088 2127 2583 4596 1678
Size: 410 39 456 2013 2531
Fragments arranged by size:
2531 2013 456 410 39
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 4090 4122 4164 4196 1373
Size: 32 135 32 2518 32 42 32 2626
Fragments arranged by size:
2626 2518 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2491 2793 3039 669
Size: 988 281 553 302 246 3079
Fragments arranged by size:
3079 988 553 302 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5449
HpaI GTT'AAC
Cuts at: 3039 3039
Size: 5449
KasI G'GCGC_C
Cuts at: 2084 2123 2084
Size: 39 5410
KpnI G_GTAC'C
Cuts at: 2774 2774
Size: 5449
MfeI C'AATT_G
Cuts at: 3048 3048
Size: 5449
MluI A'CGCG_T
Cuts at: 5325 5325
Size: 5449
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2094 4563 4747 1305
Size: 789 2469 184 2007
Fragments arranged by size:
2469 2007 789 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2456 10
Size: 54 1425 343 624 3003
Fragments arranged by size:
3003 1425 624 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3407 3766 3925 518
Size: 648 2241 359 159 2042
Fragments arranged by size:
2241 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2063 2130 2314 2865 3542 4690 4935 1606
Size: 457 67 184 551 677 1148 245 2120
Fragments arranged by size:
2120 1148 677 551 457 245 184 67
NaeI GCC'GGC
Cuts at: 2092 2092
Size: 5449
NarI GG'CG_CC
Cuts at: 2085 2124 2085
Size: 39 5410
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 4897
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5449
NgoMIV G'CCGG_C
Cuts at: 2090 2090
Size: 5449
NheI G'CTAG_C
Cuts at: 1052 2512 1052
Size: 1460 3989
NotI GC'GGCC_GC
Cuts at: 2896 2896
Size: 5449
NspI r_CATG'y
Cuts at: 1132 1229 2026 2524 2686 2807 4352 1132
Size: 97 797 498 162 121 1545 2229
Fragments arranged by size:
2229 1545 797 498 162 121 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5449
PciI A'CATG_T
Cuts at: 2022 4348 2022
Size: 2326 3123
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5449
PmeI GTTT'AAAC
Cuts at: 2753 2753
Size: 5449
PmlI CAC'GTG
Cuts at: 2410 2410
Size: 5449
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3518 3550 5031 5063 3518
Size: 32 1481 32 3904
Fragments arranged by size:
3904 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2689 2689
Size: 5449
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5449
PsiI TTA'TAA
Cuts at: 2565 3019 2565
Size: 454 4995
PstI C_TGCA'G
Cuts at: 830 2306 2511 2801 830
Size: 1476 205 290 3478
Fragments arranged by size:
3478 1476 290 205
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5449
PvuII CAG'CTG
Cuts at: 2314 2314
Size: 5449
SacII CC_GC'GG
Cuts at: 2064 2064
Size: 5449
SalI G'TCGA_C
Cuts at: 1936 2791 1936
Size: 855 4594
SapI GCTCTTCn'nnn_
Cuts at: 2256 2256
Size: 5449
SbfI CC_TGCA'GG
Cuts at: 2801 2801
Size: 5449
ScaI AGT'ACT
Cuts at: 3697 5409 3697
Size: 1712 3737
SfcI C'TryA_G
Cuts at: 826 1045 2302 2507 2575 2797 3932 4613 4804
Size: 219 1257 205 68 222 1135 681 191
Cuts at: 4804 826
Size: 1471
Fragments arranged by size:
1471 1257 1135 681 222 219 205 191
68
SfoI GGC'GCC
Cuts at: 2086 2125 2086
Size: 39 5410
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5449
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5449
SmaI CCC'GGG
Cuts at: 1872 2776 1872
Size: 904 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3553 4454 4716 820
Size: 197 926 1610 901 262 1553
Fragments arranged by size:
1610 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3240 493
Size: 2747 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5449
SphI G_CATG'C
Cuts at: 1132 2524 2807 1132
Size: 1392 283 3774
Fragments arranged by size:
3774 1392 283
SspI AAT'ATT
Cuts at: 5 52 3373 5
Size: 47 3321 2081
Fragments arranged by size:
3321 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5449
StyI C'CwwG_G
Cuts at: 513 1065 2888 513
Size: 552 1823 3074
Fragments arranged by size:
3074 1823 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2692 3476 3493 3646 3831 4276 325
Size: 1255 1112 784 17 153 185 445 1498
Fragments arranged by size:
1498 1255 1112 784 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2630 3695 5407
Size: 274 80 33 51 1443 653 1065 1712
Cuts at: 5407 96
Size: 138
Fragments arranged by size:
1712 1443 1065 653 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2139 2171 5409 5441 2139
Size: 32 3238 32 2147
Fragments arranged by size:
3238 2147 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5449
XbaI T'CTAG_A
Cuts at: 2785 2785
Size: 5449
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5449
XmaI C'CCGG_G
Cuts at: 1870 2774 1870
Size: 904 4545
XmnI GAAnn'nnTTC
Cuts at: 3578 3578
Size: 5449
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3257 276
Size: 53 83 186 936 1723 2468
Fragments arranged by size:
2468 1723 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI
BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI
EarI EciI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I
HincII HindIII HpaI KasI KpnI MfeI MluI MmeI
MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV
NheI NotI NspI PasI PciI PflMI PmeI PmlI
PpiI PpuMI PshAI PsiI PstI PvuI PvuII SacII
SalI SapI SbfI ScaI SfcI SfoI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BfrBI
BplI BsiWI BsmBI BstZ17I Bsu36I DraIII EcoICRI EcoNI
EcoRV FalI FseI FspAI NruI NsiI PacI PfoI
PspOMI PspXI PsrI RsrII SacI SanDI SexAI SfiI
SrfI SwaI XcmI