Restriction map for
pFN21AB5486
(Circular) (Six-base) MAPSORT of: pFN21AB5486.seq Check: 2689 from: 1 to: 5284
pFN21AB5486 5284 bp
With 182 enzymes: SgfI *
January 16, 2009 15:25 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3094 278
Size: 53 83 186 936 1558 2468
Fragments arranged by size:
2468 1558 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2627 1937
Size: 690 4594
Acc65I G'GTAC_C
Cuts at: 2605 2605
Size: 5284
AclI AA'CG_TT
Cuts at: 1563 3411 3784 1563
Size: 1848 373 3063
Fragments arranged by size:
3063 1848 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2302 2446 3347 4731 4999 1632
Size: 670 144 901 1384 268 1917
Fragments arranged by size:
1917 1384 901 670 268 144
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5087
AflIII A'CryG_T
Cuts at: 1203 4183 5160 1203
Size: 2980 977 1327
Fragments arranged by size:
2980 1327 977
AgeI A'CCGG_T
Cuts at: 4115 4115
Size: 5284
AhdI GACnn_n'nnGTC
Cuts at: 4013 4013
Size: 5284
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5284
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2381 1184
Size: 1197 4087
AlwNI CAG_nnn'CTG
Cuts at: 2050 3027 4599 2050
Size: 977 1572 2735
Fragments arranged by size:
2735 1572 977
ApaLI G'TGCA_C
Cuts at: 3341 4497 3341
Size: 1156 4128
ApoI r'AATT_y
Cuts at: 1493 2507 2593 2819 2971 5080 1493
Size: 1014 86 226 152 2109 1697
Fragments arranged by size:
2109 1697 1014 226 152 86
AseI AT'TA_AT
Cuts at: 160 1032 3838 160
Size: 872 2806 1606
Fragments arranged by size:
2806 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5284
AvaI C'yCGr_G
Cuts at: 1870 1943 2033 2609 1870
Size: 73 90 576 4545
Fragments arranged by size:
4545 576 90 73
BamHI G'GATC_C
Cuts at: 2614 2981 2614
Size: 367 4917
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2237 2519 2605 4060 618
Size: 325 217 627 450 282 86 1455 1842
Fragments arranged by size:
1842 1455 627 450 325 282 217 86
BanII G_rGCy'C
Cuts at: 1391 2603 1391
Size: 1212 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2200 928
Size: 971 301 4012
Fragments arranged by size:
4012 971 301
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3475 3509 1903
Size: 34 1538 34 3678
Fragments arranged by size:
3678 1538 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3176 4386 3176
Size: 1210 4074
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5284
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2124 3895 136
Size: 107 122 71 631 605 452 1771 1525
Fragments arranged by size:
1771 1525 631 605 452 122 107 71
BglII A'GATC_T
Cuts at: 2154 2457 5279 2154
Size: 303 2822 2159
Fragments arranged by size:
2822 2159 303
BlpI GC'TnA_GC
Cuts at: 2270 2701 2270
Size: 431 4853
Bme1580I G_kGCm'C
Cuts at: 1790 3345 4501 1790
Size: 1555 1156 2573
Fragments arranged by size:
2573 1555 1156
BmgBI CAC'GTC
Cuts at: 1318 2206 1318
Size: 888 4396
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3015 3973 442
Size: 416 53 83 399 24 1598 958 1753
Fragments arranged by size:
1753 1598 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5284
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2315 2390 3944 1239
Size: 1076 75 1554 2579
Fragments arranged by size:
2579 1554 1076 75
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2093 2291 3409 4274 4572 2093
Size: 198 1118 865 298 2805
Fragments arranged by size:
2805 1118 865 298 198
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3947 882
Size: 617 2448 2219
Fragments arranged by size:
2448 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3075 493
Size: 711 1871 2702
Fragments arranged by size:
2702 1871 711
BsaBI GATnn'nnATC
Cuts at: 5278 5278
Size: 5284
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3091 3473 4108 275
Size: 53 83 186 936 1558 382 635 1451
Fragments arranged by size:
1558 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 3717 4115 4389 4536 4947 3717
Size: 398 274 147 411 4054
Fragments arranged by size:
4054 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2414 2444 1178
Size: 30 165 30 1011 30 4018
Fragments arranged by size:
4018 1011 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 5284
BseYI C'CCAG_C
Cuts at: 2564 4487 4983 2564
Size: 1923 496 2865
Fragments arranged by size:
2865 1923 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2399 1163
Size: 573 663 4048
Fragments arranged by size:
4048 663 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2734 3495 4523 1540
Size: 275 186 733 761 1028 2301
Fragments arranged by size:
2301 1028 761 733 275 186
BsiHKAI G_wGCw'C
Cuts at: 2205 3345 3430 4501 2205
Size: 1140 85 1071 2988
Fragments arranged by size:
2988 1140 1071 85
BsmI GAATG_Cn'
Cuts at: 2795 2888 2795
Size: 93 5191
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2205 2603 3345 3430 4501 1391
Size: 399 415 398 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 415 399 398 85
BspEI T'CCGG_A
Cuts at: 4947 4947
Size: 5284
BspHI T'CATG_A
Cuts at: 3173 4903 3173
Size: 1730 3554
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2058 2639 844
Size: 1214 581 3489
Fragments arranged by size:
3489 1214 581
BsrBI CCG'CTC
Cuts at: 1394 1823 3171 1394
Size: 429 1348 3507
Fragments arranged by size:
3507 1348 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2262 3779 3953 65
Size: 1183 1014 1517 174 1396
Fragments arranged by size:
1517 1396 1183 1014 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2123 3928 4115 1700
Size: 110 313 1805 187 2869
Fragments arranged by size:
2869 1805 313 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5284
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5284
BssSI C'ACGA_G
Cuts at: 2078 3344 4356 2078
Size: 1266 1012 3006
Fragments arranged by size:
3006 1266 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2050 794
Size: 1256 4028
BstBI TT'CG_AA
Cuts at: 4088 4088
Size: 5284
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5284
BstYI r'GATC_y
Cuts at: 1972 2154 2457 2614 2981 3366 3383 4835 4921
Size: 182 303 157 367 385 17 1452 86
Cuts at: 4921 4933 5279 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 385 367 346 303 182 157
86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2136 513
Size: 552 939 132 3661
Fragments arranged by size:
3661 939 552 132
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4545
Fragments arranged by size:
4545 683 56
BtsI GCAGTG_nn'
Cuts at: 2561 2575 2800 3594 3614 2561
Size: 14 225 794 20 4231
Fragments arranged by size:
4231 794 225 20 14
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5284
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5249
DraI TTT'AAA
Cuts at: 2588 2588
Size: 5284
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4291 809
Size: 507 2975 1802
Fragments arranged by size:
2975 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2125 2731 3620
Size: 54 1425 50 275 18 295 606 889
Cuts at: 3620 5249 8
Size: 1629 43
Fragments arranged by size:
1629 1425 889 606 295 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2731 1537
Size: 275 919 4090
Fragments arranged by size:
4090 919 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3214 1353
Size: 1861 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2534 3871 4245 4391 387
Size: 1064 138 945 1337 374 146 1280
Fragments arranged by size:
1337 1280 1064 945 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2302 2315 2390 2446 3347 3944 4731
Size: 393 670 13 75 56 901 597 787
Cuts at: 4731 4999 1239
Size: 268 1524
Fragments arranged by size:
1524 901 787 670 597 393 268 75
56 13
EcoO109I rG'GnC_Cy
Cuts at: 2047 2047
Size: 5284
EcoRI G'AATT_C
Cuts at: 2507 2593 2971 2507
Size: 86 378 4820
Fragments arranged by size:
4820 378 86
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2332 2332
Size: 5284
FspI TGC'GCA
Cuts at: 3790 3790
Size: 5284
HaeII r_GCGC'y
Cuts at: 1678 4431 1678
Size: 2753 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2414 2446 3925 3957 3999
Size: 32 135 32 842 32 1479 32 42
Cuts at: 3999 4031 1373
Size: 32 2626
Fragments arranged by size:
2626 1479 842 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2628 2874 669
Size: 988 281 690 246 3079
Fragments arranged by size:
3079 988 690 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5284
HpaI GTT'AAC
Cuts at: 2874 2874
Size: 5284
KpnI G_GTAC'C
Cuts at: 2609 2609
Size: 5284
MfeI C'AATT_G
Cuts at: 2883 2883
Size: 5284
MluI A'CGCG_T
Cuts at: 5160 5160
Size: 5284
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4398 4582 1305
Size: 3093 184 2007
Fragments arranged by size:
3093 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3462
Fragments arranged by size:
3462 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3242 3601 3760 518
Size: 648 2076 359 159 2042
Fragments arranged by size:
2076 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2700 3377 4525 4770 1606
Size: 1094 677 1148 245 2120
Fragments arranged by size:
2120 1148 1094 677 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 3793
Fragments arranged by size:
3793 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5284
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5284
NotI GC'GGCC_GC
Cuts at: 2731 2731
Size: 5284
NspI r_CATG'y
Cuts at: 1132 1229 2326 2642 4187 1132
Size: 97 1097 316 1545 2229
Fragments arranged by size:
2229 1545 1097 316 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5284
PciI A'CATG_T
Cuts at: 4183 4183
Size: 5284
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5284
PfoI T'CCnGG_A
Cuts at: 2250 2250
Size: 5284
PmeI GTTT'AAAC
Cuts at: 2588 2588
Size: 5284
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3353 3385 4866 4898 3353
Size: 32 1481 32 3739
Fragments arranged by size:
3739 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2047 2047
Size: 5284
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5284
PsiI TTA'TAA
Cuts at: 2854 2854
Size: 5284
PstI C_TGCA'G
Cuts at: 830 2046 2088 2556 2636 830
Size: 1216 42 468 80 3478
Fragments arranged by size:
3478 1216 468 80 42
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5284
SalI G'TCGA_C
Cuts at: 1936 2626 1936
Size: 690 4594
SbfI CC_TGCA'GG
Cuts at: 2636 2636
Size: 5284
ScaI AGT'ACT
Cuts at: 3532 5244 3532
Size: 1712 3572
SfcI C'TryA_G
Cuts at: 826 1045 2042 2084 2552 2632 3767 4448 4639
Size: 219 997 42 468 80 1135 681 191
Cuts at: 4639 826
Size: 1471
Fragments arranged by size:
1471 1135 997 681 468 219 191 80
42
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5284
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5284
SmaI CCC'GGG
Cuts at: 1872 2611 1872
Size: 739 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2108 2306 3388 4289 4551 820
Size: 197 926 165 198 1082 901 262 1553
Fragments arranged by size:
1553 1082 926 901 262 198 197 165
SnaBI TAC'GTA
Cuts at: 493 3075 493
Size: 2582 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5284
SphI G_CATG'C
Cuts at: 1132 2642 1132
Size: 1510 3774
SspI AAT'ATT
Cuts at: 5 52 3208 5
Size: 47 3156 2081
Fragments arranged by size:
3156 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5284
StyI C'CwwG_G
Cuts at: 513 1065 2004 2499 2723 513
Size: 552 939 495 224 3074
Fragments arranged by size:
3074 939 552 495 224
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3311 3328 3481 3666 4111 325
Size: 1255 1731 17 153 185 445 1498
Fragments arranged by size:
1731 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3530 5242 96
Size: 274 80 33 51 1443 1553 1712 138
Fragments arranged by size:
1712 1553 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5244 5276 5244
Size: 32 5252
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5284
XbaI T'CTAG_A
Cuts at: 2620 2620
Size: 5284
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2392 2392
Size: 5284
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5284
XmaI C'CCGG_G
Cuts at: 1870 2609 1870
Size: 739 4545
XmnI GAAnn'nnTTC
Cuts at: 3413 3413
Size: 5284
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3092 276
Size: 53 83 186 936 1558 2468
Fragments arranged by size:
2468 1558 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BcgI BciVI BclI
BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI
BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI
DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I
EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI
KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI
NdeI NheI NotI NspI PasI PciI PflMI PfoI
PmeI PpiI PpuMI PshAI PsiI PstI PvuI SalI
SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstXI BstZ17I
Bsu36I DraIII EcoICRI EcoNI EcoRV FseI FspAI KasI
NaeI NarI NgoMIV NruI NsiI PacI PmlI PspOMI
PspXI PsrI PvuII RsrII SacI SacII SanDI SapI
SexAI SfiI SfoI SrfI SwaI