Restriction map for
pFN21AB5485
(Circular) (Six-base) MAPSORT of: pFN21AB5485.seq Check: 1476 from: 1 to: 6256
pFN21AB5485 6256 bp
With 182 enzymes: SgfI *
February 27, 2009 14:34 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 3076 3076
Size: 6256
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4066 278
Size: 53 83 186 936 2530 2468
Fragments arranged by size:
2530 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3599 1937
Size: 1662 4594
Acc65I G'GTAC_C
Cuts at: 3577 3577
Size: 6256
AclI AA'CG_TT
Cuts at: 1563 4383 4756 1563
Size: 2820 373 3063
Fragments arranged by size:
3063 2820 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2232 2491 3299 3534 4319 5703 5971 1632
Size: 600 259 808 235 785 1384 268 1917
Fragments arranged by size:
1917 1384 808 785 600 268 259 235
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6059
AflIII A'CryG_T
Cuts at: 1203 5155 6132 1203
Size: 3952 977 1327
Fragments arranged by size:
3952 1327 977
AgeI A'CCGG_T
Cuts at: 5087 5087
Size: 6256
AhdI GACnn_n'nnGTC
Cuts at: 4985 4985
Size: 6256
AleI CACnn'nnGTG
Cuts at: 1166 3515 1166
Size: 2349 3907
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6256
AlwNI CAG_nnn'CTG
Cuts at: 3277 3999 5571 3277
Size: 722 1572 3962
Fragments arranged by size:
3962 1572 722
ApaI G_GGCC'C
Cuts at: 2034 2597 2034
Size: 563 5693
ApaLI G'TGCA_C
Cuts at: 4313 5469 4313
Size: 1156 5100
ApoI r'AATT_y
Cuts at: 1493 2560 3565 3791 3943 6052 1493
Size: 1067 1005 226 152 2109 1697
Fragments arranged by size:
2109 1697 1067 1005 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4810 160
Size: 872 3778 1606
Fragments arranged by size:
3778 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6256
AvaI C'yCGr_G
Cuts at: 1870 1943 2099 2253 3027 3039 3581 1870
Size: 73 156 154 774 12 542 4545
Fragments arranged by size:
4545 774 542 156 154 73 12
BamHI G'GATC_C
Cuts at: 3586 3953 3586
Size: 367 5889
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2283 3577 5032 618
Size: 325 217 627 496 1294 1455 1842
Fragments arranged by size:
1842 1455 1294 627 496 325 217
BanII G_rGCy'C
Cuts at: 1391 2034 2276 2597 2876 3439 3575 1391
Size: 643 242 321 279 563 136 4072
Fragments arranged by size:
4072 643 563 321 279 242 136
BbeI G_GCGC'C
Cuts at: 2287 2287
Size: 6256
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2098 3340 928
Size: 971 199 1242 3844
Fragments arranged by size:
3844 1242 971 199
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3220 3254 4447 4481 1903
Size: 34 1283 34 1193 34 3678
Fragments arranged by size:
3678 1283 1193 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4148 5358 4148
Size: 1210 5046
BclI T'GATC_A
Cuts at: 1548 2676 1548
Size: 1128 5128
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2072 2255 2459
Size: 107 122 71 631 605 400 183 204
Cuts at: 2459 3395 4867 136
Size: 936 1472 1525
Fragments arranged by size:
1525 1472 936 631 605 400 204 183
122 107 71
BglII A'GATC_T
Cuts at: 3252 6251 3252
Size: 2999 3257
BlpI GC'TnA_GC
Cuts at: 2637 3673 2637
Size: 1036 5220
Bme1580I G_kGCm'C
Cuts at: 1790 2034 2597 3404 4317 5473 1790
Size: 244 563 807 913 1156 2573
Fragments arranged by size:
2573 1156 913 807 563 244
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6256
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3433 3987 4945
Size: 416 53 83 399 24 2016 554 958
Cuts at: 4945 442
Size: 1753
Fragments arranged by size:
2016 1753 958 554 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6256
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2208 3377 4916 1239
Size: 969 1169 1539 2579
Fragments arranged by size:
2579 1539 1169 969
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2825 4381 5246 5544 2825
Size: 1556 865 298 3537
Fragments arranged by size:
3537 1556 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2366 4919 882
Size: 617 867 2553 2219
Fragments arranged by size:
2553 2219 867 617
BsaAI yAC'GTr
Cuts at: 493 1204 3327 4047 493
Size: 711 2123 720 2702
Fragments arranged by size:
2702 2123 720 711
BsaBI GATnn'nnATC
Cuts at: 3272 6250 3272
Size: 2978 3278
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2284 4063 4445 5080
Size: 53 83 186 936 751 1779 382 635
Cuts at: 5080 275
Size: 1451
Fragments arranged by size:
1779 1451 936 751 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 2349 2442 4689 5087 5361 5508 5919 2349
Size: 93 2247 398 274 147 411 2686
Fragments arranged by size:
2686 2247 411 398 274 147 93
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 6031
Fragments arranged by size:
6031 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 3263 3311 1186
Size: 2077 48 4131
Fragments arranged by size:
4131 2077 48
BseYI C'CCAG_C
Cuts at: 2269 2457 2590 2596 2738 2825 2860 5459 5955
Size: 188 133 6 142 87 35 2599 496
Cuts at: 5955 2269
Size: 2570
Fragments arranged by size:
2599 2570 496 188 142 133 87 35
6
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 3005 3242 1163
Size: 573 1269 237 4177
Fragments arranged by size:
4177 1269 573 237
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3706 4467 5495 1540
Size: 275 186 1705 761 1028 2301
Fragments arranged by size:
2301 1705 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 4317 4402 5473 4317
Size: 85 1071 5100
Fragments arranged by size:
5100 1071 85
BsiWI C'GTAC_G
Cuts at: 3327 3327
Size: 6256
BsmI GAATG_Cn'
Cuts at: 2780 3767 3860 2780
Size: 987 93 5176
Fragments arranged by size:
5176 987 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2034 2276 2597 2876 3404 3439 3575
Size: 399 244 242 321 279 528 35 136
Cuts at: 3575 4317 4402 5473 1391
Size: 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 528 399 321 279 244
242 136 85 35
BspEI T'CCGG_A
Cuts at: 5919 5919
Size: 6256
BspHI T'CATG_A
Cuts at: 4145 5875 4145
Size: 1730 4526
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3076 3611 844
Size: 2232 535 3489
Fragments arranged by size:
3489 2232 535
BsrBI CCG'CTC
Cuts at: 1394 1823 2344 2569 3016 4143 1394
Size: 429 521 225 447 1127 3507
Fragments arranged by size:
3507 1127 521 447 429 225
BsrDI GCAATG_nn'
Cuts at: 65 1248 2748 3171 4751 4925 65
Size: 1183 1500 423 1580 174 1396
Fragments arranged by size:
1580 1500 1396 1183 423 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2310 2453 2966 4900 5087 1700
Size: 110 500 143 513 1934 187 2869
Fragments arranged by size:
2869 1934 513 500 187 143 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6256
BssHII G'CGCG_C
Cuts at: 1925 2011 1925
Size: 86 6170
BssSI C'ACGA_G
Cuts at: 4316 5328 4316
Size: 1012 5244
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6256
BstBI TT'CG_AA
Cuts at: 5060 5060
Size: 6256
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6256
BstYI r'GATC_y
Cuts at: 1972 3111 3252 3273 3586 3953 4338 4355 5807
Size: 1139 141 21 313 367 385 17 1452
Cuts at: 5807 5893 5905 6251 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1452 1139 385 367 346 313 141
86 21 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2473 2555 2733 2913 3184 513
Size: 552 1408 82 178 180 271 3585
Fragments arranged by size:
3585 1408 552 271 180 178 82
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 3223 487
Size: 683 56 1997 3520
Fragments arranged by size:
3520 1997 683 56
BtsI GCAGTG_nn'
Cuts at: 2247 3772 4566 4586 2247
Size: 1525 794 20 3917
Fragments arranged by size:
3917 1525 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6256
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2782 2817 3193 3228 529
Size: 35 2218 35 376 35 3557
Fragments arranged by size:
3557 2218 376 35 35 35
DraI TTT'AAA
Cuts at: 3560 3560
Size: 6256
DraIII CAC_nnn'GTG
Cuts at: 3408 3408
Size: 6256
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5263 809
Size: 507 3947 1802
Fragments arranged by size:
3947 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2328 2667 2803
Size: 54 1425 50 275 18 498 339 136
Cuts at: 2803 3336 3475 3703 4592 6221 8
Size: 533 139 228 889 1629 43
Fragments arranged by size:
1629 1425 889 533 498 339 275 228
139 136 54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3703 1537
Size: 275 1891 4090
Fragments arranged by size:
4090 1891 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2864 4186 1353
Size: 1511 1322 3423
Fragments arranged by size:
3423 1511 1322
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4843 5217 5363 387
Size: 1064 138 3254 374 146 1280
Fragments arranged by size:
3254 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2208 2232 2491 3299 3377 3534 4319
Size: 393 576 24 259 808 78 157 785
Cuts at: 4319 4916 5703 5971 1239
Size: 597 787 268 1524
Fragments arranged by size:
1524 808 787 785 597 576 393 268
259 157 78 24
EcoNI CCTnn'n_nnAGG
Cuts at: 2678 3383 2678
Size: 705 5551
EcoO109I rG'GnC_Cy
Cuts at: 2031 3388 2031
Size: 1357 4899
EcoRI G'AATT_C
Cuts at: 3565 3943 3565
Size: 378 5878
FspI TGC'GCA
Cuts at: 4762 4762
Size: 6256
HaeII r_GCGC'y
Cuts at: 1678 2287 5403 1678
Size: 609 3116 2531
Fragments arranged by size:
3116 2531 609
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2181 2213 4897 4929 4971
Size: 32 135 32 609 32 2684 32 42
Cuts at: 4971 5003 1373
Size: 32 2626
Fragments arranged by size:
2684 2626 609 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2710 3415 3600 3846 669
Size: 988 281 772 705 185 246 3079
Fragments arranged by size:
3079 988 772 705 281 246 185
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6256
HpaI GTT'AAC
Cuts at: 3846 3846
Size: 6256
KasI G'GCGC_C
Cuts at: 2283 2283
Size: 6256
KpnI G_GTAC'C
Cuts at: 3581 3581
Size: 6256
MfeI C'AATT_G
Cuts at: 3855 3855
Size: 6256
MluI A'CGCG_T
Cuts at: 6132 6132
Size: 6256
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5370 5554 1305
Size: 4065 184 2007
Fragments arranged by size:
4065 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2669 10
Size: 54 1425 343 837 3597
Fragments arranged by size:
3597 1425 837 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2763 2885 3515 4214 4573 4732 518
Size: 648 1597 122 630 699 359 159 2042
Fragments arranged by size:
2042 1597 699 648 630 359 159 122
MspA1I CmG'CkG
Cuts at: 1606 2269 3344 3672 4349 5497 5742 1606
Size: 663 1075 328 677 1148 245 2120
Fragments arranged by size:
2120 1148 1075 677 663 328 245
NaeI GCC'GGC
Cuts at: 2312 2455 2968 2312
Size: 143 513 5600
Fragments arranged by size:
5600 513 143
NarI GG'CG_CC
Cuts at: 2284 2284
Size: 6256
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5704
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6256
NgoMIV G'CCGG_C
Cuts at: 2310 2453 2966 2310
Size: 143 513 5600
Fragments arranged by size:
5600 513 143
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6256
NotI GC'GGCC_GC
Cuts at: 3703 3703
Size: 6256
NspI r_CATG'y
Cuts at: 1132 1229 3614 5159 1132
Size: 97 2385 1545 2229
Fragments arranged by size:
2385 2229 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6256
PciI A'CATG_T
Cuts at: 5155 5155
Size: 6256
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6256
PfoI T'CCnGG_A
Cuts at: 2972 2972
Size: 6256
PmeI GTTT'AAAC
Cuts at: 3560 3560
Size: 6256
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4325 4357 5838 5870 4325
Size: 32 1481 32 4711
Fragments arranged by size:
4711 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6256
PsiI TTA'TAA
Cuts at: 3826 3826
Size: 6256
PspOMI G'GGCC_C
Cuts at: 2030 2593 2030
Size: 563 5693
PstI C_TGCA'G
Cuts at: 830 3504 3608 830
Size: 2674 104 3478
Fragments arranged by size:
3478 2674 104
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6256
PvuII CAG'CTG
Cuts at: 2269 3344 2269
Size: 1075 5181
SalI G'TCGA_C
Cuts at: 1936 3598 1936
Size: 1662 4594
SapI GCTCTTCn'nnn_
Cuts at: 2864 2864
Size: 6256
SbfI CC_TGCA'GG
Cuts at: 3608 3608
Size: 6256
ScaI AGT'ACT
Cuts at: 4504 6216 4504
Size: 1712 4544
SexAI A'CCwGG_T
Cuts at: 2409 2409
Size: 6256
SfcI C'TryA_G
Cuts at: 826 1045 3500 3604 4739 5420 5611 826
Size: 219 2455 104 1135 681 191 1471
Fragments arranged by size:
2455 1471 1135 681 219 191 104
SfiI GGCCn_nnn'nGGCC
Cuts at: 2255 2255
Size: 6256
SfoI GGC'GCC
Cuts at: 2285 2285
Size: 6256
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6256
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6256
SmaI CCC'GGG
Cuts at: 1872 2255 3029 3583 1872
Size: 383 774 554 4545
Fragments arranged by size:
4545 774 554 383
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2840 4360 5261 5523 820
Size: 197 926 897 1520 901 262 1553
Fragments arranged by size:
1553 1520 926 901 897 262 197
SnaBI TAC'GTA
Cuts at: 493 4047 493
Size: 3554 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6256
SphI G_CATG'C
Cuts at: 1132 3614 1132
Size: 2482 3774
SrfI GCCC'GGGC
Cuts at: 2255 3029 2255
Size: 774 5482
SspI AAT'ATT
Cuts at: 5 52 4180 5
Size: 47 4128 2081
Fragments arranged by size:
4128 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6256
StyI C'CwwG_G
Cuts at: 513 1065 2279 2367 2601 2853 3243 3695 513
Size: 552 1214 88 234 252 390 452 3074
Fragments arranged by size:
3074 1214 552 452 390 252 234 88
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2084 3086 4283 4300 4453 4638 5083
Size: 1255 504 1002 1197 17 153 185 445
Cuts at: 5083 325
Size: 1498
Fragments arranged by size:
1498 1255 1197 1002 504 445 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4502 6214 96
Size: 274 80 33 51 1443 2525 1712 138
Fragments arranged by size:
2525 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2161 2193 6216 6248 2161
Size: 32 4023 32 2169
Fragments arranged by size:
4023 2169 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 2426 1316
Size: 1110 5146
XbaI T'CTAG_A
Cuts at: 3592 3592
Size: 6256
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2822 3040 3331 3446 2822
Size: 218 291 115 5632
Fragments arranged by size:
5632 291 218 115
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6256
XmaI C'CCGG_G
Cuts at: 1870 2253 3027 3581 1870
Size: 383 774 554 4545
Fragments arranged by size:
4545 774 554 383
XmnI GAAnn'nnTTC
Cuts at: 2162 4385 2162
Size: 2223 4033
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4064 276
Size: 53 83 186 936 2530 2468
Fragments arranged by size:
2530 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsiWI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI
BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI
EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FspI
HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI
MluI MmeI MscI MslI MspA1I NaeI NarI NcoI
NdeI NgoMIV NheI NotI NspI PasI PciI PflMI
PfoI PmeI PpiI PshAI PsiI PspOMI PstI PvuI
PvuII SalI SapI SbfI ScaI SexAI SfcI SfiI
SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SrfI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI AscI AvrII BaeI BbvCI BfrBI BplI
Bpu10I BsmBI BstXI BstZ17I Bsu36I EcoICRI EcoRV FalI
FseI FspAI NruI NsiI PacI PmlI PpuMI PspXI
PsrI RsrII SacI SacII SanDI SwaI