Restriction map for
pFN21AB5484
(Circular) (Six-base) MAPSORT of: pFN21AB5484.seq Check: 3987 from: 1 to: 6655
pFN21AB5484 6655 bp
With 182 enzymes: SgfI *
October 20, 2009 10:35 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 3085 3085
Size: 6655
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4465 278
Size: 53 83 186 936 2929 2468
Fragments arranged by size:
2929 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3998 1937
Size: 2061 4594
Acc65I G'GTAC_C
Cuts at: 3976 3976
Size: 6655
AclI AA'CG_TT
Cuts at: 1563 4782 5155 1563
Size: 3219 373 3063
Fragments arranged by size:
3219 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2484 2814 4718 6102 6370 1632
Size: 852 330 1904 1384 268 1917
Fragments arranged by size:
1917 1904 1384 852 330 268
AfeI AGC'GCT
Cuts at: 2243 2357 2243
Size: 114 6541
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6458
AflIII A'CryG_T
Cuts at: 1203 2320 5554 6531 1203
Size: 1117 3234 977 1327
Fragments arranged by size:
3234 1327 1117 977
AgeI A'CCGG_T
Cuts at: 5486 5486
Size: 6655
AhdI GACnn_n'nnGTC
Cuts at: 3016 5384 3016
Size: 2368 4287
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6655
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6655
AlwNI CAG_nnn'CTG
Cuts at: 3052 3085 3118 3394 3433 4398 5970 3052
Size: 33 33 276 39 965 1572 3737
Fragments arranged by size:
3737 1572 965 276 39 33 33
ApaI G_GGCC'C
Cuts at: 2013 2013
Size: 6655
ApaLI G'TGCA_C
Cuts at: 4712 5868 4712
Size: 1156 5499
ApoI r'AATT_y
Cuts at: 1493 2435 3964 4190 4342 6451 1493
Size: 942 1529 226 152 2109 1697
Fragments arranged by size:
2109 1697 1529 942 226 152
AseI AT'TA_AT
Cuts at: 160 1032 2979 5209 160
Size: 872 1947 2230 1606
Fragments arranged by size:
2230 1947 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6655
AvaI C'yCGr_G
Cuts at: 1870 1943 2047 2155 2575 2701 2866 3320 3980
Size: 73 104 108 420 126 165 454 660
Cuts at: 3980 1870
Size: 4545
Fragments arranged by size:
4545 660 454 420 165 126 108 104
73
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 2282 2315 2282
Size: 33 6622
BamHI G'GATC_C
Cuts at: 3316 3985 4352 3316
Size: 669 367 5619
Fragments arranged by size:
5619 669 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2187 2458 3715 3976 5431
Size: 325 217 627 400 271 1257 261 1455
Cuts at: 5431 618
Size: 1842
Fragments arranged by size:
1842 1455 1257 627 400 325 271 261
217
BanII G_rGCy'C
Cuts at: 1391 2013 2138 3974 1391
Size: 622 125 1836 4072
Fragments arranged by size:
4072 1836 622 125
BbeI G_GCGC'C
Cuts at: 2191 2191
Size: 6655
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 5684
BbvCI CC'TCA_GC
Cuts at: 2724 2724
Size: 6655
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4846 4880 1903
Size: 34 2909 34 3678
Fragments arranged by size:
3678 2909 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4547 5757 4547
Size: 1210 5445
BclI T'GATC_A
Cuts at: 1548 3762 1548
Size: 2214 4441
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2049 2171 3203
Size: 107 122 71 631 605 377 122 1032
Cuts at: 3203 5266 136
Size: 2063 1525
Fragments arranged by size:
2063 1525 1032 631 605 377 122 122
107 71
BglII A'GATC_T
Cuts at: 6650 6650
Size: 6655
BlpI GC'TnA_GC
Cuts at: 4072 4072
Size: 6655
Bme1580I G_kGCm'C
Cuts at: 1790 2013 2415 4716 5872 1790
Size: 223 402 2301 1156 2573
Fragments arranged by size:
2573 2301 1156 402 223
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6655
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3474 4386 5344
Size: 416 53 83 399 24 2057 912 958
Cuts at: 5344 442
Size: 1753
Fragments arranged by size:
2057 1753 958 912 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6655
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3803 3803
Size: 6655
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2300 2625 2975 3107 3525 3899 3962 5315
Size: 1061 325 350 132 418 374 63 1353
Cuts at: 5315 1239
Size: 2579
Fragments arranged by size:
2579 1353 1061 418 374 350 325 132
63
Bpu10I CC'TnA_GC
Cuts at: 2724 3426 2724
Size: 702 5953
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4780 5645 5943 4780
Size: 865 298 5492
Fragments arranged by size:
5492 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 5318 882
Size: 617 3819 2219
Fragments arranged by size:
3819 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2321 4446 493
Size: 711 1117 2125 2702
Fragments arranged by size:
2702 2125 1117 711
BsaBI GATnn'nnATC
Cuts at: 6649 6649
Size: 6655
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2188 4462 4844 5479
Size: 53 83 186 936 655 2274 382 635
Cuts at: 5479 275
Size: 1451
Fragments arranged by size:
2274 1451 936 655 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 5088 5486 5760 5907 6318 5088
Size: 398 274 147 411 5425
Fragments arranged by size:
5425 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2241 2271 2345 2375 2612
Size: 30 165 30 838 30 74 30 237
Cuts at: 2612 2642 3083 3113 1178
Size: 30 441 30 4720
Fragments arranged by size:
4720 838 441 237 165 74 30 30
30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2753 2972 3104 3389 3446 3896 1186
Size: 1567 219 132 285 57 450 3945
Fragments arranged by size:
3945 1567 450 285 219 132 57
BseYI C'CCAG_C
Cuts at: 2223 3722 5858 6354 2223
Size: 1499 2136 496 2524
Fragments arranged by size:
2524 2136 1499 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2552 1163
Size: 573 816 5266
Fragments arranged by size:
5266 816 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2148 2221 4105 4866 5894 1540
Size: 275 186 147 73 1884 761 1028 2301
Fragments arranged by size:
2301 1884 1028 761 275 186 147 73
BsiHKAI G_wGCw'C
Cuts at: 2925 3063 4716 4801 5872 2925
Size: 138 1653 85 1071 3708
Fragments arranged by size:
3708 1653 1071 138 85
BsmI GAATG_Cn'
Cuts at: 3661 4166 4259 3661
Size: 505 93 6057
Fragments arranged by size:
6057 505 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2013 2138 2415 2925 3063 3974 4716
Size: 399 223 125 277 510 138 911 742
Cuts at: 4716 4801 5872 1391
Size: 85 1071 2174
Fragments arranged by size:
2174 1071 911 742 510 399 277 223
138 125 85
BspEI T'CCGG_A
Cuts at: 6318 6318
Size: 6655
BspHI T'CATG_A
Cuts at: 4544 6274 4544
Size: 1730 4925
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2217 3036 3085 3261 4010 844
Size: 1373 819 49 176 749 3489
Fragments arranged by size:
3489 1373 819 749 176 49
BsrBI CCG'CTC
Cuts at: 1394 1823 2379 3773 4542 1394
Size: 429 556 1394 769 3507
Fragments arranged by size:
3507 1394 769 556 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 5150 5324 65
Size: 1183 3902 174 1396
Fragments arranged by size:
3902 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2126 2216 2472 5299 5486 1700
Size: 110 316 90 256 2827 187 2869
Fragments arranged by size:
2869 2827 316 256 187 110 90
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6655
BssHII G'CGCG_C
Cuts at: 1925 2098 2100 1925
Size: 173 2 6480
Fragments arranged by size:
6480 173 2
BssSI C'ACGA_G
Cuts at: 2924 4715 5727 2924
Size: 1791 1012 3852
Fragments arranged by size:
3852 1791 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2275 3286 794
Size: 1481 1011 4163
Fragments arranged by size:
4163 1481 1011
BstBI TT'CG_AA
Cuts at: 5459 5459
Size: 6655
BstEII G'GTnAC_C
Cuts at: 1184 2675 1184
Size: 1491 5164
BstXI CCAn_nnnn'nTGG
Cuts at: 2174 2350 3487 2174
Size: 176 1137 5342
Fragments arranged by size:
5342 1137 176
BstYI r'GATC_y
Cuts at: 1972 3208 3316 3985 4352 4737 4754 6206 6292
Size: 1236 108 669 367 385 17 1452 86
Cuts at: 6292 6304 6650 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 1236 669 385 367 346 108
86 17 12
Bsu36I CC'TnA_GG
Cuts at: 2936 3216 2936
Size: 280 6375
BtgI C'CryG_G
Cuts at: 513 1065 2004 2349 2611 3003 3312 3844 513
Size: 552 939 345 262 392 309 532 3324
Fragments arranged by size:
3324 939 552 532 392 345 309 262
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 3130 487
Size: 683 56 1904 4012
Fragments arranged by size:
4012 1904 683 56
BtsI GCAGTG_nn'
Cuts at: 2847 4171 4965 4985 2847
Size: 1324 794 20 4517
Fragments arranged by size:
4517 1324 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6655
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6620
DraI TTT'AAA
Cuts at: 3959 3959
Size: 6655
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 3733 5662 809
Size: 507 2417 1929 1802
Fragments arranged by size:
2417 1929 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2124 2145 2218
Size: 54 1425 50 275 18 294 21 73
Cuts at: 2218 3306 4102 4991 6620 8
Size: 1088 796 889 1629 43
Fragments arranged by size:
1629 1425 1088 889 796 294 275 73
54 50 43 21 18
EagI C'GGCC_G
Cuts at: 1537 1812 2145 2218 4102 1537
Size: 275 333 73 1884 4090
Fragments arranged by size:
4090 1884 333 275 73
EarI CTCTTCn'nnn_
Cuts at: 1353 2730 2790 2796 4585 1353
Size: 1377 60 6 1789 3423
Fragments arranged by size:
3423 1789 1377 60 6
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3201 3236 3792 5242 5616 5762
Size: 1064 138 1612 35 556 1450 374 146
Cuts at: 5762 387
Size: 1280
Fragments arranged by size:
1612 1450 1280 1064 556 374 146 138
35
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2300 2484 2625 2814 2975 3107 3525
Size: 393 668 184 141 189 161 132 418
Cuts at: 3525 3899 3962 4718 5315 6102 6370 1239
Size: 374 63 756 597 787 268 1524
Fragments arranged by size:
1524 787 756 668 597 418 393 374
268 189 184 161 141 132 63
EcoNI CCTnn'n_nnAGG
Cuts at: 3425 3425
Size: 6655
EcoO109I rG'GnC_Cy
Cuts at: 2009 2152 2206 2263 2449 2557 2646 3049 3476
Size: 143 54 57 186 108 89 403 427
Cuts at: 3476 2009
Size: 5188
Fragments arranged by size:
5188 427 403 186 143 108 89 57
54
EcoRI G'AATT_C
Cuts at: 3964 4342 3964
Size: 378 6277
FspI TGC'GCA
Cuts at: 2213 3815 5161 2213
Size: 1602 1346 3707
Fragments arranged by size:
3707 1602 1346
HaeII r_GCGC'y
Cuts at: 1678 2191 2245 2359 3118 3169 3250 5802 1678
Size: 513 54 114 759 51 81 2552 2531
Fragments arranged by size:
2552 2531 759 513 114 81 54 51
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2241 2273 2729 2761 3002
Size: 32 135 32 669 32 456 32 241
Cuts at: 3002 3034 3083 3115 3370 3402 3587 3619 3734
Size: 32 49 32 255 32 185 32 115
Cuts at: 3734 3766 5296 5328 5370 5402 1373
Size: 32 1530 32 42 32 2626
Fragments arranged by size:
2626 1530 669 456 255 241 185 135
115 49 42 32 32 32 32 32
32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3999 4245 669
Size: 988 281 2061 246 3079
Fragments arranged by size:
3079 2061 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6655
HpaI GTT'AAC
Cuts at: 4245 4245
Size: 6655
KasI G'GCGC_C
Cuts at: 2187 2187
Size: 6655
KpnI G_GTAC'C
Cuts at: 3980 3980
Size: 6655
MfeI C'AATT_G
Cuts at: 4254 4254
Size: 6655
MluI A'CGCG_T
Cuts at: 6531 6531
Size: 6655
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5769 5953 1305
Size: 4464 184 2007
Fragments arranged by size:
4464 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4833
Fragments arranged by size:
4833 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2348 3563 3915 4613 4972 5131 518
Size: 648 1182 1215 352 698 359 159 2042
Fragments arranged by size:
2042 1215 1182 698 648 359 352 159
MspA1I CmG'CkG
Cuts at: 1606 2024 2062 2068 2223 2385 2421 3631 3722
Size: 418 38 6 155 162 36 1210 91
Cuts at: 3722 3907 4071 4748 5896 6141 1606
Size: 185 164 677 1148 245 2120
Fragments arranged by size:
2120 1210 1148 677 418 245 185 164
162 155 91 38 36 6
NarI GG'CG_CC
Cuts at: 2188 2188
Size: 6655
NcoI C'CATG_G
Cuts at: 513 1065 2004 2349 3003 3844 513
Size: 552 939 345 654 841 3324
Fragments arranged by size:
3324 939 841 654 552 345
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6655
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6655
NotI GC'GGCC_GC
Cuts at: 4102 4102
Size: 6655
NspI r_CATG'y
Cuts at: 1132 1229 2584 2905 3305 4013 5558 1132
Size: 97 1355 321 400 708 1545 2229
Fragments arranged by size:
2229 1545 1355 708 400 321 97
PasI CC'CwG_GG
Cuts at: 1348 2554 3503 1348
Size: 1206 949 4500
Fragments arranged by size:
4500 1206 949
PciI A'CATG_T
Cuts at: 5554 5554
Size: 6655
PflMI CCAn_nnn'nTGG
Cuts at: 1108 3304 3850 1108
Size: 2196 546 3913
Fragments arranged by size:
3913 2196 546
PfoI T'CCnGG_A
Cuts at: 2259 2372 3447 3939 2259
Size: 113 1075 492 4975
Fragments arranged by size:
4975 1075 492 113
PmeI GTTT'AAAC
Cuts at: 3959 3959
Size: 6655
PmlI CAC'GTG
Cuts at: 2321 2321
Size: 6655
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4724 4756 6237 6269 4724
Size: 32 1481 32 5110
Fragments arranged by size:
5110 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2206 2263 2646 3476 2206
Size: 57 383 830 5385
Fragments arranged by size:
5385 830 383 57
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6655
PsiI TTA'TAA
Cuts at: 4225 4225
Size: 6655
PspOMI G'GGCC_C
Cuts at: 2009 2009
Size: 6655
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 3914 3946 3914
Size: 32 6623
PstI C_TGCA'G
Cuts at: 830 2291 2313 2549 3081 3204 3537 3906 4007
Size: 1461 22 236 532 123 333 369 101
Cuts at: 4007 830
Size: 3478
Fragments arranged by size:
3478 1461 532 369 333 236 123 101
22
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6655
PvuII CAG'CTG
Cuts at: 2385 3722 2385
Size: 1337 5318
SalI G'TCGA_C
Cuts at: 1936 3997 1936
Size: 2061 4594
SanDI GG'GwC_CC
Cuts at: 2263 2646 3476 2263
Size: 383 830 5442
Fragments arranged by size:
5442 830 383
SbfI CC_TGCA'GG
Cuts at: 3204 4007 3204
Size: 803 5852
ScaI AGT'ACT
Cuts at: 2285 4903 6615 2285
Size: 2618 1712 2325
Fragments arranged by size:
2618 2325 1712
SexAI A'CCwGG_T
Cuts at: 2403 2403
Size: 6655
SfcI C'TryA_G
Cuts at: 826 1045 2287 2309 2545 3077 3200 3533 3902
Size: 219 1242 22 236 532 123 333 369
Cuts at: 3902 4003 5138 5819 6010 826
Size: 101 1135 681 191 1471
Fragments arranged by size:
1471 1242 1135 681 532 369 333 236
219 191 123 101 22
SfoI GGC'GCC
Cuts at: 2189 2189
Size: 6655
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6655
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6655
SmaI CCC'GGG
Cuts at: 1872 2049 2157 2703 3982 1872
Size: 177 108 546 1279 4545
Fragments arranged by size:
4545 1279 546 177 108
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4759 5660 5922 820
Size: 197 926 2816 901 262 1553
Fragments arranged by size:
2816 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 4446 493
Size: 3953 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6655
SphI G_CATG'C
Cuts at: 1132 2584 2905 4013 1132
Size: 1452 321 1108 3774
Fragments arranged by size:
3774 1452 1108 321
SrfI GCCC'GGGC
Cuts at: 2049 2049
Size: 6655
SspI AAT'ATT
Cuts at: 5 52 4579 5
Size: 47 4527 2081
Fragments arranged by size:
4527 2081 47
StuI AGG'CCT
Cuts at: 1517 2543 1517
Size: 1026 5629
StyI C'CwwG_G
Cuts at: 513 1065 2004 2349 3003 3177 3549 3668 3844
Size: 552 939 345 654 174 372 119 176
Cuts at: 3844 4094 513
Size: 250 3074
Fragments arranged by size:
3074 939 654 552 372 345 250 176
174 119
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 4682 4699 4852 5037 5482 325
Size: 1255 3102 17 153 185 445 1498
Fragments arranged by size:
3102 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2283 4901 6613
Size: 274 80 33 51 1443 306 2618 1712
Cuts at: 6613 96
Size: 138
Fragments arranged by size:
2618 1712 1443 306 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2345 2377 2612 2644 3053 3085 6615 6647 2345
Size: 32 235 32 409 32 3530 32 2353
Fragments arranged by size:
3530 2353 409 235 32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 2253 1316
Size: 937 5718
XbaI T'CTAG_A
Cuts at: 3991 3991
Size: 6655
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2119 2881 2950 3874 2119
Size: 762 69 924 4900
Fragments arranged by size:
4900 924 762 69
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6655
XmaI C'CCGG_G
Cuts at: 1870 2047 2155 2701 3980 1870
Size: 177 108 546 1279 4545
Fragments arranged by size:
4545 1279 546 177 108
XmnI GAAnn'nnTTC
Cuts at: 4784 4784
Size: 6655
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4463 276
Size: 53 83 186 936 2929 2468
Fragments arranged by size:
2929 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII
BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII
BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI
BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI
ClaI CspCI DraI DrdI EaeI EagI EarI EciI
Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII
HindIII HpaI KasI KpnI MfeI MluI MmeI MscI
MslI MspA1I NarI NcoI NdeI NheI NotI NspI
PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI
PshAI PsiI PspOMI PsrI PstI PvuI PvuII SalI
SanDI SbfI ScaI SexAI SfcI SfoI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SrfI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AloI AscI AvrII BfrBI BsiWI BsmBI BstZ17I DraIII
EcoICRI EcoRV FalI FseI FspAI NaeI NgoMIV NruI
NsiI PacI PspXI RsrII SacI SacII SapI SfiI
SwaI