Restriction map for
pFN21AB5481
(Circular) (Six-base) MAPSORT of: pFN21AB5481.seq Check: 1707 from: 1 to: 6064
pFN21AB5481 6064 bp
With 182 enzymes: SgfI *
April 15, 2010 09:33 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3874 278
Size: 53 83 186 936 2338 2468
Fragments arranged by size:
2468 2338 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3407 1937
Size: 1470 4594
Acc65I G'GTAC_C
Cuts at: 3385 3385
Size: 6064
AclI AA'CG_TT
Cuts at: 1563 4191 4564 1563
Size: 2628 373 3063
Fragments arranged by size:
3063 2628 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2209 2450 2855 3227 4127 5511 5779 1632
Size: 577 241 405 372 900 1384 268 1917
Fragments arranged by size:
1917 1384 900 577 405 372 268 241
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5867
AflIII A'CryG_T
Cuts at: 1203 2896 4963 5940 1203
Size: 1693 2067 977 1327
Fragments arranged by size:
2067 1693 1327 977
AgeI A'CCGG_T
Cuts at: 4895 4895
Size: 6064
AhdI GACnn_n'nnGTC
Cuts at: 4793 4793
Size: 6064
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6064
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6064
AlwNI CAG_nnn'CTG
Cuts at: 3002 3044 3807 5379 3002
Size: 42 763 1572 3687
Fragments arranged by size:
3687 1572 763 42
ApaLI G'TGCA_C
Cuts at: 4121 5277 4121
Size: 1156 4908
ApoI r'AATT_y
Cuts at: 1493 2438 2530 3331 3373 3599 3751 5860 1493
Size: 945 92 801 42 226 152 2109 1697
Fragments arranged by size:
2109 1697 945 801 226 152 92 42
AseI AT'TA_AT
Cuts at: 160 1032 4618 160
Size: 872 3586 1606
Fragments arranged by size:
3586 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6064
AvaI C'yCGr_G
Cuts at: 1870 1943 2035 3389 1870
Size: 73 92 1354 4545
Fragments arranged by size:
4545 1354 92 73
BamHI G'GATC_C
Cuts at: 3394 3761 3394
Size: 367 5697
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3385 4840 618
Size: 325 217 627 1598 1455 1842
Fragments arranged by size:
1842 1598 1455 627 325 217
BanII G_rGCy'C
Cuts at: 1391 3383 1391
Size: 1992 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3159 928
Size: 971 1260 3833
Fragments arranged by size:
3833 1260 971
BbvCI CC'TCA_GC
Cuts at: 2472 2472
Size: 6064
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4255 4289 1903
Size: 34 2318 34 3678
Fragments arranged by size:
3678 2318 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3140 3956 5166 3140
Size: 816 1210 4038
Fragments arranged by size:
4038 1210 816
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6064
BfrBI ATG'CAT
Cuts at: 2858 2858
Size: 6064
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4675 136
Size: 107 122 71 631 605 3003 1525
Fragments arranged by size:
3003 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6059 6059
Size: 6064
BlpI GC'TnA_GC
Cuts at: 3481 3481
Size: 6064
Bme1580I G_kGCm'C
Cuts at: 1790 4125 5281 1790
Size: 2335 1156 2573
Fragments arranged by size:
2573 2335 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6064
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3795 4753 442
Size: 416 53 83 399 24 2378 958 1753
Fragments arranged by size:
2378 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6064
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 3066 4724 1239
Size: 1827 1658 2579
Fragments arranged by size:
2579 1827 1658
Bpu10I CC'TnA_GC
Cuts at: 2472 2472
Size: 6064
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2138 3075 4189 5054 5352 2138
Size: 937 1114 865 298 2850
Fragments arranged by size:
2850 1114 937 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4727 882
Size: 617 3228 2219
Fragments arranged by size:
3228 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3855 493
Size: 711 2651 2702
Fragments arranged by size:
2702 2651 711
BsaBI GATnn'nnATC
Cuts at: 6058 6058
Size: 6064
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3871 4253 4888 275
Size: 53 83 186 936 2338 382 635 1451
Fragments arranged by size:
2338 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 2550 4497 4895 5169 5316 5727 2550
Size: 1947 398 274 147 411 2887
Fragments arranged by size:
2887 1947 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5839
Fragments arranged by size:
5839 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2627 1186
Size: 1441 4623
BseYI C'CCAG_C
Cuts at: 2888 5267 5763 2888
Size: 2379 496 3189
Fragments arranged by size:
3189 2379 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2865 2915 1163
Size: 573 1129 50 4312
Fragments arranged by size:
4312 1129 573 50
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3514 4275 5303 1540
Size: 275 186 1513 761 1028 2301
Fragments arranged by size:
2301 1513 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2098 4125 4210 5281 2098
Size: 2027 85 1071 2881
Fragments arranged by size:
2881 2027 1071 85
BsmI GAATG_Cn'
Cuts at: 2381 2560 3575 3668 2381
Size: 179 1015 93 4777
Fragments arranged by size:
4777 1015 179 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2098 3383 4125 4210 5281 1391
Size: 399 308 1285 742 85 1071 2174
Fragments arranged by size:
2174 1285 1071 742 399 308 85
BspEI T'CCGG_A
Cuts at: 5727 5727
Size: 6064
BspHI T'CATG_A
Cuts at: 3953 5683 3953
Size: 1730 4334
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3419 844
Size: 2575 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3951 1394
Size: 429 2128 3507
Fragments arranged by size:
3507 2128 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4559 4733 65
Size: 1183 3311 174 1396
Fragments arranged by size:
3311 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2024 4708 4895 1700
Size: 110 214 2684 187 2869
Fragments arranged by size:
2869 2684 214 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6064
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6064
BssSI C'ACGA_G
Cuts at: 4124 5136 4124
Size: 1012 5052
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2828 2984 794
Size: 2034 156 3874
Fragments arranged by size:
3874 2034 156
BstBI TT'CG_AA
Cuts at: 4868 4868
Size: 6064
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6064
BstYI r'GATC_y
Cuts at: 1972 3394 3761 4146 4163 5615 5701 5713 6059
Size: 1422 367 385 17 1452 86 12 346
Cuts at: 6059 1972
Size: 1977
Fragments arranged by size:
1977 1452 1422 385 367 346 86 17
12
Bsu36I CC'TnA_GG
Cuts at: 2109 2109
Size: 6064
BtgI C'CryG_G
Cuts at: 513 1065 2305 2764 513
Size: 552 1240 459 3813
Fragments arranged by size:
3813 1240 552 459
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5325
Fragments arranged by size:
5325 683 56
BtsI GCAGTG_nn'
Cuts at: 3580 4374 4394 3580
Size: 794 20 5250
Fragments arranged by size:
5250 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6064
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2746 2781 529
Size: 35 2182 35 3812
Fragments arranged by size:
3812 2182 35 35
DraI TTT'AAA
Cuts at: 3368 3368
Size: 6064
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2801 5071 809
Size: 507 1485 2270 1802
Fragments arranged by size:
2270 1802 1485 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3304 3511 4400
Size: 54 1425 50 275 18 1474 207 889
Cuts at: 4400 6029 8
Size: 1629 43
Fragments arranged by size:
1629 1474 1425 889 275 207 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3511 1537
Size: 275 1699 4090
Fragments arranged by size:
4090 1699 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3994 1353
Size: 2641 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4651 5025 5171 387
Size: 1064 138 3062 374 146 1280
Fragments arranged by size:
3062 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2209 2450 2855 3066 3227 4127 4724
Size: 393 577 241 405 211 161 900 597
Cuts at: 4724 5511 5779 1239
Size: 787 268 1524
Fragments arranged by size:
1524 900 787 597 577 405 393 268
241 211 161
EcoO109I rG'GnC_Cy
Cuts at: 2113 3195 2113
Size: 1082 4982
EcoRI G'AATT_C
Cuts at: 3373 3751 3373
Size: 378 5686
EcoRV GAT'ATC
Cuts at: 3274 3274
Size: 6064
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2676 3116 2676
Size: 440 5624
FspI TGC'GCA
Cuts at: 4570 4570
Size: 6064
HaeII r_GCGC'y
Cuts at: 1678 5211 1678
Size: 3533 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2760 2792 3039 3071 4705
Size: 32 135 32 1188 32 247 32 1634
Cuts at: 4705 4737 4779 4811 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1634 1188 247 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3408 3654 669
Size: 988 281 1470 246 3079
Fragments arranged by size:
3079 1470 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6064
HpaI GTT'AAC
Cuts at: 3654 3654
Size: 6064
KpnI G_GTAC'C
Cuts at: 3389 3389
Size: 6064
MfeI C'AATT_G
Cuts at: 3663 3663
Size: 6064
MluI A'CGCG_T
Cuts at: 5940 5940
Size: 6064
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3129 5178 5362 1305
Size: 1824 2049 184 2007
Fragments arranged by size:
2049 2007 1824 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 3306 10
Size: 54 1425 343 1474 2768
Fragments arranged by size:
2768 1474 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 4022 4381 4540 518
Size: 648 2856 359 159 2042
Fragments arranged by size:
2856 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2081 3041 3480 4157 5305 5550 1606
Size: 475 960 439 677 1148 245 2120
Fragments arranged by size:
2120 1148 960 677 475 439 245
NaeI GCC'GGC
Cuts at: 2026 2026
Size: 6064
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5512
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6064
NgoMIV G'CCGG_C
Cuts at: 2024 2024
Size: 6064
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6064
NotI GC'GGCC_GC
Cuts at: 3511 3511
Size: 6064
NsiI A_TGCA'T
Cuts at: 2860 2860
Size: 6064
NspI r_CATG'y
Cuts at: 1132 1229 2540 2879 2900 3226 3422 4967 1132
Size: 97 1311 339 21 326 196 1545 2229
Fragments arranged by size:
2229 1545 1311 339 326 196 97 21
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6064
PciI A'CATG_T
Cuts at: 2896 4963 2896
Size: 2067 3997
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6064
PfoI T'CCnGG_A
Cuts at: 3180 3180
Size: 6064
PmeI GTTT'AAAC
Cuts at: 3368 3368
Size: 6064
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2700 2732 4133 4165 5646 5678 2700
Size: 32 1401 32 1481 32 3086
Fragments arranged by size:
3086 1481 1401 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 2113 3195 2113
Size: 1082 4982
PshAI GACnn'nnGTC
Cuts at: 1613 2519 1613
Size: 906 5158
PsiI TTA'TAA
Cuts at: 3634 3634
Size: 6064
PstI C_TGCA'G
Cuts at: 830 2092 3416 830
Size: 1262 1324 3478
Fragments arranged by size:
3478 1324 1262
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6064
PvuII CAG'CTG
Cuts at: 2081 3041 2081
Size: 960 5104
SalI G'TCGA_C
Cuts at: 1936 3406 1936
Size: 1470 4594
SanDI GG'GwC_CC
Cuts at: 3195 3195
Size: 6064
SbfI CC_TGCA'GG
Cuts at: 2092 3416 2092
Size: 1324 4740
ScaI AGT'ACT
Cuts at: 4312 6024 4312
Size: 1712 4352
SfcI C'TryA_G
Cuts at: 826 1045 2088 2587 3412 4547 5228 5419 826
Size: 219 1043 499 825 1135 681 191 1471
Fragments arranged by size:
1471 1135 1043 825 681 499 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6064
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6064
SmaI CCC'GGG
Cuts at: 1872 3391 1872
Size: 1519 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2035 2117 3090 4168 5069 5331
Size: 197 926 92 82 973 1078 901 262
Cuts at: 5331 820
Size: 1553
Fragments arranged by size:
1553 1078 973 926 901 262 197 92
82
SnaBI TAC'GTA
Cuts at: 493 3855 493
Size: 3362 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6064
SphI G_CATG'C
Cuts at: 1132 2540 3422 1132
Size: 1408 882 3774
Fragments arranged by size:
3774 1408 882
SspI AAT'ATT
Cuts at: 5 52 3988 5
Size: 47 3936 2081
Fragments arranged by size:
3936 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6064
StyI C'CwwG_G
Cuts at: 513 1065 2559 2946 3503 513
Size: 552 1494 387 557 3074
Fragments arranged by size:
3074 1494 557 552 387
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2258 4091 4108 4261 4446 4891 325
Size: 1255 678 1833 17 153 185 445 1498
Fragments arranged by size:
1833 1498 1255 678 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4310 6022 96
Size: 274 80 33 51 1443 2333 1712 138
Fragments arranged by size:
2333 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2661 2693 6024 6056 2661
Size: 32 3331 32 2669
Fragments arranged by size:
3331 2669 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6064
XbaI T'CTAG_A
Cuts at: 3400 3400
Size: 6064
XhoI C'TCGA_G
Cuts at: 1943 2035 1943
Size: 92 5972
XmaI C'CCGG_G
Cuts at: 1870 3389 1870
Size: 1519 4545
XmnI GAAnn'nnTTC
Cuts at: 2639 2712 3100 4193 2639
Size: 73 388 1093 4510
Fragments arranged by size:
4510 1093 388 73
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3872 276
Size: 53 83 186 936 2338 2468
Fragments arranged by size:
2468 2338 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI
BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI
BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI
EarI EciI Eco57MI EcoO109I EcoRI EcoRV FalI FspI
HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI
MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV
NheI NotI NsiI NspI PasI PciI PflMI PfoI
PmeI PpiI PpuMI PshAI PsiI PstI PvuI PvuII
SalI SanDI SbfI ScaI SfcI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BplI BsiWI BsmBI BstXI BstZ17I DraIII EcoICRI EcoNI
FseI FspAI KasI NarI NruI PacI PmlI PspOMI
PspXI PsrI RsrII SacI SacII SapI SexAI SfiI
SfoI SrfI SwaI XcmI