Restriction map for
pFN21AB5467
(Circular) (Six-base) MAPSORT of: pFN21AB5467.seq Check: 8011 from: 1 to: 5689
pFN21AB5467 5689 bp
With 182 enzymes: SgfI *
April 15, 2010 09:31 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3499 278
Size: 53 83 186 936 1963 2468
Fragments arranged by size:
2468 1963 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3032 1937
Size: 1095 4594
Acc65I G'GTAC_C
Cuts at: 3010 3010
Size: 5689
AclI AA'CG_TT
Cuts at: 1563 2571 3816 4189 1563
Size: 1008 1245 373 3063
Fragments arranged by size:
3063 1245 1008 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2249 2780 3752 5136 5404 1632
Size: 617 531 972 1384 268 1917
Fragments arranged by size:
1917 1384 972 617 531 268
AfeI AGC'GCT
Cuts at: 2040 2521 2040
Size: 481 5208
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5492
AflIII A'CryG_T
Cuts at: 1203 2320 4588 5565 1203
Size: 1117 2268 977 1327
Fragments arranged by size:
2268 1327 1117 977
AgeI A'CCGG_T
Cuts at: 4520 4520
Size: 5689
AhdI GACnn_n'nnGTC
Cuts at: 2351 4418 2351
Size: 2067 3622
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5689
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5689
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2669 2701 2669
Size: 32 5657
AlwNI CAG_nnn'CTG
Cuts at: 2090 3432 5004 2090
Size: 1342 1572 2775
Fragments arranged by size:
2775 1572 1342
ApaI G_GGCC'C
Cuts at: 2779 2779
Size: 5689
ApaLI G'TGCA_C
Cuts at: 3746 4902 3746
Size: 1156 4533
ApoI r'AATT_y
Cuts at: 1493 2512 2998 3224 3376 5485 1493
Size: 1019 486 226 152 2109 1697
Fragments arranged by size:
2109 1697 1019 486 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4243 160
Size: 872 3211 1606
Fragments arranged by size:
3211 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5689
AvaI C'yCGr_G
Cuts at: 1870 1943 2034 2096 2927 3014 1870
Size: 73 91 62 831 87 4545
Fragments arranged by size:
4545 831 91 87 73 62
BamHI G'GATC_C
Cuts at: 2760 3019 3386 2760
Size: 259 367 5063
Fragments arranged by size:
5063 367 259
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2922 3010 4465 618
Size: 325 217 627 1135 88 1455 1842
Fragments arranged by size:
1842 1455 1135 627 325 217 88
BanII G_rGCy'C
Cuts at: 1391 2213 2422 2682 2723 2779 3008 1391
Size: 822 209 260 41 56 229 4072
Fragments arranged by size:
4072 822 260 229 209 56 41
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4718
BbvCI CC'TCA_GC
Cuts at: 2472 2855 2472
Size: 383 5306
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3880 3914 1903
Size: 34 1943 34 3678
Fragments arranged by size:
3678 1943 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3581 4791 3581
Size: 1210 4479
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5689
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2009 2541 2894
Size: 107 122 71 631 605 337 532 353
Cuts at: 2894 4300 136
Size: 1406 1525
Fragments arranged by size:
1525 1406 631 605 532 353 337 122
107 71
BglII A'GATC_T
Cuts at: 2841 5684 2841
Size: 2843 2846
BlpI GC'TnA_GC
Cuts at: 2270 3106 2270
Size: 836 4853
Bme1580I G_kGCm'C
Cuts at: 1790 2779 2899 3750 4906 1790
Size: 989 120 851 1156 2573
Fragments arranged by size:
2573 1156 989 851 120
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5689
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2069 3420 4378
Size: 416 53 83 399 24 652 1351 958
Cuts at: 4378 442
Size: 1753
Fragments arranged by size:
1753 1351 958 652 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5689
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2049 2306 2390 2049
Size: 257 84 5348
Fragments arranged by size:
5348 257 84
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2111 2298 2385 2696 4349 1239
Size: 872 187 87 311 1653 2579
Fragments arranged by size:
2579 1653 872 311 187 87
Bpu10I CC'TnA_GC
Cuts at: 2472 2669 2819 2855 2472
Size: 197 150 36 5306
Fragments arranged by size:
5306 197 150 36
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3814 4679 4977 3814
Size: 865 298 4526
Fragments arranged by size:
4526 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4352 882
Size: 617 2853 2219
Fragments arranged by size:
2853 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3480 493
Size: 711 2276 2702
Fragments arranged by size:
2702 2276 711
BsaBI GATnn'nnATC
Cuts at: 5683 5683
Size: 5689
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3496 3878 4513 275
Size: 53 83 186 936 1963 382 635 1451
Fragments arranged by size:
1963 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4122 4520 4794 4941 5352 4122
Size: 398 274 147 411 4459
Fragments arranged by size:
4459 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2173 2203 2669 2699 1178
Size: 30 165 30 770 30 466 30 4168
Fragments arranged by size:
4168 770 466 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2295 1186
Size: 1109 4580
BseYI C'CCAG_C
Cuts at: 4892 5388 4892
Size: 496 5193
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2040 2322 2498 1163
Size: 573 304 282 176 4354
Fragments arranged by size:
4354 573 304 282 176
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2317 3139 3900 4928 1540
Size: 275 186 316 822 761 1028 2301
Fragments arranged by size:
2301 1028 822 761 316 275 186
BsiHKAI G_wGCw'C
Cuts at: 2422 2682 3750 3835 4906 2422
Size: 260 1068 85 1071 3205
Fragments arranged by size:
3205 1071 1068 260 85
BsmI GAATG_Cn'
Cuts at: 2176 3200 3293 2176
Size: 1024 93 4572
Fragments arranged by size:
4572 1024 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2213 2422 2682 2723 2779 2899 3008
Size: 399 423 209 260 41 56 120 109
Cuts at: 3008 3750 3835 4906 1391
Size: 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 423 399 260 209 120
109 85 56 41
BspEI T'CCGG_A
Cuts at: 5352 5352
Size: 5689
BspHI T'CATG_A
Cuts at: 3578 5308 3578
Size: 1730 3959
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3044 844
Size: 2200 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3576 1394
Size: 429 1753 3507
Fragments arranged by size:
3507 1753 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4184 4358 65
Size: 1183 2936 174 1396
Fragments arranged by size:
2936 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2312 2432 2868 4333 4520 1700
Size: 110 502 120 436 1465 187 2869
Fragments arranged by size:
2869 1465 502 436 187 120 110
BsrGI T'GTAC_A
Cuts at: 96 2304 96
Size: 2208 3481
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5689
BssSI C'ACGA_G
Cuts at: 3749 4761 3749
Size: 1012 4677
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5689
BstBI TT'CG_AA
Cuts at: 4493 4493
Size: 5689
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5689
BstXI CCAn_nnnn'nTGG
Cuts at: 2545 2545
Size: 5689
BstYI r'GATC_y
Cuts at: 1972 2760 2841 3019 3386 3771 3788 5240 5326
Size: 788 81 178 367 385 17 1452 86
Cuts at: 5326 5338 5684 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 788 385 367 346 178 86
81 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2214 2530 2980 513
Size: 552 939 210 316 450 3222
Fragments arranged by size:
3222 939 552 450 316 210
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4950
Fragments arranged by size:
4950 683 56
BtsI GCAGTG_nn'
Cuts at: 3205 3999 4019 3205
Size: 794 20 4875
Fragments arranged by size:
4875 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5689
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5654
DraI TTT'AAA
Cuts at: 2993 2993
Size: 5689
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4696 809
Size: 507 3380 1802
Fragments arranged by size:
3380 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2314 2547 3136
Size: 54 1425 50 275 18 484 233 589
Cuts at: 3136 4025 5654 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 889 589 484 275 233 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2314 3136 1537
Size: 275 502 822 4090
Fragments arranged by size:
4090 822 502 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2225 3619 1353
Size: 872 1394 3423
Fragments arranged by size:
3423 1394 872
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4276 4650 4796 387
Size: 1064 138 2687 374 146 1280
Fragments arranged by size:
2687 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2420 2680 2420
Size: 260 5429
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2111 2249 2298 2385 2696 2780 3752
Size: 393 479 138 49 87 311 84 972
Cuts at: 3752 4349 5136 5404 1239
Size: 597 787 268 1524
Fragments arranged by size:
1524 972 787 597 479 393 311 268
138 87 84 49
EcoO109I rG'GnC_Cy
Cuts at: 2147 2161 2369 2776 2914 2945 2147
Size: 14 208 407 138 31 4891
Fragments arranged by size:
4891 407 208 138 31 14
EcoRI G'AATT_C
Cuts at: 2998 3376 2998
Size: 378 5311
FspI TGC'GCA
Cuts at: 4195 4195
Size: 5689
HaeII r_GCGC'y
Cuts at: 1678 2031 2042 2523 2938 4836 1678
Size: 353 11 481 415 1898 2531
Fragments arranged by size:
2531 1898 481 415 353 11
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2337 2369 2812 2844 4330
Size: 32 135 32 765 32 443 32 1486
Cuts at: 4330 4362 4404 4436 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1486 765 443 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2569 3033 3279 669
Size: 988 281 631 464 246 3079
Fragments arranged by size:
3079 988 631 464 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5689
HpaI GTT'AAC
Cuts at: 3279 3279
Size: 5689
KpnI G_GTAC'C
Cuts at: 3014 3014
Size: 5689
MfeI C'AATT_G
Cuts at: 3288 3288
Size: 5689
MluI A'CGCG_T
Cuts at: 5565 5565
Size: 5689
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4803 4987 1305
Size: 3498 184 2007
Fragments arranged by size:
3498 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2549 10
Size: 54 1425 343 717 3150
Fragments arranged by size:
3150 1425 717 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2967 3647 4006 4165 518
Size: 648 1801 680 359 159 2042
Fragments arranged by size:
2042 1801 680 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2532 2859 3105 3782 4930 5175 1606
Size: 926 327 246 677 1148 245 2120
Fragments arranged by size:
2120 1148 926 677 327 246 245
NaeI GCC'GGC
Cuts at: 2434 2870 2434
Size: 436 5253
NcoI C'CATG_G
Cuts at: 513 1065 2004 2980 513
Size: 552 939 976 3222
Fragments arranged by size:
3222 976 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5689
NgoMIV G'CCGG_C
Cuts at: 2432 2868 2432
Size: 436 5253
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5689
NotI GC'GGCC_GC
Cuts at: 3136 3136
Size: 5689
NruI TCG'CGA
Cuts at: 2424 2424
Size: 5689
NspI r_CATG'y
Cuts at: 1132 1229 2324 2966 3047 4592 1132
Size: 97 1095 642 81 1545 2229
Fragments arranged by size:
2229 1545 1095 642 97 81
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5689
PciI A'CATG_T
Cuts at: 2320 4588 2320
Size: 2268 3421
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5689
PmeI GTTT'AAAC
Cuts at: 2993 2993
Size: 5689
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2669 2701 3758 3790 5271 5303 2669
Size: 32 1057 32 1481 32 3055
Fragments arranged by size:
3055 1481 1057 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 2369 2914 2369
Size: 545 5144
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5689
PsiI TTA'TAA
Cuts at: 3259 3259
Size: 5689
PspOMI G'GGCC_C
Cuts at: 2775 2775
Size: 5689
PspXI vC'TCGA_Gb
Cuts at: 2034 2034
Size: 5689
PstI C_TGCA'G
Cuts at: 830 2017 2880 3041 830
Size: 1187 863 161 3478
Fragments arranged by size:
3478 1187 863 161
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5689
SacI G_AGCT'C
Cuts at: 2422 2682 2422
Size: 260 5429
SacII CC_GC'GG
Cuts at: 2533 2533
Size: 5689
SalI G'TCGA_C
Cuts at: 1936 3031 1936
Size: 1095 4594
SanDI GG'GwC_CC
Cuts at: 2914 2914
Size: 5689
SbfI CC_TGCA'GG
Cuts at: 3041 3041
Size: 5689
ScaI AGT'ACT
Cuts at: 3937 5649 3937
Size: 1712 3977
SfcI C'TryA_G
Cuts at: 826 1045 2013 2876 3037 4172 4853 5044 826
Size: 219 968 863 161 1135 681 191 1471
Fragments arranged by size:
1471 1135 968 863 681 219 191 161
SfiI GGCCn_nnn'nGGCC
Cuts at: 2541 2541
Size: 5689
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5689
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5689
SmaI CCC'GGG
Cuts at: 1872 3016 1872
Size: 1144 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2034 3793 4694 4956 820
Size: 197 926 91 1759 901 262 1553
Fragments arranged by size:
1759 1553 926 901 262 197 91
SnaBI TAC'GTA
Cuts at: 493 3480 493
Size: 2987 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5689
SphI G_CATG'C
Cuts at: 1132 3047 1132
Size: 1915 3774
SspI AAT'ATT
Cuts at: 5 52 3613 5
Size: 47 3561 2081
Fragments arranged by size:
3561 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5689
StyI C'CwwG_G
Cuts at: 513 1065 2004 2883 2980 3128 513
Size: 552 939 879 97 148 3074
Fragments arranged by size:
3074 939 879 552 148 97
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3716 3733 3886 4071 4516 325
Size: 1255 2136 17 153 185 445 1498
Fragments arranged by size:
2136 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2304 3935 5647
Size: 274 80 33 51 1443 327 1631 1712
Cuts at: 5647 96
Size: 138
Fragments arranged by size:
1712 1631 1443 327 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2124 2156 2173 2205 5649 5681 2124
Size: 32 17 32 3444 32 2132
Fragments arranged by size:
3444 2132 32 32 32 17
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5689
XbaI T'CTAG_A
Cuts at: 3025 3025
Size: 5689
XhoI C'TCGA_G
Cuts at: 1943 2034 1943
Size: 91 5598
XmaI C'CCGG_G
Cuts at: 1870 3014 1870
Size: 1144 4545
XmnI GAAnn'nnTTC
Cuts at: 3818 3818
Size: 5689
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3497 276
Size: 53 83 186 936 1963 2468
Fragments arranged by size:
2468 1963 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI
BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII
BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I
EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI
MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI
NdeI NgoMIV NheI NotI NruI NspI PasI PciI
PflMI PmeI PpiI PpuMI PshAI PsiI PspOMI PspXI
PstI PvuI SacI SacII SalI SanDI SbfI ScaI
SfcI SfiI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AscI AvrII BaeI BbeI BfrBI BsiWI BsmBI
BstZ17I Bsu36I DraIII EcoNI EcoRV FalI FseI FspAI
KasI NarI NsiI PacI PfoI PmlI PsrI PvuII
RsrII SapI SexAI SfoI SrfI SwaI XcmI