Restriction map for
pFN21AB5462
(Circular) (Six-base) MAPSORT of: pFN21AB5462.seq Check: 8309 from: 1 to: 5833
pFN21AB5462 5833 bp
With 182 enzymes: SgfI *
April 15, 2010 09:31 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 2574 3643 278
Size: 53 83 186 936 1038 1069 2468
Fragments arranged by size:
2468 1069 1038 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2283 3176 1937
Size: 346 893 4594
Fragments arranged by size:
4594 893 346
Acc65I G'GTAC_C
Cuts at: 3154 3154
Size: 5833
AclI AA'CG_TT
Cuts at: 1563 3960 4333 1563
Size: 2397 373 3063
Fragments arranged by size:
3063 2397 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3896 5280 5548 1632
Size: 2264 1384 268 1917
Fragments arranged by size:
2264 1917 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5636
AflIII A'CryG_T
Cuts at: 1203 2727 4732 5709 1203
Size: 1524 2005 977 1327
Fragments arranged by size:
2005 1524 1327 977
AgeI A'CCGG_T
Cuts at: 4664 4664
Size: 5833
AhdI GACnn_n'nnGTC
Cuts at: 4562 4562
Size: 5833
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5833
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5833
AlwNI CAG_nnn'CTG
Cuts at: 3576 5148 3576
Size: 1572 4261
ApaI G_GGCC'C
Cuts at: 2779 2779
Size: 5833
ApaLI G'TGCA_C
Cuts at: 3890 5046 3890
Size: 1156 4677
ApoI r'AATT_y
Cuts at: 1493 3142 3368 3520 5629 1493
Size: 1649 226 152 2109 1697
Fragments arranged by size:
2109 1697 1649 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4387 160
Size: 872 3355 1606
Fragments arranged by size:
3355 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5833
AvaI C'yCGr_G
Cuts at: 1870 1943 2353 2780 3158 1870
Size: 73 410 427 378 4545
Fragments arranged by size:
4545 427 410 378 73
BamHI G'GATC_C
Cuts at: 3163 3530 3163
Size: 367 5466
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3154 4609 618
Size: 325 217 627 1367 1455 1842
Fragments arranged by size:
1842 1455 1367 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2158 2779 2829 3152 1391
Size: 767 621 50 323 4072
Fragments arranged by size:
4072 767 621 323 50
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4862
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2119 2153 3112 3146 4024 4058 1903
Size: 34 182 34 959 34 878 34 3678
Fragments arranged by size:
3678 959 878 182 34 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3725 4935 3725
Size: 1210 4623
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5833
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4444 136
Size: 107 122 71 631 605 2772 1525
Fragments arranged by size:
2772 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2991 5828 2991
Size: 2837 2996
BlpI GC'TnA_GC
Cuts at: 3250 3250
Size: 5833
Bme1580I G_kGCm'C
Cuts at: 1790 2511 2779 3894 5050 1790
Size: 721 268 1115 1156 2573
Fragments arranged by size:
2573 1156 1115 721 268
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5833
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3564 4522 442
Size: 416 53 83 399 24 2147 958 1753
Fragments arranged by size:
2147 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5833
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3121 3121
Size: 5833
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2172 2180 2726 4493 1239
Size: 933 8 546 1767 2579
Fragments arranged by size:
2579 1767 933 546 8
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3958 4823 5121 3958
Size: 865 298 4670
Fragments arranged by size:
4670 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2079 2387 2931 4496 882
Size: 617 580 308 544 1565 2219
Fragments arranged by size:
2219 1565 617 580 544 308
BsaAI yAC'GTr
Cuts at: 493 1204 3624 493
Size: 711 2420 2702
Fragments arranged by size:
2702 2420 711
BsaBI GATnn'nnATC
Cuts at: 5827 5827
Size: 5833
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2571 3640 4022 4657
Size: 53 83 186 936 1038 1069 382 635
Cuts at: 4657 275
Size: 1451
Fragments arranged by size:
1451 1069 1038 936 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 2646 4266 4664 4938 5085 5496 2646
Size: 1620 398 274 147 411 2983
Fragments arranged by size:
2983 1620 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2270 2300 1178
Size: 30 165 30 867 30 4711
Fragments arranged by size:
4711 867 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2207 2321 2330 1186
Size: 1021 114 9 4689
Fragments arranged by size:
4689 1021 114 9
BseYI C'CCAG_C
Cuts at: 5036 5532 5036
Size: 496 5337
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2741 2748 1163
Size: 573 1005 7 4248
Fragments arranged by size:
4248 1005 573 7
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2129 2566 3283 4044 5072 1540
Size: 275 186 128 437 717 761 1028 2301
Fragments arranged by size:
2301 1028 761 717 437 275 186 128
BsiHKAI G_wGCw'C
Cuts at: 2199 2235 2331 2439 2904 3894 3979 5050 2199
Size: 36 96 108 465 990 85 1071 2982
Fragments arranged by size:
2982 1071 990 465 108 96 85 36
BsmI GAATG_Cn'
Cuts at: 3344 3437 3344
Size: 93 5740
BsmBI CGTCTCn'nnnn_
Cuts at: 2578 2702 2578
Size: 124 5709
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2158 2199 2235 2331 2439 2511 2779
Size: 399 368 41 36 96 108 72 268
Cuts at: 2779 2829 2904 3152 3894 3979 5050 1391
Size: 50 75 248 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 399 368 268 248 108
96 85 75 72 50 41 36
BspEI T'CCGG_A
Cuts at: 2646 5496 2646
Size: 2850 2983
BspHI T'CATG_A
Cuts at: 3722 5452 3722
Size: 1730 4103
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2880 3188 844
Size: 2036 308 3489
Fragments arranged by size:
3489 2036 308
BsrBI CCG'CTC
Cuts at: 1394 1823 2347 3720 1394
Size: 429 524 1373 3507
Fragments arranged by size:
3507 1373 524 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2556 4328 4502 65
Size: 1183 1308 1772 174 1396
Fragments arranged by size:
1772 1396 1308 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2407 2561 4477 4664 1700
Size: 110 597 154 1916 187 2869
Fragments arranged by size:
2869 1916 597 187 154 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5833
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5833
BssSI C'ACGA_G
Cuts at: 3893 4905 3893
Size: 1012 4821
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2326 2434 794
Size: 1532 108 4193
Fragments arranged by size:
4193 1532 108
BstBI TT'CG_AA
Cuts at: 4637 4637
Size: 5833
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5833
BstYI r'GATC_y
Cuts at: 1972 2991 3163 3530 3915 3932 5384 5470 5482
Size: 1019 172 367 385 17 1452 86 12
Cuts at: 5482 5828 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 1019 385 367 346 172 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2226 513
Size: 552 939 222 4120
Fragments arranged by size:
4120 939 552 222
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2008 3104 487
Size: 683 56 782 1096 3216
Fragments arranged by size:
3216 1096 782 683 56
BtsI GCAGTG_nn'
Cuts at: 3349 4143 4163 3349
Size: 794 20 5019
Fragments arranged by size:
5019 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5833
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 3010 3045 529
Size: 35 2446 35 3317
Fragments arranged by size:
3317 2446 35 35
DraI TTT'AAA
Cuts at: 3137 3137
Size: 5833
DraIII CAC_nnn'GTG
Cuts at: 2239 2239
Size: 5833
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4840 809
Size: 507 3524 1802
Fragments arranged by size:
3524 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2126 2563 3280
Size: 54 1425 50 275 18 296 437 717
Cuts at: 3280 4169 5798 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 889 717 437 296 275 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2126 2563 3280 1537
Size: 275 314 437 717 4090
Fragments arranged by size:
4090 717 437 314 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2177 3090 3763 1353
Size: 824 913 673 3423
Fragments arranged by size:
3423 913 824 673
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4420 4794 4940 387
Size: 1064 138 2831 374 146 1280
Fragments arranged by size:
2831 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2172 2180 2726 3896 4493 5280 5548
Size: 393 540 8 546 1170 597 787 268
Cuts at: 5548 1239
Size: 1524
Fragments arranged by size:
1524 1170 787 597 546 540 393 268
8
EcoNI CCTnn'n_nnAGG
Cuts at: 2336 2336
Size: 5833
EcoO109I rG'GnC_Cy
Cuts at: 2167 2341 2759 2776 2167
Size: 174 418 17 5224
Fragments arranged by size:
5224 418 174 17
EcoRI G'AATT_C
Cuts at: 3142 3520 3142
Size: 378 5455
EcoRV GAT'ATC
Cuts at: 2980 2980
Size: 5833
FspI TGC'GCA
Cuts at: 2600 4339 2600
Size: 1739 4094
HaeII r_GCGC'y
Cuts at: 1678 4980 1678
Size: 3302 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 4474 4506 4548 4580 1373
Size: 32 135 32 2902 32 42 32 2626
Fragments arranged by size:
2902 2626 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2614 3177 3423 669
Size: 988 281 676 563 246 3079
Fragments arranged by size:
3079 988 676 563 281 246
HindIII A'AGCT_T
Cuts at: 748 2963 748
Size: 2215 3618
HpaI GTT'AAC
Cuts at: 3423 3423
Size: 5833
KpnI G_GTAC'C
Cuts at: 3158 3158
Size: 5833
MfeI C'AATT_G
Cuts at: 3432 3432
Size: 5833
MluI A'CGCG_T
Cuts at: 5709 5709
Size: 5833
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2611 2886 4947 5131 1305
Size: 1306 275 2061 184 2007
Fragments arranged by size:
2061 2007 1306 275 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4011
Fragments arranged by size:
4011 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2141 2423 2549 3791 4150 4309 518
Size: 648 975 282 126 1242 359 159 2042
Fragments arranged by size:
2042 1242 975 648 359 282 159 126
MspA1I CmG'CkG
Cuts at: 1606 2299 3249 3926 5074 5319 1606
Size: 693 950 677 1148 245 2120
Fragments arranged by size:
2120 1148 950 693 677 245
NaeI GCC'GGC
Cuts at: 2409 2563 2409
Size: 154 5679
NcoI C'CATG_G
Cuts at: 513 1065 2004 2226 513
Size: 552 939 222 4120
Fragments arranged by size:
4120 939 552 222
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5833
NgoMIV G'CCGG_C
Cuts at: 2407 2561 2407
Size: 154 5679
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5833
NotI GC'GGCC_GC
Cuts at: 3280 3280
Size: 5833
NspI r_CATG'y
Cuts at: 1132 1229 2140 3191 4736 1132
Size: 97 911 1051 1545 2229
Fragments arranged by size:
2229 1545 1051 911 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5833
PciI A'CATG_T
Cuts at: 4732 4732
Size: 5833
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2038 2836 1108
Size: 930 798 4105
Fragments arranged by size:
4105 930 798
PmeI GTTT'AAAC
Cuts at: 3137 3137
Size: 5833
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2774 2806 3902 3934 5415 5447 2774
Size: 32 1096 32 1481 32 3160
Fragments arranged by size:
3160 1481 1096 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 2167 2167
Size: 5833
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5833
PsiI TTA'TAA
Cuts at: 3403 3403
Size: 5833
PspOMI G'GGCC_C
Cuts at: 2775 2775
Size: 5833
PstI C_TGCA'G
Cuts at: 830 2304 3185 830
Size: 1474 881 3478
Fragments arranged by size:
3478 1474 881
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5833
PvuII CAG'CTG
Cuts at: 2299 2299
Size: 5833
SalI G'TCGA_C
Cuts at: 1936 3175 1936
Size: 1239 4594
SbfI CC_TGCA'GG
Cuts at: 3185 3185
Size: 5833
ScaI AGT'ACT
Cuts at: 4081 5793 4081
Size: 1712 4121
SfcI C'TryA_G
Cuts at: 826 1045 2300 3181 4316 4997 5188 826
Size: 219 1255 881 1135 681 191 1471
Fragments arranged by size:
1471 1255 1135 881 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5833
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5833
SmaI CCC'GGG
Cuts at: 1872 3160 1872
Size: 1288 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3937 4838 5100 820
Size: 197 926 1994 901 262 1553
Fragments arranged by size:
1994 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3624 493
Size: 3131 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5833
SphI G_CATG'C
Cuts at: 1132 3191 1132
Size: 2059 3774
SspI AAT'ATT
Cuts at: 5 52 3757 5
Size: 47 3705 2081
Fragments arranged by size:
3705 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5833
StyI C'CwwG_G
Cuts at: 513 1065 2004 2226 2289 3272 513
Size: 552 939 222 63 983 3074
Fragments arranged by size:
3074 983 939 552 222 63
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2955 3064 3860 3877 4030 4215 4660
Size: 1255 1375 109 796 17 153 185 445
Cuts at: 4660 325
Size: 1498
Fragments arranged by size:
1498 1375 1255 796 445 185 153 109
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2724 3015 4079
Size: 274 80 33 51 1443 747 291 1064
Cuts at: 4079 5791 96
Size: 1712 138
Fragments arranged by size:
1712 1443 1064 747 291 274 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5793 5825 5793
Size: 32 5801
Tth111I GACn'n_nGTC
Cuts at: 1316 2492 1316
Size: 1176 4657
XbaI T'CTAG_A
Cuts at: 2476 3169 2476
Size: 693 5140
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2035 2548 2035
Size: 513 5320
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5833
XmaI C'CCGG_G
Cuts at: 1870 3158 1870
Size: 1288 4545
XmnI GAAnn'nnTTC
Cuts at: 3962 3962
Size: 5833
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 2572 3641 276
Size: 53 83 186 936 1038 1069 2468
Fragments arranged by size:
2468 1069 1038 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI
BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI
EarI EciI Eco57MI EcoNI EcoO109I EcoRI EcoRV FspI
HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI
MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV
NheI NotI NspI PasI PciI PflMI PmeI PpiI
PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SalI
SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI AscI AvrII BaeI BbeI BbvCI
BfrBI Bpu10I BsiWI BstXI BstZ17I Bsu36I EcoICRI FalI
FseI FspAI KasI NarI NruI NsiI PacI PfoI
PmlI PspXI PsrI RsrII SacI SacII SanDI SapI
SexAI SfiI SfoI SrfI SwaI