Restriction map for
pFN21AB5459
(Circular) (Six-base) MAPSORT of: pFN21AB5459.seq Check: 9083 from: 1 to: 5878
pFN21AB5459 5878 bp
With 182 enzymes: SgfI *
October 20, 2009 10:35 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3688 278
Size: 53 83 186 936 2152 2468
Fragments arranged by size:
2468 2152 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2367 3221 1937
Size: 430 854 4594
Fragments arranged by size:
4594 854 430
Acc65I G'GTAC_C
Cuts at: 3199 3199
Size: 5878
AclI AA'CG_TT
Cuts at: 1563 4005 4378 1563
Size: 2442 373 3063
Fragments arranged by size:
3063 2442 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3042 3941 5325 5593 1632
Size: 1410 899 1384 268 1917
Fragments arranged by size:
1917 1410 1384 899 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5681
AflIII A'CryG_T
Cuts at: 1203 2615 4777 5754 1203
Size: 1412 2162 977 1327
Fragments arranged by size:
2162 1412 1327 977
AgeI A'CCGG_T
Cuts at: 4709 4709
Size: 5878
AhdI GACnn_n'nnGTC
Cuts at: 4607 4607
Size: 5878
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5878
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5878
AlwNI CAG_nnn'CTG
Cuts at: 3621 5193 3621
Size: 1572 4306
ApaLI G'TGCA_C
Cuts at: 3935 5091 3935
Size: 1156 4722
ApoI r'AATT_y
Cuts at: 1493 3187 3413 3565 5674 1493
Size: 1694 226 152 2109 1697
Fragments arranged by size:
2109 1697 1694 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4432 160
Size: 872 3400 1606
Fragments arranged by size:
3400 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5878
AvaI C'yCGr_G
Cuts at: 1870 1943 2398 3203 1870
Size: 73 455 805 4545
Fragments arranged by size:
4545 805 455 73
BamHI G'GATC_C
Cuts at: 2139 2180 2855 3148 3208 3575 2139
Size: 41 675 293 60 367 4442
Fragments arranged by size:
4442 675 367 293 60 41
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2476 3199 4654 618
Size: 325 217 627 689 723 1455 1842
Fragments arranged by size:
1842 1455 723 689 627 325 217
BanII G_rGCy'C
Cuts at: 1391 3044 3197 1391
Size: 1653 153 4072
Fragments arranged by size:
4072 1653 153
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2229 2247 928
Size: 971 330 18 4559
Fragments arranged by size:
4559 971 330 18
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4069 4103 1903
Size: 34 2132 34 3678
Fragments arranged by size:
3678 2132 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3770 4980 3770
Size: 1210 4668
BclI T'GATC_A
Cuts at: 1548 2271 1548
Size: 723 5155
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2651 4489 136
Size: 107 122 71 631 605 979 1838 1525
Fragments arranged by size:
1838 1525 979 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2965 5873 2965
Size: 2908 2970
BlpI GC'TnA_GC
Cuts at: 3295 3295
Size: 5878
Bme1580I G_kGCm'C
Cuts at: 1790 3939 5095 1790
Size: 2149 1156 2573
Fragments arranged by size:
2573 2149 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5878
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2383 3609 4567
Size: 416 53 83 399 24 966 1226 958
Cuts at: 4567 442
Size: 1753
Fragments arranged by size:
1753 1226 966 958 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5878
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 4538 1239
Size: 3299 2579
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2397 3063 4003 4868 5166 2397
Size: 666 940 865 298 3109
Fragments arranged by size:
3109 940 865 666 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2272 4541 882
Size: 617 773 2269 2219
Fragments arranged by size:
2269 2219 773 617
BsaAI yAC'GTr
Cuts at: 493 1204 3669 493
Size: 711 2465 2702
Fragments arranged by size:
2702 2465 711
BsaBI GATnn'nnATC
Cuts at: 2270 2591 5872 2270
Size: 321 3281 2276
Fragments arranged by size:
3281 2276 321
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3685 4067 4702 275
Size: 53 83 186 936 2152 382 635 1451
Fragments arranged by size:
2152 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4311 4709 4983 5130 5541 4311
Size: 398 274 147 411 4648
Fragments arranged by size:
4648 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5653
Fragments arranged by size:
5653 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2985 3042 1186
Size: 1799 57 4022
Fragments arranged by size:
4022 1799 57
BseYI C'CCAG_C
Cuts at: 2184 5081 5577 2184
Size: 2897 496 2485
Fragments arranged by size:
2897 2485 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 3194 1163
Size: 573 1458 3847
Fragments arranged by size:
3847 1458 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3328 4089 5117 1540
Size: 275 186 1327 761 1028 2301
Fragments arranged by size:
2301 1327 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3939 4024 5095 3939
Size: 85 1071 4722
Fragments arranged by size:
4722 1071 85
BsmI GAATG_Cn'
Cuts at: 3389 3482 3389
Size: 93 5785
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 3044 3197 3939 4024 5095 1391
Size: 399 1254 153 742 85 1071 2174
Fragments arranged by size:
2174 1254 1071 742 399 153 85
BspEI T'CCGG_A
Cuts at: 5541 5541
Size: 5878
BspHI T'CATG_A
Cuts at: 2274 3767 5497 2274
Size: 1493 1730 2655
Fragments arranged by size:
2655 1730 1493
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2220 2886 3166 3233 844
Size: 1376 666 280 67 3489
Fragments arranged by size:
3489 1376 666 280 67
BsrBI CCG'CTC
Cuts at: 1394 1823 3765 1394
Size: 429 1942 3507
Fragments arranged by size:
3507 1942 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4373 4547 65
Size: 1183 3125 174 1396
Fragments arranged by size:
3125 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4522 4709 1700
Size: 110 2712 187 2869
Fragments arranged by size:
2869 2712 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5878
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5878
BssSI C'ACGA_G
Cuts at: 2121 3938 4950 2121
Size: 1817 1012 3049
Fragments arranged by size:
3049 1817 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5878
BstBI TT'CG_AA
Cuts at: 4682 4682
Size: 5878
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5878
BstYI r'GATC_y
Cuts at: 1972 2139 2180 2443 2855 2965 3148 3208 3575
Size: 167 41 263 412 110 183 60 367
Cuts at: 3575 3960 3977 5429 5515 5527 5873 1972
Size: 385 17 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 412 385 367 346 263 183
167 110 86 60 41 17 12
Bsu36I CC'TnA_GG
Cuts at: 2322 2322
Size: 5878
BtgI C'CryG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4387
Fragments arranged by size:
4387 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5139
Fragments arranged by size:
5139 683 56
BtsI GCAGTG_nn'
Cuts at: 2077 2195 3394 4188 4208 2077
Size: 118 1199 794 20 3747
Fragments arranged by size:
3747 1199 794 118 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5878
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5843
DraI TTT'AAA
Cuts at: 3182 3182
Size: 5878
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4885 809
Size: 507 3569 1802
Fragments arranged by size:
3569 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2089 2677 2931
Size: 54 1425 50 275 18 259 588 254
Cuts at: 2931 3325 4214 5843 8
Size: 394 889 1629 43
Fragments arranged by size:
1629 1425 889 588 394 275 259 254
54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3325 1537
Size: 275 1513 4090
Fragments arranged by size:
4090 1513 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3808 1353
Size: 2455 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4465 4839 4985 387
Size: 1064 138 2876 374 146 1280
Fragments arranged by size:
2876 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 3042 3941 4538 5325 5593 1239
Size: 393 1410 899 597 787 268 1524
Fragments arranged by size:
1524 1410 899 787 597 393 268
EcoO109I rG'GnC_Cy
Cuts at: 2327 2327
Size: 5878
EcoRI G'AATT_C
Cuts at: 3187 3565 3187
Size: 378 5500
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2246 2326 2446 2246
Size: 80 120 5678
Fragments arranged by size:
5678 120 80
FspI TGC'GCA
Cuts at: 2746 4384 2746
Size: 1638 4240
FspAI rTGC'GCAy
Cuts at: 2746 2746
Size: 5878
HaeII r_GCGC'y
Cuts at: 1678 5025 1678
Size: 3347 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 4519 4551 4593 4625 1373
Size: 32 135 32 2947 32 42 32 2626
Fragments arranged by size:
2947 2626 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3222 3468 669
Size: 988 281 1284 246 3079
Fragments arranged by size:
3079 1284 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5878
HpaI GTT'AAC
Cuts at: 3468 3468
Size: 5878
KpnI G_GTAC'C
Cuts at: 3203 3203
Size: 5878
MfeI C'AATT_G
Cuts at: 3477 3477
Size: 5878
MluI A'CGCG_T
Cuts at: 5754 5754
Size: 5878
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4992 5176 1305
Size: 3687 184 2007
Fragments arranged by size:
3687 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2679 2933 10
Size: 54 1425 343 847 254 2955
Fragments arranged by size:
2955 1425 847 343 254 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2009 3836 4195 4354 518
Size: 648 843 1827 359 159 2042
Fragments arranged by size:
2042 1827 843 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 3294 3971 5119 5364 1606
Size: 1688 677 1148 245 2120
Fragments arranged by size:
2120 1688 1148 677 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4387
Fragments arranged by size:
4387 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5878
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5878
NotI GC'GGCC_GC
Cuts at: 3325 3325
Size: 5878
NspI r_CATG'y
Cuts at: 1132 1229 2518 2619 2745 3236 4781 1132
Size: 97 1289 101 126 491 1545 2229
Fragments arranged by size:
2229 1545 1289 491 126 101 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5878
PciI A'CATG_T
Cuts at: 2615 4777 2615
Size: 2162 3716
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5878
PfoI T'CCnGG_A
Cuts at: 2851 2851
Size: 5878
PmeI GTTT'AAAC
Cuts at: 3182 3182
Size: 5878
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2288 2320 3101 3133 3947 3979 5460 5492 2288
Size: 32 781 32 814 32 1481 32 2674
Fragments arranged by size:
2674 1481 814 781 32 32 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5878
PsiI TTA'TAA
Cuts at: 3448 3448
Size: 5878
PstI C_TGCA'G
Cuts at: 830 3230 830
Size: 2400 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5878
SalI G'TCGA_C
Cuts at: 1936 3220 1936
Size: 1284 4594
SbfI CC_TGCA'GG
Cuts at: 3230 3230
Size: 5878
ScaI AGT'ACT
Cuts at: 4126 5838 4126
Size: 1712 4166
SfcI C'TryA_G
Cuts at: 826 1045 3226 4361 5042 5233 826
Size: 219 2181 1135 681 191 1471
Fragments arranged by size:
2181 1471 1135 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5878
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5878
SmaI CCC'GGG
Cuts at: 1872 3205 1872
Size: 1333 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2412 3078 3982 4883 5145 820
Size: 197 926 469 666 904 901 262 1553
Fragments arranged by size:
1553 926 904 901 666 469 262 197
SnaBI TAC'GTA
Cuts at: 493 3669 493
Size: 3176 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5878
SphI G_CATG'C
Cuts at: 1132 3236 1132
Size: 2104 3774
SspI AAT'ATT
Cuts at: 5 52 2825 3802 5
Size: 47 2773 977 2081
Fragments arranged by size:
2773 2081 977 47
StuI AGG'CCT
Cuts at: 1517 2788 1517
Size: 1271 4607
StyI C'CwwG_G
Cuts at: 513 1065 2004 2685 3317 513
Size: 552 939 681 632 3074
Fragments arranged by size:
3074 939 681 632 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2349 2365 3905 3922 4075 4260 4705
Size: 1255 769 16 1540 17 153 185 445
Cuts at: 4705 325
Size: 1498
Fragments arranged by size:
1540 1498 1255 769 445 185 153 17
16
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2298 4124 5836
Size: 274 80 33 51 1443 321 1826 1712
Cuts at: 5836 96
Size: 138
Fragments arranged by size:
1826 1712 1443 321 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5838 5870 5838
Size: 32 5846
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5878
XbaI T'CTAG_A
Cuts at: 3214 3214
Size: 5878
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5878
XmaI C'CCGG_G
Cuts at: 1870 3203 1870
Size: 1333 4545
XmnI GAAnn'nnTTC
Cuts at: 4007 4007
Size: 5878
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3686 276
Size: 53 83 186 936 2152 2468
Fragments arranged by size:
2468 2152 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BcgI BciVI BclI
BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI
BstBI BstEII BstYI Bsu36I BtgI BtgZI BtsI ClaI
CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI
EcoO109I EcoRI FalI FspI FspAI HaeII Hin4I HincII
HindIII HpaI KpnI MfeI MluI MmeI MscI MslI
MspA1I NcoI NdeI NheI NotI NspI PasI PciI
PflMI PfoI PmeI PpiI PshAI PsiI PstI PvuI
SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstXI BstZ17I
DraIII EcoICRI EcoNI EcoRV FseI KasI NaeI NarI
NgoMIV NruI NsiI PacI PmlI PpuMI PspOMI PspXI
PsrI PvuII RsrII SacI SacII SanDI SapI SexAI
SfiI SfoI SrfI SwaI XcmI