Restriction map for
pFN21AB5454
(Circular) (Six-base) MAPSORT of: pFN21AB5454.seq Check: 8727 from: 1 to: 6247
pFN21AB5454 6247 bp
With 182 enzymes: SgfI *
April 15, 2010 09:31 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3504 4057 278
Size: 53 83 186 936 1968 553 2468
Fragments arranged by size:
2468 1968 936 553 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2897 3590 1937
Size: 960 693 4594
Fragments arranged by size:
4594 960 693
Acc65I G'GTAC_C
Cuts at: 3568 3568
Size: 6247
AclI AA'CG_TT
Cuts at: 1563 4374 4747 1563
Size: 2811 373 3063
Fragments arranged by size:
3063 2811 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2192 2369 4310 5694 5962 1632
Size: 560 177 1941 1384 268 1917
Fragments arranged by size:
1941 1917 1384 560 268 177
AfeI AGC'GCT
Cuts at: 2081 2081
Size: 6247
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6050
AflIII A'CryG_T
Cuts at: 1203 5146 6123 1203
Size: 3943 977 1327
Fragments arranged by size:
3943 1327 977
AgeI A'CCGG_T
Cuts at: 5078 5078
Size: 6247
AhdI GACnn_n'nnGTC
Cuts at: 3010 4976 3010
Size: 1966 4281
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6247
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2847 3374 1184
Size: 1663 527 4057
Fragments arranged by size:
4057 1663 527
AlwNI CAG_nnn'CTG
Cuts at: 3067 3990 5562 3067
Size: 923 1572 3752
Fragments arranged by size:
3752 1572 923
ApaLI G'TGCA_C
Cuts at: 4304 5460 4304
Size: 1156 5091
ApoI r'AATT_y
Cuts at: 1493 3556 3782 3934 6043 1493
Size: 2063 226 152 2109 1697
Fragments arranged by size:
2109 2063 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4801 160
Size: 872 3769 1606
Fragments arranged by size:
3769 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6247
AvaI C'yCGr_G
Cuts at: 1870 1943 2092 3572 1870
Size: 73 149 1480 4545
Fragments arranged by size:
4545 1480 149 73
BamHI G'GATC_C
Cuts at: 3577 3944 3577
Size: 367 5880
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3568 5023 618
Size: 325 217 627 1781 1455 1842
Fragments arranged by size:
1842 1781 1455 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2924 3161 3566 1391
Size: 1533 237 405 4072
Fragments arranged by size:
4072 1533 405 237
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 5276
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2176 2210 4438 4472 1903
Size: 34 239 34 2228 34 3678
Fragments arranged by size:
3678 2228 239 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4139 5349 4139
Size: 1210 5037
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6247
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2567 4858 136
Size: 107 122 71 631 605 895 2291 1525
Fragments arranged by size:
2291 1525 895 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6242 6242
Size: 6247
BlpI GC'TnA_GC
Cuts at: 2723 3664 2723
Size: 941 5306
Bme1580I G_kGCm'C
Cuts at: 1790 4308 5464 1790
Size: 2518 1156 2573
Fragments arranged by size:
2573 2518 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6247
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3978 4936 442
Size: 416 53 83 399 24 2561 958 1753
Fragments arranged by size:
2561 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6247
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2938 3199 2938
Size: 261 5986
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 3161 3347 4907 1239
Size: 1922 186 1560 2579
Fragments arranged by size:
2579 1922 1560 186
Bpu10I CC'TnA_GC
Cuts at: 3160 3160
Size: 6247
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4372 5237 5535 4372
Size: 865 298 5084
Fragments arranged by size:
5084 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3187 4910 882
Size: 617 1688 1723 2219
Fragments arranged by size:
2219 1723 1688 617
BsaAI yAC'GTr
Cuts at: 493 1204 4038 493
Size: 711 2834 2702
Fragments arranged by size:
2834 2702 711
BsaBI GATnn'nnATC
Cuts at: 6241 6241
Size: 6247
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3501 4054 4436 5071
Size: 53 83 186 936 1968 553 382 635
Cuts at: 5071 275
Size: 1451
Fragments arranged by size:
1968 1451 936 635 553 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 4680 5078 5352 5499 5910 4680
Size: 398 274 147 411 5017
Fragments arranged by size:
5017 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 3134 3164 1178
Size: 30 165 30 1731 30 4261
Fragments arranged by size:
4261 1731 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2318 3065 1186
Size: 1132 747 4368
Fragments arranged by size:
4368 1132 747
BseYI C'CCAG_C
Cuts at: 2050 2570 5450 5946 2050
Size: 520 2880 496 2351
Fragments arranged by size:
2880 2351 520 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2405 2825 1163
Size: 573 669 420 4585
Fragments arranged by size:
4585 669 573 420
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2864 3697 4458 5486 1540
Size: 275 186 863 833 761 1028 2301
Fragments arranged by size:
2301 1028 863 833 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 4308 4393 5464 4308
Size: 85 1071 5091
Fragments arranged by size:
5091 1071 85
BsmI GAATG_Cn'
Cuts at: 3758 3851 3758
Size: 93 6154
BsmBI CGTCTCn'nnnn_
Cuts at: 2060 3492 2060
Size: 1432 4815
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2924 3161 3566 4308 4393 5464 1391
Size: 399 1134 237 405 742 85 1071 2174
Fragments arranged by size:
2174 1134 1071 742 405 399 237 85
BspEI T'CCGG_A
Cuts at: 5910 5910
Size: 6247
BspHI T'CATG_A
Cuts at: 2688 4136 5866 2688
Size: 1448 1730 3069
Fragments arranged by size:
3069 1730 1448
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2880 2909 3602 844
Size: 2036 29 693 3489
Fragments arranged by size:
3489 2036 693 29
BsrBI CCG'CTC
Cuts at: 1394 1823 2365 2386 4134 1394
Size: 429 542 21 1748 3507
Fragments arranged by size:
3507 1748 542 429 21
BsrDI GCAATG_nn'
Cuts at: 65 1248 2829 4742 4916 65
Size: 1183 1581 1913 174 1396
Fragments arranged by size:
1913 1581 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 3216 4891 5078 1700
Size: 110 1406 1675 187 2869
Fragments arranged by size:
2869 1675 1406 187 110
BsrGI T'GTAC_A
Cuts at: 96 2489 96
Size: 2393 3854
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6247
BssSI C'ACGA_G
Cuts at: 4307 5319 4307
Size: 1012 5235
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6247
BstBI TT'CG_AA
Cuts at: 3223 5051 3223
Size: 1828 4419
BstEII G'GTnAC_C
Cuts at: 1184 3535 1184
Size: 2351 3896
BstXI CCAn_nnnn'nTGG
Cuts at: 2011 2011
Size: 6247
BstYI r'GATC_y
Cuts at: 1972 2014 3577 3944 4329 4346 5798 5884 5896
Size: 42 1563 367 385 17 1452 86 12
Cuts at: 5896 6242 1972
Size: 346 1977
Fragments arranged by size:
1977 1563 1452 385 367 346 86 42
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2562 2926 2941 513
Size: 552 939 558 364 15 3819
Fragments arranged by size:
3819 939 558 552 364 15
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5508
Fragments arranged by size:
5508 683 56
BtsI GCAGTG_nn'
Cuts at: 2773 3763 4557 4577 2773
Size: 990 794 20 4443
Fragments arranged by size:
4443 990 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6247
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6212
DraI TTT'AAA
Cuts at: 3551 3551
Size: 6247
DraIII CAC_nnn'GTG
Cuts at: 3088 3088
Size: 6247
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5254 809
Size: 507 3938 1802
Fragments arranged by size:
3938 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2861 2929 3694
Size: 54 1425 50 275 18 1031 68 765
Cuts at: 3694 4583 6212 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 1031 889 765 275 68 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2861 3694 1537
Size: 275 1049 833 4090
Fragments arranged by size:
4090 1049 833 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3024 4177 1353
Size: 1671 1153 3423
Fragments arranged by size:
3423 1671 1153
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4834 5208 5354 387
Size: 1064 138 3245 374 146 1280
Fragments arranged by size:
3245 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2192 2369 3161 3347 4310 4907 5694
Size: 393 560 177 792 186 963 597 787
Cuts at: 5694 5962 1239
Size: 268 1524
Fragments arranged by size:
1524 963 792 787 597 560 393 268
186 177
EcoNI CCTnn'n_nnAGG
Cuts at: 2615 2615
Size: 6247
EcoO109I rG'GnC_Cy
Cuts at: 2047 2089 2599 2869 2047
Size: 42 510 270 5425
Fragments arranged by size:
5425 510 270 42
EcoRI G'AATT_C
Cuts at: 3556 3934 3556
Size: 378 5869
FspI TGC'GCA
Cuts at: 4753 4753
Size: 6247
HaeII r_GCGC'y
Cuts at: 1678 2083 5394 1678
Size: 405 3311 2531
Fragments arranged by size:
3311 2531 405
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2222 2254 2771 2803 2996
Size: 32 135 32 650 32 517 32 193
Cuts at: 2996 3028 4888 4920 4962 4994 1373
Size: 32 1860 32 42 32 2626
Fragments arranged by size:
2626 1860 650 517 193 135 42 32
32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3591 3837 669
Size: 988 281 1653 246 3079
Fragments arranged by size:
3079 1653 988 281 246
HindIII A'AGCT_T
Cuts at: 748 3131 748
Size: 2383 3864
HpaI GTT'AAC
Cuts at: 3837 3837
Size: 6247
KpnI G_GTAC'C
Cuts at: 3572 3572
Size: 6247
MfeI C'AATT_G
Cuts at: 3846 3846
Size: 6247
MluI A'CGCG_T
Cuts at: 6123 6123
Size: 6247
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2347 2355 5361 5545 1305
Size: 1042 8 3006 184 2007
Fragments arranged by size:
3006 2007 1042 184 8
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4425
Fragments arranged by size:
4425 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2847 2931 3356 4205 4564 4723 518
Size: 648 1681 84 425 849 359 159 2042
Fragments arranged by size:
2042 1681 849 648 425 359 159 84
MspA1I CmG'CkG
Cuts at: 1606 2110 3663 4340 5488 5733 1606
Size: 504 1553 677 1148 245 2120
Fragments arranged by size:
2120 1553 1148 677 504 245
NaeI GCC'GGC
Cuts at: 3218 3218
Size: 6247
NcoI C'CATG_G
Cuts at: 513 1065 2004 2926 513
Size: 552 939 922 3834
Fragments arranged by size:
3834 939 922 552
NdeI CA'TA_TG
Cuts at: 387 3276 387
Size: 2889 3358
NgoMIV G'CCGG_C
Cuts at: 3216 3216
Size: 6247
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6247
NotI GC'GGCC_GC
Cuts at: 3694 3694
Size: 6247
NspI r_CATG'y
Cuts at: 1132 1229 3605 5150 1132
Size: 97 2376 1545 2229
Fragments arranged by size:
2376 2229 1545 97
PasI CC'CwG_GG
Cuts at: 1348 3067 1348
Size: 1719 4528
PciI A'CATG_T
Cuts at: 5146 5146
Size: 6247
PflMI CCAn_nnn'nTGG
Cuts at: 1108 3067 1108
Size: 1959 4288
PfoI T'CCnGG_A
Cuts at: 2610 3324 2610
Size: 714 5533
PmeI GTTT'AAAC
Cuts at: 3551 3551
Size: 6247
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2607 2639 4316 4348 5829 5861 2607
Size: 32 1677 32 1481 32 2993
Fragments arranged by size:
2993 1677 1481 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 2047 2089 2599 2869 2047
Size: 42 510 270 5425
Fragments arranged by size:
5425 510 270 42
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6247
PsiI TTA'TAA
Cuts at: 2748 3817 2748
Size: 1069 5178
PstI C_TGCA'G
Cuts at: 830 2281 3525 3599 830
Size: 1451 1244 74 3478
Fragments arranged by size:
3478 1451 1244 74
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6247
PvuII CAG'CTG
Cuts at: 2110 2110
Size: 6247
SalI G'TCGA_C
Cuts at: 1936 3589 1936
Size: 1653 4594
SanDI GG'GwC_CC
Cuts at: 2047 2047
Size: 6247
SbfI CC_TGCA'GG
Cuts at: 3599 3599
Size: 6247
ScaI AGT'ACT
Cuts at: 2507 4495 6207 2507
Size: 1988 1712 2547
Fragments arranged by size:
2547 1988 1712
SexAI A'CCwGG_T
Cuts at: 2520 2520
Size: 6247
SfcI C'TryA_G
Cuts at: 826 1045 2277 3521 3595 4730 5411 5602 826
Size: 219 1232 1244 74 1135 681 191 1471
Fragments arranged by size:
1471 1244 1232 1135 681 219 191 74
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6247
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6247
SmaI CCC'GGG
Cuts at: 1872 2094 3574 1872
Size: 222 1480 4545
Fragments arranged by size:
4545 1480 222
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4351 5252 5514 820
Size: 197 926 2408 901 262 1553
Fragments arranged by size:
2408 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 4038 493
Size: 3545 2702
SpeI A'CTAG_T
Cuts at: 152 2098 152
Size: 1946 4301
SphI G_CATG'C
Cuts at: 1132 3605 1132
Size: 2473 3774
SspI AAT'ATT
Cuts at: 5 52 3094 4171 5
Size: 47 3042 1077 2081
Fragments arranged by size:
3042 2081 1077 47
StuI AGG'CCT
Cuts at: 1517 3146 1517
Size: 1629 4618
StyI C'CwwG_G
Cuts at: 513 1065 2004 2646 2926 3686 513
Size: 552 939 642 280 760 3074
Fragments arranged by size:
3074 939 760 642 552 280
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 4274 4291 4444 4629 5074 325
Size: 1255 2694 17 153 185 445 1498
Fragments arranged by size:
2694 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2489 2505 2988
Size: 274 80 33 51 1443 512 16 483
Cuts at: 2988 4493 6205 96
Size: 1505 1712 138
Fragments arranged by size:
1712 1505 1443 512 483 274 138 80
51 33 16
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2467 2499 6207 6239 2467
Size: 32 3708 32 2475
Fragments arranged by size:
3708 2475 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6247
XbaI T'CTAG_A
Cuts at: 3583 3583
Size: 6247
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3250 3250
Size: 6247
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6247
XmaI C'CCGG_G
Cuts at: 1870 2092 3572 1870
Size: 222 1480 4545
Fragments arranged by size:
4545 1480 222
XmnI GAAnn'nnTTC
Cuts at: 2081 3377 4376 2081
Size: 1296 999 3952
Fragments arranged by size:
3952 1296 999
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3502 4055 276
Size: 53 83 186 936 1968 553 2468
Fragments arranged by size:
2468 1968 936 553 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI
BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI
EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI
FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI
MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI
NgoMIV NheI NotI NspI PasI PciI PflMI PfoI
PmeI PpiI PpuMI PshAI PsiI PstI PvuI PvuII
SalI SanDI SbfI ScaI SexAI SfcI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AarI AloI ApaI AscI AvrII BaeI BbeI BbvCI
BfrBI BsiWI BstZ17I Bsu36I EcoICRI EcoRV FalI FseI
FspAI KasI NarI NruI NsiI PacI PmlI PspOMI
PspXI PsrI RsrII SacI SacII SapI SfiI SfoI
SrfI SwaI