Restriction map for
pFN21AB5451
(Circular) (Six-base) MAPSORT of: pFN21AB5451.seq Check: 1130 from: 1 to: 5821
pFN21AB5451 5821 bp
With 182 enzymes: SgfI *
April 15, 2010 09:31 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2051 2051
Size: 5821
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 2474 3631 278
Size: 53 83 186 936 938 1157 2468
Fragments arranged by size:
2468 1157 938 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2145 3164 1937
Size: 208 1019 4594
Fragments arranged by size:
4594 1019 208
Acc65I G'GTAC_C
Cuts at: 3142 3142
Size: 5821
AclI AA'CG_TT
Cuts at: 1563 3948 4321 1563
Size: 2385 373 3063
Fragments arranged by size:
3063 2385 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2120 2550 3062 3884 5268 5536 1632
Size: 488 430 512 822 1384 268 1917
Fragments arranged by size:
1917 1384 822 512 488 430 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5624
AflIII A'CryG_T
Cuts at: 1203 3048 4720 5697 1203
Size: 1845 1672 977 1327
Fragments arranged by size:
1845 1672 1327 977
AgeI A'CCGG_T
Cuts at: 4652 4652
Size: 5821
AhdI GACnn_n'nnGTC
Cuts at: 4550 4550
Size: 5821
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5821
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 3107 1184
Size: 1923 3898
AlwNI CAG_nnn'CTG
Cuts at: 2593 2961 3564 5136 2593
Size: 368 603 1572 3278
Fragments arranged by size:
3278 1572 603 368
ApaLI G'TGCA_C
Cuts at: 3878 5034 3878
Size: 1156 4665
ApoI r'AATT_y
Cuts at: 1493 2181 2328 2518 2816 3130 3356 3508 5617
Size: 688 147 190 298 314 226 152 2109
Cuts at: 5617 1493
Size: 1697
Fragments arranged by size:
2109 1697 688 314 298 226 190 152
147
AseI AT'TA_AT
Cuts at: 160 1032 4375 160
Size: 872 3343 1606
Fragments arranged by size:
3343 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5821
AvaI C'yCGr_G
Cuts at: 1870 1943 2236 3025 3146 1870
Size: 73 293 789 121 4545
Fragments arranged by size:
4545 789 293 121 73
BamHI G'GATC_C
Cuts at: 3151 3518 3151
Size: 367 5454
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2135 3142 4597 618
Size: 325 217 627 348 1007 1455 1842
Fragments arranged by size:
1842 1455 1007 627 348 325 217
BanII G_rGCy'C
Cuts at: 1391 2277 2852 3140 1391
Size: 886 575 288 4072
Fragments arranged by size:
4072 886 575 288
BbeI G_GCGC'C
Cuts at: 2139 2139
Size: 5821
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2556 928
Size: 971 657 4193
Fragments arranged by size:
4193 971 657
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4012 4046 1903
Size: 34 2075 34 3678
Fragments arranged by size:
3678 2075 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3713 4923 3713
Size: 1210 4611
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5821
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2625 4432 136
Size: 107 122 71 631 605 953 1807 1525
Fragments arranged by size:
1807 1525 953 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5816 5816
Size: 5821
BlpI GC'TnA_GC
Cuts at: 3238 3238
Size: 5821
Bme1580I G_kGCm'C
Cuts at: 1790 3882 5038 1790
Size: 2092 1156 2573
Fragments arranged by size:
2573 2092 1156
BmgBI CAC'GTC
Cuts at: 1318 2424 1318
Size: 1106 4715
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3552 4510 442
Size: 416 53 83 399 24 2135 958 1753
Fragments arranged by size:
2135 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5821
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 4481 1239
Size: 3242 2579
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3946 4811 5109 3946
Size: 865 298 4658
Fragments arranged by size:
4658 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2873 4484 882
Size: 617 1374 1611 2219
Fragments arranged by size:
2219 1611 1374 617
BsaAI yAC'GTr
Cuts at: 493 1204 3091 3612 493
Size: 711 1887 521 2702
Fragments arranged by size:
2702 1887 711 521
BsaBI GATnn'nnATC
Cuts at: 5815 5815
Size: 5821
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2136 2316 2471 3114
Size: 53 83 186 936 603 180 155 643
Cuts at: 3114 3628 4010 4645 275
Size: 514 382 635 1451
Fragments arranged by size:
1451 936 643 635 603 514 382 186
180 155 83 53
BsaWI w'CCGG_w
Cuts at: 2121 4254 4652 4926 5073 5484 2121
Size: 2133 398 274 147 411 2458
Fragments arranged by size:
2458 2133 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5596
Fragments arranged by size:
5596 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2285 1186
Size: 1099 4722
BseYI C'CCAG_C
Cuts at: 2711 2948 5024 5520 2711
Size: 237 2076 496 3012
Fragments arranged by size:
3012 2076 496 237
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 3113 1163
Size: 573 1377 3871
Fragments arranged by size:
3871 1377 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3271 4032 5060 1540
Size: 275 186 1270 761 1028 2301
Fragments arranged by size:
2301 1270 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2277 3882 3967 5038 2277
Size: 1605 85 1071 3060
Fragments arranged by size:
3060 1605 1071 85
BsmI GAATG_Cn'
Cuts at: 3332 3425 3332
Size: 93 5728
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2277 2852 3140 3882 3967 5038 1391
Size: 399 487 575 288 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 575 487 399 288 85
BspEI T'CCGG_A
Cuts at: 5484 5484
Size: 5821
BspHI T'CATG_A
Cuts at: 3710 5440 3710
Size: 1730 4091
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2051 3176 844
Size: 1207 1125 3489
Fragments arranged by size:
3489 1207 1125
BsrBI CCG'CTC
Cuts at: 1394 1823 3708 1394
Size: 429 1885 3507
Fragments arranged by size:
3507 1885 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2206 4316 4490 65
Size: 1183 958 2110 174 1396
Fragments arranged by size:
2110 1396 1183 958 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2195 4465 4652 1700
Size: 110 385 2270 187 2869
Fragments arranged by size:
2869 2270 385 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5821
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5821
BssSI C'ACGA_G
Cuts at: 3881 4893 3881
Size: 1012 4809
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2961 794
Size: 2167 3654
BstBI TT'CG_AA
Cuts at: 4625 4625
Size: 5821
BstEII G'GTnAC_C
Cuts at: 1184 2989 1184
Size: 1805 4016
BstXI CCAn_nnnn'nTGG
Cuts at: 2638 2801 2638
Size: 163 5658
BstYI r'GATC_y
Cuts at: 1972 3151 3518 3903 3920 5372 5458 5470 5816
Size: 1179 367 385 17 1452 86 12 346
Cuts at: 5816 1972
Size: 1977
Fragments arranged by size:
1977 1452 1179 385 367 346 86 17
12
BtgI C'CryG_G
Cuts at: 513 1065 2465 513
Size: 552 1400 3869
Fragments arranged by size:
3869 1400 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2287 487
Size: 683 56 1061 4021
Fragments arranged by size:
4021 1061 683 56
BtsI GCAGTG_nn'
Cuts at: 3337 4131 4151 3337
Size: 794 20 5007
Fragments arranged by size:
5007 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5821
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5786
DraI TTT'AAA
Cuts at: 3125 3125
Size: 5821
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4828 809
Size: 507 3512 1802
Fragments arranged by size:
3512 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2193 2346 3102
Size: 54 1425 50 275 18 363 153 756
Cuts at: 3102 3268 4157 5786 8
Size: 166 889 1629 43
Fragments arranged by size:
1629 1425 889 756 363 275 166 153
54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3268 1537
Size: 275 1456 4090
Fragments arranged by size:
4090 1456 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2871 3068 3751 1353
Size: 1518 197 683 3423
Fragments arranged by size:
3423 1518 683 197
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2921 4408 4782 4928 387
Size: 1064 138 1332 1487 374 146 1280
Fragments arranged by size:
1487 1332 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2275 2275
Size: 5821
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2120 2550 3062 3884 4481 5268 5536
Size: 393 488 430 512 822 597 787 268
Cuts at: 5536 1239
Size: 1524
Fragments arranged by size:
1524 822 787 597 512 488 430 393
268
EcoO109I rG'GnC_Cy
Cuts at: 2762 2762
Size: 5821
EcoRI G'AATT_C
Cuts at: 2328 3130 3508 2328
Size: 802 378 4641
Fragments arranged by size:
4641 802 378
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2614 2888 2614
Size: 274 5547
FspI TGC'GCA
Cuts at: 4327 4327
Size: 5821
HaeII r_GCGC'y
Cuts at: 1678 2139 4968 1678
Size: 461 2829 2531
Fragments arranged by size:
2829 2531 461
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2080 2112 2286 2318 4462
Size: 32 135 32 508 32 174 32 2144
Cuts at: 4462 4494 4536 4568 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 2144 508 174 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3165 3411 669
Size: 988 281 1227 246 3079
Fragments arranged by size:
3079 1227 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5821
HpaI GTT'AAC
Cuts at: 3411 3411
Size: 5821
KasI G'GCGC_C
Cuts at: 2135 2135
Size: 5821
KpnI G_GTAC'C
Cuts at: 3146 3146
Size: 5821
MfeI C'AATT_G
Cuts at: 3420 3420
Size: 5821
MluI A'CGCG_T
Cuts at: 5697 5697
Size: 5821
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2355 2877 4935 5119 1305
Size: 1050 522 2058 184 2007
Fragments arranged by size:
2058 2007 1050 522 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2348 3104 10
Size: 54 1425 343 516 756 2727
Fragments arranged by size:
2727 1425 756 516 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2636 3018 3089 3779 4138 4297 518
Size: 648 1470 382 71 690 359 159 2042
Fragments arranged by size:
2042 1470 690 648 382 359 159 71
MspA1I CmG'CkG
Cuts at: 1606 2177 2284 2903 2952 3237 3914 5062 5307
Size: 571 107 619 49 285 677 1148 245
Cuts at: 5307 1606
Size: 2120
Fragments arranged by size:
2120 1148 677 619 571 285 245 107
49
NaeI GCC'GGC
Cuts at: 2197 2197
Size: 5821
NarI GG'CG_CC
Cuts at: 2136 2136
Size: 5821
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5269
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5821
NgoMIV G'CCGG_C
Cuts at: 2195 2195
Size: 5821
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5821
NotI GC'GGCC_GC
Cuts at: 3268 3268
Size: 5821
NspI r_CATG'y
Cuts at: 1132 1229 3179 4724 1132
Size: 97 1950 1545 2229
Fragments arranged by size:
2229 1950 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5821
PciI A'CATG_T
Cuts at: 4720 4720
Size: 5821
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2546 1108
Size: 1438 4383
PmeI GTTT'AAAC
Cuts at: 3125 3125
Size: 5821
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3890 3922 5403 5435 3890
Size: 32 1481 32 4276
Fragments arranged by size:
4276 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5821
PsiI TTA'TAA
Cuts at: 3391 3391
Size: 5821
PspXI vC'TCGA_Gb
Cuts at: 3025 3025
Size: 5821
PstI C_TGCA'G
Cuts at: 830 3173 830
Size: 2343 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5821
PvuII CAG'CTG
Cuts at: 2284 2284
Size: 5821
SacI G_AGCT'C
Cuts at: 2277 2277
Size: 5821
SalI G'TCGA_C
Cuts at: 1936 3163 1936
Size: 1227 4594
SapI GCTCTTCn'nnn_
Cuts at: 3068 3068
Size: 5821
SbfI CC_TGCA'GG
Cuts at: 3173 3173
Size: 5821
ScaI AGT'ACT
Cuts at: 4069 5781 4069
Size: 1712 4109
SfcI C'TryA_G
Cuts at: 826 1045 3169 4304 4985 5176 826
Size: 219 2124 1135 681 191 1471
Fragments arranged by size:
2124 1471 1135 681 219 191
SfoI GGC'GCC
Cuts at: 2137 2137
Size: 5821
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5821
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5821
SmaI CCC'GGG
Cuts at: 1872 3148 1872
Size: 1276 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3025 3925 4826 5088 820
Size: 197 926 1082 900 901 262 1553
Fragments arranged by size:
1553 1082 926 901 900 262 197
SnaBI TAC'GTA
Cuts at: 493 3612 493
Size: 3119 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5821
SphI G_CATG'C
Cuts at: 1132 3179 1132
Size: 2047 3774
SspI AAT'ATT
Cuts at: 5 52 3745 5
Size: 47 3693 2081
Fragments arranged by size:
3693 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5821
StyI C'CwwG_G
Cuts at: 513 1065 2451 2700 2758 3117 3260 513
Size: 552 1386 249 58 359 143 3074
Fragments arranged by size:
3074 1386 552 359 249 143 58
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2670 3848 3865 4018 4203 4648 325
Size: 1255 1090 1178 17 153 185 445 1498
Fragments arranged by size:
1498 1255 1178 1090 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3051 4067 5779
Size: 274 80 33 51 1443 1074 1016 1712
Cuts at: 5779 96
Size: 138
Fragments arranged by size:
1712 1443 1074 1016 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5781 5813 5781
Size: 32 5789
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5821
XbaI T'CTAG_A
Cuts at: 2185 3157 2185
Size: 972 4849
XhoI C'TCGA_G
Cuts at: 1943 3025 1943
Size: 1082 4739
XmaI C'CCGG_G
Cuts at: 1870 3146 1870
Size: 1276 4545
XmnI GAAnn'nnTTC
Cuts at: 3950 3950
Size: 5821
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 2472 3629 276
Size: 53 83 186 936 938 1157 2468
Fragments arranged by size:
2468 1157 938 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI
BtsI ClaI CspCI DraI DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII
Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI
MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI
NgoMIV NheI NotI NspI PasI PciI PflMI PmeI
PpiI PshAI PsiI PspXI PstI PvuI PvuII SacI
SalI SapI SbfI ScaI SfcI SfoI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AfeI AloI ApaI AscI AvrII BaeI BbvCI BfrBI
BplI Bpu10I BsiWI BsmBI BstZ17I Bsu36I DraIII EcoNI
EcoRV FseI FspAI NruI NsiI PacI PfoI PmlI
PpuMI PspOMI PsrI RsrII SacII SanDI SexAI SfiI
SrfI SwaI XcmI