Restriction map for
pFN21AB5446
(Circular) (Six-base) MAPSORT of: pFN21AB5446.seq Check: 4247 from: 1 to: 6670
pFN21AB5446 6670 bp
With 182 enzymes: SgfI *
October 20, 2009 10:36 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4480 278
Size: 53 83 186 936 2944 2468
Fragments arranged by size:
2944 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 4013 1937
Size: 2076 4594
Acc65I G'GTAC_C
Cuts at: 3053 3991 3053
Size: 938 5732
AclI AA'CG_TT
Cuts at: 1563 4797 5170 1563
Size: 3234 373 3063
Fragments arranged by size:
3234 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 4733 6117 6385 1632
Size: 3101 1384 268 1917
Fragments arranged by size:
3101 1917 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6473
AflIII A'CryG_T
Cuts at: 1203 5569 6546 1203
Size: 4366 977 1327
Fragments arranged by size:
4366 1327 977
AgeI A'CCGG_T
Cuts at: 5501 5501
Size: 6670
AhdI GACnn_n'nnGTC
Cuts at: 5399 5399
Size: 6670
AleI CACnn'nnGTG
Cuts at: 1166 3641 1166
Size: 2475 4195
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6670
AlwNI CAG_nnn'CTG
Cuts at: 2047 2712 3694 3706 4413 5985 2047
Size: 665 982 12 707 1572 2732
Fragments arranged by size:
2732 1572 982 707 665 12
ApaI G_GGCC'C
Cuts at: 2545 2889 2545
Size: 344 6326
ApaLI G'TGCA_C
Cuts at: 4727 5883 4727
Size: 1156 5514
ApoI r'AATT_y
Cuts at: 1493 3120 3979 4205 4357 6466 1493
Size: 1627 859 226 152 2109 1697
Fragments arranged by size:
2109 1697 1627 859 226 152
AseI AT'TA_AT
Cuts at: 160 1032 5224 160
Size: 872 4192 1606
Fragments arranged by size:
4192 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6670
AvaI C'yCGr_G
Cuts at: 1870 1943 2054 3751 3995 1870
Size: 73 111 1697 244 4545
Fragments arranged by size:
4545 1697 244 111 73
BamHI G'GATC_C
Cuts at: 3843 4000 4367 3843
Size: 157 367 6146
Fragments arranged by size:
6146 367 157
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2132 2953 3053 3991 5446
Size: 325 217 627 345 821 100 938 1455
Cuts at: 5446 618
Size: 1842
Fragments arranged by size:
1842 1455 938 821 627 345 325 217
100
BanII G_rGCy'C
Cuts at: 1391 2545 2661 2889 3456 3622 3894 3989 1391
Size: 1154 116 228 567 166 272 95 4072
Fragments arranged by size:
4072 1154 567 272 228 166 116 95
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 5699
BbvCI CC'TCA_GC
Cuts at: 2048 3530 2048
Size: 1482 5188
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 1997 2021 2031 3713 3747 4861
Size: 34 50 10 24 10 1682 34 1114
Cuts at: 4861 4895 1903
Size: 34 3678
Fragments arranged by size:
3678 1682 1114 50 34 34 34 24
10 10
BciVI GTATCCnnnnn_n'
Cuts at: 4562 5772 4562
Size: 1210 5460
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6670
BfrBI ATG'CAT
Cuts at: 2512 3303 2512
Size: 791 5879
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2009 5281 136
Size: 107 122 71 631 605 337 3272 1525
Fragments arranged by size:
3272 1525 631 605 337 122 107 71
BglII A'GATC_T
Cuts at: 3364 6665 3364
Size: 3301 3369
BlpI GC'TnA_GC
Cuts at: 2139 4087 2139
Size: 1948 4722
Bme1580I G_kGCm'C
Cuts at: 1790 2445 2545 2706 2889 4731 5887 1790
Size: 655 100 161 183 1842 1156 2573
Fragments arranged by size:
2573 1842 1156 655 183 161 100
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6670
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2439 2473 3100
Size: 416 53 83 399 24 1022 34 627
Cuts at: 3100 4401 5359 442
Size: 1301 958 1753
Fragments arranged by size:
1753 1301 1022 958 627 416 399 83
53 34 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6670
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2399 3363 3644 5330 1239
Size: 1160 964 281 1686 2579
Fragments arranged by size:
2579 1686 1160 964 281
Bpu10I CC'TnA_GC
Cuts at: 2048 3075 3530 2048
Size: 1027 455 5188
Fragments arranged by size:
5188 1027 455
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2396 2647 3745 4795 5660 5958 2396
Size: 251 1098 1050 865 298 3108
Fragments arranged by size:
3108 1098 1050 865 298 251
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2387 5333 882
Size: 617 888 2946 2219
Fragments arranged by size:
2946 2219 888 617
BsaAI yAC'GTr
Cuts at: 493 1204 4461 493
Size: 711 3257 2702
Fragments arranged by size:
3257 2702 711
BsaBI GATnn'nnATC
Cuts at: 2906 6664 2906
Size: 3758 2912
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4477 4859 5494 275
Size: 53 83 186 936 2944 382 635 1451
Fragments arranged by size:
2944 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 3378 5103 5501 5775 5922 6333 3378
Size: 1725 398 274 147 411 3715
Fragments arranged by size:
3715 1725 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2315 2345 2937 2967 1178
Size: 30 165 30 912 30 592 30 4881
Fragments arranged by size:
4881 912 592 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2405 1186
Size: 1219 5451
BseYI C'CCAG_C
Cuts at: 2419 2452 2705 2971 3547 3792 5873 6369 2419
Size: 33 253 266 576 245 2081 496 2720
Fragments arranged by size:
2720 2081 576 496 266 253 245 33
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 6097
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4120 4881 5909 1540
Size: 275 186 2119 761 1028 2301
Fragments arranged by size:
2301 2119 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2286 3360 3456 3475 3894 4731 4816 5887 2286
Size: 1074 96 19 419 837 85 1071 3069
Fragments arranged by size:
3069 1074 1071 837 419 96 85 19
BsmI GAATG_Cn'
Cuts at: 3305 3499 4181 4274 3305
Size: 194 682 93 5701
Fragments arranged by size:
5701 682 194 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2286 2445 2545 2661 2706 2889 3360
Size: 399 496 159 100 116 45 183 471
Cuts at: 3360 3456 3475 3622 3894 3989 4731 4816 5887
Size: 96 19 147 272 95 742 85 1071
Cuts at: 5887 1391
Size: 2174
Fragments arranged by size:
2174 1071 742 496 471 399 272 183
159 147 116 100 96 95 85 45
19
BspEI T'CCGG_A
Cuts at: 3378 6333 3378
Size: 2955 3715
BspHI T'CATG_A
Cuts at: 3330 4559 6289 3330
Size: 1229 1730 3711
Fragments arranged by size:
3711 1730 1229
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3041 4025 844
Size: 2197 984 3489
Fragments arranged by size:
3489 2197 984
BsrBI CCG'CTC
Cuts at: 1394 1823 2138 3934 4557 1394
Size: 429 315 1796 623 3507
Fragments arranged by size:
3507 1796 623 429 315
BsrDI GCAATG_nn'
Cuts at: 65 1248 3069 5165 5339 65
Size: 1183 1821 2096 174 1396
Fragments arranged by size:
2096 1821 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2197 5314 5501 1700
Size: 110 387 3117 187 2869
Fragments arranged by size:
3117 2869 387 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6670
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6670
BssSI C'ACGA_G
Cuts at: 4730 5742 4730
Size: 1012 5658
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6670
BstBI TT'CG_AA
Cuts at: 5474 5474
Size: 6670
BstEII G'GTnAC_C
Cuts at: 1184 3946 1184
Size: 2762 3908
BstXI CCAn_nnnn'nTGG
Cuts at: 2452 3643 3742 3925 2452
Size: 1191 99 183 5197
Fragments arranged by size:
5197 1191 183 99
BstYI r'GATC_y
Cuts at: 1972 2809 3364 3381 3764 3843 4000 4367 4752
Size: 837 555 17 383 79 157 367 385
Cuts at: 4752 4769 6221 6307 6319 6665 1972
Size: 17 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 837 555 385 383 367 346
157 86 79 17 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2881 3189 3228 3735 3786 513
Size: 552 1816 308 39 507 51 3397
Fragments arranged by size:
3397 1816 552 507 308 51 39
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5931
Fragments arranged by size:
5931 683 56
BtsI GCAGTG_nn'
Cuts at: 3475 4186 4980 5000 3475
Size: 711 794 20 5145
Fragments arranged by size:
5145 794 711 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6670
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6635
DraI TTT'AAA
Cuts at: 3974 3974
Size: 6670
DraIII CAC_nnn'GTG
Cuts at: 3822 3822
Size: 6670
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5677 809
Size: 507 4361 1802
Fragments arranged by size:
4361 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2022 2199 2637
Size: 54 1425 50 275 18 192 177 438
Cuts at: 2637 3858 4117 5006 6635 8
Size: 1221 259 889 1629 43
Fragments arranged by size:
1629 1425 1221 889 438 275 259 192
177 54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 4117 1537
Size: 275 2305 4090
Fragments arranged by size:
4090 2305 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2286 4600 1353
Size: 933 2314 3423
Fragments arranged by size:
3423 2314 933
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2076 2222 5257 5631 5777 387
Size: 1064 138 487 146 3035 374 146 1280
Fragments arranged by size:
3035 1280 1064 487 374 146 146 138
EcoICRI GAG'CTC
Cuts at: 3454 3892 3454
Size: 438 6232
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2399 3363 3644 4733 5330 6117 6385
Size: 393 767 964 281 1089 597 787 268
Cuts at: 6385 1239
Size: 1524
Fragments arranged by size:
1524 1089 964 787 767 597 393 281
268
EcoNI CCTnn'n_nnAGG
Cuts at: 3773 3773
Size: 6670
EcoO109I rG'GnC_Cy
Cuts at: 2407 2424 2541 2764 2886 3756 2407
Size: 17 117 223 122 870 5321
Fragments arranged by size:
5321 870 223 122 117 17
EcoRI G'AATT_C
Cuts at: 3979 4357 3979
Size: 378 6292
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3502 3502
Size: 6670
FspI TGC'GCA
Cuts at: 5176 5176
Size: 6670
HaeII r_GCGC'y
Cuts at: 1678 2084 5817 1678
Size: 406 3733 2531
Fragments arranged by size:
3733 2531 406
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2315 2347 2666 2698 5311
Size: 32 135 32 743 32 319 32 2613
Cuts at: 5311 5343 5385 5417 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 2613 743 319 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 4014 4260 669
Size: 988 281 2076 246 3079
Fragments arranged by size:
3079 2076 988 281 246
HindIII A'AGCT_T
Cuts at: 748 2744 748
Size: 1996 4674
HpaI GTT'AAC
Cuts at: 4260 4260
Size: 6670
KpnI G_GTAC'C
Cuts at: 3057 3995 3057
Size: 938 5732
MfeI C'AATT_G
Cuts at: 4269 4269
Size: 6670
MluI A'CGCG_T
Cuts at: 6546 6546
Size: 6670
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3575 5784 5968 1305
Size: 2270 2209 184 2007
Fragments arranged by size:
2270 2209 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4848
Fragments arranged by size:
4848 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3641 3740 4628 4987 5146 518
Size: 648 2475 99 888 359 159 2042
Fragments arranged by size:
2475 2042 888 648 359 159 99
MspA1I CmG'CkG
Cuts at: 1606 2143 3529 3555 3570 3703 4086 4763 5911
Size: 537 1386 26 15 133 383 677 1148
Cuts at: 5911 6156 1606
Size: 245 2120
Fragments arranged by size:
2120 1386 1148 677 537 383 245 133
26 15
NcoI C'CATG_G
Cuts at: 513 1065 2881 3189 3735 513
Size: 552 1816 308 546 3448
Fragments arranged by size:
3448 1816 552 546 308
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6670
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6670
NotI GC'GGCC_GC
Cuts at: 4117 4117
Size: 6670
NsiI A_TGCA'T
Cuts at: 2514 3305 2514
Size: 791 5879
NspI r_CATG'y
Cuts at: 1132 1229 2512 4028 5573 1132
Size: 97 1283 1516 1545 2229
Fragments arranged by size:
2229 1545 1516 1283 97
PasI CC'CwG_GG
Cuts at: 1348 2536 1348
Size: 1188 5482
PciI A'CATG_T
Cuts at: 5569 5569
Size: 6670
PflMI CCAn_nnn'nTGG
Cuts at: 1108 3313 1108
Size: 2205 4465
PfoI T'CCnGG_A
Cuts at: 3141 3141
Size: 6670
PmeI GTTT'AAAC
Cuts at: 3974 3974
Size: 6670
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4739 4771 6252 6284 4739
Size: 32 1481 32 5125
Fragments arranged by size:
5125 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6670
PsiI TTA'TAA
Cuts at: 4240 4240
Size: 6670
PspOMI G'GGCC_C
Cuts at: 2541 2885 2541
Size: 344 6326
PstI C_TGCA'G
Cuts at: 830 3174 3429 3702 3838 3870 4022 830
Size: 2344 255 273 136 32 152 3478
Fragments arranged by size:
3478 2344 273 255 152 136 32
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6670
PvuII CAG'CTG
Cuts at: 2143 3529 2143
Size: 1386 5284
SacI G_AGCT'C
Cuts at: 3456 3894 3456
Size: 438 6232
SalI G'TCGA_C
Cuts at: 1936 4012 1936
Size: 2076 4594
SapI GCTCTTCn'nnn_
Cuts at: 2286 2286
Size: 6670
SbfI CC_TGCA'GG
Cuts at: 4022 4022
Size: 6670
ScaI AGT'ACT
Cuts at: 4918 6630 4918
Size: 1712 4958
SexAI A'CCwGG_T
Cuts at: 3818 3818
Size: 6670
SfcI C'TryA_G
Cuts at: 826 1045 3170 3425 3698 3834 3866 4018 5153
Size: 219 2125 255 273 136 32 152 1135
Cuts at: 5153 5834 6025 826
Size: 681 191 1471
Fragments arranged by size:
2125 1471 1135 681 273 255 219 191
152 136 32
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6670
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6670
SmaI CCC'GGG
Cuts at: 1872 3997 1872
Size: 2125 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2411 2662 3751 3760 4774 5675
Size: 197 926 468 251 1089 9 1014 901
Cuts at: 5675 5937 820
Size: 262 1553
Fragments arranged by size:
1553 1089 1014 926 901 468 262 251
197 9
SnaBI TAC'GTA
Cuts at: 493 4461 493
Size: 3968 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6670
SphI G_CATG'C
Cuts at: 1132 2512 4028 1132
Size: 1380 1516 3774
Fragments arranged by size:
3774 1516 1380
SspI AAT'ATT
Cuts at: 5 52 4594 5
Size: 47 4542 2081
Fragments arranged by size:
4542 2081 47
StuI AGG'CCT
Cuts at: 1517 3923 1517
Size: 2406 4264
StyI C'CwwG_G
Cuts at: 513 1065 2202 2518 2881 3189 3735 3918 3924
Size: 552 1137 316 363 308 546 183 6
Cuts at: 3924 4109 513
Size: 185 3074
Fragments arranged by size:
3074 1137 552 546 363 316 308 185
183 6
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2444 2492 2963 4697 4714 4867 5052
Size: 1255 864 48 471 1734 17 153 185
Cuts at: 5052 5497 325
Size: 445 1498
Fragments arranged by size:
1734 1498 1255 864 471 445 185 153
48 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4916 6628 96
Size: 274 80 33 51 1443 2939 1712 138
Fragments arranged by size:
2939 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2937 2969 6630 6662 2937
Size: 32 3661 32 2945
Fragments arranged by size:
3661 2945 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6670
XbaI T'CTAG_A
Cuts at: 4006 4006
Size: 6670
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3196 3925 3196
Size: 729 5941
XhoI C'TCGA_G
Cuts at: 1943 3751 1943
Size: 1808 4862
XmaI C'CCGG_G
Cuts at: 1870 3995 1870
Size: 2125 4545
XmnI GAAnn'nnTTC
Cuts at: 3690 4799 3690
Size: 1109 5561
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4478 276
Size: 53 83 186 936 2944 2468
Fragments arranged by size:
2944 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI
BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI
BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I
EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI
KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI
NdeI NheI NotI NsiI NspI PasI PciI PflMI
PfoI PmeI PpiI PshAI PsiI PspOMI PstI PvuI
PvuII SacI SalI SapI SbfI ScaI SexAI SfcI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XcmI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI AscI AvrII BaeI BbeI BplI
BsiWI BsmBI BstZ17I Bsu36I EcoRV FseI FspAI KasI
NaeI NarI NgoMIV NruI PacI PmlI PpuMI PspXI
PsrI RsrII SacII SanDI SfiI SfoI SrfI SwaI