Restriction map for
pFN21AB5444
(Circular) (Six-base) MAPSORT of: pFN21AB5444.seq Check: 3559 from: 1 to: 6877
pFN21AB5444 6877 bp
With 182 enzymes: SgfI *
February 27, 2009 14:35 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 3472 3472
Size: 6877
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4687 278
Size: 53 83 186 936 3151 2468
Fragments arranged by size:
3151 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2265 4220 1937
Size: 328 1955 4594
Fragments arranged by size:
4594 1955 328
Acc65I G'GTAC_C
Cuts at: 2537 4198 2537
Size: 1661 5216
AclI AA'CG_TT
Cuts at: 1563 5004 5377 1563
Size: 3441 373 3063
Fragments arranged by size:
3441 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2339 4940 6324 6592 1632
Size: 707 2601 1384 268 1917
Fragments arranged by size:
2601 1917 1384 707 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6680
AflIII A'CryG_T
Cuts at: 1203 5776 6753 1203
Size: 4573 977 1327
Fragments arranged by size:
4573 1327 977
AgeI A'CCGG_T
Cuts at: 5708 5708
Size: 6877
AhdI GACnn_n'nnGTC
Cuts at: 5606 5606
Size: 6877
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6877
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6877
AlwNI CAG_nnn'CTG
Cuts at: 4118 4620 6192 4118
Size: 502 1572 4803
Fragments arranged by size:
4803 1572 502
ApaLI G'TGCA_C
Cuts at: 2018 4934 6090 2018
Size: 2916 1156 2805
Fragments arranged by size:
2916 2805 1156
ApoI r'AATT_y
Cuts at: 1493 2912 2987 3287 3584 4186 4412 4564 6673
Size: 1419 75 300 297 602 226 152 2109
Cuts at: 6673 1493
Size: 1697
Fragments arranged by size:
2109 1697 1419 602 300 297 226 152
75
AseI AT'TA_AT
Cuts at: 160 1032 5431 160
Size: 872 4399 1606
Fragments arranged by size:
4399 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6877
AvaI C'yCGr_G
Cuts at: 1870 1943 3177 4136 4202 1870
Size: 73 1234 959 66 4545
Fragments arranged by size:
4545 1234 959 73 66
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 2529 2562 3104 3137 2529
Size: 33 542 33 6269
Fragments arranged by size:
6269 542 33 33
BamHI G'GATC_C
Cuts at: 4018 4207 4574 4018
Size: 189 367 6321
Fragments arranged by size:
6321 367 189
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2537 4198 5653 618
Size: 325 217 627 750 1661 1455 1842
Fragments arranged by size:
1842 1661 1455 750 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2526 3022 3249 3552 4196 1391
Size: 1135 496 227 303 644 4072
Fragments arranged by size:
4072 1135 644 496 303 227
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2472 2949 3273 3339 3753 928
Size: 971 573 477 324 66 414 4052
Fragments arranged by size:
4052 971 573 477 414 324 66
BbvCI CC'TCA_GC
Cuts at: 3930 3930
Size: 6877
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 4133 4167 5068 5102 1903
Size: 34 50 34 2112 34 901 34 3678
Fragments arranged by size:
3678 2112 901 50 34 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4769 5979 4769
Size: 1210 5667
BclI T'GATC_A
Cuts at: 1548 2439 2836 4053 1548
Size: 891 397 1217 4372
Fragments arranged by size:
4372 1217 891 397
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2057 5488 136
Size: 107 122 71 631 605 385 3431 1525
Fragments arranged by size:
3431 1525 631 605 385 122 107 71
BglII A'GATC_T
Cuts at: 6872 6872
Size: 6877
BlpI GC'TnA_GC
Cuts at: 4294 4294
Size: 6877
Bme1580I G_kGCm'C
Cuts at: 1790 2022 4938 6094 1790
Size: 232 2916 1156 2573
Fragments arranged by size:
2916 2573 1156 232
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6877
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2947 3379 4608
Size: 416 53 83 399 24 1530 432 1229
Cuts at: 4608 5566 442
Size: 958 1753
Fragments arranged by size:
1753 1530 1229 958 432 416 399 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6877
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2152 3203 3268 3319 2152
Size: 1051 65 51 5710
Fragments arranged by size:
5710 1051 65 51
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2508 3173 3411 3647 3902 5537 1239
Size: 1269 665 238 236 255 1635 2579
Fragments arranged by size:
2579 1635 1269 665 255 238 236
Bpu10I CC'TnA_GC
Cuts at: 3930 3930
Size: 6877
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3263 3299 3563 4034 5002 5867 6165 3263
Size: 36 264 471 968 865 298 3975
Fragments arranged by size:
3975 968 865 471 298 264 36
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3610 5540 882
Size: 617 2111 1930 2219
Fragments arranged by size:
2219 2111 1930 617
BsaAI yAC'GTr
Cuts at: 493 1204 4668 493
Size: 711 3464 2702
Fragments arranged by size:
3464 2702 711
BsaBI GATnn'nnATC
Cuts at: 6871 6871
Size: 6877
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4684 5066 5701 275
Size: 53 83 186 936 3151 382 635 1451
Fragments arranged by size:
3151 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 5310 5708 5982 6129 6540 5310
Size: 398 274 147 411 5647
Fragments arranged by size:
5647 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 3320 3350 1178
Size: 30 165 30 1917 30 4705
Fragments arranged by size:
4705 1917 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2045 2048 2789 2915 3659 3662 1186
Size: 859 3 741 126 744 3 4401
Fragments arranged by size:
4401 859 744 741 126 3 3
BseYI C'CCAG_C
Cuts at: 6080 6576 6080
Size: 496 6381
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2385 3590 1163
Size: 573 649 1205 4450
Fragments arranged by size:
4450 1205 649 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4327 5088 6116 1540
Size: 275 186 2326 761 1028 2301
Fragments arranged by size:
2326 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2022 3249 4938 5023 6094 2022
Size: 1227 1689 85 1071 2805
Fragments arranged by size:
2805 1689 1227 1071 85
BsmI GAATG_Cn'
Cuts at: 2339 2617 3299 4388 4481 2339
Size: 278 682 1089 93 4735
Fragments arranged by size:
4735 1089 682 278 93
BsmBI CGTCTCn'nnnn_
Cuts at: 3905 4161 3905
Size: 256 6621
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2022 2526 3022 3249 3552 4196 4938
Size: 399 232 504 496 227 303 644 742
Cuts at: 4938 5023 6094 1391
Size: 85 1071 2174
Fragments arranged by size:
2174 1071 742 644 504 496 399 303
232 227 85
BspEI T'CCGG_A
Cuts at: 6540 6540
Size: 6877
BspHI T'CATG_A
Cuts at: 3702 3819 4766 6496 3702
Size: 117 947 1730 4083
Fragments arranged by size:
4083 1730 947 117
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3472 4232 844
Size: 2628 760 3489
Fragments arranged by size:
3489 2628 760
BsrBI CCG'CTC
Cuts at: 1394 1823 3814 4764 1394
Size: 429 1991 950 3507
Fragments arranged by size:
3507 1991 950 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2316 2996 5372 5546 65
Size: 1183 1068 680 2376 174 1396
Fragments arranged by size:
2376 1396 1183 1068 680 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 5521 5708 1700
Size: 110 3711 187 2869
Fragments arranged by size:
3711 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6877
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6877
BssSI C'ACGA_G
Cuts at: 4937 5949 4937
Size: 1012 5865
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2066 794
Size: 1272 5605
BstBI TT'CG_AA
Cuts at: 2192 5681 2192
Size: 3489 3388
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6877
BstYI r'GATC_y
Cuts at: 1972 3157 4018 4125 4207 4574 4959 4976 6428
Size: 1185 861 107 82 367 385 17 1452
Cuts at: 6428 6514 6526 6872 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1452 1185 861 385 367 346 107
86 82 17 12
Bsu36I CC'TnA_GG
Cuts at: 2010 2241 3636 2010
Size: 231 1395 5251
Fragments arranged by size:
5251 1395 231
BtgI C'CryG_G
Cuts at: 513 1065 2023 2515 3136 3852 513
Size: 552 958 492 621 716 3538
Fragments arranged by size:
3538 958 716 621 552 492
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 4158 487
Size: 683 56 2932 3206
Fragments arranged by size:
3206 2932 683 56
BtsI GCAGTG_nn'
Cuts at: 4393 5187 5207 4393
Size: 794 20 6063
Fragments arranged by size:
6063 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6877
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6842
DraI TTT'AAA
Cuts at: 4181 4181
Size: 6877
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 3409 3445 5884 809
Size: 507 2093 36 2439 1802
Fragments arranged by size:
2439 2093 1802 507 36
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3486 3867 4324
Size: 54 1425 50 275 18 1656 381 457
Cuts at: 4324 5213 6842 8
Size: 889 1629 43
Fragments arranged by size:
1656 1629 1425 889 457 381 275 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 4324 1537
Size: 275 2512 4090
Fragments arranged by size:
4090 2512 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2358 2580 2694 2712 3254 3590 3660 4807
Size: 1005 222 114 18 542 336 70 1147
Cuts at: 4807 1353
Size: 3423
Fragments arranged by size:
3423 1147 1005 542 336 222 114 70
18
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3065 5464 5838 5984 387
Size: 1064 138 1476 2399 374 146 1280
Fragments arranged by size:
2399 1476 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 3247 3247
Size: 6877
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2339 2508 3173 3411 3647 3902 4940
Size: 393 707 169 665 238 236 255 1038
Cuts at: 4940 5537 6324 6592 1239
Size: 597 787 268 1524
Fragments arranged by size:
1524 1038 787 707 665 597 393 268
255 238 236 169
EcoO109I rG'GnC_Cy
Cuts at: 3961 3961
Size: 6877
EcoRI G'AATT_C
Cuts at: 2912 2987 4186 4564 2912
Size: 75 1199 378 5225
Fragments arranged by size:
5225 1199 378 75
FspI TGC'GCA
Cuts at: 5383 5383
Size: 6877
HaeII r_GCGC'y
Cuts at: 1678 6024 1678
Size: 4346 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2539 2571 3389 3391 3421
Size: 32 135 32 967 32 818 2 30
Cuts at: 3421 3423 4117 4139 4149 4171 5518 5550 5592
Size: 2 694 22 10 22 1347 32 42
Cuts at: 5592 5624 1373
Size: 32 2626
Fragments arranged by size:
2626 1347 967 818 694 135 42 32
32 32 32 32 30 22 22 10
2 2
HincII GTy'rAC
Cuts at: 669 1657 1938 3148 4221 4467 669
Size: 988 281 1210 1073 246 3079
Fragments arranged by size:
3079 1210 1073 988 281 246
HindIII A'AGCT_T
Cuts at: 748 3317 748
Size: 2569 4308
HpaI GTT'AAC
Cuts at: 4467 4467
Size: 6877
KpnI G_GTAC'C
Cuts at: 2541 4202 2541
Size: 1661 5216
MfeI C'AATT_G
Cuts at: 4476 4476
Size: 6877
MluI A'CGCG_T
Cuts at: 6753 6753
Size: 6877
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2130 2178 2964 4059 5991 6175 1305
Size: 825 48 786 1095 1932 184 2007
Fragments arranged by size:
2007 1932 1095 825 786 184 48
MscI TGG'CCA
Cuts at: 10 64 1489 1832 3488 3869 10
Size: 54 1425 343 1656 381 3018
Fragments arranged by size:
3018 1656 1425 381 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2279 2375 2454 4835 5194 5353 518
Size: 648 1113 96 79 2381 359 159 2042
Fragments arranged by size:
2381 2042 1113 648 359 159 96 79
MspA1I CmG'CkG
Cuts at: 1606 2787 3099 4293 4970 6118 6363 1606
Size: 1181 312 1194 677 1148 245 2120
Fragments arranged by size:
2120 1194 1181 1148 677 312 245
NcoI C'CATG_G
Cuts at: 513 1065 2023 2515 3852 513
Size: 552 958 492 1337 3538
Fragments arranged by size:
3538 1337 958 552 492
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6877
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6877
NotI GC'GGCC_GC
Cuts at: 4324 4324
Size: 6877
NspI r_CATG'y
Cuts at: 1132 1229 4235 5780 1132
Size: 97 3006 1545 2229
Fragments arranged by size:
3006 2229 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6877
PciI A'CATG_T
Cuts at: 5776 5776
Size: 6877
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6877
PmeI GTTT'AAAC
Cuts at: 4181 4181
Size: 6877
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4946 4978 6459 6491 4946
Size: 32 1481 32 5332
Fragments arranged by size:
5332 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 3961 3961
Size: 6877
PshAI GACnn'nnGTC
Cuts at: 1613 3757 1613
Size: 2144 4733
PsiI TTA'TAA
Cuts at: 4447 4447
Size: 6877
PstI C_TGCA'G
Cuts at: 830 4114 4229 830
Size: 3284 115 3478
Fragments arranged by size:
3478 3284 115
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6877
PvuII CAG'CTG
Cuts at: 3099 3099
Size: 6877
SacI G_AGCT'C
Cuts at: 3249 3249
Size: 6877
SalI G'TCGA_C
Cuts at: 1936 4219 1936
Size: 2283 4594
SapI GCTCTTCn'nnn_
Cuts at: 2358 3254 3590 2358
Size: 896 336 5645
Fragments arranged by size:
5645 896 336
SbfI CC_TGCA'GG
Cuts at: 4229 4229
Size: 6877
ScaI AGT'ACT
Cuts at: 3563 5125 6837 3563
Size: 1562 1712 3603
Fragments arranged by size:
3603 1712 1562
SexAI A'CCwGG_T
Cuts at: 3963 3963
Size: 6877
SfcI C'TryA_G
Cuts at: 826 1045 2970 4110 4225 5360 6041 6232 826
Size: 219 1925 1140 115 1135 681 191 1471
Fragments arranged by size:
1925 1471 1140 1135 681 219 191 115
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6877
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6877
SmaI CCC'GGG
Cuts at: 1872 4204 1872
Size: 2332 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3242 3314 3578 4013 4136 4981
Size: 197 926 1299 72 264 435 123 845
Cuts at: 4981 5882 6144 820
Size: 901 262 1553
Fragments arranged by size:
1553 1299 926 901 845 435 264 262
197 123 72
SnaBI TAC'GTA
Cuts at: 493 4668 493
Size: 4175 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6877
SphI G_CATG'C
Cuts at: 1132 4235 1132
Size: 3103 3774
SspI AAT'ATT
Cuts at: 5 52 4801 5
Size: 47 4749 2081
Fragments arranged by size:
4749 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6877
StyI C'CwwG_G
Cuts at: 513 1065 2023 2154 2515 3615 3852 4316 513
Size: 552 958 131 361 1100 237 464 3074
Fragments arranged by size:
3074 1100 958 552 464 361 237 131
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2909 3560 4904 4921 5074 5259 5704
Size: 1255 1329 651 1344 17 153 185 445
Cuts at: 5704 325
Size: 1498
Fragments arranged by size:
1498 1344 1329 1255 651 445 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3561 5123 6835
Size: 274 80 33 51 1443 1584 1562 1712
Cuts at: 6835 96
Size: 138
Fragments arranged by size:
1712 1584 1562 1443 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3320 3352 6837 6869 3320
Size: 32 3485 32 3328
Fragments arranged by size:
3485 3328 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6877
XbaI T'CTAG_A
Cuts at: 4213 4213
Size: 6877
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2903 3622 3877 2903
Size: 719 255 5903
Fragments arranged by size:
5903 719 255
XhoI C'TCGA_G
Cuts at: 1943 4136 1943
Size: 2193 4684
XmaI C'CCGG_G
Cuts at: 1870 4202 1870
Size: 2332 4545
XmnI GAAnn'nnTTC
Cuts at: 5006 5006
Size: 6877
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4685 276
Size: 53 83 186 936 3151 2468
Fragments arranged by size:
3151 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BaeI BamHI BanI BanII BbsI BbvCI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII
BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I
EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI
MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI
NheI NotI NspI PasI PciI PflMI PmeI PpiI
PpuMI PshAI PsiI PstI PvuI PvuII SacI SalI
SapI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AfeI AloI ApaI AscI AvrII BbeI BfrBI BsiWI
BstXI BstZ17I DraIII EcoNI EcoRV FalI FseI FspAI
KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI
PmlI PspOMI PspXI PsrI RsrII SacII SanDI SfiI
SfoI SrfI SwaI