Restriction map for
pFN21AB5441
(Circular) (Six-base) MAPSORT of: pFN21AB5441.seq Check: 1935 from: 1 to: 5722
pFN21AB5441 5722 bp
With 182 enzymes: SgfI *
October 20, 2009 10:40 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3532 278
Size: 53 83 186 936 1996 2468
Fragments arranged by size:
2468 1996 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3065 1937
Size: 1128 4594
Acc65I G'GTAC_C
Cuts at: 2183 3043 2183
Size: 860 4862
AclI AA'CG_TT
Cuts at: 1563 3849 4222 1563
Size: 2286 373 3063
Fragments arranged by size:
3063 2286 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2258 2493 3785 5169 5437 1632
Size: 626 235 1292 1384 268 1917
Fragments arranged by size:
1917 1384 1292 626 268 235
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5525
AflIII A'CryG_T
Cuts at: 1203 2809 4621 5598 1203
Size: 1606 1812 977 1327
Fragments arranged by size:
1812 1606 1327 977
AgeI A'CCGG_T
Cuts at: 4553 4553
Size: 5722
AhdI GACnn_n'nnGTC
Cuts at: 4451 4451
Size: 5722
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5722
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5722
AlwNI CAG_nnn'CTG
Cuts at: 3465 5037 3465
Size: 1572 4150
ApaI G_GGCC'C
Cuts at: 2202 2202
Size: 5722
ApaLI G'TGCA_C
Cuts at: 3779 4935 3779
Size: 1156 4566
ApoI r'AATT_y
Cuts at: 1493 2988 3031 3257 3409 5518 1493
Size: 1495 43 226 152 2109 1697
Fragments arranged by size:
2109 1697 1495 226 152 43
AseI AT'TA_AT
Cuts at: 160 1032 4276 160
Size: 872 3244 1606
Fragments arranged by size:
3244 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5722
AvaI C'yCGr_G
Cuts at: 1870 1943 3047 1870
Size: 73 1104 4545
Fragments arranged by size:
4545 1104 73
BamHI G'GATC_C
Cuts at: 3052 3419 3052
Size: 367 5355
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2183 3043 4498 618
Size: 325 217 627 396 860 1455 1842
Fragments arranged by size:
1842 1455 860 627 396 325 217
BanII G_rGCy'C
Cuts at: 1391 2202 2315 3041 1391
Size: 811 113 726 4072
Fragments arranged by size:
4072 811 726 113
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4751
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3913 3947 1903
Size: 34 1976 34 3678
Fragments arranged by size:
3678 1976 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2675 3614 4824 2675
Size: 939 1210 3573
Fragments arranged by size:
3573 1210 939
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5722
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2339 4333 136
Size: 107 122 71 631 605 667 1994 1525
Fragments arranged by size:
1994 1525 667 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2631 2730 5717 2631
Size: 99 2987 2636
Fragments arranged by size:
2987 2636 99
BlpI GC'TnA_GC
Cuts at: 3139 3139
Size: 5722
Bme1580I G_kGCm'C
Cuts at: 1790 2139 2202 3783 4939 1790
Size: 349 63 1581 1156 2573
Fragments arranged by size:
2573 1581 1156 349 63
BmgBI CAC'GTC
Cuts at: 1318 2389 2575 2812 1318
Size: 1071 186 237 4228
Fragments arranged by size:
4228 1071 237 186
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2923 3019 3453
Size: 416 53 83 399 24 1506 96 434
Cuts at: 3453 4411 442
Size: 958 1753
Fragments arranged by size:
1753 1506 958 434 416 399 96 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5722
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 4382 1239
Size: 3143 2579
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3847 4712 5010 3847
Size: 865 298 4559
Fragments arranged by size:
4559 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2298 4385 882
Size: 617 799 2087 2219
Fragments arranged by size:
2219 2087 799 617
BsaAI yAC'GTr
Cuts at: 493 1204 2124 3513 493
Size: 711 920 1389 2702
Fragments arranged by size:
2702 1389 920 711
BsaBI GATnn'nnATC
Cuts at: 2369 5716 2369
Size: 3347 2375
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3529 3911 4546 275
Size: 53 83 186 936 1996 382 635 1451
Fragments arranged by size:
1996 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 2361 2816 4155 4553 4827 4974 5385 2361
Size: 455 1339 398 274 147 411 2698
Fragments arranged by size:
2698 1339 455 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2297 2327 2684 2714 1178
Size: 30 165 30 894 30 357 30 4186
Fragments arranged by size:
4186 894 357 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 5722
BseYI C'CCAG_C
Cuts at: 2328 2856 4925 5421 2328
Size: 528 2069 496 2629
Fragments arranged by size:
2629 2069 528 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2560 1163
Size: 573 824 4325
Fragments arranged by size:
4325 824 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3172 3933 4961 1540
Size: 275 186 1171 761 1028 2301
Fragments arranged by size:
2301 1171 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2571 3783 3868 4939 2571
Size: 1212 85 1071 3354
Fragments arranged by size:
3354 1212 1071 85
BsmI GAATG_Cn'
Cuts at: 3233 3326 3233
Size: 93 5629
BsmBI CGTCTCn'nnnn_
Cuts at: 2704 2704
Size: 5722
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2139 2202 2315 2571 3041 3783 3868
Size: 399 349 63 113 256 470 742 85
Cuts at: 3868 4939 1391
Size: 1071 2174
Fragments arranged by size:
2174 1071 742 470 399 349 256 113
85 63
BspEI T'CCGG_A
Cuts at: 5385 5385
Size: 5722
BspHI T'CATG_A
Cuts at: 3611 5341 3611
Size: 1730 3992
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2424 3077 844
Size: 1580 653 3489
Fragments arranged by size:
3489 1580 653
BsrBI CCG'CTC
Cuts at: 1394 1823 3609 1394
Size: 429 1786 3507
Fragments arranged by size:
3507 1786 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4217 4391 65
Size: 1183 2969 174 1396
Fragments arranged by size:
2969 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4366 4553 1700
Size: 110 2556 187 2869
Fragments arranged by size:
2869 2556 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5722
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5722
BssSI C'ACGA_G
Cuts at: 3782 4794 3782
Size: 1012 4710
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5722
BstBI TT'CG_AA
Cuts at: 4526 4526
Size: 5722
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5722
BstYI r'GATC_y
Cuts at: 1972 2364 2631 2730 3052 3419 3804 3821 5273
Size: 392 267 99 322 367 385 17 1452
Cuts at: 5273 5359 5371 5717 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1452 392 385 367 346 322 267
99 86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2748 513
Size: 552 1683 3487
Fragments arranged by size:
3487 1683 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4983
Fragments arranged by size:
4983 683 56
BtsI GCAGTG_nn'
Cuts at: 3238 4032 4052 3238
Size: 794 20 4908
Fragments arranged by size:
4908 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5722
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5687
DraI TTT'AAA
Cuts at: 2935 3026 2935
Size: 91 5631
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4729 809
Size: 507 3413 1802
Fragments arranged by size:
3413 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2046 2273 2724
Size: 54 1425 50 275 18 216 227 451
Cuts at: 2724 2833 3169 4058 5687 8
Size: 109 336 889 1629 43
Fragments arranged by size:
1629 1425 889 451 336 275 227 216
109 54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3169 1537
Size: 275 1357 4090
Fragments arranged by size:
4090 1357 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2165 3652 1353
Size: 812 1487 3423
Fragments arranged by size:
3423 1487 812
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2423 4309 4683 4829 387
Size: 1064 138 834 1886 374 146 1280
Fragments arranged by size:
1886 1280 1064 834 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2258 2493 3785 4382 5169 5437 1239
Size: 393 626 235 1292 597 787 268 1524
Fragments arranged by size:
1524 1292 787 626 597 393 268 235
EcoO109I rG'GnC_Cy
Cuts at: 2064 2233 2413 2064
Size: 169 180 5373
Fragments arranged by size:
5373 180 169
EcoRI G'AATT_C
Cuts at: 2988 3031 3409 2988
Size: 43 378 5301
Fragments arranged by size:
5301 378 43
FspI TGC'GCA
Cuts at: 2385 4228 2385
Size: 1843 3879
FspAI rTGC'GCAy
Cuts at: 2385 2385
Size: 5722
HaeII r_GCGC'y
Cuts at: 1678 4869 1678
Size: 3191 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2297 2329 4363 4395 4437
Size: 32 135 32 725 32 2034 32 42
Cuts at: 4437 4469 1373
Size: 32 2626
Fragments arranged by size:
2626 2034 725 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2098 2107 2578 3066 3312 669
Size: 988 281 160 9 471 488 246 3079
Fragments arranged by size:
3079 988 488 471 281 246 160 9
HindIII A'AGCT_T
Cuts at: 748 2251 2475 748
Size: 1503 224 3995
Fragments arranged by size:
3995 1503 224
HpaI GTT'AAC
Cuts at: 3312 3312
Size: 5722
KpnI G_GTAC'C
Cuts at: 2187 3047 2187
Size: 860 4862
MfeI C'AATT_G
Cuts at: 3321 3321
Size: 5722
MluI A'CGCG_T
Cuts at: 5598 5598
Size: 5722
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2064 4836 5020 1305
Size: 759 2772 184 2007
Fragments arranged by size:
2772 2007 759 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2048 2275 2835 10
Size: 54 1425 343 216 227 560 2897
Fragments arranged by size:
2897 1425 560 343 227 216 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2379 3680 4039 4198 518
Size: 648 1213 1301 359 159 2042
Fragments arranged by size:
2042 1301 1213 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 3138 3815 4963 5208 1606
Size: 1532 677 1148 245 2120
Fragments arranged by size:
2120 1532 1148 677 245
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5170
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5722
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5722
NotI GC'GGCC_GC
Cuts at: 3169 3169
Size: 5722
NspI r_CATG'y
Cuts at: 1132 1229 2427 2440 3080 4625 1132
Size: 97 1198 13 640 1545 2229
Fragments arranged by size:
2229 1545 1198 640 97 13
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5722
PciI A'CATG_T
Cuts at: 4621 4621
Size: 5722
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2901 1108
Size: 1793 3929
PfoI T'CCnGG_A
Cuts at: 2900 2900
Size: 5722
PmeI GTTT'AAAC
Cuts at: 3026 3026
Size: 5722
PmlI CAC'GTG
Cuts at: 2124 2124
Size: 5722
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3791 3823 5304 5336 3791
Size: 32 1481 32 4177
Fragments arranged by size:
4177 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2233 2413 2233
Size: 180 5542
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5722
PsiI TTA'TAA
Cuts at: 3292 3292
Size: 5722
PspOMI G'GGCC_C
Cuts at: 2198 2198
Size: 5722
PstI C_TGCA'G
Cuts at: 830 3074 830
Size: 2244 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5722
SalI G'TCGA_C
Cuts at: 1936 3064 1936
Size: 1128 4594
SbfI CC_TGCA'GG
Cuts at: 3074 3074
Size: 5722
ScaI AGT'ACT
Cuts at: 3008 3970 5682 3008
Size: 962 1712 3048
Fragments arranged by size:
3048 1712 962
SfcI C'TryA_G
Cuts at: 826 1045 2647 2692 3070 4205 4886 5077 826
Size: 219 1602 45 378 1135 681 191 1471
Fragments arranged by size:
1602 1471 1135 681 378 219 191 45
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5722
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5722
SmaI CCC'GGG
Cuts at: 1872 3049 1872
Size: 1177 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3826 4727 4989 820
Size: 197 926 1883 901 262 1553
Fragments arranged by size:
1883 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3513 493
Size: 3020 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5722
SphI G_CATG'C
Cuts at: 1132 3080 1132
Size: 1948 3774
SspI AAT'ATT
Cuts at: 5 52 3646 5
Size: 47 3594 2081
Fragments arranged by size:
3594 2081 47
StuI AGG'CCT
Cuts at: 1517 2502 1517
Size: 985 4737
StyI C'CwwG_G
Cuts at: 513 1065 3161 513
Size: 552 2096 3074
Fragments arranged by size:
3074 2096 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2183 2819 3749 3766 3919 4104 4549
Size: 1255 603 636 930 17 153 185 445
Cuts at: 4549 325
Size: 1498
Fragments arranged by size:
1498 1255 930 636 603 445 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3006 3968 5680
Size: 274 80 33 51 1443 1029 962 1712
Cuts at: 5680 96
Size: 138
Fragments arranged by size:
1712 1443 1029 962 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5682 5714 5682
Size: 32 5690
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5722
XbaI T'CTAG_A
Cuts at: 3058 3058
Size: 5722
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2315 2848 2315
Size: 533 5189
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5722
XmaI C'CCGG_G
Cuts at: 1870 3047 1870
Size: 1177 4545
XmnI GAAnn'nnTTC
Cuts at: 3851 3851
Size: 5722
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3530 276
Size: 53 83 186 936 1996 2468
Fragments arranged by size:
2468 1996 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI
ClaI CspCI DraI DrdI EaeI EagI EarI EciI
Eco57MI EcoO109I EcoRI FspI FspAI HaeII Hin4I HincII
HindIII HpaI KpnI MfeI MluI MmeI MscI MslI
MspA1I NcoI NdeI NheI NotI NspI PasI PciI
PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI
PspOMI PstI PvuI SalI SbfI ScaI SfcI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI AscI AvrII BaeI BbeI BbvCI
BfrBI BplI Bpu10I BsiWI BstXI BstZ17I Bsu36I DraIII
EcoICRI EcoNI EcoRV FalI FseI KasI NaeI NarI
NgoMIV NruI NsiI PacI PspXI PsrI PvuII RsrII
SacI SacII SanDI SapI SexAI SfiI SfoI SrfI
SwaI