(Circular) (Six-base) MAPSORT of: pFN21AB5440.seq Check: 2533 from: 1 to: 5686 pFN21AB5440 5686 bp With 182 enzymes: SgfI * April 15, 2010 09:31 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2784 2784 Size: 5686 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3496 278 Size: 53 83 186 936 1960 2468 Fragments arranged by size: 2468 1960 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3029 1937 Size: 1092 4594 Acc65I G'GTAC_C Cuts at: 3007 3007 Size: 5686 AclI AA'CG_TT Cuts at: 1563 3813 4186 1563 Size: 2250 373 3063 Fragments arranged by size: 3063 2250 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2398 3749 5133 5401 1632 Size: 766 1351 1384 268 1917 Fragments arranged by size: 1917 1384 1351 766 268 AfeI AGC'GCT Cuts at: 2433 2433 Size: 5686 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5489 AflIII A'CryG_T Cuts at: 1203 4585 5562 1203 Size: 3382 977 1327 Fragments arranged by size: 3382 1327 977 AgeI A'CCGG_T Cuts at: 4517 4517 Size: 5686 AhdI GACnn_n'nnGTC Cuts at: 2053 4415 2053 Size: 2362 3324 AleI CACnn'nnGTG Cuts at: 1166 2971 1166 Size: 1805 3881 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2633 1184 Size: 1449 4237 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2678 2710 2678 Size: 32 5654 AlwNI CAG_nnn'CTG Cuts at: 3429 5001 3429 Size: 1572 4114 ApaI G_GGCC'C Cuts at: 2216 2279 2550 2920 2216 Size: 63 271 370 4982 Fragments arranged by size: 4982 370 271 63 ApaLI G'TGCA_C Cuts at: 2974 3743 4899 2974 Size: 769 1156 3761 Fragments arranged by size: 3761 1156 769 ApoI r'AATT_y Cuts at: 1493 2995 3221 3373 5482 1493 Size: 1502 226 152 2109 1697 Fragments arranged by size: 2109 1697 1502 226 152 AseI AT'TA_AT Cuts at: 160 1032 4240 160 Size: 872 3208 1606 Fragments arranged by size: 3208 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5686 AvaI C'yCGr_G Cuts at: 1870 1943 2178 2209 2740 3011 1870 Size: 73 235 31 531 271 4545 Fragments arranged by size: 4545 531 271 235 73 31 BamHI G'GATC_C Cuts at: 3016 3383 3016 Size: 367 5319 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2299 2379 3007 4462 618 Size: 325 217 627 512 80 628 1455 1842 Fragments arranged by size: 1842 1455 628 627 512 325 217 80 BanII G_rGCy'C Cuts at: 1391 2216 2279 2328 2550 2574 2724 2920 3005 Size: 825 63 49 222 24 150 196 85 Cuts at: 3005 1391 Size: 4072 Fragments arranged by size: 4072 825 222 196 150 85 63 49 24 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2089 2268 2326 928 Size: 971 190 179 58 4288 Fragments arranged by size: 4288 971 190 179 58 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3877 3911 1903 Size: 34 1940 34 3678 Fragments arranged by size: 3678 1940 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3578 4788 3578 Size: 1210 4476 BclI T'GATC_A Cuts at: 1548 1548 Size: 5686 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4297 136 Size: 107 122 71 631 605 2625 1525 Fragments arranged by size: 2625 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 5681 5681 Size: 5686 BlpI GC'TnA_GC Cuts at: 2156 3103 2156 Size: 947 4739 Bme1580I G_kGCm'C Cuts at: 1790 2216 2279 2304 2343 2550 2920 2978 3747 Size: 426 63 25 39 207 370 58 769 Cuts at: 3747 4903 1790 Size: 1156 2573 Fragments arranged by size: 2573 1156 769 426 370 207 63 58 39 25 BmgBI CAC'GTC Cuts at: 1318 2173 1318 Size: 855 4831 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2263 2763 3417 Size: 416 53 83 399 24 846 500 654 Cuts at: 3417 4375 442 Size: 958 1753 Fragments arranged by size: 1753 958 846 654 500 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5686 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2738 2738 Size: 5686 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2474 2708 4346 1239 Size: 1235 234 1638 2579 Fragments arranged by size: 2579 1638 1235 234 Bpu10I CC'TnA_GC Cuts at: 2247 2360 2247 Size: 113 5573 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3811 4676 4974 3811 Size: 865 298 4523 Fragments arranged by size: 4523 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2198 4349 882 Size: 617 699 2151 2219 Fragments arranged by size: 2219 2151 699 617 BsaAI yAC'GTr Cuts at: 493 1204 2392 2557 3477 493 Size: 711 1188 165 920 2702 Fragments arranged by size: 2702 1188 920 711 165 BsaBI GATnn'nnATC Cuts at: 5680 5680 Size: 5686 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3493 3875 4510 275 Size: 53 83 186 936 1960 382 635 1451 Fragments arranged by size: 1960 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4119 4517 4791 4938 5349 4119 Size: 398 274 147 411 4456 Fragments arranged by size: 4456 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2546 2576 1178 Size: 30 165 30 1143 30 4288 Fragments arranged by size: 4288 1143 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2336 2531 1186 Size: 1150 195 4341 Fragments arranged by size: 4341 1150 195 BseYI C'CCAG_C Cuts at: 2125 2543 2673 2717 2785 4889 5385 2125 Size: 418 130 44 68 2104 496 2426 Fragments arranged by size: 2426 2104 496 418 130 68 44 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2908 1163 Size: 573 1172 3941 Fragments arranged by size: 3941 1172 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3136 3897 4925 1540 Size: 275 186 1135 761 1028 2301 Fragments arranged by size: 2301 1135 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2253 2328 2397 2574 2978 3747 3832 4903 2253 Size: 75 69 177 404 769 85 1071 3036 Fragments arranged by size: 3036 1071 769 404 177 85 75 69 BsmI GAATG_Cn' Cuts at: 3197 3290 3197 Size: 93 5593 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2216 2253 2279 2304 2328 2343 2397 Size: 399 426 37 26 25 24 15 54 Cuts at: 2397 2550 2574 2724 2920 2978 3005 3747 3832 Size: 153 24 150 196 58 27 742 85 Cuts at: 3832 4903 1391 Size: 1071 2174 Fragments arranged by size: 2174 1071 742 426 399 196 153 150 85 58 54 37 27 26 25 24 24 15 BspEI T'CCGG_A Cuts at: 5349 5349 Size: 5686 BspHI T'CATG_A Cuts at: 3575 5305 3575 Size: 1730 3956 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2043 2508 2784 3041 844 Size: 1199 465 276 257 3489 Fragments arranged by size: 3489 1199 465 276 257 BsrBI CCG'CTC Cuts at: 1394 1823 3573 1394 Size: 429 1750 3507 Fragments arranged by size: 3507 1750 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2478 2653 4181 4355 65 Size: 1183 1230 175 1528 174 1396 Fragments arranged by size: 1528 1396 1230 1183 175 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2637 2700 4330 4517 1700 Size: 110 827 63 1630 187 2869 Fragments arranged by size: 2869 1630 827 187 110 63 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5686 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5686 BssSI C'ACGA_G Cuts at: 3746 4758 3746 Size: 1012 4674 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5686 BstBI TT'CG_AA Cuts at: 4490 4490 Size: 5686 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5686 BstYI r'GATC_y Cuts at: 1972 2373 3016 3383 3768 3785 5237 5323 5335 Size: 401 643 367 385 17 1452 86 12 Cuts at: 5335 5681 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 643 401 385 367 346 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2538 2538 Size: 5686 BtgI C'CryG_G Cuts at: 513 1065 513 Size: 552 5134 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2800 487 Size: 683 56 1574 3373 Fragments arranged by size: 3373 1574 683 56 BtsI GCAGTG_nn' Cuts at: 2298 2895 3202 3996 4016 2298 Size: 597 307 794 20 3968 Fragments arranged by size: 3968 794 597 307 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5686 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5651 DraI TTT'AAA Cuts at: 2990 2990 Size: 5686 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4693 809 Size: 507 3377 1802 Fragments arranged by size: 3377 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3133 4022 5651 Size: 54 1425 50 275 18 1303 889 1629 Cuts at: 5651 8 Size: 43 Fragments arranged by size: 1629 1425 1303 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3133 1537 Size: 275 1321 4090 Fragments arranged by size: 4090 1321 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2792 3616 1353 Size: 1439 824 3423 Fragments arranged by size: 3423 1439 824 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2723 2809 4273 4647 4793 387 Size: 1064 138 1134 86 1464 374 146 1280 Fragments arranged by size: 1464 1280 1134 1064 374 146 138 86 EcoICRI GAG'CTC Cuts at: 2326 2572 2326 Size: 246 5440 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2398 2474 2708 3749 4346 5133 5401 Size: 393 766 76 234 1041 597 787 268 Cuts at: 5401 1239 Size: 1524 Fragments arranged by size: 1524 1041 787 766 597 393 268 234 76 EcoNI CCTnn'n_nnAGG Cuts at: 2017 2017 Size: 5686 EcoO109I rG'GnC_Cy Cuts at: 2213 2275 2276 2547 2916 2917 2213 Size: 62 1 271 369 1 4982 Fragments arranged by size: 4982 369 271 62 1 1 EcoRI G'AATT_C Cuts at: 2995 3373 2995 Size: 378 5308 FspI TGC'GCA Cuts at: 4192 4192 Size: 5686 HaeII r_GCGC'y Cuts at: 1678 2435 4833 1678 Size: 757 2398 2531 Fragments arranged by size: 2531 2398 757 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2039 2071 4327 4359 4401 Size: 32 135 32 467 32 2256 32 42 Cuts at: 4401 4433 1373 Size: 32 2626 Fragments arranged by size: 2626 2256 467 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2497 3030 3276 669 Size: 988 281 559 533 246 3079 Fragments arranged by size: 3079 988 559 533 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5686 HpaI GTT'AAC Cuts at: 3276 3276 Size: 5686 KpnI G_GTAC'C Cuts at: 3011 3011 Size: 5686 MfeI C'AATT_G Cuts at: 3285 3285 Size: 5686 MluI A'CGCG_T Cuts at: 5562 5562 Size: 5686 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2682 2925 4800 4984 1305 Size: 1377 243 1875 184 2007 Fragments arranged by size: 2007 1875 1377 243 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 3864 Fragments arranged by size: 3864 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2390 2441 2483 2778 2971 3644 4003 Size: 648 1224 51 42 295 193 673 359 Cuts at: 4003 4162 518 Size: 159 2042 Fragments arranged by size: 2042 1224 673 648 359 295 193 159 51 42 MspA1I CmG'CkG Cuts at: 1606 2035 2160 3102 3779 4927 5172 1606 Size: 429 125 942 677 1148 245 2120 Fragments arranged by size: 2120 1148 942 677 429 245 125 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 5134 NdeI CA'TA_TG Cuts at: 387 387 Size: 5686 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5686 NotI GC'GGCC_GC Cuts at: 3133 3133 Size: 5686 NspI r_CATG'y Cuts at: 1132 1229 2350 3044 4589 1132 Size: 97 1121 694 1545 2229 Fragments arranged by size: 2229 1545 1121 694 97 PasI CC'CwG_GG Cuts at: 1348 2040 1348 Size: 692 4994 PciI A'CATG_T Cuts at: 4585 4585 Size: 5686 PflMI CCAn_nnn'nTGG Cuts at: 1108 2287 2970 1108 Size: 1179 683 3824 Fragments arranged by size: 3824 1179 683 PfoI T'CCnGG_A Cuts at: 2451 2451 Size: 5686 PmeI GTTT'AAAC Cuts at: 2990 2990 Size: 5686 PmlI CAC'GTG Cuts at: 2557 2557 Size: 5686 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3755 3787 5268 5300 3755 Size: 32 1481 32 4141 Fragments arranged by size: 4141 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 2600 1613 Size: 987 4699 PsiI TTA'TAA Cuts at: 3256 3256 Size: 5686 PspOMI G'GGCC_C Cuts at: 2212 2275 2546 2916 2212 Size: 63 271 370 4982 Fragments arranged by size: 4982 370 271 63 PstI C_TGCA'G Cuts at: 830 2154 3038 830 Size: 1324 884 3478 Fragments arranged by size: 3478 1324 884 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5686 PvuII CAG'CTG Cuts at: 2035 2160 2035 Size: 125 5561 SacI G_AGCT'C Cuts at: 2328 2574 2328 Size: 246 5440 SalI G'TCGA_C Cuts at: 1936 3028 1936 Size: 1092 4594 SbfI CC_TGCA'GG Cuts at: 3038 3038 Size: 5686 ScaI AGT'ACT Cuts at: 3934 5646 3934 Size: 1712 3974 SexAI A'CCwGG_T Cuts at: 2400 2400 Size: 5686 SfcI C'TryA_G Cuts at: 826 1045 2150 3034 4169 4850 5041 826 Size: 219 1105 884 1135 681 191 1471 Fragments arranged by size: 1471 1135 1105 884 681 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5686 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5686 SmaI CCC'GGG Cuts at: 1872 2180 3013 1872 Size: 308 833 4545 Fragments arranged by size: 4545 833 308 SmlI C'TyrA_G Cuts at: 820 1017 1943 3790 4691 4953 820 Size: 197 926 1847 901 262 1553 Fragments arranged by size: 1847 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3477 493 Size: 2984 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5686 SphI G_CATG'C Cuts at: 1132 3044 1132 Size: 1912 3774 SrfI GCCC'GGGC Cuts at: 2180 2180 Size: 5686 SspI AAT'ATT Cuts at: 5 52 3610 5 Size: 47 3558 2081 Fragments arranged by size: 3558 2081 47 StuI AGG'CCT Cuts at: 1517 2862 1517 Size: 1345 4341 StyI C'CwwG_G Cuts at: 513 1065 3125 513 Size: 552 2060 3074 Fragments arranged by size: 3074 2060 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3713 3730 3883 4068 4513 325 Size: 1255 2133 17 153 185 445 1498 Fragments arranged by size: 2133 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3932 5644 96 Size: 274 80 33 51 1443 1955 1712 138 Fragments arranged by size: 1955 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2546 2578 5646 5678 2546 Size: 32 3068 32 2554 Fragments arranged by size: 3068 2554 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5686 XbaI T'CTAG_A Cuts at: 3022 3022 Size: 5686 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5686 XmaI C'CCGG_G Cuts at: 1870 2178 3011 1870 Size: 308 833 4545 Fragments arranged by size: 4545 833 308 XmnI GAAnn'nnTTC Cuts at: 3815 3815 Size: 5686 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3494 276 Size: 53 83 186 936 1960 2468 Fragments arranged by size: 2468 1960 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PshAI PsiI PspOMI PstI PvuI PvuII SacI SalI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SrfI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AscI AvrII BaeI BbeI BbvCI BfrBI BsiWI BsmBI BstXI BstZ17I DraIII EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PpuMI PspXI PsrI RsrII SacII SanDI SapI SfiI SfoI SwaI XcmI