Restriction map for
pFN21AB5440
(Circular) (Six-base) MAPSORT of: pFN21AB5440.seq Check: 2533 from: 1 to: 5686
pFN21AB5440 5686 bp
With 182 enzymes: SgfI *
April 15, 2010 09:31 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2784 2784
Size: 5686
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3496 278
Size: 53 83 186 936 1960 2468
Fragments arranged by size:
2468 1960 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3029 1937
Size: 1092 4594
Acc65I G'GTAC_C
Cuts at: 3007 3007
Size: 5686
AclI AA'CG_TT
Cuts at: 1563 3813 4186 1563
Size: 2250 373 3063
Fragments arranged by size:
3063 2250 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2398 3749 5133 5401 1632
Size: 766 1351 1384 268 1917
Fragments arranged by size:
1917 1384 1351 766 268
AfeI AGC'GCT
Cuts at: 2433 2433
Size: 5686
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5489
AflIII A'CryG_T
Cuts at: 1203 4585 5562 1203
Size: 3382 977 1327
Fragments arranged by size:
3382 1327 977
AgeI A'CCGG_T
Cuts at: 4517 4517
Size: 5686
AhdI GACnn_n'nnGTC
Cuts at: 2053 4415 2053
Size: 2362 3324
AleI CACnn'nnGTG
Cuts at: 1166 2971 1166
Size: 1805 3881
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2633 1184
Size: 1449 4237
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2678 2710 2678
Size: 32 5654
AlwNI CAG_nnn'CTG
Cuts at: 3429 5001 3429
Size: 1572 4114
ApaI G_GGCC'C
Cuts at: 2216 2279 2550 2920 2216
Size: 63 271 370 4982
Fragments arranged by size:
4982 370 271 63
ApaLI G'TGCA_C
Cuts at: 2974 3743 4899 2974
Size: 769 1156 3761
Fragments arranged by size:
3761 1156 769
ApoI r'AATT_y
Cuts at: 1493 2995 3221 3373 5482 1493
Size: 1502 226 152 2109 1697
Fragments arranged by size:
2109 1697 1502 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4240 160
Size: 872 3208 1606
Fragments arranged by size:
3208 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5686
AvaI C'yCGr_G
Cuts at: 1870 1943 2178 2209 2740 3011 1870
Size: 73 235 31 531 271 4545
Fragments arranged by size:
4545 531 271 235 73 31
BamHI G'GATC_C
Cuts at: 3016 3383 3016
Size: 367 5319
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2299 2379 3007 4462 618
Size: 325 217 627 512 80 628 1455 1842
Fragments arranged by size:
1842 1455 628 627 512 325 217 80
BanII G_rGCy'C
Cuts at: 1391 2216 2279 2328 2550 2574 2724 2920 3005
Size: 825 63 49 222 24 150 196 85
Cuts at: 3005 1391
Size: 4072
Fragments arranged by size:
4072 825 222 196 150 85 63 49
24
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2089 2268 2326 928
Size: 971 190 179 58 4288
Fragments arranged by size:
4288 971 190 179 58
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3877 3911 1903
Size: 34 1940 34 3678
Fragments arranged by size:
3678 1940 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3578 4788 3578
Size: 1210 4476
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5686
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4297 136
Size: 107 122 71 631 605 2625 1525
Fragments arranged by size:
2625 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5681 5681
Size: 5686
BlpI GC'TnA_GC
Cuts at: 2156 3103 2156
Size: 947 4739
Bme1580I G_kGCm'C
Cuts at: 1790 2216 2279 2304 2343 2550 2920 2978 3747
Size: 426 63 25 39 207 370 58 769
Cuts at: 3747 4903 1790
Size: 1156 2573
Fragments arranged by size:
2573 1156 769 426 370 207 63 58
39 25
BmgBI CAC'GTC
Cuts at: 1318 2173 1318
Size: 855 4831
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2263 2763 3417
Size: 416 53 83 399 24 846 500 654
Cuts at: 3417 4375 442
Size: 958 1753
Fragments arranged by size:
1753 958 846 654 500 416 399 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5686
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2738 2738
Size: 5686
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2474 2708 4346 1239
Size: 1235 234 1638 2579
Fragments arranged by size:
2579 1638 1235 234
Bpu10I CC'TnA_GC
Cuts at: 2247 2360 2247
Size: 113 5573
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3811 4676 4974 3811
Size: 865 298 4523
Fragments arranged by size:
4523 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2198 4349 882
Size: 617 699 2151 2219
Fragments arranged by size:
2219 2151 699 617
BsaAI yAC'GTr
Cuts at: 493 1204 2392 2557 3477 493
Size: 711 1188 165 920 2702
Fragments arranged by size:
2702 1188 920 711 165
BsaBI GATnn'nnATC
Cuts at: 5680 5680
Size: 5686
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3493 3875 4510 275
Size: 53 83 186 936 1960 382 635 1451
Fragments arranged by size:
1960 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4119 4517 4791 4938 5349 4119
Size: 398 274 147 411 4456
Fragments arranged by size:
4456 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2546 2576 1178
Size: 30 165 30 1143 30 4288
Fragments arranged by size:
4288 1143 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2336 2531 1186
Size: 1150 195 4341
Fragments arranged by size:
4341 1150 195
BseYI C'CCAG_C
Cuts at: 2125 2543 2673 2717 2785 4889 5385 2125
Size: 418 130 44 68 2104 496 2426
Fragments arranged by size:
2426 2104 496 418 130 68 44
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2908 1163
Size: 573 1172 3941
Fragments arranged by size:
3941 1172 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3136 3897 4925 1540
Size: 275 186 1135 761 1028 2301
Fragments arranged by size:
2301 1135 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2253 2328 2397 2574 2978 3747 3832 4903 2253
Size: 75 69 177 404 769 85 1071 3036
Fragments arranged by size:
3036 1071 769 404 177 85 75 69
BsmI GAATG_Cn'
Cuts at: 3197 3290 3197
Size: 93 5593
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2216 2253 2279 2304 2328 2343 2397
Size: 399 426 37 26 25 24 15 54
Cuts at: 2397 2550 2574 2724 2920 2978 3005 3747 3832
Size: 153 24 150 196 58 27 742 85
Cuts at: 3832 4903 1391
Size: 1071 2174
Fragments arranged by size:
2174 1071 742 426 399 196 153 150
85 58 54 37 27 26 25 24
24 15
BspEI T'CCGG_A
Cuts at: 5349 5349
Size: 5686
BspHI T'CATG_A
Cuts at: 3575 5305 3575
Size: 1730 3956
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2043 2508 2784 3041 844
Size: 1199 465 276 257 3489
Fragments arranged by size:
3489 1199 465 276 257
BsrBI CCG'CTC
Cuts at: 1394 1823 3573 1394
Size: 429 1750 3507
Fragments arranged by size:
3507 1750 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2478 2653 4181 4355 65
Size: 1183 1230 175 1528 174 1396
Fragments arranged by size:
1528 1396 1230 1183 175 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2637 2700 4330 4517 1700
Size: 110 827 63 1630 187 2869
Fragments arranged by size:
2869 1630 827 187 110 63
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5686
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5686
BssSI C'ACGA_G
Cuts at: 3746 4758 3746
Size: 1012 4674
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5686
BstBI TT'CG_AA
Cuts at: 4490 4490
Size: 5686
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5686
BstYI r'GATC_y
Cuts at: 1972 2373 3016 3383 3768 3785 5237 5323 5335
Size: 401 643 367 385 17 1452 86 12
Cuts at: 5335 5681 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 643 401 385 367 346 86
17 12
Bsu36I CC'TnA_GG
Cuts at: 2538 2538
Size: 5686
BtgI C'CryG_G
Cuts at: 513 1065 513
Size: 552 5134
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2800 487
Size: 683 56 1574 3373
Fragments arranged by size:
3373 1574 683 56
BtsI GCAGTG_nn'
Cuts at: 2298 2895 3202 3996 4016 2298
Size: 597 307 794 20 3968
Fragments arranged by size:
3968 794 597 307 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5686
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5651
DraI TTT'AAA
Cuts at: 2990 2990
Size: 5686
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4693 809
Size: 507 3377 1802
Fragments arranged by size:
3377 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3133 4022 5651
Size: 54 1425 50 275 18 1303 889 1629
Cuts at: 5651 8
Size: 43
Fragments arranged by size:
1629 1425 1303 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 3133 1537
Size: 275 1321 4090
Fragments arranged by size:
4090 1321 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2792 3616 1353
Size: 1439 824 3423
Fragments arranged by size:
3423 1439 824
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2723 2809 4273 4647 4793 387
Size: 1064 138 1134 86 1464 374 146 1280
Fragments arranged by size:
1464 1280 1134 1064 374 146 138 86
EcoICRI GAG'CTC
Cuts at: 2326 2572 2326
Size: 246 5440
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2398 2474 2708 3749 4346 5133 5401
Size: 393 766 76 234 1041 597 787 268
Cuts at: 5401 1239
Size: 1524
Fragments arranged by size:
1524 1041 787 766 597 393 268 234
76
EcoNI CCTnn'n_nnAGG
Cuts at: 2017 2017
Size: 5686
EcoO109I rG'GnC_Cy
Cuts at: 2213 2275 2276 2547 2916 2917 2213
Size: 62 1 271 369 1 4982
Fragments arranged by size:
4982 369 271 62 1 1
EcoRI G'AATT_C
Cuts at: 2995 3373 2995
Size: 378 5308
FspI TGC'GCA
Cuts at: 4192 4192
Size: 5686
HaeII r_GCGC'y
Cuts at: 1678 2435 4833 1678
Size: 757 2398 2531
Fragments arranged by size:
2531 2398 757
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2039 2071 4327 4359 4401
Size: 32 135 32 467 32 2256 32 42
Cuts at: 4401 4433 1373
Size: 32 2626
Fragments arranged by size:
2626 2256 467 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2497 3030 3276 669
Size: 988 281 559 533 246 3079
Fragments arranged by size:
3079 988 559 533 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5686
HpaI GTT'AAC
Cuts at: 3276 3276
Size: 5686
KpnI G_GTAC'C
Cuts at: 3011 3011
Size: 5686
MfeI C'AATT_G
Cuts at: 3285 3285
Size: 5686
MluI A'CGCG_T
Cuts at: 5562 5562
Size: 5686
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2682 2925 4800 4984 1305
Size: 1377 243 1875 184 2007
Fragments arranged by size:
2007 1875 1377 243 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3864
Fragments arranged by size:
3864 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2390 2441 2483 2778 2971 3644 4003
Size: 648 1224 51 42 295 193 673 359
Cuts at: 4003 4162 518
Size: 159 2042
Fragments arranged by size:
2042 1224 673 648 359 295 193 159
51 42
MspA1I CmG'CkG
Cuts at: 1606 2035 2160 3102 3779 4927 5172 1606
Size: 429 125 942 677 1148 245 2120
Fragments arranged by size:
2120 1148 942 677 429 245 125
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5134
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5686
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5686
NotI GC'GGCC_GC
Cuts at: 3133 3133
Size: 5686
NspI r_CATG'y
Cuts at: 1132 1229 2350 3044 4589 1132
Size: 97 1121 694 1545 2229
Fragments arranged by size:
2229 1545 1121 694 97
PasI CC'CwG_GG
Cuts at: 1348 2040 1348
Size: 692 4994
PciI A'CATG_T
Cuts at: 4585 4585
Size: 5686
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2287 2970 1108
Size: 1179 683 3824
Fragments arranged by size:
3824 1179 683
PfoI T'CCnGG_A
Cuts at: 2451 2451
Size: 5686
PmeI GTTT'AAAC
Cuts at: 2990 2990
Size: 5686
PmlI CAC'GTG
Cuts at: 2557 2557
Size: 5686
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3755 3787 5268 5300 3755
Size: 32 1481 32 4141
Fragments arranged by size:
4141 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 2600 1613
Size: 987 4699
PsiI TTA'TAA
Cuts at: 3256 3256
Size: 5686
PspOMI G'GGCC_C
Cuts at: 2212 2275 2546 2916 2212
Size: 63 271 370 4982
Fragments arranged by size:
4982 370 271 63
PstI C_TGCA'G
Cuts at: 830 2154 3038 830
Size: 1324 884 3478
Fragments arranged by size:
3478 1324 884
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5686
PvuII CAG'CTG
Cuts at: 2035 2160 2035
Size: 125 5561
SacI G_AGCT'C
Cuts at: 2328 2574 2328
Size: 246 5440
SalI G'TCGA_C
Cuts at: 1936 3028 1936
Size: 1092 4594
SbfI CC_TGCA'GG
Cuts at: 3038 3038
Size: 5686
ScaI AGT'ACT
Cuts at: 3934 5646 3934
Size: 1712 3974
SexAI A'CCwGG_T
Cuts at: 2400 2400
Size: 5686
SfcI C'TryA_G
Cuts at: 826 1045 2150 3034 4169 4850 5041 826
Size: 219 1105 884 1135 681 191 1471
Fragments arranged by size:
1471 1135 1105 884 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5686
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5686
SmaI CCC'GGG
Cuts at: 1872 2180 3013 1872
Size: 308 833 4545
Fragments arranged by size:
4545 833 308
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3790 4691 4953 820
Size: 197 926 1847 901 262 1553
Fragments arranged by size:
1847 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3477 493
Size: 2984 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5686
SphI G_CATG'C
Cuts at: 1132 3044 1132
Size: 1912 3774
SrfI GCCC'GGGC
Cuts at: 2180 2180
Size: 5686
SspI AAT'ATT
Cuts at: 5 52 3610 5
Size: 47 3558 2081
Fragments arranged by size:
3558 2081 47
StuI AGG'CCT
Cuts at: 1517 2862 1517
Size: 1345 4341
StyI C'CwwG_G
Cuts at: 513 1065 3125 513
Size: 552 2060 3074
Fragments arranged by size:
3074 2060 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3713 3730 3883 4068 4513 325
Size: 1255 2133 17 153 185 445 1498
Fragments arranged by size:
2133 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3932 5644 96
Size: 274 80 33 51 1443 1955 1712 138
Fragments arranged by size:
1955 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2546 2578 5646 5678 2546
Size: 32 3068 32 2554
Fragments arranged by size:
3068 2554 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5686
XbaI T'CTAG_A
Cuts at: 3022 3022
Size: 5686
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5686
XmaI C'CCGG_G
Cuts at: 1870 2178 3011 1870
Size: 308 833 4545
Fragments arranged by size:
4545 833 308
XmnI GAAnn'nnTTC
Cuts at: 3815 3815
Size: 5686
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3494 276
Size: 53 83 186 936 1960 2468
Fragments arranged by size:
2468 1960 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII
BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI
EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI
KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI
NdeI NheI NotI NspI PasI PciI PflMI PfoI
PmeI PmlI PpiI PshAI PsiI PspOMI PstI PvuI
PvuII SacI SalI SbfI ScaI SexAI SfcI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SrfI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AscI AvrII BaeI BbeI BbvCI BfrBI BsiWI BsmBI
BstXI BstZ17I DraIII EcoRV FalI FseI FspAI KasI
NaeI NarI NgoMIV NruI NsiI PacI PpuMI PspXI
PsrI RsrII SacII SanDI SapI SfiI SfoI SwaI
XcmI