Restriction map for
pFN21AB5438
(Circular) (Six-base) MAPSORT of: pFN21AB5438.seq Check: 9053 from: 1 to: 6031
pFN21AB5438 6031 bp
With 182 enzymes: SgfI *
January 16, 2009 15:25 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 2346 3841 278
Size: 53 83 186 936 810 1495 2468
Fragments arranged by size:
2468 1495 936 810 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2280 2574 2625 3374 1937
Size: 343 294 51 749 4594
Fragments arranged by size:
4594 749 343 294 51
Acc65I G'GTAC_C
Cuts at: 3352 3352
Size: 6031
AclI AA'CG_TT
Cuts at: 1563 4158 4531 1563
Size: 2595 373 3063
Fragments arranged by size:
3063 2595 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3335 4094 5478 5746 1632
Size: 1703 759 1384 268 1917
Fragments arranged by size:
1917 1703 1384 759 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5834
AflIII A'CryG_T
Cuts at: 1203 4930 5907 1203
Size: 3727 977 1327
Fragments arranged by size:
3727 1327 977
AgeI A'CCGG_T
Cuts at: 4862 4862
Size: 6031
AhdI GACnn_n'nnGTC
Cuts at: 4760 4760
Size: 6031
AleI CACnn'nnGTG
Cuts at: 1166 2405 1166
Size: 1239 4792
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6031
AlwNI CAG_nnn'CTG
Cuts at: 2836 3774 5346 2836
Size: 938 1572 3521
Fragments arranged by size:
3521 1572 938
ApaI G_GGCC'C
Cuts at: 2704 2704
Size: 6031
ApaLI G'TGCA_C
Cuts at: 2353 4088 5244 2353
Size: 1735 1156 3140
Fragments arranged by size:
3140 1735 1156
ApoI r'AATT_y
Cuts at: 1493 2303 3340 3566 3718 5827 1493
Size: 810 1037 226 152 2109 1697
Fragments arranged by size:
2109 1697 1037 810 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4585 160
Size: 872 3553 1606
Fragments arranged by size:
3553 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6031
AvaI C'yCGr_G
Cuts at: 1870 1943 2741 2870 3356 1870
Size: 73 798 129 486 4545
Fragments arranged by size:
4545 798 486 129 73
BamHI G'GATC_C
Cuts at: 2008 3361 3728 2008
Size: 1353 367 4311
Fragments arranged by size:
4311 1353 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3352 4807 618
Size: 325 217 627 1565 1455 1842
Fragments arranged by size:
1842 1565 1455 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2704 2716 2830 3350 1391
Size: 1313 12 114 520 4072
Fragments arranged by size:
4072 1313 520 114 12
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2143 928
Size: 971 244 4816
Fragments arranged by size:
4816 971 244
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4222 4256 1903
Size: 34 2285 34 3678
Fragments arranged by size:
3678 2285 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3923 5133 3923
Size: 1210 4821
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6031
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2561 4642 136
Size: 107 122 71 631 605 889 2081 1525
Fragments arranged by size:
2081 1525 889 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2634 3018 6026 2634
Size: 384 3008 2639
Fragments arranged by size:
3008 2639 384
BlpI GC'TnA_GC
Cuts at: 3448 3448
Size: 6031
Bme1580I G_kGCm'C
Cuts at: 1790 2357 2604 2704 4092 5248 1790
Size: 567 247 100 1388 1156 2573
Fragments arranged by size:
2573 1388 1156 567 247 100
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6031
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2786 3762 4720
Size: 416 53 83 399 24 1369 976 958
Cuts at: 4720 442
Size: 1753
Fragments arranged by size:
1753 1369 976 958 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6031
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2869 2869
Size: 6031
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2063 2077 2306 2839 2858 4691 1239
Size: 824 14 229 533 19 1833 2579
Fragments arranged by size:
2579 1833 824 533 229 19 14
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3050 4156 5021 5319 3050
Size: 1106 865 298 3762
Fragments arranged by size:
3762 1106 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2948 4694 882
Size: 617 1449 1746 2219
Fragments arranged by size:
2219 1746 1449 617
BsaAI yAC'GTr
Cuts at: 493 1204 2599 3237 3822 493
Size: 711 1395 638 585 2702
Fragments arranged by size:
2702 1395 711 638 585
BsaBI GATnn'nnATC
Cuts at: 2037 2471 6025 2037
Size: 434 3554 2043
Fragments arranged by size:
3554 2043 434
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2343 2656 3838 4220
Size: 53 83 186 936 810 313 1182 382
Cuts at: 4220 4855 275
Size: 635 1451
Fragments arranged by size:
1451 1182 936 810 635 382 313 186
83 53
BsaWI w'CCGG_w
Cuts at: 4464 4862 5136 5283 5694 4464
Size: 398 274 147 411 4801
Fragments arranged by size:
4801 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5806
Fragments arranged by size:
5806 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2237 2501 2569 1186
Size: 1051 264 68 4648
Fragments arranged by size:
4648 1051 264 68
BseYI C'CCAG_C
Cuts at: 2900 3093 3241 5234 5730 2900
Size: 193 148 1993 496 3201
Fragments arranged by size:
3201 1993 496 193 148
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2743 1163
Size: 573 1007 4451
Fragments arranged by size:
4451 1007 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3481 4242 5270 1540
Size: 275 186 1480 761 1028 2301
Fragments arranged by size:
2301 1480 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2357 4092 4177 5248 2357
Size: 1735 85 1071 3140
Fragments arranged by size:
3140 1735 1071 85
BsmI GAATG_Cn'
Cuts at: 3542 3635 3542
Size: 93 5938
BsmBI CGTCTCn'nnnn_
Cuts at: 2593 2593
Size: 6031
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2357 2604 2704 2716 2830 3350 4092
Size: 399 567 247 100 12 114 520 742
Cuts at: 4092 4177 5248 1391
Size: 85 1071 2174
Fragments arranged by size:
2174 1071 742 567 520 399 247 114
100 85 12
BspEI T'CCGG_A
Cuts at: 5694 5694
Size: 6031
BspHI T'CATG_A
Cuts at: 2346 2463 2769 3920 5650 2346
Size: 117 306 1151 1730 2727
Fragments arranged by size:
2727 1730 1151 306 117
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3386 844
Size: 2542 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3918 1394
Size: 429 2095 3507
Fragments arranged by size:
3507 2095 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2550 4526 4700 65
Size: 1183 1302 1976 174 1396
Fragments arranged by size:
1976 1396 1302 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2061 4675 4862 1700
Size: 110 251 2614 187 2869
Fragments arranged by size:
2869 2614 251 187 110
BsrGI T'GTAC_A
Cuts at: 96 2424 3222 96
Size: 2328 798 2905
Fragments arranged by size:
2905 2328 798
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6031
BssSI C'ACGA_G
Cuts at: 4091 5103 4091
Size: 1012 5019
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6031
BstBI TT'CG_AA
Cuts at: 4835 4835
Size: 6031
BstEII G'GTnAC_C
Cuts at: 1184 2455 1184
Size: 1271 4760
BstYI r'GATC_y
Cuts at: 1972 2008 2634 3018 3027 3318 3361 3728 4113
Size: 36 626 384 9 291 43 367 385
Cuts at: 4113 4130 5582 5668 5680 6026 1972
Size: 17 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 626 385 384 367 346 291
86 43 36 17 12 9
Bsu36I CC'TnA_GG
Cuts at: 2708 2708
Size: 6031
BtgI C'CryG_G
Cuts at: 513 1065 2004 2938 513
Size: 552 939 934 3606
Fragments arranged by size:
3606 939 934 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2122 487
Size: 683 56 896 4396
Fragments arranged by size:
4396 896 683 56
BtsI GCAGTG_nn'
Cuts at: 2120 2241 3325 3547 4341 4361 2120
Size: 121 1084 222 794 20 3790
Fragments arranged by size:
3790 1084 794 222 121 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6031
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5996
DraI TTT'AAA
Cuts at: 3335 3335
Size: 6031
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2249 2330 5038 809
Size: 507 933 81 2708 1802
Fragments arranged by size:
2708 1802 933 507 81
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2238 2409 3478
Size: 54 1425 50 275 18 408 171 1069
Cuts at: 3478 4367 5996 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 1069 889 408 275 171 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3478 1537
Size: 275 1666 4090
Fragments arranged by size:
4090 1666 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2519 3961 1353
Size: 1166 1442 3423
Fragments arranged by size:
3423 1442 1166
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2105 2818 4618 4992 5138 387
Size: 1064 138 516 713 1800 374 146 1280
Fragments arranged by size:
1800 1280 1064 713 516 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2063 2077 2306 2839 2858 3335 4094
Size: 393 431 14 229 533 19 477 759
Cuts at: 4094 4691 5478 5746 1239
Size: 597 787 268 1524
Fragments arranged by size:
1524 787 759 597 533 477 431 393
268 229 19 14
EcoO109I rG'GnC_Cy
Cuts at: 2482 2700 2877 2482
Size: 218 177 5636
Fragments arranged by size:
5636 218 177
EcoRI G'AATT_C
Cuts at: 3340 3718 3340
Size: 378 5653
FspI TGC'GCA
Cuts at: 4537 4537
Size: 6031
HaeII r_GCGC'y
Cuts at: 1678 2198 2452 3231 3261 5178 1678
Size: 520 254 779 30 1917 2531
Fragments arranged by size:
2531 1917 779 520 254 30
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 4672 4704 4746 4778 1373
Size: 32 135 32 3100 32 42 32 2626
Fragments arranged by size:
3100 2626 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2671 3253 3375 3621 669
Size: 988 281 733 582 122 246 3079
Fragments arranged by size:
3079 988 733 582 281 246 122
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6031
HpaI GTT'AAC
Cuts at: 3621 3621
Size: 6031
KpnI G_GTAC'C
Cuts at: 3356 3356
Size: 6031
MfeI C'AATT_G
Cuts at: 3630 3630
Size: 6031
MluI A'CGCG_T
Cuts at: 5907 5907
Size: 6031
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5145 5329 1305
Size: 3840 184 2007
Fragments arranged by size:
3840 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2411 10
Size: 54 1425 343 579 3630
Fragments arranged by size:
3630 1425 579 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2177 2405 2417 2462 3119 3989 4348
Size: 648 1011 228 12 45 657 870 359
Cuts at: 4348 4507 518
Size: 159 2042
Fragments arranged by size:
2042 1011 870 657 648 359 228 159
45 12
MspA1I CmG'CkG
Cuts at: 1606 2405 2914 3447 4124 5272 5517 1606
Size: 799 509 533 677 1148 245 2120
Fragments arranged by size:
2120 1148 799 677 533 509 245
NaeI GCC'GGC
Cuts at: 2063 2063
Size: 6031
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4540
Fragments arranged by size:
4540 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6031
NgoMIV G'CCGG_C
Cuts at: 2061 2061
Size: 6031
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6031
NotI GC'GGCC_GC
Cuts at: 3478 3478
Size: 6031
NspI r_CATG'y
Cuts at: 1132 1229 2993 3389 4934 1132
Size: 97 1764 396 1545 2229
Fragments arranged by size:
2229 1764 1545 396 97
PasI CC'CwG_GG
Cuts at: 1348 2271 1348
Size: 923 5108
PciI A'CATG_T
Cuts at: 4930 4930
Size: 6031
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2478 2836 1108
Size: 1370 358 4303
Fragments arranged by size:
4303 1370 358
PmeI GTTT'AAAC
Cuts at: 3335 3335
Size: 6031
PmlI CAC'GTG
Cuts at: 3237 3237
Size: 6031
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4100 4132 5613 5645 4100
Size: 32 1481 32 4486
Fragments arranged by size:
4486 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2482 2877 2482
Size: 395 5636
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6031
PsiI TTA'TAA
Cuts at: 3601 3601
Size: 6031
PspOMI G'GGCC_C
Cuts at: 2700 2700
Size: 6031
PstI C_TGCA'G
Cuts at: 830 2202 3383 830
Size: 1372 1181 3478
Fragments arranged by size:
3478 1372 1181
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6031
PvuII CAG'CTG
Cuts at: 2405 2914 2405
Size: 509 5522
SalI G'TCGA_C
Cuts at: 1936 3373 1936
Size: 1437 4594
SbfI CC_TGCA'GG
Cuts at: 3383 3383
Size: 6031
ScaI AGT'ACT
Cuts at: 4279 5991 4279
Size: 1712 4319
SfcI C'TryA_G
Cuts at: 826 1045 2198 2437 3379 4514 5195 5386 826
Size: 219 1153 239 942 1135 681 191 1471
Fragments arranged by size:
1471 1153 1135 942 681 239 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6031
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6031
SmaI CCC'GGG
Cuts at: 1872 3358 1872
Size: 1486 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3065 4135 5036 5298 820
Size: 197 926 1122 1070 901 262 1553
Fragments arranged by size:
1553 1122 1070 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3822 493
Size: 3329 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6031
SphI G_CATG'C
Cuts at: 1132 2993 3389 1132
Size: 1861 396 3774
Fragments arranged by size:
3774 1861 396
SspI AAT'ATT
Cuts at: 5 52 3955 5
Size: 47 3903 2081
Fragments arranged by size:
3903 2081 47
StuI AGG'CCT
Cuts at: 1517 3308 1517
Size: 1791 4240
StyI C'CwwG_G
Cuts at: 513 1065 2004 3470 513
Size: 552 939 1466 3074
Fragments arranged by size:
3074 1466 939 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2914 4058 4075 4228 4413 4858 325
Size: 1255 1334 1144 17 153 185 445 1498
Fragments arranged by size:
1498 1334 1255 1144 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2424 2732 3222
Size: 274 80 33 51 1443 447 308 490
Cuts at: 3222 4277 5989 96
Size: 1055 1712 138
Fragments arranged by size:
1712 1443 1055 490 447 308 274 138
80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2305 2337 5991 6023 2305
Size: 32 3654 32 2313
Fragments arranged by size:
3654 2313 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6031
XbaI T'CTAG_A
Cuts at: 3367 3367
Size: 6031
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6031
XmaI C'CCGG_G
Cuts at: 1870 3356 1870
Size: 1486 4545
XmnI GAAnn'nnTTC
Cuts at: 4160 4160
Size: 6031
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 2344 3839 276
Size: 53 83 186 936 810 1495 2468
Fragments arranged by size:
2468 1495 936 810 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI
BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI
EarI EciI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I
HincII HindIII HpaI KpnI MfeI MluI MmeI MscI
MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI
NspI PasI PciI PflMI PmeI PmlI PpiI PpuMI
PshAI PsiI PspOMI PstI PvuI PvuII SalI SbfI
ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI AscI AvrII BaeI BbeI BbvCI
BfrBI Bpu10I BsiWI BstXI BstZ17I DraIII EcoICRI EcoNI
EcoRV FalI FseI FspAI KasI NarI NruI NsiI
PacI PfoI PspXI PsrI RsrII SacI SacII SanDI
SapI SexAI SfiI SfoI SrfI SwaI XcmI