Restriction map for
pFN21AB5437
(Circular) (Six-base) MAPSORT of: pFN21AB5437.seq Check: 532 from: 1 to: 5704
pFN21AB5437 5704 bp
With 182 enzymes: SgfI *
April 15, 2010 09:31 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3514 278
Size: 53 83 186 936 1978 2468
Fragments arranged by size:
2468 1978 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3047 1937
Size: 1110 4594
Acc65I G'GTAC_C
Cuts at: 3025 3025
Size: 5704
AclI AA'CG_TT
Cuts at: 1563 3831 4204 1563
Size: 2268 373 3063
Fragments arranged by size:
3063 2268 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3767 5151 5419 1632
Size: 2135 1384 268 1917
Fragments arranged by size:
2135 1917 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5507
AflIII A'CryG_T
Cuts at: 1203 4603 5580 1203
Size: 3400 977 1327
Fragments arranged by size:
3400 1327 977
AgeI A'CCGG_T
Cuts at: 4535 4535
Size: 5704
AhdI GACnn_n'nnGTC
Cuts at: 4433 4433
Size: 5704
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5704
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5704
AlwNI CAG_nnn'CTG
Cuts at: 2027 2355 2396 2660 3447 5019 2027
Size: 328 41 264 787 1572 2712
Fragments arranged by size:
2712 1572 787 328 264 41
ApaLI G'TGCA_C
Cuts at: 3761 4917 3761
Size: 1156 4548
ApoI r'AATT_y
Cuts at: 1493 2507 3013 3239 3391 5500 1493
Size: 1014 506 226 152 2109 1697
Fragments arranged by size:
2109 1697 1014 506 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4258 160
Size: 872 3226 1606
Fragments arranged by size:
3226 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5704
AvaI C'yCGr_G
Cuts at: 1870 1943 3029 1870
Size: 73 1086 4545
Fragments arranged by size:
4545 1086 73
BamHI G'GATC_C
Cuts at: 3034 3401 3034
Size: 367 5337
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3025 4480 618
Size: 325 217 627 1238 1455 1842
Fragments arranged by size:
1842 1455 1238 627 325 217
BanII G_rGCy'C
Cuts at: 1391 3023 1391
Size: 1632 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4733
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3895 3929 1903
Size: 34 1958 34 3678
Fragments arranged by size:
3678 1958 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3596 4806 3596
Size: 1210 4494
BclI T'GATC_A
Cuts at: 1548 2472 1548
Size: 924 4780
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2009 4315 136
Size: 107 122 71 631 605 337 2306 1525
Fragments arranged by size:
2306 1525 631 605 337 122 107 71
BglII A'GATC_T
Cuts at: 2853 5699 2853
Size: 2846 2858
BlpI GC'TnA_GC
Cuts at: 3121 3121
Size: 5704
Bme1580I G_kGCm'C
Cuts at: 1790 3765 4921 1790
Size: 1975 1156 2573
Fragments arranged by size:
2573 1975 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5704
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3435 4393 442
Size: 416 53 83 399 24 2018 958 1753
Fragments arranged by size:
2018 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5704
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2571 4364 1239
Size: 1332 1793 2579
Fragments arranged by size:
2579 1793 1332
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2436 3829 4694 4992 2436
Size: 1393 865 298 3148
Fragments arranged by size:
3148 1393 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4367 882
Size: 617 2868 2219
Fragments arranged by size:
2868 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3495 493
Size: 711 2291 2702
Fragments arranged by size:
2702 2291 711
BsaBI GATnn'nnATC
Cuts at: 5698 5698
Size: 5704
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3511 3893 4528 275
Size: 53 83 186 936 1978 382 635 1451
Fragments arranged by size:
1978 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4137 4535 4809 4956 5367 4137
Size: 398 274 147 411 4474
Fragments arranged by size:
4474 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5479
Fragments arranged by size:
5479 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2114 1186
Size: 928 4776
BseYI C'CCAG_C
Cuts at: 4907 5403 4907
Size: 496 5208
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2355 1163
Size: 573 619 4512
Fragments arranged by size:
4512 619 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3154 3915 4943 1540
Size: 275 186 1153 761 1028 2301
Fragments arranged by size:
2301 1153 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2609 3765 3850 4921 2609
Size: 1156 85 1071 3392
Fragments arranged by size:
3392 1156 1071 85
BsmI GAATG_Cn'
Cuts at: 3215 3308 3215
Size: 93 5611
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2609 3023 3765 3850 4921 1391
Size: 399 819 414 742 85 1071 2174
Fragments arranged by size:
2174 1071 819 742 414 399 85
BspEI T'CCGG_A
Cuts at: 5367 5367
Size: 5704
BspHI T'CATG_A
Cuts at: 3593 5323 3593
Size: 1730 3974
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3059 844
Size: 2215 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3591 1394
Size: 429 1768 3507
Fragments arranged by size:
3507 1768 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4199 4373 65
Size: 1183 2951 174 1396
Fragments arranged by size:
2951 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4348 4535 1700
Size: 110 2538 187 2869
Fragments arranged by size:
2869 2538 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5704
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5704
BssSI C'ACGA_G
Cuts at: 3764 4776 3764
Size: 1012 4692
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2027 794
Size: 1233 4471
BstBI TT'CG_AA
Cuts at: 4508 4508
Size: 5704
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5704
BstYI r'GATC_y
Cuts at: 1972 2853 3034 3401 3786 3803 5255 5341 5353
Size: 881 181 367 385 17 1452 86 12
Cuts at: 5353 5699 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 881 385 367 346 181 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4213
Fragments arranged by size:
4213 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4965
Fragments arranged by size:
4965 683 56
BtsI GCAGTG_nn'
Cuts at: 2241 3220 4014 4034 2241
Size: 979 794 20 3911
Fragments arranged by size:
3911 979 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5704
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5669
DraI TTT'AAA
Cuts at: 2614 2835 3008 2614
Size: 221 173 5310
Fragments arranged by size:
5310 221 173
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2413 4711 809
Size: 507 1097 2298 1802
Fragments arranged by size:
2298 1802 1097 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3151 4040 5669
Size: 54 1425 50 275 18 1321 889 1629
Cuts at: 5669 8
Size: 43
Fragments arranged by size:
1629 1425 1321 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 3151 1537
Size: 275 1339 4090
Fragments arranged by size:
4090 1339 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3634 1353
Size: 2281 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4291 4665 4811 387
Size: 1064 138 2702 374 146 1280
Fragments arranged by size:
2702 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2571 3767 4364 5151 5419 1239
Size: 393 939 1196 597 787 268 1524
Fragments arranged by size:
1524 1196 939 787 597 393 268
EcoO109I rG'GnC_Cy
Cuts at: 2080 2080
Size: 5704
EcoRI G'AATT_C
Cuts at: 3013 3391 3013
Size: 378 5326
FspI TGC'GCA
Cuts at: 4210 4210
Size: 5704
HaeII r_GCGC'y
Cuts at: 1678 4851 1678
Size: 3173 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 4345 4377 4419 4451 1373
Size: 32 135 32 2773 32 42 32 2626
Fragments arranged by size:
2773 2626 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3048 3294 669
Size: 988 281 1110 246 3079
Fragments arranged by size:
3079 1110 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5704
HpaI GTT'AAC
Cuts at: 3294 3294
Size: 5704
KpnI G_GTAC'C
Cuts at: 3029 3029
Size: 5704
MfeI C'AATT_G
Cuts at: 3303 3303
Size: 5704
MluI A'CGCG_T
Cuts at: 5580 5580
Size: 5704
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2796 4818 5002 1305
Size: 1491 2022 184 2007
Fragments arranged by size:
2022 2007 1491 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3882
Fragments arranged by size:
3882 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2246 3662 4021 4180 518
Size: 648 1080 1416 359 159 2042
Fragments arranged by size:
2042 1416 1080 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 3120 3797 4945 5190 1606
Size: 1514 677 1148 245 2120
Fragments arranged by size:
2120 1514 1148 677 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4213
Fragments arranged by size:
4213 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5704
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5704
NotI GC'GGCC_GC
Cuts at: 3151 3151
Size: 5704
NspI r_CATG'y
Cuts at: 1132 1229 3062 4607 1132
Size: 97 1833 1545 2229
Fragments arranged by size:
2229 1833 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5704
PciI A'CATG_T
Cuts at: 4603 4603
Size: 5704
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5704
PmeI GTTT'AAAC
Cuts at: 3008 3008
Size: 5704
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3773 3805 5286 5318 3773
Size: 32 1481 32 4159
Fragments arranged by size:
4159 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2080 2080
Size: 5704
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5704
PsiI TTA'TAA
Cuts at: 3274 3274
Size: 5704
PstI C_TGCA'G
Cuts at: 830 2020 3056 830
Size: 1190 1036 3478
Fragments arranged by size:
3478 1190 1036
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5704
SalI G'TCGA_C
Cuts at: 1936 3046 1936
Size: 1110 4594
SbfI CC_TGCA'GG
Cuts at: 3056 3056
Size: 5704
ScaI AGT'ACT
Cuts at: 2619 3952 5664 2619
Size: 1333 1712 2659
Fragments arranged by size:
2659 1712 1333
SfcI C'TryA_G
Cuts at: 826 1045 2016 3052 4187 4868 5059 826
Size: 219 971 1036 1135 681 191 1471
Fragments arranged by size:
1471 1135 1036 971 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5704
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5704
SmaI CCC'GGG
Cuts at: 1872 3031 1872
Size: 1159 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2451 3808 4709 4971 820
Size: 197 926 508 1357 901 262 1553
Fragments arranged by size:
1553 1357 926 901 508 262 197
SnaBI TAC'GTA
Cuts at: 493 3495 493
Size: 3002 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5704
SphI G_CATG'C
Cuts at: 1132 3062 1132
Size: 1930 3774
SspI AAT'ATT
Cuts at: 5 52 3628 5
Size: 47 3576 2081
Fragments arranged by size:
3576 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5704
StyI C'CwwG_G
Cuts at: 513 1065 2004 3143 513
Size: 552 939 1139 3074
Fragments arranged by size:
3074 1139 939 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3731 3748 3901 4086 4531 325
Size: 1255 2151 17 153 185 445 1498
Fragments arranged by size:
2151 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2617 3950 5662
Size: 274 80 33 51 1443 640 1333 1712
Cuts at: 5662 96
Size: 138
Fragments arranged by size:
1712 1443 1333 640 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2173 2205 5664 5696 2173
Size: 32 3459 32 2181
Fragments arranged by size:
3459 2181 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5704
XbaI T'CTAG_A
Cuts at: 2218 3040 2218
Size: 822 4882
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5704
XmaI C'CCGG_G
Cuts at: 1870 3029 1870
Size: 1159 4545
XmnI GAAnn'nnTTC
Cuts at: 2580 3833 2580
Size: 1253 4451
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3512 276
Size: 53 83 186 936 1978 2468
Fragments arranged by size:
2468 1978 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BcgI BciVI BclI
BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI
BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI
DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I
EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI
MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI
NheI NotI NspI PasI PciI PflMI PmeI PpiI
PpuMI PshAI PsiI PstI PvuI SalI SbfI ScaI
SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstXI BstZ17I
Bsu36I DraIII EcoICRI EcoNI EcoRV FalI FseI FspAI
KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI
PmlI PspOMI PspXI PsrI PvuII RsrII SacI SacII
SanDI SapI SexAI SfiI SfoI SrfI SwaI XcmI