Restriction map for
pFN21AB5432
(Circular) (Six-base) MAPSORT of: pFN21AB5432.seq Check: 9787 from: 1 to: 6532
pFN21AB5432 6532 bp
With 182 enzymes: SgfI *
October 20, 2009 10:33 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 3367 3367
Size: 6532
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4342 278
Size: 53 83 186 936 2806 2468
Fragments arranged by size:
2806 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3875 1937
Size: 1938 4594
Acc65I G'GTAC_C
Cuts at: 2270 3853 2270
Size: 1583 4949
AclI AA'CG_TT
Cuts at: 1563 2392 4659 5032 1563
Size: 829 2267 373 3063
Fragments arranged by size:
3063 2267 829 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2639 2807 3092 3448 3633 3716 3794 3806
Size: 1007 168 285 356 185 83 78 12
Cuts at: 3806 4595 5979 6247 1632
Size: 789 1384 268 1917
Fragments arranged by size:
1917 1384 1007 789 356 285 268 185
168 83 78 12
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6335
AflIII A'CryG_T
Cuts at: 1203 5431 6408 1203
Size: 4228 977 1327
Fragments arranged by size:
4228 1327 977
AgeI A'CCGG_T
Cuts at: 5363 5363
Size: 6532
AhdI GACnn_n'nnGTC
Cuts at: 5261 5261
Size: 6532
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6532
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 3211 1184
Size: 2027 4505
AlwNI CAG_nnn'CTG
Cuts at: 2326 3799 4275 5847 2326
Size: 1473 476 1572 3011
Fragments arranged by size:
3011 1572 1473 476
ApaLI G'TGCA_C
Cuts at: 4589 5745 4589
Size: 1156 5376
ApoI r'AATT_y
Cuts at: 1493 2287 2296 2349 3276 3841 4067 4219 6328
Size: 794 9 53 927 565 226 152 2109
Cuts at: 6328 1493
Size: 1697
Fragments arranged by size:
2109 1697 927 794 565 226 152 53
9
AseI AT'TA_AT
Cuts at: 160 1032 5086 160
Size: 872 4054 1606
Fragments arranged by size:
4054 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6532
AvaI C'yCGr_G
Cuts at: 1870 1943 3857 1870
Size: 73 1914 4545
Fragments arranged by size:
4545 1914 73
BamHI G'GATC_C
Cuts at: 3862 4229 3862
Size: 367 6165
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2270 3244 3853 5308 618
Size: 325 217 627 483 974 609 1455 1842
Fragments arranged by size:
1842 1455 974 627 609 483 325 217
BanII G_rGCy'C
Cuts at: 1391 3140 3563 3851 1391
Size: 1749 423 288 4072
Fragments arranged by size:
4072 1749 423 288
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2526 2730 3704 928
Size: 971 627 204 974 3756
Fragments arranged by size:
3756 974 971 627 204
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4723 4757 1903
Size: 34 2786 34 3678
Fragments arranged by size:
3678 2786 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2213 4424 5634 2213
Size: 2211 1210 3111
Fragments arranged by size:
3111 2211 1210
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6532
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2141 2558 3761
Size: 107 122 71 631 605 469 417 1203
Cuts at: 3761 5143 136
Size: 1382 1525
Fragments arranged by size:
1525 1382 1203 631 605 469 417 122
107 71
BglII A'GATC_T
Cuts at: 2245 2964 6527 2245
Size: 719 3563 2250
Fragments arranged by size:
3563 2250 719
BlpI GC'TnA_GC
Cuts at: 3949 3949
Size: 6532
Bme1580I G_kGCm'C
Cuts at: 1790 3741 4593 5749 1790
Size: 1951 852 1156 2573
Fragments arranged by size:
2573 1951 1156 852
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6532
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3566 4263 5221
Size: 416 53 83 399 24 2149 697 958
Cuts at: 5221 442
Size: 1753
Fragments arranged by size:
2149 1753 958 697 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6532
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 3011 3194 3276 3354 3494 5192 1239
Size: 1772 183 82 78 140 1698 2579
Fragments arranged by size:
2579 1772 1698 183 140 82 78
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2831 4657 5522 5820 2831
Size: 1826 865 298 3543
Fragments arranged by size:
3543 1826 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2747 2964 5195 882
Size: 617 1248 217 2231 2219
Fragments arranged by size:
2231 2219 1248 617 217
BsaAI yAC'GTr
Cuts at: 493 1204 4323 493
Size: 711 3119 2702
Fragments arranged by size:
3119 2702 711
BsaBI GATnn'nnATC
Cuts at: 2244 3606 6526 2244
Size: 1362 2920 2250
Fragments arranged by size:
2920 2250 1362
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4339 4721 5356 275
Size: 53 83 186 936 2806 382 635 1451
Fragments arranged by size:
2806 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4965 5363 5637 5784 6195 4965
Size: 398 274 147 411 5302
Fragments arranged by size:
5302 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 6307
Fragments arranged by size:
6307 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2844 3131 3279 3704 1186
Size: 1658 287 148 425 4014
Fragments arranged by size:
4014 1658 425 287 148
BseYI C'CCAG_C
Cuts at: 3721 5735 6231 3721
Size: 2014 496 4022
Fragments arranged by size:
4022 2014 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 3217 1163
Size: 573 1481 4478
Fragments arranged by size:
4478 1481 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3982 4743 5771 1540
Size: 275 186 1981 761 1028 2301
Fragments arranged by size:
2301 1981 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2770 3140 3157 4593 4678 5749 2770
Size: 370 17 1436 85 1071 3553
Fragments arranged by size:
3553 1436 1071 370 85 17
BsmI GAATG_Cn'
Cuts at: 4043 4136 4043
Size: 93 6439
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2770 3140 3157 3563 3741 3851 4593
Size: 399 980 370 17 406 178 110 742
Cuts at: 4593 4678 5749 1391
Size: 85 1071 2174
Fragments arranged by size:
2174 1071 980 742 406 399 370 178
110 85 17
BspEI T'CCGG_A
Cuts at: 6195 6195
Size: 6532
BspHI T'CATG_A
Cuts at: 4421 6151 4421
Size: 1730 4802
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2766 3367 3887 844
Size: 1922 601 520 3489
Fragments arranged by size:
3489 1922 601 520
BsrBI CCG'CTC
Cuts at: 1394 1823 4419 1394
Size: 429 2596 3507
Fragments arranged by size:
3507 2596 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2542 3524 3549 5027 5201 65
Size: 1183 1294 982 25 1478 174 1396
Fragments arranged by size:
1478 1396 1294 1183 982 174 25
BsrFI r'CCGG_y
Cuts at: 1700 1810 2573 5176 5363 1700
Size: 110 763 2603 187 2869
Fragments arranged by size:
2869 2603 763 187 110
BsrGI T'GTAC_A
Cuts at: 96 2214 96
Size: 2118 4414
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6532
BssSI C'ACGA_G
Cuts at: 4592 5604 4592
Size: 1012 5520
BstAPI GCAn_nnn'nTGC
Cuts at: 794 3799 794
Size: 3005 3527
BstBI TT'CG_AA
Cuts at: 5336 5336
Size: 6532
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6532
BstYI r'GATC_y
Cuts at: 1972 2245 2417 2964 3862 4229 4614 4631 6083
Size: 273 172 547 898 367 385 17 1452
Cuts at: 6083 6169 6181 6527 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1452 898 547 385 367 346 273
172 86 17 12
Bsu36I CC'TnA_GG
Cuts at: 2690 3089 2690
Size: 399 6133
BtgI C'CryG_G
Cuts at: 513 1065 2413 2530 513
Size: 552 1348 117 4515
Fragments arranged by size:
4515 1348 552 117
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2055 487
Size: 683 56 829 4964
Fragments arranged by size:
4964 829 683 56
BtsI GCAGTG_nn'
Cuts at: 2242 3519 4048 4842 4862 2242
Size: 1277 529 794 20 3912
Fragments arranged by size:
3912 1277 794 529 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6532
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6497
DraI TTT'AAA
Cuts at: 2302 3836 2302
Size: 1534 4998
DraIII CAC_nnn'GTG
Cuts at: 2435 2435
Size: 6532
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5539 809
Size: 507 4223 1802
Fragments arranged by size:
4223 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3979 4868 6497
Size: 54 1425 50 275 18 2149 889 1629
Cuts at: 6497 8
Size: 43
Fragments arranged by size:
2149 1629 1425 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 3979 1537
Size: 275 2167 4090
Fragments arranged by size:
4090 2167 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2061 2101 3003 3252 3681 3770 4462 1353
Size: 708 40 902 249 429 89 692 3423
Fragments arranged by size:
3423 902 708 692 429 249 89 40
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5119 5493 5639 387
Size: 1064 138 3530 374 146 1280
Fragments arranged by size:
3530 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 3138 3138
Size: 6532
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2639 2807 3011 3092 3194 3276 3354
Size: 393 1007 168 204 81 102 82 78
Cuts at: 3354 3448 3494 3633 3716 3794 3806 4595 5192
Size: 94 46 139 83 78 12 789 597
Cuts at: 5192 5979 6247 1239
Size: 787 268 1524
Fragments arranged by size:
1524 1007 789 787 597 393 268 204
168 139 102 94 83 82 81 78
78 46 12
EcoO109I rG'GnC_Cy
Cuts at: 2686 3093 2686
Size: 407 6125
EcoRI G'AATT_C
Cuts at: 2349 3841 4219 2349
Size: 1492 378 4662
Fragments arranged by size:
4662 1492 378
EcoRV GAT'ATC
Cuts at: 2662 2662
Size: 6532
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2090 3361 3430 2090
Size: 1271 69 5192
Fragments arranged by size:
5192 1271 69
FspI TGC'GCA
Cuts at: 5038 5038
Size: 6532
HaeII r_GCGC'y
Cuts at: 1678 5679 1678
Size: 4001 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2649 2681 5173 5205 5247
Size: 32 135 32 1077 32 2492 32 42
Cuts at: 5247 5279 1373
Size: 32 2626
Fragments arranged by size:
2626 2492 1077 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2175 3876 4122 669
Size: 988 281 237 1701 246 3079
Fragments arranged by size:
3079 1701 988 281 246 237
HindIII A'AGCT_T
Cuts at: 748 3407 748
Size: 2659 3873
HpaI GTT'AAC
Cuts at: 4122 4122
Size: 6532
KpnI G_GTAC'C
Cuts at: 2274 3857 2274
Size: 1583 4949
MfeI C'AATT_G
Cuts at: 4131 4131
Size: 6532
MluI A'CGCG_T
Cuts at: 6408 6408
Size: 6532
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3243 5646 5830 1305
Size: 1938 2403 184 2007
Fragments arranged by size:
2403 2007 1938 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4710
Fragments arranged by size:
4710 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2535 3642 4490 4849 5008 518
Size: 648 1369 1107 848 359 159 2042
Fragments arranged by size:
2042 1369 1107 848 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 3304 3948 4625 5773 6018 1606
Size: 1698 644 677 1148 245 2120
Fragments arranged by size:
2120 1698 1148 677 644 245
NcoI C'CATG_G
Cuts at: 513 1065 2530 513
Size: 552 1465 4515
Fragments arranged by size:
4515 1465 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6532
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6532
NotI GC'GGCC_GC
Cuts at: 3979 3979
Size: 6532
NspI r_CATG'y
Cuts at: 1132 1229 3890 5435 1132
Size: 97 2661 1545 2229
Fragments arranged by size:
2661 2229 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6532
PciI A'CATG_T
Cuts at: 5431 5431
Size: 6532
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6532
PfoI T'CCnGG_A
Cuts at: 3825 3825
Size: 6532
PmeI GTTT'AAAC
Cuts at: 3836 3836
Size: 6532
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4601 4633 6114 6146 4601
Size: 32 1481 32 4987
Fragments arranged by size:
4987 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2686 2686
Size: 6532
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6532
PsiI TTA'TAA
Cuts at: 2826 4102 2826
Size: 1276 5256
PstI C_TGCA'G
Cuts at: 830 3456 3884 830
Size: 2626 428 3478
Fragments arranged by size:
3478 2626 428
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6532
PvuII CAG'CTG
Cuts at: 3304 3304
Size: 6532
SacI G_AGCT'C
Cuts at: 3140 3140
Size: 6532
SalI G'TCGA_C
Cuts at: 1936 3874 1936
Size: 1938 4594
SapI GCTCTTCn'nnn_
Cuts at: 3003 3003
Size: 6532
SbfI CC_TGCA'GG
Cuts at: 3884 3884
Size: 6532
ScaI AGT'ACT
Cuts at: 4780 6492 4780
Size: 1712 4820
SfcI C'TryA_G
Cuts at: 826 1045 2653 3452 3880 5015 5696 5887 826
Size: 219 1608 799 428 1135 681 191 1471
Fragments arranged by size:
1608 1471 1135 799 681 428 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6532
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6532
SmaI CCC'GGG
Cuts at: 1872 3859 1872
Size: 1987 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2846 4636 5537 5799 820
Size: 197 926 903 1790 901 262 1553
Fragments arranged by size:
1790 1553 926 903 901 262 197
SnaBI TAC'GTA
Cuts at: 493 4323 493
Size: 3830 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6532
SphI G_CATG'C
Cuts at: 1132 3890 1132
Size: 2758 3774
SspI AAT'ATT
Cuts at: 5 52 2099 2308 4456 5
Size: 47 2047 209 2148 2081
Fragments arranged by size:
2148 2081 2047 209 47
StuI AGG'CCT
Cuts at: 1517 3479 3755 1517
Size: 1962 276 4294
Fragments arranged by size:
4294 1962 276
StyI C'CwwG_G
Cuts at: 513 1065 2139 2530 2748 3480 3486 3531 3564
Size: 552 1074 391 218 732 6 45 33
Cuts at: 3564 3669 3971 513
Size: 105 302 3074
Fragments arranged by size:
3074 1074 732 552 391 302 218 105
45 33 6
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 4559 4576 4729 4914 5359 325
Size: 1255 2979 17 153 185 445 1498
Fragments arranged by size:
2979 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2214 2921 4778
Size: 274 80 33 51 1443 237 707 1857
Cuts at: 4778 6490 96
Size: 1712 138
Fragments arranged by size:
1857 1712 1443 707 274 237 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6492 6524 6492
Size: 32 6500
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6532
XbaI T'CTAG_A
Cuts at: 2353 3868 2353
Size: 1515 5017
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6532
XmaI C'CCGG_G
Cuts at: 1870 3857 1870
Size: 1987 4545
XmnI GAAnn'nnTTC
Cuts at: 4661 4661
Size: 6532
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4340 276
Size: 53 83 186 936 2806 2468
Fragments arranged by size:
2806 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI BtsI
ClaI CspCI DraI DraIII DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoO109I EcoRI EcoRV FalI FspI
HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI
MmeI MscI MslI MspA1I NcoI NdeI NheI NotI
NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI
PshAI PsiI PstI PvuI PvuII SacI SalI SapI
SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI
BfrBI BplI Bpu10I BsiWI BsmBI BstXI BstZ17I EcoNI
FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI
PacI PmlI PspOMI PspXI PsrI RsrII SacII SanDI
SexAI SfiI SfoI SrfI SwaI XcmI