Restriction map for
pFN21AB5427
(Circular) (Six-base) MAPSORT of: pFN21AB5427.seq Check: 9073 from: 1 to: 5839
pFN21AB5427 5839 bp
With 182 enzymes: SgfI *
April 15, 2010 09:31 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2563 2563
Size: 5839
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3649 278
Size: 53 83 186 936 2113 2468
Fragments arranged by size:
2468 2113 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3182 1937
Size: 1245 4594
Acc65I G'GTAC_C
Cuts at: 3160 3160
Size: 5839
AclI AA'CG_TT
Cuts at: 1563 3966 4339 1563
Size: 2403 373 3063
Fragments arranged by size:
3063 2403 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2045 2817 3902 5286 5554 1632
Size: 413 772 1085 1384 268 1917
Fragments arranged by size:
1917 1384 1085 772 413 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5642
AflIII A'CryG_T
Cuts at: 1203 4738 5715 1203
Size: 3535 977 1327
Fragments arranged by size:
3535 1327 977
AgeI A'CCGG_T
Cuts at: 4670 4670
Size: 5839
AhdI GACnn_n'nnGTC
Cuts at: 4568 4568
Size: 5839
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5839
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5839
AlwNI CAG_nnn'CTG
Cuts at: 2038 2980 3130 3582 5154 2038
Size: 942 150 452 1572 2723
Fragments arranged by size:
2723 1572 942 452 150
ApaLI G'TGCA_C
Cuts at: 3896 5052 3896
Size: 1156 4683
ApoI r'AATT_y
Cuts at: 1493 3148 3374 3526 5635 1493
Size: 1655 226 152 2109 1697
Fragments arranged by size:
2109 1697 1655 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4393 160
Size: 872 3361 1606
Fragments arranged by size:
3361 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5839
AvaI C'yCGr_G
Cuts at: 1870 1943 2501 2580 3164 1870
Size: 73 558 79 584 4545
Fragments arranged by size:
4545 584 558 79 73
BamHI G'GATC_C
Cuts at: 2775 3169 3536 2775
Size: 394 367 5078
Fragments arranged by size:
5078 394 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2879 2902 3160 4615 618
Size: 325 217 627 1092 23 258 1455 1842
Fragments arranged by size:
1842 1455 1092 627 325 258 217 23
BanII G_rGCy'C
Cuts at: 1391 2357 3098 3158 1391
Size: 966 741 60 4072
Fragments arranged by size:
4072 966 741 60
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2044 2422 2793 2805 2997 3028 928
Size: 971 145 378 371 12 192 31 3739
Fragments arranged by size:
3739 971 378 371 192 145 31 12
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4030 4064 1903
Size: 34 2093 34 3678
Fragments arranged by size:
3678 2093 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3731 4941 3731
Size: 1210 4629
BclI T'GATC_A
Cuts at: 1548 2991 1548
Size: 1443 4396
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2911 4450 136
Size: 107 122 71 631 605 1239 1539 1525
Fragments arranged by size:
1539 1525 1239 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5834 5834
Size: 5839
BlpI GC'TnA_GC
Cuts at: 3256 3256
Size: 5839
Bme1580I G_kGCm'C
Cuts at: 1790 2216 2537 2882 2907 3900 5056 1790
Size: 426 321 345 25 993 1156 2573
Fragments arranged by size:
2573 1156 993 426 345 321 25
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5839
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3570 4528 442
Size: 416 53 83 399 24 2153 958 1753
Fragments arranged by size:
2153 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5839
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2219 2341 4499 1239
Size: 980 122 2158 2579
Fragments arranged by size:
2579 2158 980 122
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2161 2237 2588 2677 3964 4829 5127 2161
Size: 76 351 89 1287 865 298 2873
Fragments arranged by size:
2873 1287 865 351 298 89 76
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4502 882
Size: 617 3003 2219
Fragments arranged by size:
3003 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3630 493
Size: 711 2426 2702
Fragments arranged by size:
2702 2426 711
BsaBI GATnn'nnATC
Cuts at: 5833 5833
Size: 5839
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2894 3646 4028 4663
Size: 53 83 186 936 1361 752 382 635
Cuts at: 4663 275
Size: 1451
Fragments arranged by size:
1451 1361 936 752 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 2142 4272 4670 4944 5091 5502 2142
Size: 2130 398 274 147 411 2479
Fragments arranged by size:
2479 2130 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2192 2222 1178
Size: 30 165 30 789 30 4795
Fragments arranged by size:
4795 789 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2102 2105 2665 2876 1186
Size: 916 3 560 211 4149
Fragments arranged by size:
4149 916 560 211 3
BseYI C'CCAG_C
Cuts at: 2725 5042 5538 2725
Size: 2317 496 3026
Fragments arranged by size:
3026 2317 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2810 1163
Size: 573 1074 4192
Fragments arranged by size:
4192 1074 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3289 4050 5078 1540
Size: 275 186 1288 761 1028 2301
Fragments arranged by size:
2301 1288 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2816 2961 3900 3985 5056 2816
Size: 145 939 85 1071 3599
Fragments arranged by size:
3599 1071 939 145 85
BsmI GAATG_Cn'
Cuts at: 2225 3350 3443 2225
Size: 1125 93 4621
Fragments arranged by size:
4621 1125 93
BsmBI CGTCTCn'nnnn_
Cuts at: 2885 2885
Size: 5839
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2216 2357 2537 2816 2882 2907 2961
Size: 399 426 141 180 279 66 25 54
Cuts at: 2961 3098 3158 3900 3985 5056 1391
Size: 137 60 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 426 399 279 180 141
137 85 66 60 54 25
BspEI T'CCGG_A
Cuts at: 2142 5502 2142
Size: 3360 2479
BspHI T'CATG_A
Cuts at: 3728 5458 3728
Size: 1730 4109
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2563 3194 844
Size: 1719 631 3489
Fragments arranged by size:
3489 1719 631
BsrBI CCG'CTC
Cuts at: 1394 1823 3000 3726 1394
Size: 429 1177 726 3507
Fragments arranged by size:
3507 1177 726 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4334 4508 65
Size: 1183 3086 174 1396
Fragments arranged by size:
3086 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4483 4670 1700
Size: 110 2673 187 2869
Fragments arranged by size:
2869 2673 187 110
BsrGI T'GTAC_A
Cuts at: 96 2687 96
Size: 2591 3248
BssHII G'CGCG_C
Cuts at: 1925 2242 1925
Size: 317 5522
BssSI C'ACGA_G
Cuts at: 2927 3899 4911 2927
Size: 972 1012 3855
Fragments arranged by size:
3855 1012 972
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2220 794
Size: 1426 4413
BstBI TT'CG_AA
Cuts at: 4643 4643
Size: 5839
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5839
BstXI CCAn_nnnn'nTGG
Cuts at: 2365 2365
Size: 5839
BstYI r'GATC_y
Cuts at: 1972 2775 3169 3536 3921 3938 5390 5476 5488
Size: 803 394 367 385 17 1452 86 12
Cuts at: 5488 5834 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 803 394 385 367 346 86
17 12
Bsu36I CC'TnA_GG
Cuts at: 2074 2074
Size: 5839
BtgI C'CryG_G
Cuts at: 513 1065 2004 2898 513
Size: 552 939 894 3454
Fragments arranged by size:
3454 939 894 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5100
Fragments arranged by size:
5100 683 56
BtsI GCAGTG_nn'
Cuts at: 2720 3355 4149 4169 2720
Size: 635 794 20 4390
Fragments arranged by size:
4390 794 635 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5839
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5804
DraI TTT'AAA
Cuts at: 3143 3143
Size: 5839
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4846 809
Size: 507 3530 1802
Fragments arranged by size:
3530 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2367 3018 3286
Size: 54 1425 50 275 18 537 651 268
Cuts at: 3286 4175 5804 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 889 651 537 275 268 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3286 1537
Size: 275 1474 4090
Fragments arranged by size:
4090 1474 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2108 2144 3769 1353
Size: 755 36 1625 3423
Fragments arranged by size:
3423 1625 755 36
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4426 4800 4946 387
Size: 1064 138 2837 374 146 1280
Fragments arranged by size:
2837 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2045 2219 2341 2817 3902 4499 5286
Size: 393 413 174 122 476 1085 597 787
Cuts at: 5286 5554 1239
Size: 268 1524
Fragments arranged by size:
1524 1085 787 597 476 413 393 268
174 122
EcoO109I rG'GnC_Cy
Cuts at: 2362 2362
Size: 5839
EcoRI G'AATT_C
Cuts at: 3148 3526 3148
Size: 378 5461
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2155 2869 2155
Size: 714 5125
FspI TGC'GCA
Cuts at: 4345 4345
Size: 5839
HaeII r_GCGC'y
Cuts at: 1678 4986 1678
Size: 3308 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2192 2224 2798 2830 3080
Size: 32 135 32 620 32 574 32 250
Cuts at: 3080 3112 4480 4512 4554 4586 1373
Size: 32 1368 32 42 32 2626
Fragments arranged by size:
2626 1368 620 574 250 135 42 32
32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3183 3429 669
Size: 988 281 1245 246 3079
Fragments arranged by size:
3079 1245 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5839
HpaI GTT'AAC
Cuts at: 3429 3429
Size: 5839
KpnI G_GTAC'C
Cuts at: 3164 3164
Size: 5839
MfeI C'AATT_G
Cuts at: 3438 3438
Size: 5839
MluI A'CGCG_T
Cuts at: 5715 5715
Size: 5839
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2741 4953 5137 1305
Size: 1436 2212 184 2007
Fragments arranged by size:
2212 2007 1436 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2369 3020 10
Size: 54 1425 343 537 651 2829
Fragments arranged by size:
2829 1425 651 537 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2423 2573 2645 3797 4156 4315 518
Size: 648 1257 150 72 1152 359 159 2042
Fragments arranged by size:
2042 1257 1152 648 359 159 150 72
MspA1I CmG'CkG
Cuts at: 1606 2185 2675 3255 3932 5080 5325 1606
Size: 579 490 580 677 1148 245 2120
Fragments arranged by size:
2120 1148 677 580 579 490 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 2898 513
Size: 552 939 894 3454
Fragments arranged by size:
3454 939 894 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5839
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5839
NotI GC'GGCC_GC
Cuts at: 3286 3286
Size: 5839
NspI r_CATG'y
Cuts at: 1132 1229 2572 3197 4742 1132
Size: 97 1343 625 1545 2229
Fragments arranged by size:
2229 1545 1343 625 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5839
PciI A'CATG_T
Cuts at: 4738 4738
Size: 5839
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2980 1108
Size: 1872 3967
PfoI T'CCnGG_A
Cuts at: 2490 2490
Size: 5839
PmeI GTTT'AAAC
Cuts at: 3143 3143
Size: 5839
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3908 3940 5421 5453 3908
Size: 32 1481 32 4294
Fragments arranged by size:
4294 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2362 2362
Size: 5839
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5839
PsiI TTA'TAA
Cuts at: 3409 3409
Size: 5839
PstI C_TGCA'G
Cuts at: 830 2159 3191 830
Size: 1329 1032 3478
Fragments arranged by size:
3478 1329 1032
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5839
PvuII CAG'CTG
Cuts at: 2185 2675 2185
Size: 490 5349
SalI G'TCGA_C
Cuts at: 1936 3181 1936
Size: 1245 4594
SapI GCTCTTCn'nnn_
Cuts at: 2108 2144 2108
Size: 36 5803
SbfI CC_TGCA'GG
Cuts at: 3191 3191
Size: 5839
ScaI AGT'ACT
Cuts at: 2867 4087 5799 2867
Size: 1220 1712 2907
Fragments arranged by size:
2907 1712 1220
SexAI A'CCwGG_T
Cuts at: 2046 2046
Size: 5839
SfcI C'TryA_G
Cuts at: 826 1045 2155 2869 3187 4322 5003 5194 826
Size: 219 1110 714 318 1135 681 191 1471
Fragments arranged by size:
1471 1135 1110 714 681 318 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5839
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5839
SmaI CCC'GGG
Cuts at: 1872 2582 3166 1872
Size: 710 584 4545
Fragments arranged by size:
4545 710 584
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2176 2252 2603 2656 3943 4844
Size: 197 926 233 76 351 53 1287 901
Cuts at: 4844 5106 820
Size: 262 1553
Fragments arranged by size:
1553 1287 926 901 351 262 233 197
76 53
SnaBI TAC'GTA
Cuts at: 493 3630 493
Size: 3137 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5839
SphI G_CATG'C
Cuts at: 1132 2572 3197 1132
Size: 1440 625 3774
Fragments arranged by size:
3774 1440 625
SrfI GCCC'GGGC
Cuts at: 2582 2582
Size: 5839
SspI AAT'ATT
Cuts at: 5 52 3763 5
Size: 47 3711 2081
Fragments arranged by size:
3711 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5839
StyI C'CwwG_G
Cuts at: 513 1065 2004 2358 2586 2628 2898 3278 513
Size: 552 939 354 228 42 270 380 3074
Fragments arranged by size:
3074 939 552 380 354 270 228 42
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3866 3883 4036 4221 4666 325
Size: 1255 2286 17 153 185 445 1498
Fragments arranged by size:
2286 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2687 2865 4085
Size: 274 80 33 51 1443 710 178 1220
Cuts at: 4085 5797 96
Size: 1712 138
Fragments arranged by size:
1712 1443 1220 710 274 178 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3058 3090 5799 5831 3058
Size: 32 2709 32 3066
Fragments arranged by size:
3066 2709 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5839
XbaI T'CTAG_A
Cuts at: 3175 3175
Size: 5839
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2377 2400 2377
Size: 23 5816
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5839
XmaI C'CCGG_G
Cuts at: 1870 2580 3164 1870
Size: 710 584 4545
Fragments arranged by size:
4545 710 584
XmnI GAAnn'nnTTC
Cuts at: 3968 3968
Size: 5839
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3647 276
Size: 53 83 186 936 2113 2468
Fragments arranged by size:
2468 2113 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI
BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI
EarI EciI Eco57MI EcoO109I EcoRI FalI FspI HaeII
Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI
MscI MslI MspA1I NcoI NdeI NheI NotI NspI
PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI
PsiI PstI PvuI PvuII SalI SapI SbfI ScaI
SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SrfI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI
BfrBI BplI Bpu10I BsiWI BstZ17I DraIII EcoICRI EcoNI
EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI
NsiI PacI PmlI PspOMI PspXI PsrI RsrII SacI
SacII SanDI SfiI SfoI SwaI