Restriction map for
pFN21AB5411
(Circular) (Six-base) MAPSORT of: pFN21AB5411.seq Check: 8984 from: 1 to: 6490
pFN21AB5411 6490 bp
With 182 enzymes: SgfI *
August 16, 2010 14:41 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4300 278
Size: 53 83 186 936 2764 2468
Fragments arranged by size:
2764 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3833 1937
Size: 1896 4594
Acc65I G'GTAC_C
Cuts at: 3811 3811
Size: 6490
AclI AA'CG_TT
Cuts at: 1563 4617 4990 1563
Size: 3054 373 3063
Fragments arranged by size:
3063 3054 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2020 2340 2648 3338 3386 4553 5937 6205
Size: 388 320 308 690 48 1167 1384 268
Cuts at: 6205 1632
Size: 1917
Fragments arranged by size:
1917 1384 1167 690 388 320 308 268
48
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6293
AflIII A'CryG_T
Cuts at: 1203 5389 6366 1203
Size: 4186 977 1327
Fragments arranged by size:
4186 1327 977
AgeI A'CCGG_T
Cuts at: 5321 5321
Size: 6490
AhdI GACnn_n'nnGTC
Cuts at: 3517 5219 3517
Size: 1702 4788
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6490
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2714 3623 1184
Size: 1530 909 4051
Fragments arranged by size:
4051 1530 909
AlwNI CAG_nnn'CTG
Cuts at: 2644 3218 3376 4233 5805 2644
Size: 574 158 857 1572 3329
Fragments arranged by size:
3329 1572 857 574 158
ApaLI G'TGCA_C
Cuts at: 4547 5703 4547
Size: 1156 5334
ApoI r'AATT_y
Cuts at: 1493 2300 3799 4025 4177 6286 1493
Size: 807 1499 226 152 2109 1697
Fragments arranged by size:
2109 1697 1499 807 226 152
AseI AT'TA_AT
Cuts at: 160 1032 5044 160
Size: 872 4012 1606
Fragments arranged by size:
4012 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6490
AvaI C'yCGr_G
Cuts at: 1870 1943 3024 3765 3815 1870
Size: 73 1081 741 50 4545
Fragments arranged by size:
4545 1081 741 73 50
BamHI G'GATC_C
Cuts at: 3820 4187 3820
Size: 367 6123
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3811 5266 618
Size: 325 217 627 2024 1455 1842
Fragments arranged by size:
2024 1842 1455 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2172 3789 3809 1391
Size: 781 1617 20 4072
Fragments arranged by size:
4072 1617 781 20
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2842 928
Size: 971 943 4576
Fragments arranged by size:
4576 971 943
BbvCI CC'TCA_GC
Cuts at: 3327 3327
Size: 6490
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4681 4715 1903
Size: 34 2744 34 3678
Fragments arranged by size:
3678 2744 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4382 5592 4382
Size: 1210 5280
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6490
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2444 3206 3215
Size: 107 122 71 631 605 772 762 9
Cuts at: 3215 3353 5101 136
Size: 138 1748 1525
Fragments arranged by size:
1748 1525 772 762 631 605 138 122
107 71 9
BglII A'GATC_T
Cuts at: 2394 2763 6485 2394
Size: 369 3722 2399
Fragments arranged by size:
3722 2399 369
BlpI GC'TnA_GC
Cuts at: 3219 3907 3219
Size: 688 5802
Bme1580I G_kGCm'C
Cuts at: 1790 2693 4551 5707 1790
Size: 903 1858 1156 2573
Fragments arranged by size:
2573 1858 1156 903
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6490
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2463 4221 5179
Size: 416 53 83 399 24 1046 1758 958
Cuts at: 5179 442
Size: 1753
Fragments arranged by size:
1758 1753 1046 958 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6490
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2666 2879 3443 5150 1239
Size: 1427 213 564 1707 2579
Fragments arranged by size:
2579 1707 1427 564 213
Bpu10I CC'TnA_GC
Cuts at: 2637 3327 2637
Size: 690 5800
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2030 3614 4615 5480 5778 2030
Size: 1584 1001 865 298 2742
Fragments arranged by size:
2742 1584 1001 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 5153 882
Size: 617 3654 2219
Fragments arranged by size:
3654 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3444 4281 493
Size: 711 2240 837 2702
Fragments arranged by size:
2702 2240 837 711
BsaBI GATnn'nnATC
Cuts at: 6484 6484
Size: 6490
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2094 4297 4679 5314
Size: 53 83 186 936 561 2203 382 635
Cuts at: 5314 275
Size: 1451
Fragments arranged by size:
2203 1451 936 635 561 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 2545 2676 4923 5321 5595 5742 6153 2545
Size: 131 2247 398 274 147 411 2882
Fragments arranged by size:
2882 2247 411 398 274 147 131
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 6265
Fragments arranged by size:
6265 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2339 2573 2854 2944 2999 3059 3091 3152
Size: 1153 234 281 90 55 60 32 61
Cuts at: 3152 3194 3197 3278 3281 3344 3355 1186
Size: 42 3 81 3 63 11 4321
Fragments arranged by size:
4321 1153 281 234 90 81 63 61
60 55 42 32 11 3 3
BseYI C'CCAG_C
Cuts at: 5693 6189 5693
Size: 496 5994
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 3671 1163
Size: 573 1935 3982
Fragments arranged by size:
3982 1935 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3940 4701 5729 1540
Size: 275 186 1939 761 1028 2301
Fragments arranged by size:
2301 1939 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2424 2589 3789 4551 4636 5707 2424
Size: 165 1200 762 85 1071 3207
Fragments arranged by size:
3207 1200 1071 762 165 85
BsmI GAATG_Cn'
Cuts at: 2320 4001 4094 2320
Size: 1681 93 4716
Fragments arranged by size:
4716 1681 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2172 2424 2589 2693 3789 3809 4551
Size: 399 382 252 165 104 1096 20 742
Cuts at: 4551 4636 5707 1391
Size: 85 1071 2174
Fragments arranged by size:
2174 1096 1071 742 399 382 252 165
104 85 20
BspEI T'CCGG_A
Cuts at: 2545 2676 6153 2545
Size: 131 3477 2882
Fragments arranged by size:
3477 2882 131
BspHI T'CATG_A
Cuts at: 2254 4379 6109 2254
Size: 2125 1730 2635
Fragments arranged by size:
2635 2125 1730
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3384 3845 844
Size: 2540 461 3489
Fragments arranged by size:
3489 2540 461
BsrBI CCG'CTC
Cuts at: 1394 1823 3010 4377 1394
Size: 429 1187 1367 3507
Fragments arranged by size:
3507 1367 1187 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4985 5159 65
Size: 1183 3737 174 1396
Fragments arranged by size:
3737 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 5134 5321 1700
Size: 110 3324 187 2869
Fragments arranged by size:
3324 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6490
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6490
BssSI C'ACGA_G
Cuts at: 3058 4550 5562 3058
Size: 1492 1012 3986
Fragments arranged by size:
3986 1492 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 3229 3292 3376 794
Size: 2435 63 84 3908
Fragments arranged by size:
3908 2435 84 63
BstBI TT'CG_AA
Cuts at: 5294 5294
Size: 6490
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6490
BstYI r'GATC_y
Cuts at: 1972 2394 2763 3820 4187 4572 4589 6041 6127
Size: 422 369 1057 367 385 17 1452 86
Cuts at: 6127 6139 6485 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 1057 422 385 369 367 346
86 17 12
Bsu36I CC'TnA_GG
Cuts at: 2409 2409
Size: 6490
BtgI C'CryG_G
Cuts at: 513 1065 2004 2088 2774 3558 513
Size: 552 939 84 686 784 3445
Fragments arranged by size:
3445 939 784 686 552 84
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2002 487
Size: 683 56 776 4975
Fragments arranged by size:
4975 776 683 56
BtsI GCAGTG_nn'
Cuts at: 4006 4800 4820 4006
Size: 794 20 5676
Fragments arranged by size:
5676 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6490
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6455
DraI TTT'AAA
Cuts at: 3794 3794
Size: 6490
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5497 809
Size: 507 4181 1802
Fragments arranged by size:
4181 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2007 2295 3111
Size: 54 1425 50 275 18 177 288 816
Cuts at: 3111 3937 4826 6455 8
Size: 826 889 1629 43
Fragments arranged by size:
1629 1425 889 826 816 288 275 177
54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3937 1537
Size: 275 2125 4090
Fragments arranged by size:
4090 2125 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2316 3397 3777 4420 1353
Size: 963 1081 380 643 3423
Fragments arranged by size:
3423 1081 963 643 380
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3410 5077 5451 5597 387
Size: 1064 138 1821 1667 374 146 1280
Fragments arranged by size:
1821 1667 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 3787 3787
Size: 6490
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2020 2340 2648 2666 2879 3338 3386
Size: 393 388 320 308 18 213 459 48
Cuts at: 3386 3443 4553 5150 5937 6205 1239
Size: 57 1110 597 787 268 1524
Fragments arranged by size:
1524 1110 787 597 459 393 388 320
308 268 213 57 48 18
EcoO109I rG'GnC_Cy
Cuts at: 2137 2641 3373 2137
Size: 504 732 5254
Fragments arranged by size:
5254 732 504
EcoRI G'AATT_C
Cuts at: 3799 4177 3799
Size: 378 6112
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2158 2785 3763 2158
Size: 627 978 4885
Fragments arranged by size:
4885 978 627
FspI TGC'GCA
Cuts at: 4996 4996
Size: 6490
HaeII r_GCGC'y
Cuts at: 1678 3019 3277 3759 5637 1678
Size: 1341 258 482 1878 2531
Fragments arranged by size:
2531 1878 1341 482 258
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 3503 3535 3589 3621 5131
Size: 32 135 32 1931 32 54 32 1510
Cuts at: 5131 5163 5205 5237 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1931 1510 135 54 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3834 4080 669
Size: 988 281 1896 246 3079
Fragments arranged by size:
3079 1896 988 281 246
HindIII A'AGCT_T
Cuts at: 748 2870 3493 748
Size: 2122 623 3745
Fragments arranged by size:
3745 2122 623
HpaI GTT'AAC
Cuts at: 4080 4080
Size: 6490
KpnI G_GTAC'C
Cuts at: 3815 3815
Size: 6490
MfeI C'AATT_G
Cuts at: 2110 4089 2110
Size: 1979 4511
MluI A'CGCG_T
Cuts at: 6366 6366
Size: 6490
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3051 3726 5604 5788 1305
Size: 1746 675 1878 184 2007
Fragments arranged by size:
2007 1878 1746 675 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2297 3113 10
Size: 54 1425 343 465 816 3387
Fragments arranged by size:
3387 1425 816 465 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2733 4448 4807 4966 518
Size: 648 1567 1715 359 159 2042
Fragments arranged by size:
2042 1715 1567 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 3146 3364 3906 4583 5731 5976 1606
Size: 1540 218 542 677 1148 245 2120
Fragments arranged by size:
2120 1540 1148 677 542 245 218
NcoI C'CATG_G
Cuts at: 513 1065 2004 2088 3558 513
Size: 552 939 84 1470 3445
Fragments arranged by size:
3445 1470 939 552 84
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6490
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6490
NotI GC'GGCC_GC
Cuts at: 3937 3937
Size: 6490
NspI r_CATG'y
Cuts at: 1132 1229 3848 5393 1132
Size: 97 2619 1545 2229
Fragments arranged by size:
2619 2229 1545 97
PasI CC'CwG_GG
Cuts at: 1348 2719 1348
Size: 1371 5119
PciI A'CATG_T
Cuts at: 5389 5389
Size: 6490
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6490
PfoI T'CCnGG_A
Cuts at: 3420 3420
Size: 6490
PmeI GTTT'AAAC
Cuts at: 3794 3794
Size: 6490
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2090 2122 4559 4591 6072 6104 2090
Size: 32 2437 32 1481 32 2476
Fragments arranged by size:
2476 2437 1481 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 2137 2641 3373 2137
Size: 504 732 5254
Fragments arranged by size:
5254 732 504
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6490
PsiI TTA'TAA
Cuts at: 4060 4060
Size: 6490
PstI C_TGCA'G
Cuts at: 830 2199 3090 3381 3612 3842 830
Size: 1369 891 291 231 230 3478
Fragments arranged by size:
3478 1369 891 291 231 230
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6490
PvuII CAG'CTG
Cuts at: 3146 3364 3146
Size: 218 6272
SacI G_AGCT'C
Cuts at: 3789 3789
Size: 6490
SalI G'TCGA_C
Cuts at: 1936 3832 1936
Size: 1896 4594
SapI GCTCTTCn'nnn_
Cuts at: 3397 3777 3397
Size: 380 6110
SbfI CC_TGCA'GG
Cuts at: 3381 3842 3381
Size: 461 6029
ScaI AGT'ACT
Cuts at: 4738 6450 4738
Size: 1712 4778
SexAI A'CCwGG_T
Cuts at: 2180 2180
Size: 6490
SfcI C'TryA_G
Cuts at: 826 1045 2195 3086 3377 3608 3838 4973 5654
Size: 219 1150 891 291 231 230 1135 681
Cuts at: 5654 5845 826
Size: 191 1471
Fragments arranged by size:
1471 1150 1135 891 681 291 231 230
219 191
SfiI GGCCn_nnn'nGGCC
Cuts at: 3206 3206
Size: 6490
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6490
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6490
SmaI CCC'GGG
Cuts at: 1872 3817 1872
Size: 1945 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2045 3024 3629 4594 5495 5757
Size: 197 926 102 979 605 965 901 262
Cuts at: 5757 820
Size: 1553
Fragments arranged by size:
1553 979 965 926 901 605 262 197
102
SnaBI TAC'GTA
Cuts at: 493 4281 493
Size: 3788 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6490
SphI G_CATG'C
Cuts at: 1132 3848 1132
Size: 2716 3774
SspI AAT'ATT
Cuts at: 5 52 4414 5
Size: 47 4362 2081
Fragments arranged by size:
4362 2081 47
StuI AGG'CCT
Cuts at: 1517 3647 1517
Size: 2130 4360
StyI C'CwwG_G
Cuts at: 513 1065 2004 2088 3558 3929 513
Size: 552 939 84 1470 371 3074
Fragments arranged by size:
3074 1470 939 552 371 84
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 4517 4534 4687 4872 5317 325
Size: 1255 2937 17 153 185 445 1498
Fragments arranged by size:
2937 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2199 4736 6448
Size: 274 80 33 51 1443 222 2537 1712
Cuts at: 6448 96
Size: 138
Fragments arranged by size:
2537 1712 1443 274 222 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6450 6482 6450
Size: 32 6458
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6490
XbaI T'CTAG_A
Cuts at: 3826 3826
Size: 6490
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2450 3766 2450
Size: 1316 5174
XhoI C'TCGA_G
Cuts at: 1943 3024 1943
Size: 1081 5409
XmaI C'CCGG_G
Cuts at: 1870 3815 1870
Size: 1945 4545
XmnI GAAnn'nnTTC
Cuts at: 2508 4619 2508
Size: 2111 4379
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4298 276
Size: 53 83 186 936 2764 2468
Fragments arranged by size:
2764 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI
BtsI ClaI CspCI DraI DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII
Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI
MscI MslI MspA1I NcoI NdeI NheI NotI NspI
PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI
PsiI PstI PvuI PvuII SacI SalI SapI SbfI
ScaI SexAI SfcI SfiI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BfrBI BplI BsiWI BsmBI BstXI BstZ17I DraIII EcoNI
EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI
NsiI PacI PmlI PspOMI PspXI PsrI RsrII SacII
SanDI SfoI SrfI SwaI