Restriction map for
pFN21AB5407
(Circular) (Six-base) MAPSORT of: pFN21AB5407.seq Check: 5503 from: 1 to: 6187
pFN21AB5407 6187 bp
With 182 enzymes: SgfI *
April 15, 2010 09:30 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2095 2617 2095
Size: 522 5665
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3997 278
Size: 53 83 186 936 2461 2468
Fragments arranged by size:
2468 2461 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3530 1937
Size: 1593 4594
Acc65I G'GTAC_C
Cuts at: 3508 3508
Size: 6187
AclI AA'CG_TT
Cuts at: 1563 4314 4687 1563
Size: 2751 373 3063
Fragments arranged by size:
3063 2751 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3428 4250 5634 5902 1632
Size: 1796 822 1384 268 1917
Fragments arranged by size:
1917 1796 1384 822 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5990
AflIII A'CryG_T
Cuts at: 1203 5086 6063 1203
Size: 3883 977 1327
Fragments arranged by size:
3883 1327 977
AgeI A'CCGG_T
Cuts at: 5018 5018
Size: 6187
AhdI GACnn_n'nnGTC
Cuts at: 4916 4916
Size: 6187
AleI CACnn'nnGTG
Cuts at: 1166 2829 1166
Size: 1663 4524
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2847 1184
Size: 1663 4524
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2351 2383 2351
Size: 32 6155
AlwNI CAG_nnn'CTG
Cuts at: 2110 2632 2878 3371 3930 5502 2110
Size: 522 246 493 559 1572 2795
Fragments arranged by size:
2795 1572 559 522 493 246
ApaLI G'TGCA_C
Cuts at: 4244 5400 4244
Size: 1156 5031
ApoI r'AATT_y
Cuts at: 1493 3496 3722 3874 5983 1493
Size: 2003 226 152 2109 1697
Fragments arranged by size:
2109 2003 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4741 160
Size: 872 3709 1606
Fragments arranged by size:
3709 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6187
AvaI C'yCGr_G
Cuts at: 1870 1943 2528 2618 2926 3512 1870
Size: 73 585 90 308 586 4545
Fragments arranged by size:
4545 586 585 308 90 73
BamHI G'GATC_C
Cuts at: 3517 3884 3517
Size: 367 5820
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2487 2609 2831 3385 3508
Size: 325 217 627 700 122 222 554 123
Cuts at: 3508 4963 618
Size: 1455 1842
Fragments arranged by size:
1842 1455 700 627 554 325 222 217
123 122
BanII G_rGCy'C
Cuts at: 1391 2054 2160 3338 3506 1391
Size: 663 106 1178 168 4072
Fragments arranged by size:
4072 1178 663 168 106
BbeI G_GCGC'C
Cuts at: 2491 3389 2491
Size: 898 5289
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3007 928
Size: 971 1108 4108
Fragments arranged by size:
4108 1108 971
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4378 4412 1903
Size: 34 2441 34 3678
Fragments arranged by size:
3678 2441 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4079 5289 4079
Size: 1210 4977
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6187
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2873 4798 136
Size: 107 122 71 631 605 1201 1925 1525
Fragments arranged by size:
1925 1525 1201 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6182 6182
Size: 6187
BlpI GC'TnA_GC
Cuts at: 3604 3604
Size: 6187
Bme1580I G_kGCm'C
Cuts at: 1790 2543 2612 2836 4248 5404 1790
Size: 753 69 224 1412 1156 2573
Fragments arranged by size:
2573 1412 1156 753 224 69
BmgBI CAC'GTC
Cuts at: 1318 2782 1318
Size: 1464 4723
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2984 3073 3918
Size: 416 53 83 399 24 1567 89 845
Cuts at: 3918 4876 442
Size: 958 1753
Fragments arranged by size:
1753 1567 958 845 416 399 89 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6187
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2438 2540 2690 2729 3281 3289 3305 3348
Size: 1199 102 150 39 552 8 16 43
Cuts at: 3348 3458 4847 1239
Size: 110 1389 2579
Fragments arranged by size:
2579 1389 1199 552 150 110 102 43
39 16 8
Bpu10I CC'TnA_GC
Cuts at: 2396 2396
Size: 6187
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2348 2939 4312 5177 5475 2348
Size: 591 1373 865 298 3060
Fragments arranged by size:
3060 1373 865 591 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2135 3050 3257 4850 882
Size: 617 636 915 207 1593 2219
Fragments arranged by size:
2219 1593 915 636 617 207
BsaAI yAC'GTr
Cuts at: 493 1204 2806 3978 493
Size: 711 1602 1172 2702
Fragments arranged by size:
2702 1602 1172 711
BsaBI GATnn'nnATC
Cuts at: 6181 6181
Size: 6187
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2256 2488 2637 3386
Size: 53 83 186 936 723 232 149 749
Cuts at: 3386 3994 4376 5011 275
Size: 608 382 635 1451
Fragments arranged by size:
1451 936 749 723 635 608 382 232
186 149 83 53
BsaWI w'CCGG_w
Cuts at: 4620 5018 5292 5439 5850 4620
Size: 398 274 147 411 4957
Fragments arranged by size:
4957 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2672 2702 1178
Size: 30 165 30 1269 30 4663
Fragments arranged by size:
4663 1269 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2114 2426 2435 2914 3464 1186
Size: 928 312 9 479 550 3909
Fragments arranged by size:
3909 928 550 479 312 9
BseYI C'CCAG_C
Cuts at: 2174 3148 5390 5886 2174
Size: 974 2242 496 2475
Fragments arranged by size:
2475 2242 974 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2459 2732 2879 2891 1163
Size: 573 723 273 147 12 4459
Fragments arranged by size:
4459 723 573 273 147 12
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2475 3637 4398 5426 1540
Size: 275 186 474 1162 761 1028 2301
Fragments arranged by size:
2301 1162 1028 761 474 275 186
BsiHKAI G_wGCw'C
Cuts at: 2054 2160 2565 2661 4248 4333 5404 2054
Size: 106 405 96 1587 85 1071 2837
Fragments arranged by size:
2837 1587 1071 405 106 96 85
BsmI GAATG_Cn'
Cuts at: 3698 3791 3698
Size: 93 6094
BsmBI CGTCTCn'nnnn_
Cuts at: 2590 2675 2590
Size: 85 6102
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2054 2160 2543 2565 2612 2661 2836
Size: 399 264 106 383 22 47 49 175
Cuts at: 2836 3338 3506 4248 4333 5404 1391
Size: 502 168 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 502 399 383 264 175
168 106 85 49 47 22
BspEI T'CCGG_A
Cuts at: 5850 5850
Size: 6187
BspHI T'CATG_A
Cuts at: 2766 4076 5806 2766
Size: 1310 1730 3147
Fragments arranged by size:
3147 1730 1310
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2095 2617 2724 3542 844
Size: 1251 522 107 818 3489
Fragments arranged by size:
3489 1251 818 522 107
BsrBI CCG'CTC
Cuts at: 1394 1823 4074 1394
Size: 429 2251 3507
Fragments arranged by size:
3507 2251 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4682 4856 65
Size: 1183 3434 174 1396
Fragments arranged by size:
3434 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 3402 4831 5018 1700
Size: 110 1592 1429 187 2869
Fragments arranged by size:
2869 1592 1429 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6187
BssHII G'CGCG_C
Cuts at: 1925 2429 2897 1925
Size: 504 468 5215
Fragments arranged by size:
5215 504 468
BssSI C'ACGA_G
Cuts at: 3278 4247 5259 3278
Size: 969 1012 4206
Fragments arranged by size:
4206 1012 969
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2869 2878 794
Size: 2075 9 4103
Fragments arranged by size:
4103 2075 9
BstBI TT'CG_AA
Cuts at: 4991 4991
Size: 6187
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6187
BstYI r'GATC_y
Cuts at: 1972 3517 3884 4269 4286 5738 5824 5836 6182
Size: 1545 367 385 17 1452 86 12 346
Cuts at: 6182 1972
Size: 1977
Fragments arranged by size:
1977 1545 1452 385 367 346 86 17
12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2592 3036 3267 513
Size: 552 939 588 444 231 3433
Fragments arranged by size:
3433 939 588 552 444 231
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2878 3175 3181 487
Size: 683 56 1652 297 6 3493
Fragments arranged by size:
3493 1652 683 297 56 6
BtsI GCAGTG_nn'
Cuts at: 3006 3349 3703 4497 4517 3006
Size: 343 354 794 20 4676
Fragments arranged by size:
4676 794 354 343 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6187
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6152
DraI TTT'AAA
Cuts at: 3491 3491
Size: 6187
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5194 809
Size: 507 3878 1802
Fragments arranged by size:
3878 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2472 2556 2787
Size: 54 1425 50 275 18 642 84 231
Cuts at: 2787 3165 3270 3634 4523 6152 8
Size: 378 105 364 889 1629 43
Fragments arranged by size:
1629 1425 889 642 378 364 275 231
105 84 54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2472 3634 1537
Size: 275 660 1162 4090
Fragments arranged by size:
4090 1162 660 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2732 4117 1353
Size: 1379 1385 3423
Fragments arranged by size:
3423 1385 1379
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2168 4774 5148 5294 387
Size: 1064 138 579 2606 374 146 1280
Fragments arranged by size:
2606 1280 1064 579 374 146 138
EcoICRI GAG'CTC
Cuts at: 2052 2158 2052
Size: 106 6081
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2438 2540 2690 2729 3281 3289 3305
Size: 393 806 102 150 39 552 8 16
Cuts at: 3305 3348 3428 3458 4250 4847 5634 5902 1239
Size: 43 80 30 792 597 787 268 1524
Fragments arranged by size:
1524 806 792 787 597 552 393 268
150 102 80 43 39 30 16 8
EcoNI CCTnn'n_nnAGG
Cuts at: 3446 3446
Size: 6187
EcoO109I rG'GnC_Cy
Cuts at: 2119 2248 3465 2119
Size: 129 1217 4841
Fragments arranged by size:
4841 1217 129
EcoRI G'AATT_C
Cuts at: 3496 3874 3496
Size: 378 5809
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2767 2980 2767
Size: 213 5974
FspI TGC'GCA
Cuts at: 2891 4693 2891
Size: 1802 4385
FspAI rTGC'GCAy
Cuts at: 2891 2891
Size: 6187
HaeII r_GCGC'y
Cuts at: 1678 2491 2879 3389 5334 1678
Size: 813 388 510 1945 2531
Fragments arranged by size:
2531 1945 813 510 388
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 4828 4860 4902 4934 1373
Size: 32 135 32 3256 32 42 32 2626
Fragments arranged by size:
3256 2626 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2086 3531 3777 669
Size: 988 281 148 1445 246 3079
Fragments arranged by size:
3079 1445 988 281 246 148
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6187
HpaI GTT'AAC
Cuts at: 3777 3777
Size: 6187
KasI G'GCGC_C
Cuts at: 2487 3385 2487
Size: 898 5289
KpnI G_GTAC'C
Cuts at: 3512 3512
Size: 6187
MfeI C'AATT_G
Cuts at: 3786 3786
Size: 6187
MluI A'CGCG_T
Cuts at: 6063 6063
Size: 6187
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5301 5485 1305
Size: 3996 184 2007
Fragments arranged by size:
3996 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2789 10
Size: 54 1425 343 957 3408
Fragments arranged by size:
3408 1425 957 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2276 2603 2829 3104 4145 4504 4663
Size: 648 1110 327 226 275 1041 359 159
Cuts at: 4663 518
Size: 2042
Fragments arranged by size:
2042 1110 1041 648 359 327 275 226
159
MspA1I CmG'CkG
Cuts at: 1606 2057 2484 2493 3269 3312 3603 4280 5428
Size: 451 427 9 776 43 291 677 1148
Cuts at: 5428 5673 1606
Size: 245 2120
Fragments arranged by size:
2120 1148 776 677 451 427 291 245
43 9
NaeI GCC'GGC
Cuts at: 3404 3404
Size: 6187
NarI GG'CG_CC
Cuts at: 2488 3386 2488
Size: 898 5289
NcoI C'CATG_G
Cuts at: 513 1065 2004 3036 513
Size: 552 939 1032 3664
Fragments arranged by size:
3664 1032 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6187
NgoMIV G'CCGG_C
Cuts at: 3402 3402
Size: 6187
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6187
NotI GC'GGCC_GC
Cuts at: 3634 3634
Size: 6187
NspI r_CATG'y
Cuts at: 1132 1229 3545 5090 1132
Size: 97 2316 1545 2229
Fragments arranged by size:
2316 2229 1545 97
PasI CC'CwG_GG
Cuts at: 1348 3025 1348
Size: 1677 4510
PciI A'CATG_T
Cuts at: 5086 5086
Size: 6187
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6187
PfoI T'CCnGG_A
Cuts at: 2031 2370 2448 2517 3258 2031
Size: 339 78 69 741 4960
Fragments arranged by size:
4960 741 339 78 69
PmeI GTTT'AAAC
Cuts at: 3491 3491
Size: 6187
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4256 4288 5769 5801 4256
Size: 32 1481 32 4642
Fragments arranged by size:
4642 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2119 2248 3465 2119
Size: 129 1217 4841
Fragments arranged by size:
4841 1217 129
PshAI GACnn'nnGTC
Cuts at: 1613 3113 1613
Size: 1500 4687
PsiI TTA'TAA
Cuts at: 3757 3757
Size: 6187
PstI C_TGCA'G
Cuts at: 830 2628 2937 3398 3539 830
Size: 1798 309 461 141 3478
Fragments arranged by size:
3478 1798 461 309 141
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6187
SacI G_AGCT'C
Cuts at: 2054 2160 2054
Size: 106 6081
SacII CC_GC'GG
Cuts at: 3270 3270
Size: 6187
SalI G'TCGA_C
Cuts at: 1936 3529 1936
Size: 1593 4594
SanDI GG'GwC_CC
Cuts at: 2248 2248
Size: 6187
SbfI CC_TGCA'GG
Cuts at: 3539 3539
Size: 6187
ScaI AGT'ACT
Cuts at: 4435 6147 4435
Size: 1712 4475
SfcI C'TryA_G
Cuts at: 826 1045 2624 2933 3394 3535 4670 5351 5542
Size: 219 1579 309 461 141 1135 681 191
Cuts at: 5542 826
Size: 1471
Fragments arranged by size:
1579 1471 1135 681 461 309 219 191
141
SfoI GGC'GCC
Cuts at: 2489 3387 2489
Size: 898 5289
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6187
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6187
SmaI CCC'GGG
Cuts at: 1872 3514 1872
Size: 1642 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2363 2954 4291 5192 5454 820
Size: 197 926 420 591 1337 901 262 1553
Fragments arranged by size:
1553 1337 926 901 591 420 262 197
SnaBI TAC'GTA
Cuts at: 493 3978 493
Size: 3485 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6187
SphI G_CATG'C
Cuts at: 1132 3545 1132
Size: 2413 3774
SspI AAT'ATT
Cuts at: 5 52 4111 5
Size: 47 4059 2081
Fragments arranged by size:
4059 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6187
StyI C'CwwG_G
Cuts at: 513 1065 2004 3036 3626 513
Size: 552 939 1032 590 3074
Fragments arranged by size:
3074 1032 939 590 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2752 2819 3461 4214 4231 4384 4569
Size: 1255 1172 67 642 753 17 153 185
Cuts at: 4569 5014 325
Size: 445 1498
Fragments arranged by size:
1498 1255 1172 753 642 445 185 153
67 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2280 4433 6145
Size: 274 80 33 51 1443 303 2153 1712
Cuts at: 6145 96
Size: 138
Fragments arranged by size:
2153 1712 1443 303 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2672 2704 6147 6179 2672
Size: 32 3443 32 2680
Fragments arranged by size:
3443 2680 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6187
XbaI T'CTAG_A
Cuts at: 3523 3523
Size: 6187
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3145 3280 3145
Size: 135 6052
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6187
XmaI C'CCGG_G
Cuts at: 1870 3512 1870
Size: 1642 4545
XmnI GAAnn'nnTTC
Cuts at: 4316 4316
Size: 6187
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3995 276
Size: 53 83 186 936 2461 2468
Fragments arranged by size:
2468 2461 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI
BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI
FalI FspI FspAI HaeII Hin4I HincII HindIII HpaI
KasI KpnI MfeI MluI MmeI MscI MslI MspA1I
NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI
PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI
PsiI PstI PvuI SacI SacII SalI SanDI SbfI
ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI ApaI AscI AvrII BaeI BbvCI BfrBI BplI
BsiWI BstXI BstZ17I Bsu36I DraIII EcoRV FseI NruI
NsiI PacI PmlI PspOMI PspXI PsrI PvuII RsrII
SapI SexAI SfiI SrfI SwaI