Restriction map for
pFN21AB5402
(Circular) (Six-base) MAPSORT of: pFN21AB5402.seq Check: 7055 from: 1 to: 5857
pFN21AB5402 5857 bp
With 182 enzymes: SgfI *
February 27, 2009 14:45 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3667 278
Size: 53 83 186 936 2131 2468
Fragments arranged by size:
2468 2131 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2370 3200 1937
Size: 433 830 4594
Fragments arranged by size:
4594 830 433
Acc65I G'GTAC_C
Cuts at: 3178 3178
Size: 5857
AclI AA'CG_TT
Cuts at: 1563 3984 4357 1563
Size: 2421 373 3063
Fragments arranged by size:
3063 2421 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2882 3920 5304 5572 1632
Size: 1250 1038 1384 268 1917
Fragments arranged by size:
1917 1384 1250 1038 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5660
AflIII A'CryG_T
Cuts at: 1203 4756 5733 1203
Size: 3553 977 1327
Fragments arranged by size:
3553 1327 977
AgeI A'CCGG_T
Cuts at: 3091 4688 3091
Size: 1597 4260
AhdI GACnn_n'nnGTC
Cuts at: 4586 4586
Size: 5857
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5857
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2608 1184
Size: 1424 4433
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2794 2826 2794
Size: 32 5825
AlwNI CAG_nnn'CTG
Cuts at: 2299 2935 3037 3600 5172 2299
Size: 636 102 563 1572 2984
Fragments arranged by size:
2984 1572 636 563 102
ApaI G_GGCC'C
Cuts at: 2170 2170
Size: 5857
ApaLI G'TGCA_C
Cuts at: 3914 5070 3914
Size: 1156 4701
ApoI r'AATT_y
Cuts at: 1493 3166 3392 3544 5653 1493
Size: 1673 226 152 2109 1697
Fragments arranged by size:
2109 1697 1673 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4411 160
Size: 872 3379 1606
Fragments arranged by size:
3379 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5857
AvaI C'yCGr_G
Cuts at: 1870 1943 2169 3182 1870
Size: 73 226 1013 4545
Fragments arranged by size:
4545 1013 226 73
BamHI G'GATC_C
Cuts at: 2685 3187 3554 2685
Size: 502 367 4988
Fragments arranged by size:
4988 502 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2028 3056 3088 3094 3178
Size: 325 217 627 241 1028 32 6 84
Cuts at: 3178 4633 618
Size: 1455 1842
Fragments arranged by size:
1842 1455 1028 627 325 241 217 84
32 6
BanII G_rGCy'C
Cuts at: 1391 2170 2473 3044 3123 3176 1391
Size: 779 303 571 79 53 4072
Fragments arranged by size:
4072 779 571 303 79 53
BbeI G_GCGC'C
Cuts at: 2032 2032
Size: 5857
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2703 2849 928
Size: 971 804 146 3936
Fragments arranged by size:
3936 971 804 146
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4048 4082 1903
Size: 34 2111 34 3678
Fragments arranged by size:
3678 2111 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3749 4959 3749
Size: 1210 4647
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5857
BfrBI ATG'CAT
Cuts at: 2585 2585
Size: 5857
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4468 136
Size: 107 122 71 631 605 2796 1525
Fragments arranged by size:
2796 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5852 5852
Size: 5857
BlpI GC'TnA_GC
Cuts at: 3122 3274 3122
Size: 152 5705
Bme1580I G_kGCm'C
Cuts at: 1790 2170 3918 5074 1790
Size: 380 1748 1156 2573
Fragments arranged by size:
2573 1748 1156 380
BmgBI CAC'GTC
Cuts at: 1318 2016 1318
Size: 698 5159
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2534 3588 4546
Size: 416 53 83 399 24 1117 1054 958
Cuts at: 4546 442
Size: 1753
Fragments arranged by size:
1753 1117 1054 958 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5857
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 4517 1239
Size: 3278 2579
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3982 4847 5145 3982
Size: 865 298 4694
Fragments arranged by size:
4694 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2908 4520 882
Size: 617 1409 1612 2219
Fragments arranged by size:
2219 1612 1409 617
BsaAI yAC'GTr
Cuts at: 493 1204 2197 3648 493
Size: 711 993 1451 2702
Fragments arranged by size:
2702 1451 993 711
BsaBI GATnn'nnATC
Cuts at: 5851 5851
Size: 5857
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2029 2250 3664 4046
Size: 53 83 186 936 496 221 1414 382
Cuts at: 4046 4681 275
Size: 635 1451
Fragments arranged by size:
1451 1414 936 635 496 382 221 186
83 53
BsaWI w'CCGG_w
Cuts at: 3091 4290 4688 4962 5109 5520 3091
Size: 1199 398 274 147 411 3428
Fragments arranged by size:
3428 1199 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2705 2735 2763 2793 1178
Size: 30 165 30 1302 30 28 30 4242
Fragments arranged by size:
4242 1302 165 30 30 30 30 28
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2321 2905 2908 1186
Size: 1135 584 3 4135
Fragments arranged by size:
4135 1135 584 3
BseYI C'CCAG_C
Cuts at: 2798 2976 5060 5556 2798
Size: 178 2084 496 3099
Fragments arranged by size:
3099 2084 496 178
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2597 3049 1163
Size: 573 861 452 3971
Fragments arranged by size:
3971 861 573 452
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2259 3307 4068 5096 1540
Size: 275 186 258 1048 761 1028 2301
Fragments arranged by size:
2301 1048 1028 761 275 258 186
BsiHKAI G_wGCw'C
Cuts at: 2082 3123 3918 4003 5074 2082
Size: 1041 795 85 1071 2865
Fragments arranged by size:
2865 1071 1041 795 85
BsmI GAATG_Cn'
Cuts at: 3368 3461 3368
Size: 93 5764
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2082 2170 2473 3044 3123 3176 3918
Size: 399 292 88 303 571 79 53 742
Cuts at: 3918 4003 5074 1391
Size: 85 1071 2174
Fragments arranged by size:
2174 1071 742 571 399 303 292 88
85 79 53
BspEI T'CCGG_A
Cuts at: 5520 5520
Size: 5857
BspHI T'CATG_A
Cuts at: 3746 5476 3746
Size: 1730 4127
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2619 3212 844
Size: 1775 593 3489
Fragments arranged by size:
3489 1775 593
BsrBI CCG'CTC
Cuts at: 1394 1823 3744 1394
Size: 429 1921 3507
Fragments arranged by size:
3507 1921 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4352 4526 65
Size: 1183 3104 174 1396
Fragments arranged by size:
3104 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2088 2357 3091 4501 4688 1700
Size: 110 278 269 734 1410 187 2869
Fragments arranged by size:
2869 1410 734 278 269 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5857
BssHII G'CGCG_C
Cuts at: 1925 2066 2189 2216 2218 2220 1925
Size: 141 123 27 2 2 5562
Fragments arranged by size:
5562 141 123 27 2 2
BssSI C'ACGA_G
Cuts at: 3917 4929 3917
Size: 1012 4845
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2299 794
Size: 1505 4352
BstBI TT'CG_AA
Cuts at: 4661 4661
Size: 5857
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5857
BstXI CCAn_nnnn'nTGG
Cuts at: 2827 2827
Size: 5857
BstYI r'GATC_y
Cuts at: 1972 2685 2802 2952 3187 3554 3939 3956 5408
Size: 713 117 150 235 367 385 17 1452
Cuts at: 5408 5494 5506 5852 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1452 713 385 367 346 235 150
117 86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2259 2363 513
Size: 552 1194 104 4007
Fragments arranged by size:
4007 1194 552 104
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5118
Fragments arranged by size:
5118 683 56
BtsI GCAGTG_nn'
Cuts at: 3373 4167 4187 3373
Size: 794 20 5043
Fragments arranged by size:
5043 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5857
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5822
DraI TTT'AAA
Cuts at: 3161 3161
Size: 5857
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4864 809
Size: 507 3548 1802
Fragments arranged by size:
3548 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2256 3144 3304
Size: 54 1425 50 275 18 426 888 160
Cuts at: 3304 4193 5822 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 889 888 426 275 160 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2256 3304 1537
Size: 275 444 1048 4090
Fragments arranged by size:
4090 1048 444 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3016 3787 1353
Size: 1663 771 3423
Fragments arranged by size:
3423 1663 771
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2846 4444 4818 4964 387
Size: 1064 138 1257 1598 374 146 1280
Fragments arranged by size:
1598 1280 1257 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 3121 3121
Size: 5857
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2882 3920 4517 5304 5572 1239
Size: 393 1250 1038 597 787 268 1524
Fragments arranged by size:
1524 1250 1038 787 597 393 268
EcoNI CCTnn'n_nnAGG
Cuts at: 2234 2864 2234
Size: 630 5227
EcoO109I rG'GnC_Cy
Cuts at: 2779 2779
Size: 5857
EcoRI G'AATT_C
Cuts at: 3166 3544 3166
Size: 378 5479
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2887 2887
Size: 5857
FspI TGC'GCA
Cuts at: 2039 4363 2039
Size: 2324 3533
HaeII r_GCGC'y
Cuts at: 1678 2032 2316 5004 1678
Size: 354 284 2688 2531
Fragments arranged by size:
2688 2531 354 284
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2460 2492 2705 2737 2763
Size: 32 135 32 888 32 213 32 26
Cuts at: 2763 2795 4498 4530 4572 4604 1373
Size: 32 1703 32 42 32 2626
Fragments arranged by size:
2626 1703 888 213 135 42 32 32
32 32 32 32 32 26
HincII GTy'rAC
Cuts at: 669 1657 1938 2140 3201 3447 669
Size: 988 281 202 1061 246 3079
Fragments arranged by size:
3079 1061 988 281 246 202
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5857
HpaI GTT'AAC
Cuts at: 3447 3447
Size: 5857
KasI G'GCGC_C
Cuts at: 2028 2028
Size: 5857
KpnI G_GTAC'C
Cuts at: 3182 3182
Size: 5857
MfeI C'AATT_G
Cuts at: 3456 3456
Size: 5857
MluI A'CGCG_T
Cuts at: 5733 5733
Size: 5857
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4971 5155 1305
Size: 3666 184 2007
Fragments arranged by size:
3666 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4035
Fragments arranged by size:
4035 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2590 2825 3815 4174 4333 518
Size: 648 1424 235 990 359 159 2042
Fragments arranged by size:
2042 1424 990 648 359 235 159
MspA1I CmG'CkG
Cuts at: 1606 2035 2365 3273 3950 5098 5343 1606
Size: 429 330 908 677 1148 245 2120
Fragments arranged by size:
2120 1148 908 677 429 330 245
NaeI GCC'GGC
Cuts at: 2090 2359 2090
Size: 269 5588
NarI GG'CG_CC
Cuts at: 2029 2029
Size: 5857
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5305
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5857
NgoMIV G'CCGG_C
Cuts at: 2088 2357 2088
Size: 269 5588
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5857
NotI GC'GGCC_GC
Cuts at: 3304 3304
Size: 5857
NsiI A_TGCA'T
Cuts at: 2587 2587
Size: 5857
NspI r_CATG'y
Cuts at: 1132 1229 3052 3215 4760 1132
Size: 97 1823 163 1545 2229
Fragments arranged by size:
2229 1823 1545 163 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5857
PciI A'CATG_T
Cuts at: 4756 4756
Size: 5857
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5857
PmeI GTTT'AAAC
Cuts at: 3161 3161
Size: 5857
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3926 3958 5439 5471 3926
Size: 32 1481 32 4312
Fragments arranged by size:
4312 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2779 2779
Size: 5857
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5857
PsiI TTA'TAA
Cuts at: 3427 3427
Size: 5857
PspOMI G'GGCC_C
Cuts at: 2166 2166
Size: 5857
PstI C_TGCA'G
Cuts at: 830 2235 2940 3209 830
Size: 1405 705 269 3478
Fragments arranged by size:
3478 1405 705 269
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5857
PvuII CAG'CTG
Cuts at: 2035 2035
Size: 5857
SacI G_AGCT'C
Cuts at: 3123 3123
Size: 5857
SacII CC_GC'GG
Cuts at: 2366 2366
Size: 5857
SalI G'TCGA_C
Cuts at: 1936 3199 1936
Size: 1263 4594
SapI GCTCTTCn'nnn_
Cuts at: 3016 3016
Size: 5857
SbfI CC_TGCA'GG
Cuts at: 2235 3209 2235
Size: 974 4883
ScaI AGT'ACT
Cuts at: 4105 5817 4105
Size: 1712 4145
SfcI C'TryA_G
Cuts at: 826 1045 2231 2512 2936 3205 4340 5021 5212
Size: 219 1186 281 424 269 1135 681 191
Cuts at: 5212 826
Size: 1471
Fragments arranged by size:
1471 1186 1135 681 424 281 269 219
191
SfoI GGC'GCC
Cuts at: 2030 2030
Size: 5857
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5857
SgrAI Cr'CCGG_yG
Cuts at: 1700 3091 1700
Size: 1391 4466
SmaI CCC'GGG
Cuts at: 1872 2171 3184 1872
Size: 299 1013 4545
Fragments arranged by size:
4545 1013 299
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3961 4862 5124 820
Size: 197 926 2018 901 262 1553
Fragments arranged by size:
2018 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3648 493
Size: 3155 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5857
SphI G_CATG'C
Cuts at: 1132 3215 1132
Size: 2083 3774
SspI AAT'ATT
Cuts at: 5 52 3781 5
Size: 47 3729 2081
Fragments arranged by size:
3729 2081 47
StuI AGG'CCT
Cuts at: 1517 2657 2870 1517
Size: 1140 213 4504
Fragments arranged by size:
4504 1140 213
StyI C'CwwG_G
Cuts at: 513 1065 2566 2992 3296 513
Size: 552 1501 426 304 3074
Fragments arranged by size:
3074 1501 552 426 304
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2830 3884 3901 4054 4239 4684 325
Size: 1255 1250 1054 17 153 185 445 1498
Fragments arranged by size:
1498 1255 1250 1054 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4103 5815 96
Size: 274 80 33 51 1443 2126 1712 138
Fragments arranged by size:
2126 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2481 2513 5817 5849 2481
Size: 32 3304 32 2489
Fragments arranged by size:
3304 2489 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 2756 1316
Size: 1440 4417
XbaI T'CTAG_A
Cuts at: 3193 3193
Size: 5857
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2795 2824 2795
Size: 29 5828
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5857
XmaI C'CCGG_G
Cuts at: 1870 2169 3182 1870
Size: 299 1013 4545
Fragments arranged by size:
4545 1013 299
XmnI GAAnn'nnTTC
Cuts at: 2550 3986 2550
Size: 1436 4421
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3665 276
Size: 53 83 186 936 2131 2468
Fragments arranged by size:
2468 2131 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI
BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI
BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI
FalI FspI HaeII Hin4I HincII HindIII HpaI KasI
KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI
NarI NcoI NdeI NgoMIV NheI NotI NsiI NspI
PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI
PspOMI PstI PvuI PvuII SacI SacII SalI SapI
SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AscI AvrII BaeI BbvCI BplI Bpu10I
BsiWI BsmBI BstZ17I Bsu36I DraIII EcoRV FseI FspAI
NruI PacI PfoI PmlI PspXI PsrI RsrII SanDI
SexAI SfiI SrfI SwaI