Restriction map for
pFN21AB5401
(Circular) (Six-base) MAPSORT of: pFN21AB5401.seq Check: 3595 from: 1 to: 5494
pFN21AB5401 5494 bp
With 182 enzymes: SgfI *
February 27, 2009 14:45 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2380 2380
Size: 5494
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3304 278
Size: 53 83 186 936 1768 2468
Fragments arranged by size:
2468 1768 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2837 1937
Size: 900 4594
Acc65I G'GTAC_C
Cuts at: 2815 2815
Size: 5494
AclI AA'CG_TT
Cuts at: 1563 3621 3994 1563
Size: 2058 373 3063
Fragments arranged by size:
3063 2058 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2385 3557 4941 5209 1632
Size: 753 1172 1384 268 1917
Fragments arranged by size:
1917 1384 1172 753 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5297
AflIII A'CryG_T
Cuts at: 1203 4393 5370 1203
Size: 3190 977 1327
Fragments arranged by size:
3190 1327 977
AgeI A'CCGG_T
Cuts at: 4325 4325
Size: 5494
AhdI GACnn_n'nnGTC
Cuts at: 4223 4223
Size: 5494
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5494
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5494
AlwNI CAG_nnn'CTG
Cuts at: 2211 2329 3237 4809 2211
Size: 118 908 1572 2896
Fragments arranged by size:
2896 1572 908 118
ApaI G_GGCC'C
Cuts at: 2244 2244
Size: 5494
ApaLI G'TGCA_C
Cuts at: 3551 4707 3551
Size: 1156 4338
ApoI r'AATT_y
Cuts at: 1493 2803 3029 3181 5290 1493
Size: 1310 226 152 2109 1697
Fragments arranged by size:
2109 1697 1310 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4048 160
Size: 872 3016 1606
Fragments arranged by size:
3016 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5494
AvaI C'yCGr_G
Cuts at: 1870 1943 2819 1870
Size: 73 876 4545
Fragments arranged by size:
4545 876 73
BamHI G'GATC_C
Cuts at: 2824 3191 2824
Size: 367 5127
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2815 4270 618
Size: 325 217 627 1028 1455 1842
Fragments arranged by size:
1842 1455 1028 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2244 2813 1391
Size: 853 569 4072
Fragments arranged by size:
4072 853 569
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2711 928
Size: 971 812 3711
Fragments arranged by size:
3711 971 812
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 3685 3719 1903
Size: 34 50 34 1664 34 3678
Fragments arranged by size:
3678 1664 50 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3386 4596 3386
Size: 1210 4284
BclI T'GATC_A
Cuts at: 1548 2541 2617 1548
Size: 993 76 4425
Fragments arranged by size:
4425 993 76
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4105 136
Size: 107 122 71 631 605 2433 1525
Fragments arranged by size:
2433 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5489 5489
Size: 5494
BlpI GC'TnA_GC
Cuts at: 2911 2911
Size: 5494
Bme1580I G_kGCm'C
Cuts at: 1790 2244 3555 4711 1790
Size: 454 1311 1156 2573
Fragments arranged by size:
2573 1311 1156 454
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5494
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3225 4183 442
Size: 416 53 83 399 24 1808 958 1753
Fragments arranged by size:
1808 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5494
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2335 2335
Size: 5494
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2091 2718 4154 1239
Size: 852 627 1436 2579
Fragments arranged by size:
2579 1436 852 627
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3619 4484 4782 3619
Size: 865 298 4331
Fragments arranged by size:
4331 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2306 4157 882
Size: 617 807 1851 2219
Fragments arranged by size:
2219 1851 807 617
BsaAI yAC'GTr
Cuts at: 493 1204 2518 3285 493
Size: 711 1314 767 2702
Fragments arranged by size:
2702 1314 767 711
BsaBI GATnn'nnATC
Cuts at: 2456 2540 5488 2456
Size: 84 2948 2462
Fragments arranged by size:
2948 2462 84
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2271 3301 3683 4318
Size: 53 83 186 936 738 1030 382 635
Cuts at: 4318 275
Size: 1451
Fragments arranged by size:
1451 1030 936 738 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 3927 4325 4599 4746 5157 3927
Size: 398 274 147 411 4264
Fragments arranged by size:
4264 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2102 2132 1178
Size: 30 165 30 699 30 4540
Fragments arranged by size:
4540 699 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 5494
BseYI C'CCAG_C
Cuts at: 2521 4697 5193 2521
Size: 2176 496 2822
Fragments arranged by size:
2822 2176 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2397 2408 2462 1163
Size: 573 661 11 54 4195
Fragments arranged by size:
4195 661 573 54 11
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2944 3705 4733 1540
Size: 275 186 943 761 1028 2301
Fragments arranged by size:
2301 1028 943 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3555 3640 4711 3555
Size: 85 1071 4338
Fragments arranged by size:
4338 1071 85
BsmI GAATG_Cn'
Cuts at: 2027 3005 3098 2027
Size: 978 93 4423
Fragments arranged by size:
4423 978 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2244 2813 3555 3640 4711 1391
Size: 399 454 569 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 569 454 399 85
BspEI T'CCGG_A
Cuts at: 5157 5157
Size: 5494
BspHI T'CATG_A
Cuts at: 3383 5113 3383
Size: 1730 3764
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2307 2380 2849 844
Size: 1463 73 469 3489
Fragments arranged by size:
3489 1463 469 73
BsrBI CCG'CTC
Cuts at: 1394 1823 2166 2420 3381 1394
Size: 429 343 254 961 3507
Fragments arranged by size:
3507 961 429 343 254
BsrDI GCAATG_nn'
Cuts at: 65 1248 2547 3989 4163 65
Size: 1183 1299 1442 174 1396
Fragments arranged by size:
1442 1396 1299 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2195 2267 4138 4325 1700
Size: 110 385 72 1871 187 2869
Fragments arranged by size:
2869 1871 385 187 110 72
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5494
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5494
BssSI C'ACGA_G
Cuts at: 3554 4566 3554
Size: 1012 4482
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5494
BstBI TT'CG_AA
Cuts at: 4298 4298
Size: 5494
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5494
BstXI CCAn_nnnn'nTGG
Cuts at: 2011 2011
Size: 5494
BstYI r'GATC_y
Cuts at: 1972 2066 2824 3191 3576 3593 5045 5131 5143
Size: 94 758 367 385 17 1452 86 12
Cuts at: 5143 5489 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 758 385 367 346 94 86
17 12
Bsu36I CC'TnA_GG
Cuts at: 2745 2745
Size: 5494
BtgI C'CryG_G
Cuts at: 513 1065 513
Size: 552 4942
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4755
Fragments arranged by size:
4755 683 56
BtsI GCAGTG_nn'
Cuts at: 3010 3804 3824 3010
Size: 794 20 4680
Fragments arranged by size:
4680 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5494
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5459
DraI TTT'AAA
Cuts at: 2798 2798
Size: 5494
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4501 809
Size: 507 3185 1802
Fragments arranged by size:
3185 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2092 2941 3830
Size: 54 1425 50 275 18 262 849 889
Cuts at: 3830 5459 8
Size: 1629 43
Fragments arranged by size:
1629 1425 889 849 275 262 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2941 1537
Size: 275 1129 4090
Fragments arranged by size:
4090 1129 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3424 1353
Size: 2071 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4081 4455 4601 387
Size: 1064 138 2492 374 146 1280
Fragments arranged by size:
2492 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2091 2385 2718 3557 4154 4941 5209
Size: 393 459 294 333 839 597 787 268
Cuts at: 5209 1239
Size: 1524
Fragments arranged by size:
1524 839 787 597 459 393 333 294
268
EcoNI CCTnn'n_nnAGG
Cuts at: 2587 2587
Size: 5494
EcoO109I rG'GnC_Cy
Cuts at: 2230 2241 2296 2326 2497 2230
Size: 11 55 30 171 5227
Fragments arranged by size:
5227 171 55 30 11
EcoRI G'AATT_C
Cuts at: 2803 3181 2803
Size: 378 5116
FspI TGC'GCA
Cuts at: 4000 4000
Size: 5494
HaeII r_GCGC'y
Cuts at: 1678 4641 1678
Size: 2963 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2102 2134 2550 2582 4135
Size: 32 135 32 530 32 416 32 1553
Cuts at: 4135 4167 4209 4241 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1553 530 416 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2740 2838 3084 669
Size: 988 281 802 98 246 3079
Fragments arranged by size:
3079 988 802 281 246 98
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5494
HpaI GTT'AAC
Cuts at: 3084 3084
Size: 5494
KpnI G_GTAC'C
Cuts at: 2819 2819
Size: 5494
MfeI C'AATT_G
Cuts at: 3093 3093
Size: 5494
MluI A'CGCG_T
Cuts at: 5370 5370
Size: 5494
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4608 4792 1305
Size: 3303 184 2007
Fragments arranged by size:
3303 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3672
Fragments arranged by size:
3672 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2531 2537 3452 3811 3970 518
Size: 648 1365 6 915 359 159 2042
Fragments arranged by size:
2042 1365 915 648 359 159 6
MspA1I CmG'CkG
Cuts at: 1606 2139 2438 2467 2910 3587 4735 4980 1606
Size: 533 299 29 443 677 1148 245 2120
Fragments arranged by size:
2120 1148 677 533 443 299 245 29
NaeI GCC'GGC
Cuts at: 2197 2269 2197
Size: 72 5422
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 4942
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5494
NgoMIV G'CCGG_C
Cuts at: 2195 2267 2195
Size: 72 5422
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5494
NotI GC'GGCC_GC
Cuts at: 2941 2941
Size: 5494
NspI r_CATG'y
Cuts at: 1132 1229 2047 2852 4397 1132
Size: 97 818 805 1545 2229
Fragments arranged by size:
2229 1545 818 805 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5494
PciI A'CATG_T
Cuts at: 4393 4393
Size: 5494
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2453 1108
Size: 1345 4149
PfoI T'CCnGG_A
Cuts at: 2352 2352
Size: 5494
PmeI GTTT'AAAC
Cuts at: 2798 2798
Size: 5494
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3563 3595 5076 5108 3563
Size: 32 1481 32 3949
Fragments arranged by size:
3949 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2230 2296 2497 2230
Size: 66 201 5227
Fragments arranged by size:
5227 201 66
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5494
PsiI TTA'TAA
Cuts at: 3064 3064
Size: 5494
PspOMI G'GGCC_C
Cuts at: 2240 2240
Size: 5494
PstI C_TGCA'G
Cuts at: 830 2846 830
Size: 2016 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5494
PvuII CAG'CTG
Cuts at: 2438 2438
Size: 5494
SalI G'TCGA_C
Cuts at: 1936 2836 1936
Size: 900 4594
SbfI CC_TGCA'GG
Cuts at: 2846 2846
Size: 5494
ScaI AGT'ACT
Cuts at: 3742 5454 3742
Size: 1712 3782
SfcI C'TryA_G
Cuts at: 826 1045 2842 3977 4658 4849 826
Size: 219 1797 1135 681 191 1471
Fragments arranged by size:
1797 1471 1135 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5494
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5494
SmaI CCC'GGG
Cuts at: 1872 2821 1872
Size: 949 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3598 4499 4761 820
Size: 197 926 1655 901 262 1553
Fragments arranged by size:
1655 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3285 493
Size: 2792 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5494
SphI G_CATG'C
Cuts at: 1132 2852 1132
Size: 1720 3774
SspI AAT'ATT
Cuts at: 5 52 3418 5
Size: 47 3366 2081
Fragments arranged by size:
3366 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5494
StyI C'CwwG_G
Cuts at: 513 1065 2484 2933 513
Size: 552 1419 449 3074
Fragments arranged by size:
3074 1419 552 449
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3521 3538 3691 3876 4321 325
Size: 1255 1941 17 153 185 445 1498
Fragments arranged by size:
1941 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3740 5452 96
Size: 274 80 33 51 1443 1763 1712 138
Fragments arranged by size:
1763 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5454 5486 5454
Size: 32 5462
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5494
XbaI T'CTAG_A
Cuts at: 2830 2830
Size: 5494
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5494
XmaI C'CCGG_G
Cuts at: 1870 2819 1870
Size: 949 4545
XmnI GAAnn'nnTTC
Cuts at: 3623 3623
Size: 5494
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3302 276
Size: 53 83 186 936 1768 2468
Fragments arranged by size:
2468 1768 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I
BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI
EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FspI
HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI
MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV
NheI NotI NspI PasI PciI PflMI PfoI PmeI
PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII
SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI AscI AvrII BaeI BbeI BbvCI BfrBI
Bpu10I BsiWI BsmBI BstZ17I DraIII EcoICRI EcoRV FalI
FseI FspAI KasI NarI NruI NsiI PacI PmlI
PspXI PsrI RsrII SacI SacII SanDI SapI SexAI
SfiI SfoI SrfI SwaI XcmI