Restriction map for
pFN21AB5400
(Circular) (Six-base) MAPSORT of: pFN21AB5400.seq Check: 1734 from: 1 to: 6118
pFN21AB5400 6118 bp
With 182 enzymes: SgfI *
October 20, 2009 10:35 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3928 278
Size: 53 83 186 936 2392 2468
Fragments arranged by size:
2468 2392 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3461 1937
Size: 1524 4594
Acc65I G'GTAC_C
Cuts at: 3230 3439 3230
Size: 209 5909
AclI AA'CG_TT
Cuts at: 1563 4245 4618 1563
Size: 2682 373 3063
Fragments arranged by size:
3063 2682 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2201 4181 5565 5833 1632
Size: 569 1980 1384 268 1917
Fragments arranged by size:
1980 1917 1384 569 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5921
AflIII A'CryG_T
Cuts at: 1203 5017 5994 1203
Size: 3814 977 1327
Fragments arranged by size:
3814 1327 977
AgeI A'CCGG_T
Cuts at: 4949 4949
Size: 6118
AhdI GACnn_n'nnGTC
Cuts at: 4847 4847
Size: 6118
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6118
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6118
AlwNI CAG_nnn'CTG
Cuts at: 3251 3380 3861 5433 3251
Size: 129 481 1572 3936
Fragments arranged by size:
3936 1572 481 129
ApaLI G'TGCA_C
Cuts at: 4175 5331 4175
Size: 1156 4962
ApoI r'AATT_y
Cuts at: 1493 2185 3389 3427 3653 3805 5914 1493
Size: 692 1204 38 226 152 2109 1697
Fragments arranged by size:
2109 1697 1204 692 226 152 38
AseI AT'TA_AT
Cuts at: 160 1032 4672 160
Size: 872 3640 1606
Fragments arranged by size:
3640 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6118
AvaI C'yCGr_G
Cuts at: 1870 1943 3443 1870
Size: 73 1500 4545
Fragments arranged by size:
4545 1500 73
BamHI G'GATC_C
Cuts at: 3448 3815 3448
Size: 367 5751
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2958 3051 3200 3230 3439
Size: 325 217 627 1171 93 149 30 209
Cuts at: 3439 4894 618
Size: 1455 1842
Fragments arranged by size:
1842 1455 1171 627 325 217 209 149
93 30
BanII G_rGCy'C
Cuts at: 1391 2682 3030 3437 1391
Size: 1291 348 407 4072
Fragments arranged by size:
4072 1291 407 348
BbeI G_GCGC'C
Cuts at: 2962 3055 2962
Size: 93 6025
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2213 2305 928
Size: 971 314 92 4741
Fragments arranged by size:
4741 971 314 92
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 3131 3165 4309 4343 1903
Size: 34 50 34 1110 34 1144 34 3678
Fragments arranged by size:
3678 1144 1110 50 34 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4010 5220 4010
Size: 1210 4908
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6118
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4729 136
Size: 107 122 71 631 605 3057 1525
Fragments arranged by size:
3057 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6113 6113
Size: 6118
BlpI GC'TnA_GC
Cuts at: 3535 3535
Size: 6118
Bme1580I G_kGCm'C
Cuts at: 1790 4179 5335 1790
Size: 2389 1156 2573
Fragments arranged by size:
2573 2389 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6118
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3849 4807 442
Size: 416 53 83 399 24 2432 958 1753
Fragments arranged by size:
2432 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6118
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2912 3044 3228 3402 4778 1239
Size: 1673 132 184 174 1376 2579
Fragments arranged by size:
2579 1673 1376 184 174 132
Bpu10I CC'TnA_GC
Cuts at: 3029 3029
Size: 6118
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4243 5108 5406 4243
Size: 865 298 4955
Fragments arranged by size:
4955 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4781 882
Size: 617 3282 2219
Fragments arranged by size:
3282 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3909 493
Size: 711 2705 2702
Fragments arranged by size:
2705 2702 711
BsaBI GATnn'nnATC
Cuts at: 6112 6112
Size: 6118
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2959 3052 3925 4307
Size: 53 83 186 936 1426 93 873 382
Cuts at: 4307 4942 275
Size: 635 1451
Fragments arranged by size:
1451 1426 936 873 635 382 186 93
83 53
BsaWI w'CCGG_w
Cuts at: 4551 4949 5223 5370 5781 4551
Size: 398 274 147 411 4888
Fragments arranged by size:
4888 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 3223 3253 1178
Size: 30 165 30 1820 30 4043
Fragments arranged by size:
4043 1820 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 3231 3345 1186
Size: 2045 114 3959
Fragments arranged by size:
3959 2045 114
BseYI C'CCAG_C
Cuts at: 3235 5321 5817 3235
Size: 2086 496 3536
Fragments arranged by size:
3536 2086 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2895 2975 3393 1163
Size: 573 1159 80 418 3888
Fragments arranged by size:
3888 1159 573 418 80
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3568 4329 5357 1540
Size: 275 186 1567 761 1028 2301
Fragments arranged by size:
2301 1567 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2925 3030 4179 4264 5335 2925
Size: 105 1149 85 1071 3708
Fragments arranged by size:
3708 1149 1071 105 85
BsmI GAATG_Cn'
Cuts at: 3362 3629 3722 3362
Size: 267 93 5758
Fragments arranged by size:
5758 267 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2682 2925 3030 3437 4179 4264 5335
Size: 399 892 243 105 407 742 85 1071
Cuts at: 5335 1391
Size: 2174
Fragments arranged by size:
2174 1071 892 742 407 399 243 105
85
BspEI T'CCGG_A
Cuts at: 5781 5781
Size: 6118
BspHI T'CATG_A
Cuts at: 4007 5737 4007
Size: 1730 4388
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2137 3259 3473 844
Size: 1293 1122 214 3489
Fragments arranged by size:
3489 1293 1122 214
BsrBI CCG'CTC
Cuts at: 1394 1823 4005 1394
Size: 429 2182 3507
Fragments arranged by size:
3507 2182 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4613 4787 65
Size: 1183 3365 174 1396
Fragments arranged by size:
3365 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4762 4949 1700
Size: 110 2952 187 2869
Fragments arranged by size:
2952 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6118
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6118
BssSI C'ACGA_G
Cuts at: 2880 4178 5190 2880
Size: 1298 1012 3808
Fragments arranged by size:
3808 1298 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6118
BstBI TT'CG_AA
Cuts at: 2461 4922 2461
Size: 2461 3657
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6118
BstYI r'GATC_y
Cuts at: 1972 3448 3815 4200 4217 5669 5755 5767 6113
Size: 1476 367 385 17 1452 86 12 346
Cuts at: 6113 1972
Size: 1977
Fragments arranged by size:
1977 1476 1452 385 367 346 86 17
12
Bsu36I CC'TnA_GG
Cuts at: 2566 2566
Size: 6118
BtgI C'CryG_G
Cuts at: 513 1065 2407 2682 513
Size: 552 1342 275 3949
Fragments arranged by size:
3949 1342 552 275
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5379
Fragments arranged by size:
5379 683 56
BtsI GCAGTG_nn'
Cuts at: 2635 3634 4428 4448 2635
Size: 999 794 20 4305
Fragments arranged by size:
4305 999 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6118
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6083
DraI TTT'AAA
Cuts at: 3422 3422
Size: 6118
DraIII CAC_nnn'GTG
Cuts at: 2408 2408
Size: 6118
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5125 809
Size: 507 3809 1802
Fragments arranged by size:
3809 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2518 3565 4454
Size: 54 1425 50 275 18 688 1047 889
Cuts at: 4454 6083 8
Size: 1629 43
Fragments arranged by size:
1629 1425 1047 889 688 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3565 1537
Size: 275 1753 4090
Fragments arranged by size:
4090 1753 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2697 2979 3036 3057 4048 1353
Size: 1344 282 57 21 991 3423
Fragments arranged by size:
3423 1344 991 282 57 21
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4705 5079 5225 387
Size: 1064 138 3116 374 146 1280
Fragments arranged by size:
3116 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 3028 3028
Size: 6118
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2201 2912 3044 3228 3402 4181 4778
Size: 393 569 711 132 184 174 779 597
Cuts at: 4778 5565 5833 1239
Size: 787 268 1524
Fragments arranged by size:
1524 787 779 711 597 569 393 268
184 174 132
EcoO109I rG'GnC_Cy
Cuts at: 2562 3248 2562
Size: 686 5432
EcoRI G'AATT_C
Cuts at: 3427 3805 3427
Size: 378 5740
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2726 2987 3139 2726
Size: 261 152 5705
Fragments arranged by size:
5705 261 152
FspI TGC'GCA
Cuts at: 4624 4624
Size: 6118
HaeII r_GCGC'y
Cuts at: 1678 2962 3055 5265 1678
Size: 1284 93 2210 2531
Fragments arranged by size:
2531 2210 1284 93
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 4759 4791 4833 4865 1373
Size: 32 135 32 3187 32 42 32 2626
Fragments arranged by size:
3187 2626 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3462 3708 669
Size: 988 281 1524 246 3079
Fragments arranged by size:
3079 1524 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6118
HpaI GTT'AAC
Cuts at: 3708 3708
Size: 6118
KasI G'GCGC_C
Cuts at: 2958 3051 2958
Size: 93 6025
KpnI G_GTAC'C
Cuts at: 3234 3443 3234
Size: 209 5909
MfeI C'AATT_G
Cuts at: 3342 3717 3342
Size: 375 5743
MluI A'CGCG_T
Cuts at: 5994 5994
Size: 6118
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2508 5232 5416 1305
Size: 1203 2724 184 2007
Fragments arranged by size:
2724 2007 1203 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2520 10
Size: 54 1425 343 688 3608
Fragments arranged by size:
3608 1425 688 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2831 2936 3260 4076 4435 4594 518
Size: 648 1665 105 324 816 359 159 2042
Fragments arranged by size:
2042 1665 816 648 359 324 159 105
MspA1I CmG'CkG
Cuts at: 1606 2419 2603 3227 3534 4211 5359 5604 1606
Size: 813 184 624 307 677 1148 245 2120
Fragments arranged by size:
2120 1148 813 677 624 307 245 184
NarI GG'CG_CC
Cuts at: 2959 3052 2959
Size: 93 6025
NcoI C'CATG_G
Cuts at: 513 1065 2682 513
Size: 552 1617 3949
Fragments arranged by size:
3949 1617 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6118
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6118
NotI GC'GGCC_GC
Cuts at: 3565 3565
Size: 6118
NspI r_CATG'y
Cuts at: 1132 1229 3476 5021 1132
Size: 97 2247 1545 2229
Fragments arranged by size:
2247 2229 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6118
PciI A'CATG_T
Cuts at: 5017 5017
Size: 6118
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2408 2814 1108
Size: 1300 406 4412
Fragments arranged by size:
4412 1300 406
PfoI T'CCnGG_A
Cuts at: 3244 3379 3244
Size: 135 5983
PmeI GTTT'AAAC
Cuts at: 3422 3422
Size: 6118
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4187 4219 5700 5732 4187
Size: 32 1481 32 4573
Fragments arranged by size:
4573 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 3248 3248
Size: 6118
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6118
PsiI TTA'TAA
Cuts at: 2729 3688 2729
Size: 959 5159
PstI C_TGCA'G
Cuts at: 830 3470 830
Size: 2640 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6118
PvuII CAG'CTG
Cuts at: 2419 2603 3227 2419
Size: 184 624 5310
Fragments arranged by size:
5310 624 184
SacI G_AGCT'C
Cuts at: 3030 3030
Size: 6118
SalI G'TCGA_C
Cuts at: 1936 3460 1936
Size: 1524 4594
SapI GCTCTTCn'nnn_
Cuts at: 2979 3036 2979
Size: 57 6061
SbfI CC_TGCA'GG
Cuts at: 3470 3470
Size: 6118
ScaI AGT'ACT
Cuts at: 4366 6078 4366
Size: 1712 4406
SexAI A'CCwGG_T
Cuts at: 2925 2925
Size: 6118
SfcI C'TryA_G
Cuts at: 826 1045 2535 3466 4601 5282 5473 826
Size: 219 1490 931 1135 681 191 1471
Fragments arranged by size:
1490 1471 1135 931 681 219 191
SfoI GGC'GCC
Cuts at: 2960 3053 2960
Size: 93 6025
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6118
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6118
SmaI CCC'GGG
Cuts at: 1872 3445 1872
Size: 1573 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4222 5123 5385 820
Size: 197 926 2279 901 262 1553
Fragments arranged by size:
2279 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3909 493
Size: 3416 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6118
SphI G_CATG'C
Cuts at: 1132 3476 1132
Size: 2344 3774
SspI AAT'ATT
Cuts at: 5 52 4042 5
Size: 47 3990 2081
Fragments arranged by size:
3990 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6118
StyI C'CwwG_G
Cuts at: 513 1065 2682 3557 513
Size: 552 1617 875 3074
Fragments arranged by size:
3074 1617 875 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2231 2782 4145 4162 4315 4500 4945
Size: 1255 651 551 1363 17 153 185 445
Cuts at: 4945 325
Size: 1498
Fragments arranged by size:
1498 1363 1255 651 551 445 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4364 6076 96
Size: 274 80 33 51 1443 2387 1712 138
Fragments arranged by size:
2387 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6078 6110 6078
Size: 32 6086
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6118
XbaI T'CTAG_A
Cuts at: 3454 3454
Size: 6118
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2386 2491 2386
Size: 105 6013
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6118
XmaI C'CCGG_G
Cuts at: 1870 3443 1870
Size: 1573 4545
XmnI GAAnn'nnTTC
Cuts at: 2210 2970 4247 2210
Size: 760 1277 4081
Fragments arranged by size:
4081 1277 760
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3926 276
Size: 53 83 186 936 2392 2468
Fragments arranged by size:
2468 2392 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI
BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI
HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI
MluI MmeI MscI MslI MspA1I NarI NcoI NdeI
NheI NotI NspI PasI PciI PflMI PfoI PmeI
PpiI PpuMI PshAI PsiI PstI PvuI PvuII SacI
SalI SapI SbfI ScaI SexAI SfcI SfoI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbvCI
BfrBI BplI BsiWI BsmBI BstXI BstZ17I EcoNI EcoRV
FseI FspAI NaeI NgoMIV NruI NsiI PacI PmlI
PspOMI PspXI PsrI RsrII SacII SanDI SfiI SrfI
SwaI