Restriction map for
pFN21AB5396
(Circular) (Six-base) MAPSORT of: pFN21AB5396.seq Check: 7392 from: 1 to: 6604
pFN21AB5396 6604 bp
With 182 enzymes: SgfI *
February 27, 2009 14:34 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4414 278
Size: 53 83 186 936 2878 2468
Fragments arranged by size:
2878 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3947 1937
Size: 2010 4594
Acc65I G'GTAC_C
Cuts at: 3925 3925
Size: 6604
AclI AA'CG_TT
Cuts at: 1563 4731 5104 1563
Size: 3168 373 3063
Fragments arranged by size:
3168 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3800 4667 6051 6319 1632
Size: 2168 867 1384 268 1917
Fragments arranged by size:
2168 1917 1384 867 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6407
AflIII A'CryG_T
Cuts at: 1203 2272 5503 6480 1203
Size: 1069 3231 977 1327
Fragments arranged by size:
3231 1327 1069 977
AgeI A'CCGG_T
Cuts at: 5435 5435
Size: 6604
AhdI GACnn_n'nnGTC
Cuts at: 5333 5333
Size: 6604
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6604
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6604
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3773 3805 3773
Size: 32 6572
AlwNI CAG_nnn'CTG
Cuts at: 4347 5919 4347
Size: 1572 5032
ApaLI G'TGCA_C
Cuts at: 4661 5817 4661
Size: 1156 5448
ApoI r'AATT_y
Cuts at: 1493 2213 2694 2798 3092 3869 3913 4139 4291
Size: 720 481 104 294 777 44 226 152
Cuts at: 4291 6400 1493
Size: 2109 1697
Fragments arranged by size:
2109 1697 777 720 481 294 226 152
104 44
AseI AT'TA_AT
Cuts at: 160 1032 5158 160
Size: 872 4126 1606
Fragments arranged by size:
4126 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6604
AvaI C'yCGr_G
Cuts at: 1870 1943 3687 3929 1870
Size: 73 1744 242 4545
Fragments arranged by size:
4545 1744 242 73
BamHI G'GATC_C
Cuts at: 2599 2756 3934 4301 2599
Size: 157 1178 367 4902
Fragments arranged by size:
4902 1178 367 157
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2548 3604 3925 5380 618
Size: 325 217 627 761 1056 321 1455 1842
Fragments arranged by size:
1842 1455 1056 761 627 325 321 217
BanII G_rGCy'C
Cuts at: 1391 2233 3923 1391
Size: 842 1690 4072
Fragments arranged by size:
4072 1690 842
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2318 3156 928
Size: 971 419 838 4376
Fragments arranged by size:
4376 971 838 419
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4795 4829 1903
Size: 34 2858 34 3678
Fragments arranged by size:
3678 2858 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2056 3874 4496 5706 2056
Size: 1818 622 1210 2954
Fragments arranged by size:
2954 1818 1210 622
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6604
BfrBI ATG'CAT
Cuts at: 2630 3134 2630
Size: 504 6100
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 5215 136
Size: 107 122 71 631 605 3543 1525
Fragments arranged by size:
3543 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6599 6599
Size: 6604
BlpI GC'TnA_GC
Cuts at: 3804 4021 3804
Size: 217 6387
Bme1580I G_kGCm'C
Cuts at: 1790 2553 3607 4665 5821 1790
Size: 763 1054 1058 1156 2573
Fragments arranged by size:
2573 1156 1058 1054 763
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6604
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3793 4335 5293
Size: 416 53 83 399 24 2376 542 958
Cuts at: 5293 442
Size: 1753
Fragments arranged by size:
2376 1753 958 542 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6604
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 3265 3372 5264 1239
Size: 2026 107 1892 2579
Fragments arranged by size:
2579 2026 1892 107
Bpu10I CC'TnA_GC
Cuts at: 3018 3018
Size: 6604
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2417 4729 5594 5892 2417
Size: 2312 865 298 3129
Fragments arranged by size:
3129 2312 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 5267 882
Size: 617 3768 2219
Fragments arranged by size:
3768 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 4395 493
Size: 711 3191 2702
Fragments arranged by size:
3191 2702 711
BsaBI GATnn'nnATC
Cuts at: 6598 6598
Size: 6604
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2923 4411 4793 5428
Size: 53 83 186 936 1390 1488 382 635
Cuts at: 5428 275
Size: 1451
Fragments arranged by size:
1488 1451 1390 936 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 2609 5037 5435 5709 5856 6267 2609
Size: 2428 398 274 147 411 2946
Fragments arranged by size:
2946 2428 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 3150 3180 1178
Size: 30 165 30 1747 30 4602
Fragments arranged by size:
4602 1747 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 3171 3309 3312 3525 3528 1186
Size: 1985 138 3 213 3 4262
Fragments arranged by size:
4262 1985 213 138 3 3
BseYI C'CCAG_C
Cuts at: 5807 6303 5807
Size: 496 6108
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2643 1163
Size: 573 907 5124
Fragments arranged by size:
5124 907 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4054 4815 5843 1540
Size: 275 186 2053 761 1028 2301
Fragments arranged by size:
2301 2053 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2233 3538 3565 4665 4750 5821 2233
Size: 1305 27 1100 85 1071 3016
Fragments arranged by size:
3016 1305 1100 1071 85 27
BsmI GAATG_Cn'
Cuts at: 4115 4208 4115
Size: 93 6511
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2233 2553 3538 3565 3607 3923 4665
Size: 399 443 320 985 27 42 316 742
Cuts at: 4665 4750 5821 1391
Size: 85 1071 2174
Fragments arranged by size:
2174 1071 985 742 443 399 320 316
85 42 27
BspEI T'CCGG_A
Cuts at: 6267 6267
Size: 6604
BspHI T'CATG_A
Cuts at: 4493 6223 4493
Size: 1730 4874
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3959 844
Size: 3115 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 4491 1394
Size: 429 2668 3507
Fragments arranged by size:
3507 2668 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3712 5099 5273 65
Size: 1183 2464 1387 174 1396
Fragments arranged by size:
2464 1396 1387 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2925 5248 5435 1700
Size: 110 1115 2323 187 2869
Fragments arranged by size:
2869 2323 1115 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6604
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6604
BssSI C'ACGA_G
Cuts at: 4664 5676 4664
Size: 1012 5592
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2201 794
Size: 1407 5197
BstBI TT'CG_AA
Cuts at: 5408 5408
Size: 6604
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6604
BstYI r'GATC_y
Cuts at: 1972 2581 2599 2756 3001 3466 3683 3934 4301
Size: 609 18 157 245 465 217 251 367
Cuts at: 4301 4686 4703 6155 6241 6253 6599 1972
Size: 385 17 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 609 465 385 367 346 251
245 217 157 86 18 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 3670 3679 513
Size: 552 939 1666 9 3438
Fragments arranged by size:
3438 1666 939 552 9
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 3784 487
Size: 683 56 2558 3307
Fragments arranged by size:
3307 2558 683 56
BtsI GCAGTG_nn'
Cuts at: 3288 4120 4914 4934 3288
Size: 832 794 20 4958
Fragments arranged by size:
4958 832 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6604
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6569
DraI TTT'AAA
Cuts at: 3908 3908
Size: 6604
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5611 809
Size: 507 4295 1802
Fragments arranged by size:
4295 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 4051 4940 6569
Size: 54 1425 50 275 18 2221 889 1629
Cuts at: 6569 8
Size: 43
Fragments arranged by size:
2221 1629 1425 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 4051 1537
Size: 275 2239 4090
Fragments arranged by size:
4090 2239 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2040 3502 4534 1353
Size: 687 1462 1032 3423
Fragments arranged by size:
3423 1462 1032 687
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5191 5565 5711 387
Size: 1064 138 3602 374 146 1280
Fragments arranged by size:
3602 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2231 2231
Size: 6604
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 3265 3372 3800 4667 5264 6051 6319
Size: 393 1633 107 428 867 597 787 268
Cuts at: 6319 1239
Size: 1524
Fragments arranged by size:
1633 1524 867 787 597 428 393 268
107
EcoNI CCTnn'n_nnAGG
Cuts at: 3573 3573
Size: 6604
EcoO109I rG'GnC_Cy
Cuts at: 2366 3647 2366
Size: 1281 5323
EcoRI G'AATT_C
Cuts at: 2798 3913 4291 2798
Size: 1115 378 5111
Fragments arranged by size:
5111 1115 378
FspI TGC'GCA
Cuts at: 5110 5110
Size: 6604
HaeII r_GCGC'y
Cuts at: 1678 3262 5751 1678
Size: 1584 2489 2531
Fragments arranged by size:
2531 2489 1584
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2591 2623 2888 2920 5245
Size: 32 135 32 1019 32 265 32 2325
Cuts at: 5245 5277 5319 5351 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 2325 1019 265 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3948 4194 669
Size: 988 281 2010 246 3079
Fragments arranged by size:
3079 2010 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6604
HpaI GTT'AAC
Cuts at: 4194 4194
Size: 6604
KpnI G_GTAC'C
Cuts at: 3929 3929
Size: 6604
MfeI C'AATT_G
Cuts at: 2196 4203 2196
Size: 2007 4597
MluI A'CGCG_T
Cuts at: 6480 6480
Size: 6604
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2734 5718 5902 1305
Size: 1429 2984 184 2007
Fragments arranged by size:
2984 2007 1429 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4782
Fragments arranged by size:
4782 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2296 2729 4562 4921 5080 518
Size: 648 1130 433 1833 359 159 2042
Fragments arranged by size:
2042 1833 1130 648 433 359 159
MspA1I CmG'CkG
Cuts at: 1606 3164 4020 4697 5845 6090 1606
Size: 1558 856 677 1148 245 2120
Fragments arranged by size:
2120 1558 1148 856 677 245
NaeI GCC'GGC
Cuts at: 2927 2927
Size: 6604
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 5113
Fragments arranged by size:
5113 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6604
NgoMIV G'CCGG_C
Cuts at: 2925 2925
Size: 6604
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6604
NotI GC'GGCC_GC
Cuts at: 4051 4051
Size: 6604
NsiI A_TGCA'T
Cuts at: 2632 3136 2632
Size: 504 6100
NspI r_CATG'y
Cuts at: 1132 1229 2276 3331 3962 5507 1132
Size: 97 1047 1055 631 1545 2229
Fragments arranged by size:
2229 1545 1055 1047 631 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6604
PciI A'CATG_T
Cuts at: 2272 5503 2272
Size: 3231 3373
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6604
PmeI GTTT'AAAC
Cuts at: 3908 3908
Size: 6604
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4673 4705 6186 6218 4673
Size: 32 1481 32 5059
Fragments arranged by size:
5059 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2366 3647 2366
Size: 1281 5323
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6604
PsiI TTA'TAA
Cuts at: 4174 4174
Size: 6604
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 2395 2427 2395
Size: 32 6572
PstI C_TGCA'G
Cuts at: 830 3956 830
Size: 3126 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6604
PvuII CAG'CTG
Cuts at: 3164 3164
Size: 6604
SacI G_AGCT'C
Cuts at: 2233 2233
Size: 6604
SalI G'TCGA_C
Cuts at: 1936 3946 1936
Size: 2010 4594
SbfI CC_TGCA'GG
Cuts at: 3956 3956
Size: 6604
ScaI AGT'ACT
Cuts at: 4852 6564 4852
Size: 1712 4892
SfcI C'TryA_G
Cuts at: 826 1045 2243 2317 3952 5087 5768 5959 826
Size: 219 1198 74 1635 1135 681 191 1471
Fragments arranged by size:
1635 1471 1198 1135 681 219 191 74
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6604
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6604
SmaI CCC'GGG
Cuts at: 1872 3931 1872
Size: 2059 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2396 4708 5609 5871 820
Size: 197 926 453 2312 901 262 1553
Fragments arranged by size:
2312 1553 926 901 453 262 197
SnaBI TAC'GTA
Cuts at: 493 4395 493
Size: 3902 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6604
SphI G_CATG'C
Cuts at: 1132 3331 3962 1132
Size: 2199 631 3774
Fragments arranged by size:
3774 2199 631
SspI AAT'ATT
Cuts at: 5 52 2151 3072 4528 5
Size: 47 2099 921 1456 2081
Fragments arranged by size:
2099 2081 1456 921 47
StuI AGG'CCT
Cuts at: 1517 2115 2390 3278 1517
Size: 598 275 888 4843
Fragments arranged by size:
4843 888 598 275
StyI C'CwwG_G
Cuts at: 513 1065 2004 2522 2713 3548 4043 513
Size: 552 939 518 191 835 495 3074
Fragments arranged by size:
3074 939 835 552 518 495 191
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 4631 4648 4801 4986 5431 325
Size: 1255 3051 17 153 185 445 1498
Fragments arranged by size:
3051 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2239 2469 4850
Size: 274 80 33 51 1443 262 230 2381
Cuts at: 4850 6562 96
Size: 1712 138
Fragments arranged by size:
2381 1712 1443 274 262 230 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2431 2463 6564 6596 2431
Size: 32 4101 32 2439
Fragments arranged by size:
4101 2439 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6604
XbaI T'CTAG_A
Cuts at: 3940 3940
Size: 6604
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6604
XmaI C'CCGG_G
Cuts at: 1870 3929 1870
Size: 2059 4545
XmnI GAAnn'nnTTC
Cuts at: 2151 4733 2151
Size: 2582 4022
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4412 276
Size: 53 83 186 936 2878 2468
Fragments arranged by size:
2878 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI
BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI
BtsI ClaI CspCI DraI DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII
Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI
MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI
NotI NsiI NspI PasI PciI PflMI PmeI PpiI
PpuMI PshAI PsiI PsrI PstI PvuI PvuII SacI
SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI ApaI AscI AvrII BaeI BbeI BbvCI
BplI BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoRV
FalI FseI FspAI KasI NarI NruI PacI PfoI
PmlI PspOMI PspXI RsrII SacII SanDI SapI SexAI
SfiI SfoI SrfI SwaI XcmI