Restriction map for
pFN21AB5394
(Circular) (Six-base) MAPSORT of: pFN21AB5394.seq Check: 5134 from: 1 to: 6484
pFN21AB5394 6484 bp
With 182 enzymes: SgfI *
April 15, 2010 09:35 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4294 278
Size: 53 83 186 936 2758 2468
Fragments arranged by size:
2758 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3827 1937
Size: 1890 4594
Acc65I G'GTAC_C
Cuts at: 3805 3805
Size: 6484
AclI AA'CG_TT
Cuts at: 1563 4611 4984 1563
Size: 3048 373 3063
Fragments arranged by size:
3063 3048 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3690 4547 5931 6199 1632
Size: 2058 857 1384 268 1917
Fragments arranged by size:
2058 1917 1384 857 268
AfeI AGC'GCT
Cuts at: 3313 3313
Size: 6484
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6287
AflIII A'CryG_T
Cuts at: 1203 2385 2724 3096 5383 6360 1203
Size: 1182 339 372 2287 977 1327
Fragments arranged by size:
2287 1327 1182 977 372 339
AgeI A'CCGG_T
Cuts at: 5315 5315
Size: 6484
AhdI GACnn_n'nnGTC
Cuts at: 2413 5213 2413
Size: 2800 3684
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6484
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2201 1184
Size: 1017 5467
AlwNI CAG_nnn'CTG
Cuts at: 2101 2356 2371 3506 4227 5799 2101
Size: 255 15 1135 721 1572 2786
Fragments arranged by size:
2786 1572 1135 721 255 15
ApaLI G'TGCA_C
Cuts at: 4541 5697 4541
Size: 1156 5328
ApoI r'AATT_y
Cuts at: 1493 2152 3793 4019 4171 6280 1493
Size: 659 1641 226 152 2109 1697
Fragments arranged by size:
2109 1697 1641 659 226 152
AseI AT'TA_AT
Cuts at: 160 1032 5038 160
Size: 872 4006 1606
Fragments arranged by size:
4006 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6484
AvaI C'yCGr_G
Cuts at: 1870 1943 2927 2975 3145 3758 3809 1870
Size: 73 984 48 170 613 51 4545
Fragments arranged by size:
4545 984 613 170 73 51 48
BamHI G'GATC_C
Cuts at: 3814 4181 3814
Size: 367 6117
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2177 2466 2559 3223 3323
Size: 325 217 627 390 289 93 664 100
Cuts at: 3323 3515 3805 5260 618
Size: 192 290 1455 1842
Fragments arranged by size:
1842 1455 664 627 390 325 290 289
217 192 100 93
BanII G_rGCy'C
Cuts at: 1391 2280 2631 2894 3215 3270 3309 3427 3803
Size: 889 351 263 321 55 39 118 376
Cuts at: 3803 1391
Size: 4072
Fragments arranged by size:
4072 889 376 351 321 263 118 55
39
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3745 928
Size: 971 1846 3667
Fragments arranged by size:
3667 1846 971
BbvCI CC'TCA_GC
Cuts at: 3080 3080
Size: 6484
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3369 3403 4675 4709 1903
Size: 34 1432 34 1272 34 3678
Fragments arranged by size:
3678 1432 1272 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4376 5586 4376
Size: 1210 5274
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6484
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2284 2966 5095
Size: 107 122 71 631 605 612 682 2129
Cuts at: 5095 136
Size: 1525
Fragments arranged by size:
2129 1525 682 631 612 605 122 107
71
BglII A'GATC_T
Cuts at: 6479 6479
Size: 6484
BlpI GC'TnA_GC
Cuts at: 3901 3901
Size: 6484
Bme1580I G_kGCm'C
Cuts at: 1790 2180 2464 3326 4545 5701 1790
Size: 390 284 862 1219 1156 2573
Fragments arranged by size:
2573 1219 1156 862 390 284
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6484
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2548 2853 3127
Size: 416 53 83 399 24 1131 305 274
Cuts at: 3127 3776 4215 5173 442
Size: 649 439 958 1753
Fragments arranged by size:
1753 1131 958 649 439 416 399 305
274 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6484
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2078 2768 2834 2886 3441 3448 3755 5144
Size: 839 690 66 52 555 7 307 1389
Cuts at: 5144 1239
Size: 2579
Fragments arranged by size:
2579 1389 839 690 555 307 66 52
7
Bpu10I CC'TnA_GC
Cuts at: 3080 3080
Size: 6484
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2558 3611 4609 5474 5772 2558
Size: 1053 998 865 298 3270
Fragments arranged by size:
3270 1053 998 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2111 3485 5147 882
Size: 617 612 1374 1662 2219
Fragments arranged by size:
2219 1662 1374 617 612
BsaAI yAC'GTr
Cuts at: 493 1204 2388 4275 493
Size: 711 1184 1887 2702
Fragments arranged by size:
2702 1887 1184 711
BsaBI GATnn'nnATC
Cuts at: 6478 6478
Size: 6484
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3750 4291 4673 5308
Size: 53 83 186 936 2217 541 382 635
Cuts at: 5308 275
Size: 1451
Fragments arranged by size:
2217 1451 936 635 541 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 2199 3009 4917 5315 5589 5736 6147 2199
Size: 810 1908 398 274 147 411 2536
Fragments arranged by size:
2536 1908 810 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2198 2228 2672 2702 1178
Size: 30 165 30 795 30 444 30 4960
Fragments arranged by size:
4960 795 444 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2636 2639 2669 2693 1186
Size: 1450 3 30 24 4977
Fragments arranged by size:
4977 1450 30 24 3
BseYI C'CCAG_C
Cuts at: 2101 2853 3308 3344 3499 3585 5687 6183 2101
Size: 752 455 36 155 86 2102 496 2402
Fragments arranged by size:
2402 2102 752 496 455 155 86 36
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2078 1163
Size: 573 342 5569
Fragments arranged by size:
5569 573 342
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2961 3357 3934 4695 5723 1540
Size: 275 186 960 396 577 761 1028 2301
Fragments arranged by size:
2301 1028 960 761 577 396 275 186
BsiHKAI G_wGCw'C
Cuts at: 2631 4545 4630 5701 2631
Size: 1914 85 1071 3414
Fragments arranged by size:
3414 1914 1071 85
BsmI GAATG_Cn'
Cuts at: 3995 4088 3995
Size: 93 6391
BsmBI CGTCTCn'nnnn_
Cuts at: 3434 3434
Size: 6484
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2180 2280 2464 2631 2894 3215 3270
Size: 399 390 100 184 167 263 321 55
Cuts at: 3270 3309 3326 3427 3803 4545 4630 5701 1391
Size: 39 17 101 376 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 399 390 376 321 263
184 167 101 100 85 55 39 17
BspEI T'CCGG_A
Cuts at: 2199 3009 6147 2199
Size: 810 3138 2536
Fragments arranged by size:
3138 2536 810
BspHI T'CATG_A
Cuts at: 4373 6103 4373
Size: 1730 4754
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2337 2610 3421 3561 3839 844
Size: 1493 273 811 140 278 3489
Fragments arranged by size:
3489 1493 811 278 273 140
BsrBI CCG'CTC
Cuts at: 1394 1823 4371 1394
Size: 429 2548 3507
Fragments arranged by size:
3507 2548 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2421 4979 5153 65
Size: 1183 1173 2558 174 1396
Fragments arranged by size:
2558 1396 1183 1173 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2956 2960 3284 5128 5315 1700
Size: 110 1146 4 324 1844 187 2869
Fragments arranged by size:
2869 1844 1146 324 187 110 4
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6484
BssHII G'CGCG_C
Cuts at: 1925 2076 1925
Size: 151 6333
BssSI C'ACGA_G
Cuts at: 2231 2945 4544 5556 2231
Size: 714 1599 1012 3159
Fragments arranged by size:
3159 1599 1012 714
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6484
BstBI TT'CG_AA
Cuts at: 5288 5288
Size: 6484
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6484
BstXI CCAn_nnnn'nTGG
Cuts at: 3175 3175
Size: 6484
BstYI r'GATC_y
Cuts at: 1972 2194 2668 3075 3814 4181 4566 4583 6035
Size: 222 474 407 739 367 385 17 1452
Cuts at: 6035 6121 6133 6479 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1452 739 474 407 385 367 346
222 86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2379 3251 3519 3745 513
Size: 552 939 375 872 268 226 3252
Fragments arranged by size:
3252 939 872 552 375 268 226
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2722 487
Size: 683 56 1496 4249
Fragments arranged by size:
4249 1496 683 56
BtsI GCAGTG_nn'
Cuts at: 2147 3538 4000 4794 4814 2147
Size: 1391 462 794 20 3817
Fragments arranged by size:
3817 1391 794 462 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6484
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6449
DraI TTT'AAA
Cuts at: 3788 3788
Size: 6484
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 3101 5491 809
Size: 507 1785 2390 1802
Fragments arranged by size:
2390 1802 1785 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2958 3354 3600
Size: 54 1425 50 275 18 1128 396 246
Cuts at: 3600 3931 4820 6449 8
Size: 331 889 1629 43
Fragments arranged by size:
1629 1425 1128 889 396 331 275 246
54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2958 3354 3931 1537
Size: 275 1146 396 577 4090
Fragments arranged by size:
4090 1146 577 396 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3477 3666 4414 1353
Size: 2124 189 748 3423
Fragments arranged by size:
3423 2124 748 189
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3021 5071 5445 5591 387
Size: 1064 138 1432 2050 374 146 1280
Fragments arranged by size:
2050 1432 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2629 2629
Size: 6484
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2078 2768 2834 2886 3441 3448 3690
Size: 393 446 690 66 52 555 7 242
Cuts at: 3690 3755 4547 5144 5931 6199 1239
Size: 65 792 597 787 268 1524
Fragments arranged by size:
1524 792 787 690 597 555 446 393
268 242 66 65 52 7
EcoNI CCTnn'n_nnAGG
Cuts at: 2877 3089 2877
Size: 212 6272
EcoO109I rG'GnC_Cy
Cuts at: 3142 3149 3256 3365 3405 3778 3142
Size: 7 107 109 40 373 5848
Fragments arranged by size:
5848 373 109 107 40 7
EcoRI G'AATT_C
Cuts at: 3793 4171 3793
Size: 378 6106
FspI TGC'GCA
Cuts at: 4990 4990
Size: 6484
HaeII r_GCGC'y
Cuts at: 1678 2372 3315 3547 5631 1678
Size: 694 943 232 2084 2531
Fragments arranged by size:
2531 2084 943 694 232
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2399 2431 2672 2704 5125
Size: 32 135 32 827 32 241 32 2421
Cuts at: 5125 5157 5199 5231 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 2421 827 241 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2722 2941 3828 4074 669
Size: 988 281 784 219 887 246 3079
Fragments arranged by size:
3079 988 887 784 281 246 219
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6484
HpaI GTT'AAC
Cuts at: 4074 4074
Size: 6484
KpnI G_GTAC'C
Cuts at: 3809 3809
Size: 6484
MfeI C'AATT_G
Cuts at: 4083 4083
Size: 6484
MluI A'CGCG_T
Cuts at: 6360 6360
Size: 6484
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5598 5782 1305
Size: 4293 184 2007
Fragments arranged by size:
4293 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4662
Fragments arranged by size:
4662 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3698 4442 4801 4960 518
Size: 648 2532 744 359 159 2042
Fragments arranged by size:
2532 2042 744 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2167 3900 4577 5725 5970 1606
Size: 561 1733 677 1148 245 2120
Fragments arranged by size:
2120 1733 1148 677 561 245
NaeI GCC'GGC
Cuts at: 2958 2962 3286 2958
Size: 4 324 6156
Fragments arranged by size:
6156 324 4
NcoI C'CATG_G
Cuts at: 513 1065 2004 2379 3251 513
Size: 552 939 375 872 3746
Fragments arranged by size:
3746 939 872 552 375
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6484
NgoMIV G'CCGG_C
Cuts at: 2956 2960 3284 2956
Size: 4 324 6156
Fragments arranged by size:
6156 324 4
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6484
NotI GC'GGCC_GC
Cuts at: 3931 3931
Size: 6484
NspI r_CATG'y
Cuts at: 1132 1229 3842 5387 1132
Size: 97 2613 1545 2229
Fragments arranged by size:
2613 2229 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6484
PciI A'CATG_T
Cuts at: 5383 5383
Size: 6484
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2101 2356 1108
Size: 993 255 5236
Fragments arranged by size:
5236 993 255
PmeI GTTT'AAAC
Cuts at: 3788 3788
Size: 6484
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4553 4585 6066 6098 4553
Size: 32 1481 32 4939
Fragments arranged by size:
4939 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 3256 3405 3778 3256
Size: 149 373 5962
Fragments arranged by size:
5962 373 149
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6484
PsiI TTA'TAA
Cuts at: 4054 4054
Size: 6484
PstI C_TGCA'G
Cuts at: 830 2190 2265 2577 2676 3624 3836 830
Size: 1360 75 312 99 948 212 3478
Fragments arranged by size:
3478 1360 948 312 212 99 75
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6484
PvuII CAG'CTG
Cuts at: 2167 2167
Size: 6484
SacI G_AGCT'C
Cuts at: 2631 2631
Size: 6484
SalI G'TCGA_C
Cuts at: 1936 3826 1936
Size: 1890 4594
SanDI GG'GwC_CC
Cuts at: 3405 3778 3405
Size: 373 6111
SapI GCTCTTCn'nnn_
Cuts at: 3666 3666
Size: 6484
SbfI CC_TGCA'GG
Cuts at: 3836 3836
Size: 6484
ScaI AGT'ACT
Cuts at: 4732 6444 4732
Size: 1712 4772
SfcI C'TryA_G
Cuts at: 826 1045 2186 2261 2573 2672 3620 3832 4967
Size: 219 1141 75 312 99 948 212 1135
Cuts at: 4967 5648 5839 826
Size: 681 191 1471
Fragments arranged by size:
1471 1141 1135 948 681 312 219 212
191 99 75
SfiI GGCCn_nnn'nGGCC
Cuts at: 2966 2966
Size: 6484
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6484
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6484
SmaI CCC'GGG
Cuts at: 1872 2977 3147 3811 1872
Size: 1105 170 664 4545
Fragments arranged by size:
4545 1105 664 170
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2537 3626 4588 5489 5751 820
Size: 197 926 594 1089 962 901 262 1553
Fragments arranged by size:
1553 1089 962 926 901 594 262 197
SnaBI TAC'GTA
Cuts at: 493 4275 493
Size: 3782 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6484
SphI G_CATG'C
Cuts at: 1132 3842 1132
Size: 2710 3774
SspI AAT'ATT
Cuts at: 5 52 4408 5
Size: 47 4356 2081
Fragments arranged by size:
4356 2081 47
StuI AGG'CCT
Cuts at: 1517 3386 1517
Size: 1869 4615
StyI C'CwwG_G
Cuts at: 513 1065 2004 2121 2379 2563 3054 3251 3923
Size: 552 939 117 258 184 491 197 672
Cuts at: 3923 513
Size: 3074
Fragments arranged by size:
3074 939 672 552 491 258 197 184
117
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3241 4511 4528 4681 4866 5311 325
Size: 1255 1661 1270 17 153 185 445 1498
Fragments arranged by size:
1661 1498 1270 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4730 6442 96
Size: 274 80 33 51 1443 2753 1712 138
Fragments arranged by size:
2753 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2222 2254 2462 2494 3035 3067 3262 3294 6444
Size: 32 208 32 541 32 195 32 3150
Cuts at: 6444 6476 2222
Size: 32 2230
Fragments arranged by size:
3150 2230 541 208 195 32 32 32
32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6484
XbaI T'CTAG_A
Cuts at: 3820 3820
Size: 6484
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2011 3258 3416 2011
Size: 1247 158 5079
Fragments arranged by size:
5079 1247 158
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6484
XmaI C'CCGG_G
Cuts at: 1870 2975 3145 3809 1870
Size: 1105 170 664 4545
Fragments arranged by size:
4545 1105 664 170
XmnI GAAnn'nnTTC
Cuts at: 4613 4613
Size: 6484
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4292 276
Size: 53 83 186 936 2758 2468
Fragments arranged by size:
2758 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI
BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI
FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI
MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI
NgoMIV NheI NotI NspI PasI PciI PflMI PmeI
PpiI PpuMI PshAI PsiI PstI PvuI PvuII SacI
SalI SanDI SapI SbfI ScaI SfcI SfiI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AarI AloI ApaI AscI AvrII BaeI BbeI BfrBI
BplI BsiWI BstZ17I Bsu36I DraIII EcoRV FalI FseI
FspAI KasI NarI NruI NsiI PacI PfoI PmlI
PspOMI PspXI PsrI RsrII SacII SexAI SfoI SrfI
SwaI