Restriction map for
pFN21AB5389
(Circular) (Six-base) MAPSORT of: pFN21AB5389.seq Check: 4586 from: 1 to: 6142
pFN21AB5389 6142 bp
With 182 enzymes: SgfI *
October 20, 2009 10:35 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3952 278
Size: 53 83 186 936 2416 2468
Fragments arranged by size:
2468 2416 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3485 1937
Size: 1548 4594
Acc65I G'GTAC_C
Cuts at: 3463 3463
Size: 6142
AclI AA'CG_TT
Cuts at: 1563 2883 3441 4269 4642 1563
Size: 1320 558 828 373 3063
Fragments arranged by size:
3063 1320 828 558 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2600 2915 4205 5589 5857 1632
Size: 968 315 1290 1384 268 1917
Fragments arranged by size:
1917 1384 1290 968 315 268
AfeI AGC'GCT
Cuts at: 3137 3137
Size: 6142
AflII C'TTAA_G
Cuts at: 820 1017 2078 820
Size: 197 1061 4884
Fragments arranged by size:
4884 1061 197
AflIII A'CryG_T
Cuts at: 1203 3177 5041 6018 1203
Size: 1974 1864 977 1327
Fragments arranged by size:
1974 1864 1327 977
AgeI A'CCGG_T
Cuts at: 4973 4973
Size: 6142
AhdI GACnn_n'nnGTC
Cuts at: 4871 4871
Size: 6142
AleI CACnn'nnGTG
Cuts at: 1166 3176 1166
Size: 2010 4132
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2064 1184
Size: 880 5262
AlwNI CAG_nnn'CTG
Cuts at: 2805 2815 3885 5457 2805
Size: 10 1070 1572 3490
Fragments arranged by size:
3490 1572 1070 10
ApaI G_GGCC'C
Cuts at: 2026 2026
Size: 6142
ApaLI G'TGCA_C
Cuts at: 4199 5355 4199
Size: 1156 4986
ApoI r'AATT_y
Cuts at: 1493 2564 3451 3677 3829 5938 1493
Size: 1071 887 226 152 2109 1697
Fragments arranged by size:
2109 1697 1071 887 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4696 160
Size: 872 3664 1606
Fragments arranged by size:
3664 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6142
AvaI C'yCGr_G
Cuts at: 1870 1943 3467 1870
Size: 73 1524 4545
Fragments arranged by size:
4545 1524 73
BamHI G'GATC_C
Cuts at: 2251 3472 3839 2251
Size: 1221 367 4554
Fragments arranged by size:
4554 1221 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2651 3463 4918 618
Size: 325 217 627 864 812 1455 1842
Fragments arranged by size:
1842 1455 864 812 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2026 2394 2526 3063 3461 1391
Size: 635 368 132 537 398 4072
Fragments arranged by size:
4072 635 537 398 368 132
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2150 2844 928
Size: 971 251 694 4226
Fragments arranged by size:
4226 971 694 251
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4333 4367 1903
Size: 34 2396 34 3678
Fragments arranged by size:
3678 2396 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4034 5244 4034
Size: 1210 4932
BclI T'GATC_A
Cuts at: 1548 2199 1548
Size: 651 5491
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 3303 4753 136
Size: 107 122 71 631 605 1631 1450 1525
Fragments arranged by size:
1631 1525 1450 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6137 6137
Size: 6142
BlpI GC'TnA_GC
Cuts at: 2388 2462 3559 2388
Size: 74 1097 4971
Fragments arranged by size:
4971 1097 74
Bme1580I G_kGCm'C
Cuts at: 1790 2026 3187 4203 5359 1790
Size: 236 1161 1016 1156 2573
Fragments arranged by size:
2573 1161 1156 1016 236
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6142
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2173 2488 3873
Size: 416 53 83 399 24 756 315 1385
Cuts at: 3873 4831 442
Size: 958 1753
Fragments arranged by size:
1753 1385 958 756 416 399 315 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 2588 1056
Size: 1532 4610
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 4802 1239
Size: 3563 2579
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2672 2991 4267 5132 5430 2672
Size: 319 1276 865 298 3384
Fragments arranged by size:
3384 1276 865 319 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2903 3260 4805 882
Size: 617 1404 357 1545 2219
Fragments arranged by size:
2219 1545 1404 617 357
BsaAI yAC'GTr
Cuts at: 493 1204 3933 493
Size: 711 2729 2702
Fragments arranged by size:
2729 2702 711
BsaBI GATnn'nnATC
Cuts at: 6136 6136
Size: 6142
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3949 4331 4966 275
Size: 53 83 186 936 2416 382 635 1451
Fragments arranged by size:
2416 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 3279 4575 4973 5247 5394 5805 3279
Size: 1296 398 274 147 411 3616
Fragments arranged by size:
3616 1296 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2298 2328 2663 2693 1178
Size: 30 165 30 895 30 335 30 4627
Fragments arranged by size:
4627 895 335 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 6142
BseYI C'CCAG_C
Cuts at: 5345 5841 5345
Size: 496 5646
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2965 3260 1163
Size: 573 1229 295 4045
Fragments arranged by size:
4045 1229 573 295
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3592 4353 5381 1540
Size: 275 186 1591 761 1028 2301
Fragments arranged by size:
2301 1591 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2526 2681 3063 4203 4288 5359 2526
Size: 155 382 1140 85 1071 3309
Fragments arranged by size:
3309 1140 1071 382 155 85
BsmI GAATG_Cn'
Cuts at: 3653 3746 3653
Size: 93 6049
BsmBI CGTCTCn'nnnn_
Cuts at: 2949 2949
Size: 6142
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2026 2394 2526 2681 3063 3187 3461
Size: 399 236 368 132 155 382 124 274
Cuts at: 3461 4203 4288 5359 1391
Size: 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 399 382 368 274 236
155 132 124 85
BspEI T'CCGG_A
Cuts at: 5805 5805
Size: 6142
BspHI T'CATG_A
Cuts at: 2742 4031 5761 2742
Size: 1289 1730 3123
Fragments arranged by size:
3123 1730 1289
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2485 3282 3497 844
Size: 1641 797 215 3489
Fragments arranged by size:
3489 1641 797 215
BsrBI CCG'CTC
Cuts at: 1394 1823 4029 1394
Size: 429 2206 3507
Fragments arranged by size:
3507 2206 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2369 3000 4637 4811 65
Size: 1183 1121 631 1637 174 1396
Fragments arranged by size:
1637 1396 1183 1121 631 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4786 4973 1700
Size: 110 2976 187 2869
Fragments arranged by size:
2976 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6142
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6142
BssSI C'ACGA_G
Cuts at: 4202 5214 4202
Size: 1012 5130
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6142
BstBI TT'CG_AA
Cuts at: 4946 4946
Size: 6142
BstEII G'GTnAC_C
Cuts at: 1184 3166 1184
Size: 1982 4160
BstYI r'GATC_y
Cuts at: 1972 2251 3472 3839 4224 4241 5693 5779 5791
Size: 279 1221 367 385 17 1452 86 12
Cuts at: 5791 6137 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 1221 385 367 346 279 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2331 3187 3237 513
Size: 552 939 327 856 50 3418
Fragments arranged by size:
3418 939 856 552 327 50
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5403
Fragments arranged by size:
5403 683 56
BtsI GCAGTG_nn'
Cuts at: 3132 3327 3658 4452 4472 3132
Size: 195 331 794 20 4802
Fragments arranged by size:
4802 794 331 195 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6142
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2214 2249 529
Size: 35 1650 35 4422
Fragments arranged by size:
4422 1650 35 35
DraI TTT'AAA
Cuts at: 3446 3446
Size: 6142
DraIII CAC_nnn'GTG
Cuts at: 3082 3082
Size: 6142
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5149 809
Size: 507 3833 1802
Fragments arranged by size:
3833 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2007 2454 3589
Size: 54 1425 50 275 18 177 447 1135
Cuts at: 3589 4478 6107 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 1135 889 447 275 177 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3589 1537
Size: 275 1777 4090
Fragments arranged by size:
4090 1777 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2079 2151 2283 2901 4072 1353
Size: 726 72 132 618 1171 3423
Fragments arranged by size:
3423 1171 726 618 132 72
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2815 4729 5103 5249 387
Size: 1064 138 1226 1914 374 146 1280
Fragments arranged by size:
1914 1280 1226 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2524 3061 2524
Size: 537 5605
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2600 2915 4205 4802 5589 5857 1239
Size: 393 968 315 1290 597 787 268 1524
Fragments arranged by size:
1524 1290 968 787 597 393 315 268
EcoO109I rG'GnC_Cy
Cuts at: 2023 3313 2023
Size: 1290 4852
EcoRI G'AATT_C
Cuts at: 3451 3829 3451
Size: 378 5764
FspI TGC'GCA
Cuts at: 4648 4648
Size: 6142
HaeII r_GCGC'y
Cuts at: 1678 3139 5289 1678
Size: 1461 2150 2531
Fragments arranged by size:
2531 2150 1461
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2243 2275 2663 2695 2737
Size: 32 135 32 671 32 388 32 42
Cuts at: 2737 2769 4783 4815 4857 4889 1373
Size: 32 2014 32 42 32 2626
Fragments arranged by size:
2626 2014 671 388 135 42 42 32
32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3486 3732 669
Size: 988 281 1548 246 3079
Fragments arranged by size:
3079 1548 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6142
HpaI GTT'AAC
Cuts at: 3732 3732
Size: 6142
KpnI G_GTAC'C
Cuts at: 3467 3467
Size: 6142
MfeI C'AATT_G
Cuts at: 3741 3741
Size: 6142
MluI A'CGCG_T
Cuts at: 6018 6018
Size: 6142
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5256 5440 1305
Size: 3951 184 2007
Fragments arranged by size:
3951 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2009 2456 10
Size: 54 1425 343 177 447 3696
Fragments arranged by size:
3696 1425 447 343 177 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3176 4100 4459 4618 518
Size: 648 2010 924 359 159 2042
Fragments arranged by size:
2042 2010 924 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2248 2441 3189 3558 4235 5383 5628 1606
Size: 642 193 748 369 677 1148 245 2120
Fragments arranged by size:
2120 1148 748 677 642 369 245 193
NcoI C'CATG_G
Cuts at: 513 1065 2004 2331 513
Size: 552 939 327 4324
Fragments arranged by size:
4324 939 552 327
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6142
NheI G'CTAG_C
Cuts at: 1052 2584 1052
Size: 1532 4610
NotI GC'GGCC_GC
Cuts at: 3589 3589
Size: 6142
NspI r_CATG'y
Cuts at: 1132 1229 3500 5045 1132
Size: 97 2271 1545 2229
Fragments arranged by size:
2271 2229 1545 97
PasI CC'CwG_GG
Cuts at: 1348 2657 1348
Size: 1309 4833
PciI A'CATG_T
Cuts at: 5041 5041
Size: 6142
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2866 3082 1108
Size: 1758 216 4168
Fragments arranged by size:
4168 1758 216
PfoI T'CCnGG_A
Cuts at: 3369 3369
Size: 6142
PmeI GTTT'AAAC
Cuts at: 3446 3446
Size: 6142
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4211 4243 5724 5756 4211
Size: 32 1481 32 4597
Fragments arranged by size:
4597 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6142
PsiI TTA'TAA
Cuts at: 3712 3712
Size: 6142
PspOMI G'GGCC_C
Cuts at: 2022 2022
Size: 6142
PstI C_TGCA'G
Cuts at: 830 2247 3127 3494 830
Size: 1417 880 367 3478
Fragments arranged by size:
3478 1417 880 367
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6142
PvuII CAG'CTG
Cuts at: 2441 2441
Size: 6142
SacI G_AGCT'C
Cuts at: 2526 3063 2526
Size: 537 5605
SacII CC_GC'GG
Cuts at: 3190 3190
Size: 6142
SalI G'TCGA_C
Cuts at: 1936 3484 1936
Size: 1548 4594
SapI GCTCTTCn'nnn_
Cuts at: 2283 2283
Size: 6142
SbfI CC_TGCA'GG
Cuts at: 3494 3494
Size: 6142
ScaI AGT'ACT
Cuts at: 2933 4390 6102 2933
Size: 1457 1712 2973
Fragments arranged by size:
2973 1712 1457
SexAI A'CCwGG_T
Cuts at: 3078 3078
Size: 6142
SfcI C'TryA_G
Cuts at: 826 1045 2243 3123 3490 4625 5306 5497 826
Size: 219 1198 880 367 1135 681 191 1471
Fragments arranged by size:
1471 1198 1135 880 681 367 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6142
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6142
SmaI CCC'GGG
Cuts at: 1872 3469 1872
Size: 1597 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2078 2687 3006 4246 5147 5409
Size: 197 926 135 609 319 1240 901 262
Cuts at: 5409 820
Size: 1553
Fragments arranged by size:
1553 1240 926 901 609 319 262 197
135
SnaBI TAC'GTA
Cuts at: 493 3933 493
Size: 3440 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6142
SphI G_CATG'C
Cuts at: 1132 3500 1132
Size: 2368 3774
SspI AAT'ATT
Cuts at: 5 52 4066 5
Size: 47 4014 2081
Fragments arranged by size:
4014 2081 47
StuI AGG'CCT
Cuts at: 1517 2972 1517
Size: 1455 4687
StyI C'CwwG_G
Cuts at: 513 1065 2004 2317 2331 3581 513
Size: 552 939 313 14 1250 3074
Fragments arranged by size:
3074 1250 939 552 313 14
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 4169 4186 4339 4524 4969 325
Size: 1255 2589 17 153 185 445 1498
Fragments arranged by size:
2589 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2268 2931 4388
Size: 274 80 33 51 1443 291 663 1457
Cuts at: 4388 6100 96
Size: 1712 138
Fragments arranged by size:
1712 1457 1443 663 291 274 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2298 2330 6102 6134 2298
Size: 32 3772 32 2306
Fragments arranged by size:
3772 2306 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6142
XbaI T'CTAG_A
Cuts at: 3478 3478
Size: 6142
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2420 2420
Size: 6142
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6142
XmaI C'CCGG_G
Cuts at: 1870 3467 1870
Size: 1597 4545
XmnI GAAnn'nnTTC
Cuts at: 2292 4271 2292
Size: 1979 4163
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3950 276
Size: 53 83 186 936 2416 2468
Fragments arranged by size:
2468 2416 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI
BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FspI HaeII
Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI
MscI MslI MspA1I NcoI NdeI NheI NotI NspI
PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI
PspOMI PstI PvuI PvuII SacI SacII SalI SapI
SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AloI AscI AvrII BaeI BbeI BbvCI BfrBI
BplI Bpu10I BsiWI BstXI BstZ17I Bsu36I EcoNI EcoRV
FalI FseI FspAI KasI NaeI NarI NgoMIV NruI
NsiI PacI PmlI PpuMI PspXI PsrI RsrII SanDI
SfiI SfoI SrfI SwaI