Restriction map for
pFN21AB5387
(Circular) (Six-base) MAPSORT of: pFN21AB5387.seq Check: 2625 from: 1 to: 6334
pFN21AB5387 6334 bp
With 182 enzymes: SgfI *
October 20, 2009 10:35 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4144 278
Size: 53 83 186 936 2608 2468
Fragments arranged by size:
2608 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3677 1937
Size: 1740 4594
Acc65I G'GTAC_C
Cuts at: 3655 3655
Size: 6334
AclI AA'CG_TT
Cuts at: 1563 4461 4834 1563
Size: 2898 373 3063
Fragments arranged by size:
3063 2898 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 4397 5781 6049 1632
Size: 2765 1384 268 1917
Fragments arranged by size:
2765 1917 1384 268
AfeI AGC'GCT
Cuts at: 3524 3524
Size: 6334
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6137
AflIII A'CryG_T
Cuts at: 1203 5233 6210 1203
Size: 4030 977 1327
Fragments arranged by size:
4030 1327 977
AgeI A'CCGG_T
Cuts at: 5165 5165
Size: 6334
AhdI GACnn_n'nnGTC
Cuts at: 5063 5063
Size: 6334
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6334
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 3621 1184
Size: 2437 3897
AlwNI CAG_nnn'CTG
Cuts at: 2647 4077 5649 2647
Size: 1430 1572 3332
Fragments arranged by size:
3332 1572 1430
ApaI G_GGCC'C
Cuts at: 2776 2776
Size: 6334
ApaLI G'TGCA_C
Cuts at: 3095 4391 5547 3095
Size: 1296 1156 3882
Fragments arranged by size:
3882 1296 1156
ApoI r'AATT_y
Cuts at: 1493 2168 3643 3869 4021 6130 1493
Size: 675 1475 226 152 2109 1697
Fragments arranged by size:
2109 1697 1475 675 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4888 160
Size: 872 3856 1606
Fragments arranged by size:
3856 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6334
AvaI C'yCGr_G
Cuts at: 1870 1943 2574 3659 1870
Size: 73 631 1085 4545
Fragments arranged by size:
4545 1085 631 73
BamHI G'GATC_C
Cuts at: 2620 3664 4031 2620
Size: 1044 367 4923
Fragments arranged by size:
4923 1044 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2206 2668 3193 3655 5110
Size: 325 217 627 419 462 525 462 1455
Cuts at: 5110 618
Size: 1842
Fragments arranged by size:
1842 1455 627 525 462 462 419 325
217
BanII G_rGCy'C
Cuts at: 1391 2156 2433 2702 2776 3588 3653 1391
Size: 765 277 269 74 812 65 4072
Fragments arranged by size:
4072 812 765 277 269 74 65
BbeI G_GCGC'C
Cuts at: 2672 2672
Size: 6334
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 5363
BbvCI CC'TCA_GC
Cuts at: 2447 2829 2447
Size: 382 5952
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4525 4559 1903
Size: 34 2588 34 3678
Fragments arranged by size:
3678 2588 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4226 5436 4226
Size: 1210 5124
BclI T'GATC_A
Cuts at: 1548 2085 2200 1548
Size: 537 115 5682
Fragments arranged by size:
5682 537 115
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4945 136
Size: 107 122 71 631 605 3273 1525
Fragments arranged by size:
3273 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6329 6329
Size: 6334
BlpI GC'TnA_GC
Cuts at: 3751 3751
Size: 6334
Bme1580I G_kGCm'C
Cuts at: 1790 2096 2776 3099 4395 5551 1790
Size: 306 680 323 1296 1156 2573
Fragments arranged by size:
2573 1296 1156 680 323 306
BmgBI CAC'GTC
Cuts at: 1318 2287 3329 1318
Size: 969 1042 4323
Fragments arranged by size:
4323 1042 969
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2334 3118 3547
Size: 416 53 83 399 24 917 784 429
Cuts at: 3547 4065 5023 442
Size: 518 958 1753
Fragments arranged by size:
1753 958 917 784 518 429 416 399
83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6334
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2140 2298 2445 2999 4994 1239
Size: 901 158 147 554 1995 2579
Fragments arranged by size:
2579 1995 901 554 158 147
Bpu10I CC'TnA_GC
Cuts at: 2122 2447 2829 2122
Size: 325 382 5627
Fragments arranged by size:
5627 382 325
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4459 5324 5622 4459
Size: 865 298 5171
Fragments arranged by size:
5171 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2039 2274 3436 3487 4997 882
Size: 617 540 235 1162 51 1510 2219
Fragments arranged by size:
2219 1510 1162 617 540 235 51
BsaAI yAC'GTr
Cuts at: 493 1204 3232 4125 493
Size: 711 2028 893 2702
Fragments arranged by size:
2702 2028 893 711
BsaBI GATnn'nnATC
Cuts at: 6328 6328
Size: 6334
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2669 4141 4523 5158
Size: 53 83 186 936 1136 1472 382 635
Cuts at: 5158 275
Size: 1451
Fragments arranged by size:
1472 1451 1136 936 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 3501 4767 5165 5439 5586 5997 3501
Size: 1266 398 274 147 411 3838
Fragments arranged by size:
3838 1266 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2271 2301 2526 2556 1178
Size: 30 165 30 868 30 225 30 4956
Fragments arranged by size:
4956 868 225 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2456 2477 2562 2692 3017 3596 1186
Size: 1270 21 85 130 325 579 3924
Fragments arranged by size:
3924 1270 579 325 130 85 21
BseYI C'CCAG_C
Cuts at: 2271 3078 5537 6033 2271
Size: 807 2459 496 2572
Fragments arranged by size:
2572 2459 807 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2988 1163
Size: 573 1252 4509
Fragments arranged by size:
4509 1252 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2839 3055 3784 4545 5573 1540
Size: 275 186 838 216 729 761 1028 2301
Fragments arranged by size:
2301 1028 838 761 729 275 216 186
BsiHKAI G_wGCw'C
Cuts at: 2433 3099 3588 4395 4480 5551 2433
Size: 666 489 807 85 1071 3216
Fragments arranged by size:
3216 1071 807 666 489 85
BsmI GAATG_Cn'
Cuts at: 2895 3845 3938 2895
Size: 950 93 5291
Fragments arranged by size:
5291 950 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2096 2156 2433 2702 2776 3099 3588
Size: 399 306 60 277 269 74 323 489
Cuts at: 3588 3653 4395 4480 5551 1391
Size: 65 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 489 399 323 306 277
269 85 74 65 60
BspEI T'CCGG_A
Cuts at: 5997 5997
Size: 6334
BspHI T'CATG_A
Cuts at: 3150 4223 5953 3150
Size: 1073 1730 3531
Fragments arranged by size:
3531 1730 1073
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3007 3589 3689 844
Size: 2163 582 100 3489
Fragments arranged by size:
3489 2163 582 100
BsrBI CCG'CTC
Cuts at: 1394 1823 2121 4221 1394
Size: 429 298 2100 3507
Fragments arranged by size:
3507 2100 429 298
BsrDI GCAATG_nn'
Cuts at: 65 1248 2880 4829 5003 65
Size: 1183 1632 1949 174 1396
Fragments arranged by size:
1949 1632 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2891 3045 3108 4978 5165 1700
Size: 110 1081 154 63 1870 187 2869
Fragments arranged by size:
2869 1870 1081 187 154 110 63
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6334
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6334
BssSI C'ACGA_G
Cuts at: 2852 3534 4394 5406 2852
Size: 682 860 1012 3780
Fragments arranged by size:
3780 1012 860 682
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6334
BstBI TT'CG_AA
Cuts at: 5138 5138
Size: 6334
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6334
BstXI CCAn_nnnn'nTGG
Cuts at: 2011 3322 2011
Size: 1311 5023
BstYI r'GATC_y
Cuts at: 1972 2620 3664 4031 4416 4433 5885 5971 5983
Size: 648 1044 367 385 17 1452 86 12
Cuts at: 5983 6329 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 1044 648 385 367 346 86
17 12
Bsu36I CC'TnA_GG
Cuts at: 2704 2704
Size: 6334
BtgI C'CryG_G
Cuts at: 513 1065 2004 3189 513
Size: 552 939 1185 3658
Fragments arranged by size:
3658 1185 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5595
Fragments arranged by size:
5595 683 56
BtsI GCAGTG_nn'
Cuts at: 2534 3005 3315 3850 4644 4664 2534
Size: 471 310 535 794 20 4204
Fragments arranged by size:
4204 794 535 471 310 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6334
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6299
DraI TTT'AAA
Cuts at: 3638 3638
Size: 6334
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5341 809
Size: 507 4025 1802
Fragments arranged by size:
4025 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2013 2631 3781
Size: 54 1425 50 275 18 183 618 1150
Cuts at: 3781 4670 6299 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 1150 889 618 275 183 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3781 1537
Size: 275 1969 4090
Fragments arranged by size:
4090 1969 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3411 4264 1353
Size: 2058 853 3423
Fragments arranged by size:
3423 2058 853
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4921 5295 5441 387
Size: 1064 138 3332 374 146 1280
Fragments arranged by size:
3332 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2431 3586 2431
Size: 1155 5179
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2140 2298 2445 2999 4397 4994 5781
Size: 393 508 158 147 554 1398 597 787
Cuts at: 5781 6049 1239
Size: 268 1524
Fragments arranged by size:
1524 1398 787 597 554 508 393 268
158 147
EcoO109I rG'GnC_Cy
Cuts at: 2772 2773 2772
Size: 1 6333
EcoRI G'AATT_C
Cuts at: 3643 4021 3643
Size: 378 5956
FspI TGC'GCA
Cuts at: 2321 4840 2321
Size: 2519 3815
HaeII r_GCGC'y
Cuts at: 1678 2672 2959 3526 5481 1678
Size: 994 287 567 1955 2531
Fragments arranged by size:
2531 1955 994 567 287
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2050 2082 2271 2303 2399
Size: 32 135 32 478 32 189 32 96
Cuts at: 2399 2431 2605 2637 4975 5007 5049 5081 1373
Size: 32 174 32 2338 32 42 32 2626
Fragments arranged by size:
2626 2338 478 189 174 135 96 42
32 32 32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3678 3924 669
Size: 988 281 1740 246 3079
Fragments arranged by size:
3079 1740 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6334
HpaI GTT'AAC
Cuts at: 3924 3924
Size: 6334
KasI G'GCGC_C
Cuts at: 2668 2668
Size: 6334
KpnI G_GTAC'C
Cuts at: 3659 3659
Size: 6334
MfeI C'AATT_G
Cuts at: 3933 3933
Size: 6334
MluI A'CGCG_T
Cuts at: 6210 6210
Size: 6334
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2356 5448 5632 1305
Size: 1051 3092 184 2007
Fragments arranged by size:
3092 2007 1051 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2015 2633 10
Size: 54 1425 343 183 618 3711
Fragments arranged by size:
3711 1425 618 343 183 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2009 2075 2520 3167 4292 4651 4810
Size: 648 843 66 445 647 1125 359 159
Cuts at: 4810 518
Size: 2042
Fragments arranged by size:
2042 1125 843 648 647 445 359 159
66
MspA1I CmG'CkG
Cuts at: 1606 2726 2919 3750 4427 5575 5820 1606
Size: 1120 193 831 677 1148 245 2120
Fragments arranged by size:
2120 1148 1120 831 677 245 193
NaeI GCC'GGC
Cuts at: 2893 3110 2893
Size: 217 6117
NarI GG'CG_CC
Cuts at: 2669 2669
Size: 6334
NcoI C'CATG_G
Cuts at: 513 1065 2004 3189 513
Size: 552 939 1185 3658
Fragments arranged by size:
3658 1185 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6334
NgoMIV G'CCGG_C
Cuts at: 2891 3108 2891
Size: 217 6117
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6334
NotI GC'GGCC_GC
Cuts at: 3781 3781
Size: 6334
NspI r_CATG'y
Cuts at: 1132 1229 3692 5237 1132
Size: 97 2463 1545 2229
Fragments arranged by size:
2463 2229 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6334
PciI A'CATG_T
Cuts at: 5233 5233
Size: 6334
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6334
PfoI T'CCnGG_A
Cuts at: 2172 3611 2172
Size: 1439 4895
PmeI GTTT'AAAC
Cuts at: 3638 3638
Size: 6334
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4403 4435 5916 5948 4403
Size: 32 1481 32 4789
Fragments arranged by size:
4789 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6334
PsiI TTA'TAA
Cuts at: 3904 3904
Size: 6334
PspOMI G'GGCC_C
Cuts at: 2772 2772
Size: 6334
PspXI vC'TCGA_Gb
Cuts at: 2574 2574
Size: 6334
PstI C_TGCA'G
Cuts at: 830 2145 3686 830
Size: 1315 1541 3478
Fragments arranged by size:
3478 1541 1315
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6334
PvuII CAG'CTG
Cuts at: 2726 2726
Size: 6334
SacI G_AGCT'C
Cuts at: 2433 3588 2433
Size: 1155 5179
SalI G'TCGA_C
Cuts at: 1936 3676 1936
Size: 1740 4594
SbfI CC_TGCA'GG
Cuts at: 3686 3686
Size: 6334
ScaI AGT'ACT
Cuts at: 4582 6294 4582
Size: 1712 4622
SfcI C'TryA_G
Cuts at: 826 1045 2141 3682 4817 5498 5689 826
Size: 219 1096 1541 1135 681 191 1471
Fragments arranged by size:
1541 1471 1135 1096 681 219 191
SfoI GGC'GCC
Cuts at: 2670 2670
Size: 6334
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6334
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6334
SmaI CCC'GGG
Cuts at: 1872 3661 1872
Size: 1789 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2574 4438 5339 5601 820
Size: 197 926 631 1864 901 262 1553
Fragments arranged by size:
1864 1553 926 901 631 262 197
SnaBI TAC'GTA
Cuts at: 493 4125 493
Size: 3632 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6334
SphI G_CATG'C
Cuts at: 1132 3692 1132
Size: 2560 3774
SspI AAT'ATT
Cuts at: 5 52 4258 5
Size: 47 4206 2081
Fragments arranged by size:
4206 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6334
StyI C'CwwG_G
Cuts at: 513 1065 2004 2886 3189 3773 513
Size: 552 939 882 303 584 3074
Fragments arranged by size:
3074 939 882 584 552 303
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3069 4361 4378 4531 4716 5161 325
Size: 1255 1489 1292 17 153 185 445 1498
Fragments arranged by size:
1498 1489 1292 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2682 4580 6292
Size: 274 80 33 51 1443 705 1898 1712
Cuts at: 6292 96
Size: 138
Fragments arranged by size:
1898 1712 1443 705 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2526 2558 6294 6326 2526
Size: 32 3736 32 2534
Fragments arranged by size:
3736 2534 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 2285 1316
Size: 969 5365
XbaI T'CTAG_A
Cuts at: 3670 3670
Size: 6334
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3382 3382
Size: 6334
XhoI C'TCGA_G
Cuts at: 1943 2574 1943
Size: 631 5703
XmaI C'CCGG_G
Cuts at: 1870 3659 1870
Size: 1789 4545
XmnI GAAnn'nnTTC
Cuts at: 4463 4463
Size: 6334
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4142 276
Size: 53 83 186 936 2608 2468
Fragments arranged by size:
2608 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI
BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI
BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I
EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI
KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI
NarI NcoI NdeI NgoMIV NheI NotI NspI PasI
PciI PflMI PfoI PmeI PpiI PshAI PsiI PspOMI
PspXI PstI PvuI PvuII SacI SalI SbfI ScaI
SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AloI AscI AvrII BaeI BfrBI BplI BsiWI
BsmBI BstZ17I DraIII EcoNI EcoRV FalI FseI FspAI
NruI NsiI PacI PmlI PpuMI PsrI RsrII SacII
SanDI SapI SexAI SfiI SrfI SwaI