Restriction map for
pFN21AB5377
(Circular) (Six-base) MAPSORT of: pFN21AB5377.seq Check: 9365 from: 1 to: 5725
pFN21AB5377 5725 bp
With 182 enzymes: SgfI *
January 16, 2009 15:25 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3535 278
Size: 53 83 186 936 1999 2468
Fragments arranged by size:
2468 1999 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3068 1937
Size: 1131 4594
Acc65I G'GTAC_C
Cuts at: 2264 3046 2264
Size: 782 4943
AclI AA'CG_TT
Cuts at: 1563 3852 4225 1563
Size: 2289 373 3063
Fragments arranged by size:
3063 2289 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3788 5172 5440 1632
Size: 2156 1384 268 1917
Fragments arranged by size:
2156 1917 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5528
AflIII A'CryG_T
Cuts at: 1203 4624 5601 1203
Size: 3421 977 1327
Fragments arranged by size:
3421 1327 977
AgeI A'CCGG_T
Cuts at: 4556 4556
Size: 5725
AhdI GACnn_n'nnGTC
Cuts at: 4454 4454
Size: 5725
AleI CACnn'nnGTG
Cuts at: 1166 2432 1166
Size: 1266 4459
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5725
AlwNI CAG_nnn'CTG
Cuts at: 2089 2538 3468 5040 2089
Size: 449 930 1572 2774
Fragments arranged by size:
2774 1572 930 449
ApaLI G'TGCA_C
Cuts at: 3782 4938 3782
Size: 1156 4569
ApoI r'AATT_y
Cuts at: 1493 3034 3260 3412 5521 1493
Size: 1541 226 152 2109 1697
Fragments arranged by size:
2109 1697 1541 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4279 160
Size: 872 3247 1606
Fragments arranged by size:
3247 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5725
AvaI C'yCGr_G
Cuts at: 1870 1943 2927 3050 1870
Size: 73 984 123 4545
Fragments arranged by size:
4545 984 123 73
BamHI G'GATC_C
Cuts at: 3055 3422 3055
Size: 367 5358
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2123 2255 2264 3046 4501
Size: 325 217 627 336 132 9 782 1455
Cuts at: 4501 618
Size: 1842
Fragments arranged by size:
1842 1455 782 627 336 325 217 132
9
BanII G_rGCy'C
Cuts at: 1391 2121 2469 3044 1391
Size: 730 348 575 4072
Fragments arranged by size:
4072 730 575 348
BbeI G_GCGC'C
Cuts at: 2127 2127
Size: 5725
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2610 928
Size: 971 711 4043
Fragments arranged by size:
4043 971 711
BbvCI CC'TCA_GC
Cuts at: 2151 2151
Size: 5725
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3916 3950 1903
Size: 34 1979 34 3678
Fragments arranged by size:
3678 1979 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3617 4827 3617
Size: 1210 4515
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5725
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2238 4336 136
Size: 107 122 71 631 605 566 2098 1525
Fragments arranged by size:
2098 1525 631 605 566 122 107 71
BglII A'GATC_T
Cuts at: 2190 2526 5720 2190
Size: 336 3194 2195
Fragments arranged by size:
3194 2195 336
BlpI GC'TnA_GC
Cuts at: 2168 3142 2168
Size: 974 4751
Bme1580I G_kGCm'C
Cuts at: 1790 3786 4942 1790
Size: 1996 1156 2573
Fragments arranged by size:
2573 1996 1156
BmgBI CAC'GTC
Cuts at: 1318 2164 1318
Size: 846 4879
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2229 2486 3456
Size: 416 53 83 399 24 812 257 970
Cuts at: 3456 4414 442
Size: 958 1753
Fragments arranged by size:
1753 970 958 812 416 399 257 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5725
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2924 2924
Size: 5725
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2919 4385 1239
Size: 1680 1466 2579
Fragments arranged by size:
2579 1680 1466
Bpu10I CC'TnA_GC
Cuts at: 2151 2151
Size: 5725
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3026 3850 4715 5013 3026
Size: 824 865 298 3738
Fragments arranged by size:
3738 865 824 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2286 2670 4388 882
Size: 617 787 384 1718 2219
Fragments arranged by size:
2219 1718 787 617 384
BsaAI yAC'GTr
Cuts at: 493 1204 3516 493
Size: 711 2312 2702
Fragments arranged by size:
2702 2312 711
BsaBI GATnn'nnATC
Cuts at: 2555 5719 2555
Size: 3164 2561
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2124 3532 3914 4549
Size: 53 83 186 936 591 1408 382 635
Cuts at: 4549 275
Size: 1451
Fragments arranged by size:
1451 1408 936 635 591 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 4158 4556 4830 4977 5388 4158
Size: 398 274 147 411 4495
Fragments arranged by size:
4495 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2669 2699 2786 2816 1178
Size: 30 165 30 1266 30 87 30 4087
Fragments arranged by size:
4087 1266 165 87 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2072 2144 1186
Size: 886 72 4767
Fragments arranged by size:
4767 886 72
BseYI C'CCAG_C
Cuts at: 4928 5424 4928
Size: 496 5229
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2794 1163
Size: 573 1058 4094
Fragments arranged by size:
4094 1058 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2200 3175 3936 4964 1540
Size: 275 186 199 975 761 1028 2301
Fragments arranged by size:
2301 1028 975 761 275 199 186
BsiHKAI G_wGCw'C
Cuts at: 2169 3786 3871 4942 2169
Size: 1617 85 1071 2952
Fragments arranged by size:
2952 1617 1071 85
BsmI GAATG_Cn'
Cuts at: 2592 3236 3329 2592
Size: 644 93 4988
Fragments arranged by size:
4988 644 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2121 2169 2469 3044 3786 3871 4942
Size: 399 331 48 300 575 742 85 1071
Cuts at: 4942 1391
Size: 2174
Fragments arranged by size:
2174 1071 742 575 399 331 300 85
48
BspEI T'CCGG_A
Cuts at: 5388 5388
Size: 5725
BspHI T'CATG_A
Cuts at: 3614 5344 3614
Size: 1730 3995
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3001 3080 844
Size: 2157 79 3489
Fragments arranged by size:
3489 2157 79
BsrBI CCG'CTC
Cuts at: 1394 1823 2448 2454 3612 1394
Size: 429 625 6 1158 3507
Fragments arranged by size:
3507 1158 625 429 6
BsrDI GCAATG_nn'
Cuts at: 65 1248 4220 4394 65
Size: 1183 2972 174 1396
Fragments arranged by size:
2972 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4369 4556 1700
Size: 110 2559 187 2869
Fragments arranged by size:
2869 2559 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5725
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5725
BssSI C'ACGA_G
Cuts at: 3785 4797 3785
Size: 1012 4713
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5725
BstBI TT'CG_AA
Cuts at: 2596 4529 2596
Size: 1933 3792
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5725
BstYI r'GATC_y
Cuts at: 1972 2190 2485 2526 3055 3422 3807 3824 5276
Size: 218 295 41 529 367 385 17 1452
Cuts at: 5276 5362 5374 5720 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1452 529 385 367 346 295 218
86 41 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2041 2287 513
Size: 552 939 37 246 3951
Fragments arranged by size:
3951 939 552 246 37
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4986
Fragments arranged by size:
4986 683 56
BtsI GCAGTG_nn'
Cuts at: 3241 4035 4055 3241
Size: 794 20 4911
Fragments arranged by size:
4911 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5725
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5690
DraI TTT'AAA
Cuts at: 2721 3029 2721
Size: 308 5417
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2510 4732 809
Size: 507 1194 2222 1802
Fragments arranged by size:
2222 1802 1194 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2443 3172 4061
Size: 54 1425 50 275 18 613 729 889
Cuts at: 4061 5690 8
Size: 1629 43
Fragments arranged by size:
1629 1425 889 729 613 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3172 1537
Size: 275 1360 4090
Fragments arranged by size:
4090 1360 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2517 3655 1353
Size: 1164 1138 3423
Fragments arranged by size:
3423 1164 1138
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4312 4686 4832 387
Size: 1064 138 2723 374 146 1280
Fragments arranged by size:
2723 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2919 3788 4385 5172 5440 1239
Size: 393 1287 869 597 787 268 1524
Fragments arranged by size:
1524 1287 869 787 597 393 268
EcoNI CCTnn'n_nnAGG
Cuts at: 2792 2901 2792
Size: 109 5616
EcoO109I rG'GnC_Cy
Cuts at: 2941 2941
Size: 5725
EcoRI G'AATT_C
Cuts at: 3034 3412 3034
Size: 378 5347
EcoRV GAT'ATC
Cuts at: 2707 2707
Size: 5725
FspI TGC'GCA
Cuts at: 4231 4231
Size: 5725
HaeII r_GCGC'y
Cuts at: 1678 2090 2127 4872 1678
Size: 412 37 2745 2531
Fragments arranged by size:
2745 2531 412 37
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2381 2413 2669 2701 4366
Size: 32 135 32 809 32 256 32 1665
Cuts at: 4366 4398 4440 4472 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1665 809 256 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3069 3315 669
Size: 988 281 1131 246 3079
Fragments arranged by size:
3079 1131 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5725
HpaI GTT'AAC
Cuts at: 3315 3315
Size: 5725
KasI G'GCGC_C
Cuts at: 2123 2123
Size: 5725
KpnI G_GTAC'C
Cuts at: 2268 3050 2268
Size: 782 4943
MfeI C'AATT_G
Cuts at: 3324 3324
Size: 5725
MluI A'CGCG_T
Cuts at: 5601 5601
Size: 5725
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2031 2712 4839 5023 1305
Size: 726 681 2127 184 2007
Fragments arranged by size:
2127 2007 726 681 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3903
Fragments arranged by size:
3903 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2432 2624 3683 4042 4201 518
Size: 648 1266 192 1059 359 159 2042
Fragments arranged by size:
2042 1266 1059 648 359 192 159
MspA1I CmG'CkG
Cuts at: 1606 2043 2359 2535 2702 3141 3818 4966 5211
Size: 437 316 176 167 439 677 1148 245
Cuts at: 5211 1606
Size: 2120
Fragments arranged by size:
2120 1148 677 439 437 316 245 176
167
NarI GG'CG_CC
Cuts at: 2124 2124
Size: 5725
NcoI C'CATG_G
Cuts at: 513 1065 2004 2287 513
Size: 552 939 283 3951
Fragments arranged by size:
3951 939 552 283
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5725
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5725
NotI GC'GGCC_GC
Cuts at: 3172 3172
Size: 5725
NspI r_CATG'y
Cuts at: 1132 1229 2884 3083 4628 1132
Size: 97 1655 199 1545 2229
Fragments arranged by size:
2229 1655 1545 199 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5725
PciI A'CATG_T
Cuts at: 4624 4624
Size: 5725
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2029 2399 1108
Size: 921 370 4434
Fragments arranged by size:
4434 921 370
PfoI T'CCnGG_A
Cuts at: 2790 2896 2790
Size: 106 5619
PmeI GTTT'AAAC
Cuts at: 3029 3029
Size: 5725
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2120 2152 3794 3826 5307 5339 2120
Size: 32 1642 32 1481 32 2506
Fragments arranged by size:
2506 1642 1481 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 2941 2941
Size: 5725
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5725
PsiI TTA'TAA
Cuts at: 3295 3295
Size: 5725
PstI C_TGCA'G
Cuts at: 830 3077 830
Size: 2247 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5725
PvuII CAG'CTG
Cuts at: 2702 2702
Size: 5725
SacII CC_GC'GG
Cuts at: 2044 2044
Size: 5725
SalI G'TCGA_C
Cuts at: 1936 3067 1936
Size: 1131 4594
SbfI CC_TGCA'GG
Cuts at: 3077 3077
Size: 5725
ScaI AGT'ACT
Cuts at: 3973 5685 3973
Size: 1712 4013
SfcI C'TryA_G
Cuts at: 826 1045 2530 3073 4208 4889 5080 826
Size: 219 1485 543 1135 681 191 1471
Fragments arranged by size:
1485 1471 1135 681 543 219 191
SfoI GGC'GCC
Cuts at: 2125 2125
Size: 5725
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5725
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5725
SmaI CCC'GGG
Cuts at: 1872 3052 1872
Size: 1180 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3005 3829 4730 4992 820
Size: 197 926 1062 824 901 262 1553
Fragments arranged by size:
1553 1062 926 901 824 262 197
SnaBI TAC'GTA
Cuts at: 493 3516 493
Size: 3023 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5725
SphI G_CATG'C
Cuts at: 1132 2884 3083 1132
Size: 1752 199 3774
Fragments arranged by size:
3774 1752 199
SspI AAT'ATT
Cuts at: 5 52 2613 3649 5
Size: 47 2561 1036 2081
Fragments arranged by size:
2561 2081 1036 47
StuI AGG'CCT
Cuts at: 1517 2156 2217 2875 2907 1517
Size: 639 61 658 32 4335
Fragments arranged by size:
4335 658 639 61 32
StyI C'CwwG_G
Cuts at: 513 1065 2004 2287 2667 3164 513
Size: 552 939 283 380 497 3074
Fragments arranged by size:
3074 939 552 497 380 283
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2408 2513 3752 3769 3922 4107 4552
Size: 1255 828 105 1239 17 153 185 445
Cuts at: 4552 325
Size: 1498
Fragments arranged by size:
1498 1255 1239 828 445 185 153 105
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3971 5683 96
Size: 274 80 33 51 1443 1994 1712 138
Fragments arranged by size:
1994 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2786 2818 5685 5717 2786
Size: 32 2867 32 2794
Fragments arranged by size:
2867 2794 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5725
XbaI T'CTAG_A
Cuts at: 3061 3061
Size: 5725
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5725
XmaI C'CCGG_G
Cuts at: 1870 3050 1870
Size: 1180 4545
XmnI GAAnn'nnTTC
Cuts at: 2979 3854 2979
Size: 875 4850
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3533 276
Size: 53 83 186 936 1999 2468
Fragments arranged by size:
2468 1999 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI
BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI
EarI EciI Eco57MI EcoNI EcoO109I EcoRI EcoRV FspI
HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI
MluI MmeI MscI MslI MspA1I NarI NcoI NdeI
NheI NotI NspI PasI PciI PflMI PfoI PmeI
PpiI PpuMI PshAI PsiI PstI PvuI PvuII SacII
SalI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BfrBI
BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoICRI FalI
FseI FspAI NaeI NgoMIV NruI NsiI PacI PmlI
PspOMI PspXI PsrI RsrII SacI SanDI SapI SexAI
SfiI SrfI SwaI XcmI