Restriction map for
pFN21AB5369
(Circular) (Six-base) MAPSORT of: pFN21AB5369.seq Check: 5324 from: 1 to: 6277
pFN21AB5369 6277 bp
With 182 enzymes: SgfI *
April 15, 2010 09:25 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2048 3420 2048
Size: 1372 4905
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4087 278
Size: 53 83 186 936 2551 2468
Fragments arranged by size:
2551 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3620 1937
Size: 1683 4594
Acc65I G'GTAC_C
Cuts at: 3598 3598
Size: 6277
AclI AA'CG_TT
Cuts at: 1563 4404 4777 1563
Size: 2841 373 3063
Fragments arranged by size:
3063 2841 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2117 4340 5724 5992 1632
Size: 485 2223 1384 268 1917
Fragments arranged by size:
2223 1917 1384 485 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6080
AflIII A'CryG_T
Cuts at: 1203 2679 5176 6153 1203
Size: 1476 2497 977 1327
Fragments arranged by size:
2497 1476 1327 977
AgeI A'CCGG_T
Cuts at: 5108 5108
Size: 6277
AhdI GACnn_n'nnGTC
Cuts at: 5006 5006
Size: 6277
AleI CACnn'nnGTG
Cuts at: 1166 2150 2507 1166
Size: 984 357 4936
Fragments arranged by size:
4936 984 357
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6277
AlwNI CAG_nnn'CTG
Cuts at: 2405 2962 3049 4020 5592 2405
Size: 557 87 971 1572 3090
Fragments arranged by size:
3090 1572 971 557 87
ApaI G_GGCC'C
Cuts at: 3401 3401
Size: 6277
ApaLI G'TGCA_C
Cuts at: 4334 5490 4334
Size: 1156 5121
ApoI r'AATT_y
Cuts at: 1493 3586 3812 3964 6073 1493
Size: 2093 226 152 2109 1697
Fragments arranged by size:
2109 2093 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4831 160
Size: 872 3799 1606
Fragments arranged by size:
3799 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6277
AvaI C'yCGr_G
Cuts at: 1870 1943 3602 1870
Size: 73 1659 4545
Fragments arranged by size:
4545 1659 73
BamHI G'GATC_C
Cuts at: 3607 3974 3607
Size: 367 5910
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2036 2339 2381 3021 3598
Size: 325 217 627 249 303 42 640 577
Cuts at: 3598 5053 618
Size: 1455 1842
Fragments arranged by size:
1842 1455 640 627 577 325 303 249
217 42
BanII G_rGCy'C
Cuts at: 1391 3401 3596 1391
Size: 2010 195 4072
Fragments arranged by size:
4072 2010 195
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3117 3142 3379 928
Size: 971 1218 25 237 3826
Fragments arranged by size:
3826 1218 971 237 25
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4468 4502 1903
Size: 34 2531 34 3678
Fragments arranged by size:
3678 2531 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3158 4169 5379 3158
Size: 1011 1210 4056
Fragments arranged by size:
4056 1210 1011
BclI T'GATC_A
Cuts at: 1548 2655 1548
Size: 1107 5170
BfrBI ATG'CAT
Cuts at: 2632 2632
Size: 6277
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2546 3044 3509
Size: 107 122 71 631 605 874 498 465
Cuts at: 3509 4888 136
Size: 1379 1525
Fragments arranged by size:
1525 1379 874 631 605 498 465 122
107 71
BglII A'GATC_T
Cuts at: 6272 6272
Size: 6277
BlpI GC'TnA_GC
Cuts at: 3694 3694
Size: 6277
Bme1580I G_kGCm'C
Cuts at: 1790 2384 2738 3024 3348 3401 4338 5494 1790
Size: 594 354 286 324 53 937 1156 2573
Fragments arranged by size:
2573 1156 937 594 354 324 286 53
BmgBI CAC'GTC
Cuts at: 1318 2653 3148 3379 1318
Size: 1335 495 231 4216
Fragments arranged by size:
4216 1335 495 231
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2470 4008 4966
Size: 416 53 83 399 24 1053 1538 958
Cuts at: 4966 442
Size: 1753
Fragments arranged by size:
1753 1538 1053 958 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6277
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2464 3313 2464
Size: 849 5428
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2147 2285 4937 1239
Size: 908 138 2652 2579
Fragments arranged by size:
2652 2579 908 138
Bpu10I CC'TnA_GC
Cuts at: 3563 3563
Size: 6277
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2207 2614 4402 5267 5565 2207
Size: 407 1788 865 298 2919
Fragments arranged by size:
2919 1788 865 407 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2088 2249 2873 3353 4940 882
Size: 617 589 161 624 480 1587 2219
Fragments arranged by size:
2219 1587 624 617 589 480 161
BsaAI yAC'GTr
Cuts at: 493 1204 3451 4068 493
Size: 711 2247 617 2702
Fragments arranged by size:
2702 2247 711 617
BsaBI GATnn'nnATC
Cuts at: 6271 6271
Size: 6277
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3423 4084 4466 5101
Size: 53 83 186 936 1890 661 382 635
Cuts at: 5101 275
Size: 1451
Fragments arranged by size:
1890 1451 936 661 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 4710 5108 5382 5529 5940 4710
Size: 398 274 147 411 5047
Fragments arranged by size:
5047 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2783 2813 1178
Size: 30 165 30 1380 30 4642
Fragments arranged by size:
4642 1380 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2267 3094 1186
Size: 1081 827 4369
Fragments arranged by size:
4369 1081 827
BseYI C'CCAG_C
Cuts at: 2474 2525 5480 5976 2474
Size: 51 2955 496 2775
Fragments arranged by size:
2955 2775 496 51
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2441 3401 1163
Size: 573 705 960 4039
Fragments arranged by size:
4039 960 705 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3727 4488 5516 1540
Size: 275 186 1726 761 1028 2301
Fragments arranged by size:
2301 1726 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3499 4338 4423 5494 3499
Size: 839 85 1071 4282
Fragments arranged by size:
4282 1071 839 85
BsmI GAATG_Cn'
Cuts at: 3788 3881 3788
Size: 93 6184
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2384 2738 3024 3348 3401 3499 3596
Size: 399 594 354 286 324 53 98 97
Cuts at: 3596 4338 4423 5494 1391
Size: 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 594 399 354 324 286
98 97 85 53
BspEI T'CCGG_A
Cuts at: 5940 5940
Size: 6277
BspHI T'CATG_A
Cuts at: 4166 5896 4166
Size: 1730 4547
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2048 3420 3632 844
Size: 1204 1372 212 3489
Fragments arranged by size:
3489 1372 1204 212
BsrBI CCG'CTC
Cuts at: 1394 1823 4164 1394
Size: 429 2341 3507
Fragments arranged by size:
3507 2341 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3259 4772 4946 65
Size: 1183 2011 1513 174 1396
Fragments arranged by size:
2011 1513 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2336 4921 5108 1700
Size: 110 526 2585 187 2869
Fragments arranged by size:
2869 2585 526 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6277
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6277
BssSI C'ACGA_G
Cuts at: 2503 2737 3356 3428 4337 5349 2503
Size: 234 619 72 909 1012 3431
Fragments arranged by size:
3431 1012 909 619 234 72
BstAPI GCAn_nnn'nTGC
Cuts at: 794 3049 3415 794
Size: 2255 366 3656
Fragments arranged by size:
3656 2255 366
BstBI TT'CG_AA
Cuts at: 5081 5081
Size: 6277
BstEII G'GTnAC_C
Cuts at: 1184 2443 1184
Size: 1259 5018
BstXI CCAn_nnnn'nTGG
Cuts at: 2246 2246
Size: 6277
BstYI r'GATC_y
Cuts at: 1972 2958 3607 3974 4359 4376 5828 5914 5926
Size: 986 649 367 385 17 1452 86 12
Cuts at: 5926 6272 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 986 649 385 367 346 86
17 12
Bsu36I CC'TnA_GG
Cuts at: 2586 2586
Size: 6277
BtgI C'CryG_G
Cuts at: 513 1065 2385 3016 3123 3175 3300 3364 3402
Size: 552 1320 631 107 52 125 64 38
Cuts at: 3402 513
Size: 3388
Fragments arranged by size:
3388 1320 631 552 125 107 64 52
38
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2599 487
Size: 683 56 1373 4165
Fragments arranged by size:
4165 1373 683 56
BtsI GCAGTG_nn'
Cuts at: 3129 3234 3793 4587 4607 3129
Size: 105 559 794 20 4799
Fragments arranged by size:
4799 794 559 105 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6277
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6242
DraI TTT'AAA
Cuts at: 3581 3581
Size: 6277
DraIII CAC_nnn'GTG
Cuts at: 2851 2851
Size: 6277
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2923 3383 5284 809
Size: 507 1607 460 1901 1802
Fragments arranged by size:
1901 1802 1607 507 460
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2028 2668 2949
Size: 54 1425 50 275 18 198 640 281
Cuts at: 2949 3381 3487 3724 4613 6242 8
Size: 432 106 237 889 1629 43
Fragments arranged by size:
1629 1425 889 640 432 281 275 237
198 106 54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3724 1537
Size: 275 1912 4090
Fragments arranged by size:
4090 1912 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2768 2876 4207 1353
Size: 1415 108 1331 3423
Fragments arranged by size:
3423 1415 1331 108
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4864 5238 5384 387
Size: 1064 138 3275 374 146 1280
Fragments arranged by size:
3275 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2117 2147 2285 4340 4937 5724 5992
Size: 393 485 30 138 2055 597 787 268
Cuts at: 5992 1239
Size: 1524
Fragments arranged by size:
2055 1524 787 597 485 393 268 138
30
EcoNI CCTnn'n_nnAGG
Cuts at: 3053 3053
Size: 6277
EcoO109I rG'GnC_Cy
Cuts at: 2323 3046 3397 3398 3530 2323
Size: 723 351 1 132 5070
Fragments arranged by size:
5070 723 351 132 1
EcoRI G'AATT_C
Cuts at: 3586 3964 3586
Size: 378 5899
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2299 3247 2299
Size: 948 5329
FspI TGC'GCA
Cuts at: 4783 4783
Size: 6277
HaeII r_GCGC'y
Cuts at: 1678 2337 2532 5424 1678
Size: 659 195 2892 2531
Fragments arranged by size:
2892 2531 659 195
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2549 2581 3279 3311 4918
Size: 32 135 32 977 32 698 32 1607
Cuts at: 4918 4950 4992 5024 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1607 977 698 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2764 3621 3867 669
Size: 988 281 826 857 246 3079
Fragments arranged by size:
3079 988 857 826 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6277
HpaI GTT'AAC
Cuts at: 3867 3867
Size: 6277
KpnI G_GTAC'C
Cuts at: 3602 3602
Size: 6277
MfeI C'AATT_G
Cuts at: 3876 3876
Size: 6277
MluI A'CGCG_T
Cuts at: 6153 6153
Size: 6277
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5391 5575 1305
Size: 4086 184 2007
Fragments arranged by size:
4086 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2030 2951 10
Size: 54 1425 343 198 921 3336
Fragments arranged by size:
3336 1425 921 343 198 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2150 2507 2651 4235 4594 4753 518
Size: 648 984 357 144 1584 359 159 2042
Fragments arranged by size:
2042 1584 984 648 359 357 159 144
MspA1I CmG'CkG
Cuts at: 1606 3693 4370 5518 5763 1606
Size: 2087 677 1148 245 2120
Fragments arranged by size:
2120 2087 1148 677 245
NaeI GCC'GGC
Cuts at: 2338 2338
Size: 6277
NcoI C'CATG_G
Cuts at: 513 1065 3300 3402 513
Size: 552 2235 102 3388
Fragments arranged by size:
3388 2235 552 102
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6277
NgoMIV G'CCGG_C
Cuts at: 2336 2336
Size: 6277
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6277
NotI GC'GGCC_GC
Cuts at: 3724 3724
Size: 6277
NsiI A_TGCA'T
Cuts at: 2634 2634
Size: 6277
NspI r_CATG'y
Cuts at: 1132 1229 2683 2890 3635 5180 1132
Size: 97 1454 207 745 1545 2229
Fragments arranged by size:
2229 1545 1454 745 207 97
PasI CC'CwG_GG
Cuts at: 1348 2246 2431 1348
Size: 898 185 5194
Fragments arranged by size:
5194 898 185
PciI A'CATG_T
Cuts at: 2679 5176 2679
Size: 2497 3780
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2088 2851 3454 1108
Size: 980 763 603 3931
Fragments arranged by size:
3931 980 763 603
PmeI GTTT'AAAC
Cuts at: 3581 3581
Size: 6277
PmlI CAC'GTG
Cuts at: 3451 3451
Size: 6277
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4346 4378 5859 5891 4346
Size: 32 1481 32 4732
Fragments arranged by size:
4732 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2323 2323
Size: 6277
PshAI GACnn'nnGTC
Cuts at: 1613 3125 1613
Size: 1512 4765
PsiI TTA'TAA
Cuts at: 3847 3847
Size: 6277
PspOMI G'GGCC_C
Cuts at: 3397 3397
Size: 6277
PstI C_TGCA'G
Cuts at: 830 3529 3629 830
Size: 2699 100 3478
Fragments arranged by size:
3478 2699 100
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6277
SalI G'TCGA_C
Cuts at: 1936 3619 1936
Size: 1683 4594
SapI GCTCTTCn'nnn_
Cuts at: 2768 2768
Size: 6277
SbfI CC_TGCA'GG
Cuts at: 3629 3629
Size: 6277
ScaI AGT'ACT
Cuts at: 2025 2087 4525 6237 2025
Size: 62 2438 1712 2065
Fragments arranged by size:
2438 2065 1712 62
SfcI C'TryA_G
Cuts at: 826 1045 3525 3625 4760 5441 5632 826
Size: 219 2480 100 1135 681 191 1471
Fragments arranged by size:
2480 1471 1135 681 219 191 100
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6277
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6277
SmaI CCC'GGG
Cuts at: 1872 3604 1872
Size: 1732 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2222 2593 4381 5282 5544 820
Size: 197 926 279 371 1788 901 262 1553
Fragments arranged by size:
1788 1553 926 901 371 279 262 197
SnaBI TAC'GTA
Cuts at: 493 4068 493
Size: 3575 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6277
SphI G_CATG'C
Cuts at: 1132 3635 1132
Size: 2503 3774
SspI AAT'ATT
Cuts at: 5 52 4201 5
Size: 47 4149 2081
Fragments arranged by size:
4149 2081 47
StuI AGG'CCT
Cuts at: 1517 2591 1517
Size: 1074 5203
StyI C'CwwG_G
Cuts at: 513 1065 2466 3300 3393 3402 3716 513
Size: 552 1401 834 93 9 314 3074
Fragments arranged by size:
3074 1401 834 552 314 93 9
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2180 2444 4304 4321 4474 4659 5104
Size: 1255 600 264 1860 17 153 185 445
Cuts at: 5104 325
Size: 1498
Fragments arranged by size:
1860 1498 1255 600 445 264 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2023 2085 4523
Size: 274 80 33 51 1443 46 62 2438
Cuts at: 4523 6235 96
Size: 1712 138
Fragments arranged by size:
2438 1712 1443 274 138 80 62 51
46 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6237 6269 6237
Size: 32 6245
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6277
XbaI T'CTAG_A
Cuts at: 3613 3613
Size: 6277
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2243 2266 3400 2243
Size: 23 1134 5120
Fragments arranged by size:
5120 1134 23
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6277
XmaI C'CCGG_G
Cuts at: 1870 3602 1870
Size: 1732 4545
XmnI GAAnn'nnTTC
Cuts at: 2777 3230 4406 2777
Size: 453 1176 4648
Fragments arranged by size:
4648 1176 453
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4085 276
Size: 53 83 186 936 2551 2468
Fragments arranged by size:
2551 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI
BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI
BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI
DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoNI
EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII
HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I
NaeI NcoI NdeI NgoMIV NheI NotI NsiI NspI
PasI PciI PflMI PmeI PmlI PpiI PpuMI PshAI
PsiI PspOMI PstI PvuI SalI SapI SbfI ScaI
SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI AscI AvrII BaeI BbeI BbvCI BsiWI
BsmBI BstZ17I EcoICRI EcoRV FseI FspAI KasI NarI
NruI PacI PfoI PspXI PsrI PvuII RsrII SacI
SacII SanDI SexAI SfiI SfoI SrfI SwaI