Restriction map for
pFN21AB5356
(Circular) (Six-base) MAPSORT of: pFN21AB5356.seq Check: 1489 from: 1 to: 6049
pFN21AB5356 6049 bp
With 182 enzymes: SgfI *
October 20, 2009 10:35 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 2060 3859 278
Size: 53 83 186 936 524 1799 2468
Fragments arranged by size:
2468 1799 936 524 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3392 1937
Size: 1455 4594
Acc65I G'GTAC_C
Cuts at: 3370 3370
Size: 6049
AclI AA'CG_TT
Cuts at: 1563 4176 4549 1563
Size: 2613 373 3063
Fragments arranged by size:
3063 2613 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 4112 5496 5764 1632
Size: 2480 1384 268 1917
Fragments arranged by size:
2480 1917 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5852
AflIII A'CryG_T
Cuts at: 1203 4948 5925 1203
Size: 3745 977 1327
Fragments arranged by size:
3745 1327 977
AgeI A'CCGG_T
Cuts at: 4880 4880
Size: 6049
AhdI GACnn_n'nnGTC
Cuts at: 4778 4778
Size: 6049
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6049
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6049
AlwNI CAG_nnn'CTG
Cuts at: 3792 5364 3792
Size: 1572 4477
ApaI G_GGCC'C
Cuts at: 2117 2675 2117
Size: 558 5491
ApaLI G'TGCA_C
Cuts at: 4106 5262 4106
Size: 1156 4893
ApoI r'AATT_y
Cuts at: 1493 3358 3584 3736 5845 1493
Size: 1865 226 152 2109 1697
Fragments arranged by size:
2109 1865 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4603 160
Size: 872 3571 1606
Fragments arranged by size:
3571 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6049
AvaI C'yCGr_G
Cuts at: 1870 1943 2210 3095 3374 1870
Size: 73 267 885 279 4545
Fragments arranged by size:
4545 885 279 267 73
BamHI G'GATC_C
Cuts at: 3379 3746 3379
Size: 367 5682
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2774 2942 3370 4825 618
Size: 325 217 627 987 168 428 1455 1842
Fragments arranged by size:
1842 1455 987 627 428 325 217 168
BanII G_rGCy'C
Cuts at: 1391 2081 2117 2675 2865 3276 3368 1391
Size: 690 36 558 190 411 92 4072
Fragments arranged by size:
4072 690 558 411 190 92 36
BbeI G_GCGC'C
Cuts at: 2778 2778
Size: 6049
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2520 2985 928
Size: 971 621 465 3992
Fragments arranged by size:
3992 971 621 465
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 4240 4274 1903
Size: 34 50 34 2219 34 3678
Fragments arranged by size:
3678 2219 50 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3941 5151 3941
Size: 1210 4839
BclI T'GATC_A
Cuts at: 1548 2268 2439 2520 2706 1548
Size: 720 171 81 186 4891
Fragments arranged by size:
4891 720 186 171 81
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2103 4660 136
Size: 107 122 71 631 605 431 2557 1525
Fragments arranged by size:
2557 1525 631 605 431 122 107 71
BglII A'GATC_T
Cuts at: 6044 6044
Size: 6049
BlpI GC'TnA_GC
Cuts at: 3466 3466
Size: 6049
Bme1580I G_kGCm'C
Cuts at: 1790 2117 2675 2947 4110 5266 1790
Size: 327 558 272 1163 1156 2573
Fragments arranged by size:
2573 1163 1156 558 327 272
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6049
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2134 3780 4738
Size: 416 53 83 399 24 717 1646 958
Cuts at: 4738 442
Size: 1753
Fragments arranged by size:
1753 1646 958 717 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6049
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2249 2933 3080 4709 1239
Size: 1010 684 147 1629 2579
Fragments arranged by size:
2579 1629 1010 684 147
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2564 4174 5039 5337 2564
Size: 1610 865 298 3276
Fragments arranged by size:
3276 1610 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4712 882
Size: 617 3213 2219
Fragments arranged by size:
3213 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3840 493
Size: 711 2636 2702
Fragments arranged by size:
2702 2636 711
BsaBI GATnn'nnATC
Cuts at: 6043 6043
Size: 6049
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2057 2775 3856 4238
Size: 53 83 186 936 524 718 1081 382
Cuts at: 4238 4873 275
Size: 635 1451
Fragments arranged by size:
1451 1081 936 718 635 524 382 186
83 53
BsaWI w'CCGG_w
Cuts at: 4482 4880 5154 5301 5712 4482
Size: 398 274 147 411 4819
Fragments arranged by size:
4819 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5824
Fragments arranged by size:
5824 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2177 2189 2399 2923 3068 3083 1186
Size: 991 12 210 524 145 15 4152
Fragments arranged by size:
4152 991 524 210 145 15 12
BseYI C'CCAG_C
Cuts at: 5252 5748 5252
Size: 496 5553
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5476
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2073 2219 3311 3499 4260 5288
Size: 275 186 72 146 1092 188 761 1028
Cuts at: 5288 1540
Size: 2301
Fragments arranged by size:
2301 1092 1028 761 275 188 186 146
72
BsiHKAI G_wGCw'C
Cuts at: 2481 2580 4110 4195 5266 2481
Size: 99 1530 85 1071 3264
Fragments arranged by size:
3264 1530 1071 99 85
BsmI GAATG_Cn'
Cuts at: 2835 3560 3653 2835
Size: 725 93 5231
Fragments arranged by size:
5231 725 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2081 2117 2481 2580 2675 2865 2947
Size: 399 291 36 364 99 95 190 82
Cuts at: 2947 3276 3368 4110 4195 5266 1391
Size: 329 92 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 399 364 329 291 190
99 95 92 85 82 36
BspEI T'CCGG_A
Cuts at: 5712 5712
Size: 6049
BspHI T'CATG_A
Cuts at: 2436 2703 3938 5668 2436
Size: 267 1235 1730 2817
Fragments arranged by size:
2817 1730 1235 267
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3404 844
Size: 2560 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 2053 3936 1394
Size: 429 230 1883 3507
Fragments arranged by size:
3507 1883 429 230
BsrDI GCAATG_nn'
Cuts at: 65 1248 4544 4718 65
Size: 1183 3296 174 1396
Fragments arranged by size:
3296 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4693 4880 1700
Size: 110 2883 187 2869
Fragments arranged by size:
2883 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6049
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6049
BssSI C'ACGA_G
Cuts at: 2795 4109 5121 2795
Size: 1314 1012 3723
Fragments arranged by size:
3723 1314 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2655 794
Size: 1861 4188
BstBI TT'CG_AA
Cuts at: 4853 4853
Size: 6049
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6049
BstXI CCAn_nnnn'nTGG
Cuts at: 2470 2470
Size: 6049
BstYI r'GATC_y
Cuts at: 1972 3379 3746 4131 4148 5600 5686 5698 6044
Size: 1407 367 385 17 1452 86 12 346
Cuts at: 6044 1972
Size: 1977
Fragments arranged by size:
1977 1452 1407 385 367 346 86 17
12
BtgI C'CryG_G
Cuts at: 513 1065 2509 2598 2895 3141 3291 513
Size: 552 1444 89 297 246 150 3271
Fragments arranged by size:
3271 1444 552 297 246 150 89
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5310
Fragments arranged by size:
5310 683 56
BtsI GCAGTG_nn'
Cuts at: 2649 3565 4359 4379 2649
Size: 916 794 20 4319
Fragments arranged by size:
4319 916 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6049
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6014
DraI TTT'AAA
Cuts at: 3353 3353
Size: 6049
DraIII CAC_nnn'GTG
Cuts at: 2295 2295
Size: 6049
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2192 5056 809
Size: 507 876 2864 1802
Fragments arranged by size:
2864 1802 876 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2070 2216 2595
Size: 54 1425 50 275 18 240 146 379
Cuts at: 2595 3183 3308 3496 4385 6014 8
Size: 588 125 188 889 1629 43
Fragments arranged by size:
1629 1425 889 588 379 275 240 188
146 125 54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2070 2216 3308 3496 1537
Size: 275 258 146 1092 188 4090
Fragments arranged by size:
4090 1092 275 258 188 146
EarI CTCTTCn'nnn_
Cuts at: 1353 2229 2555 3060 3979 1353
Size: 876 326 505 919 3423
Fragments arranged by size:
3423 919 876 505 326
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4636 5010 5156 387
Size: 1064 138 3047 374 146 1280
Fragments arranged by size:
3047 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2249 2933 3080 4112 4709 5496 5764
Size: 393 617 684 147 1032 597 787 268
Cuts at: 5764 1239
Size: 1524
Fragments arranged by size:
1524 1032 787 684 617 597 393 268
147
EcoO109I rG'GnC_Cy
Cuts at: 2113 2179 2671 2672 2819 2113
Size: 66 492 1 147 5343
Fragments arranged by size:
5343 492 147 66 1
EcoRI G'AATT_C
Cuts at: 3358 3736 3358
Size: 378 5671
FspI TGC'GCA
Cuts at: 4555 4555
Size: 6049
HaeII r_GCGC'y
Cuts at: 1678 2407 2778 3242 5196 1678
Size: 729 371 464 1954 2531
Fragments arranged by size:
2531 1954 729 464 371
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2042 2074 2346 2375 2378
Size: 32 135 32 470 32 272 29 3
Cuts at: 2378 2407 2918 2950 4690 4722 4764 4796 1373
Size: 29 511 32 1740 32 42 32 2626
Fragments arranged by size:
2626 1740 511 470 272 135 42 32
32 32 32 32 32 29 29 3
HincII GTy'rAC
Cuts at: 669 1657 1938 3393 3639 669
Size: 988 281 1455 246 3079
Fragments arranged by size:
3079 1455 988 281 246
HindIII A'AGCT_T
Cuts at: 748 3086 748
Size: 2338 3711
HpaI GTT'AAC
Cuts at: 3639 3639
Size: 6049
KasI G'GCGC_C
Cuts at: 2774 2774
Size: 6049
KpnI G_GTAC'C
Cuts at: 3374 3374
Size: 6049
MfeI C'AATT_G
Cuts at: 3648 3648
Size: 6049
MluI A'CGCG_T
Cuts at: 5925 5925
Size: 6049
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5163 5347 1305
Size: 3858 184 2007
Fragments arranged by size:
3858 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2597 3185 10
Size: 54 1425 343 765 588 2874
Fragments arranged by size:
2874 1425 765 588 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2015 2303 2783 4007 4366 4525 518
Size: 648 849 288 480 1224 359 159 2042
Fragments arranged by size:
2042 1224 849 648 480 359 288 159
MspA1I CmG'CkG
Cuts at: 1606 2493 3150 3465 4142 5290 5535 1606
Size: 887 657 315 677 1148 245 2120
Fragments arranged by size:
2120 1148 887 677 657 315 245
NarI GG'CG_CC
Cuts at: 2775 2775
Size: 6049
NcoI C'CATG_G
Cuts at: 513 1065 2509 2598 2895 513
Size: 552 1444 89 297 3667
Fragments arranged by size:
3667 1444 552 297 89
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6049
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6049
NotI GC'GGCC_GC
Cuts at: 2216 3496 2216
Size: 1280 4769
NspI r_CATG'y
Cuts at: 1132 1229 2267 3407 4952 1132
Size: 97 1038 1140 1545 2229
Fragments arranged by size:
2229 1545 1140 1038 97
PasI CC'CwG_GG
Cuts at: 1348 3047 1348
Size: 1699 4350
PciI A'CATG_T
Cuts at: 4948 4948
Size: 6049
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2123 2901 1108
Size: 1015 778 4256
Fragments arranged by size:
4256 1015 778
PmeI GTTT'AAAC
Cuts at: 3353 3353
Size: 6049
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4118 4150 5631 5663 4118
Size: 32 1481 32 4504
Fragments arranged by size:
4504 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2179 2179
Size: 6049
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6049
PsiI TTA'TAA
Cuts at: 3619 3619
Size: 6049
PspOMI G'GGCC_C
Cuts at: 2113 2671 2113
Size: 558 5491
PstI C_TGCA'G
Cuts at: 830 2688 3401 830
Size: 1858 713 3478
Fragments arranged by size:
3478 1858 713
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6049
SalI G'TCGA_C
Cuts at: 1936 3391 1936
Size: 1455 4594
SapI GCTCTTCn'nnn_
Cuts at: 2229 2229
Size: 6049
SbfI CC_TGCA'GG
Cuts at: 3401 3401
Size: 6049
ScaI AGT'ACT
Cuts at: 4297 6009 4297
Size: 1712 4337
SexAI A'CCwGG_T
Cuts at: 3300 3300
Size: 6049
SfcI C'TryA_G
Cuts at: 826 1045 2197 2684 3112 3397 4532 5213 5404
Size: 219 1152 487 428 285 1135 681 191
Cuts at: 5404 826
Size: 1471
Fragments arranged by size:
1471 1152 1135 681 487 428 285 219
191
SfoI GGC'GCC
Cuts at: 2776 2776
Size: 6049
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6049
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6049
SmaI CCC'GGG
Cuts at: 1872 2212 3376 1872
Size: 340 1164 4545
Fragments arranged by size:
4545 1164 340
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2579 4153 5054 5316 820
Size: 197 926 636 1574 901 262 1553
Fragments arranged by size:
1574 1553 926 901 636 262 197
SnaBI TAC'GTA
Cuts at: 493 3840 493
Size: 3347 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6049
SphI G_CATG'C
Cuts at: 1132 3407 1132
Size: 2275 3774
SspI AAT'ATT
Cuts at: 5 52 3973 5
Size: 47 3921 2081
Fragments arranged by size:
3921 2081 47
StuI AGG'CCT
Cuts at: 1517 2226 2693 1517
Size: 709 467 4873
Fragments arranged by size:
4873 709 467
StyI C'CwwG_G
Cuts at: 513 1065 2509 2566 2598 2895 3488 513
Size: 552 1444 57 32 297 593 3074
Fragments arranged by size:
3074 1444 593 552 297 57 32
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 4076 4093 4246 4431 4876 325
Size: 1255 2496 17 153 185 445 1498
Fragments arranged by size:
2496 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4295 6007 96
Size: 274 80 33 51 1443 2318 1712 138
Fragments arranged by size:
2318 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2027 2059 6009 6041 2027
Size: 32 3950 32 2035
Fragments arranged by size:
3950 2035 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6049
XbaI T'CTAG_A
Cuts at: 3385 3385
Size: 6049
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2470 3298 2470
Size: 828 5221
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6049
XmaI C'CCGG_G
Cuts at: 1870 2210 3374 1870
Size: 340 1164 4545
Fragments arranged by size:
4545 1164 340
XmnI GAAnn'nnTTC
Cuts at: 4178 4178
Size: 6049
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 2058 3857 276
Size: 53 83 186 936 524 1799 2468
Fragments arranged by size:
2468 1799 936 524 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI
BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI
EarI EciI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I
HincII HindIII HpaI KasI KpnI MfeI MluI MmeI
MscI MslI MspA1I NarI NcoI NdeI NheI NotI
NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI
PsiI PspOMI PstI PvuI SalI SapI SbfI ScaI
SexAI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI AscI AvrII BaeI BbvCI BfrBI
BplI Bpu10I BsiWI BsmBI BstZ17I Bsu36I EcoICRI EcoNI
EcoRV FalI FseI FspAI NaeI NgoMIV NruI NsiI
PacI PfoI PmlI PspXI PsrI PvuII RsrII SacI
SacII SanDI SfiI SrfI SwaI