Restriction map for
pFN21AB5352
(Circular) (Six-base) MAPSORT of: pFN21AB5352.seq Check: 8173 from: 1 to: 6040
pFN21AB5352 6040 bp
With 182 enzymes: SgfI *
October 20, 2009 10:35 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3850 278
Size: 53 83 186 936 2314 2468
Fragments arranged by size:
2468 2314 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2157 2175 3383 1937
Size: 220 18 1208 4594
Fragments arranged by size:
4594 1208 220 18
Acc65I G'GTAC_C
Cuts at: 3361 3361
Size: 6040
AclI AA'CG_TT
Cuts at: 1563 4167 4540 1563
Size: 2604 373 3063
Fragments arranged by size:
3063 2604 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2240 2603 2612 4103 5487 5755 1632
Size: 608 363 9 1491 1384 268 1917
Fragments arranged by size:
1917 1491 1384 608 363 268 9
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5843
AflIII A'CryG_T
Cuts at: 1203 3066 4939 5916 1203
Size: 1863 1873 977 1327
Fragments arranged by size:
1873 1863 1327 977
AgeI A'CCGG_T
Cuts at: 4871 4871
Size: 6040
AhdI GACnn_n'nnGTC
Cuts at: 2751 3036 4769 2751
Size: 285 1733 4022
Fragments arranged by size:
4022 1733 285
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6040
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6040
AlwNI CAG_nnn'CTG
Cuts at: 3783 5355 3783
Size: 1572 4468
ApaLI G'TGCA_C
Cuts at: 4097 5253 4097
Size: 1156 4884
ApoI r'AATT_y
Cuts at: 1493 2075 2411 2885 3121 3349 3575 3727 5836
Size: 582 336 474 236 228 226 152 2109
Cuts at: 5836 1493
Size: 1697
Fragments arranged by size:
2109 1697 582 474 336 236 228 226
152
AseI AT'TA_AT
Cuts at: 160 1032 4594 160
Size: 872 3562 1606
Fragments arranged by size:
3562 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6040
AvaI C'yCGr_G
Cuts at: 1870 1943 3365 1870
Size: 73 1422 4545
Fragments arranged by size:
4545 1422 73
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 3037 3070 3037
Size: 33 6007
BamHI G'GATC_C
Cuts at: 3370 3737 3370
Size: 367 5673
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3361 4816 618
Size: 325 217 627 1574 1455 1842
Fragments arranged by size:
1842 1574 1455 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2929 3359 1391
Size: 1538 430 4072
Fragments arranged by size:
4072 1538 430
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2571 928
Size: 971 672 4397
Fragments arranged by size:
4397 971 672
BbvCI CC'TCA_GC
Cuts at: 2939 2939
Size: 6040
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4231 4265 1903
Size: 34 2294 34 3678
Fragments arranged by size:
3678 2294 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3932 5142 3932
Size: 1210 4830
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6040
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4651 136
Size: 107 122 71 631 605 2979 1525
Fragments arranged by size:
2979 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2830 6035 2830
Size: 3205 2835
BlpI GC'TnA_GC
Cuts at: 3457 3457
Size: 6040
Bme1580I G_kGCm'C
Cuts at: 1790 4101 5257 1790
Size: 2311 1156 2573
Fragments arranged by size:
2573 2311 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6040
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3322 3771 4729
Size: 416 53 83 399 24 1905 449 958
Cuts at: 4729 442
Size: 1753
Fragments arranged by size:
1905 1753 958 449 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6040
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 3078 4700 1239
Size: 1839 1622 2579
Fragments arranged by size:
2579 1839 1622
Bpu10I CC'TnA_GC
Cuts at: 2939 2939
Size: 6040
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3011 3306 4165 5030 5328 3011
Size: 295 859 865 298 3723
Fragments arranged by size:
3723 865 859 298 295
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2579 2661 4703 882
Size: 617 1080 82 2042 2219
Fragments arranged by size:
2219 2042 1080 617 82
BsaAI yAC'GTr
Cuts at: 493 1204 3831 493
Size: 711 2627 2702
Fragments arranged by size:
2702 2627 711
BsaBI GATnn'nnATC
Cuts at: 2052 6034 2052
Size: 3982 2058
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3847 4229 4864 275
Size: 53 83 186 936 2314 382 635 1451
Fragments arranged by size:
2314 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4473 4871 5145 5292 5703 4473
Size: 398 274 147 411 4810
Fragments arranged by size:
4810 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5815
Fragments arranged by size:
5815 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 6040
BseYI C'CCAG_C
Cuts at: 5243 5739 5243
Size: 496 5544
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5467
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3490 4251 5279 1540
Size: 275 186 1489 761 1028 2301
Fragments arranged by size:
2301 1489 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 4101 4186 5257 4101
Size: 85 1071 4884
Fragments arranged by size:
4884 1071 85
BsmI GAATG_Cn'
Cuts at: 3551 3644 3551
Size: 93 5947
BsmBI CGTCTCn'nnnn_
Cuts at: 3020 3020
Size: 6040
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2929 3359 4101 4186 5257 1391
Size: 399 1139 430 742 85 1071 2174
Fragments arranged by size:
2174 1139 1071 742 430 399 85
BspEI T'CCGG_A
Cuts at: 5703 5703
Size: 6040
BspHI T'CATG_A
Cuts at: 3929 5659 3929
Size: 1730 4310
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2809 3285 3395 844
Size: 1965 476 110 3489
Fragments arranged by size:
3489 1965 476 110
BsrBI CCG'CTC
Cuts at: 1394 1823 3927 1394
Size: 429 2104 3507
Fragments arranged by size:
3507 2104 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4535 4709 65
Size: 1183 3287 174 1396
Fragments arranged by size:
3287 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4684 4871 1700
Size: 110 2874 187 2869
Fragments arranged by size:
2874 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6040
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6040
BssSI C'ACGA_G
Cuts at: 4100 5112 4100
Size: 1012 5028
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6040
BstBI TT'CG_AA
Cuts at: 2806 4844 2806
Size: 2038 4002
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6040
BstYI r'GATC_y
Cuts at: 1972 2293 2830 3370 3737 4122 4139 5591 5677
Size: 321 537 540 367 385 17 1452 86
Cuts at: 5677 5689 6035 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 540 537 385 367 346 321
86 17 12
BstZ17I GTA'TAC
Cuts at: 2176 2176
Size: 6040
BtgI C'CryG_G
Cuts at: 513 1065 2004 2559 513
Size: 552 939 555 3994
Fragments arranged by size:
3994 939 555 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2017 487
Size: 683 56 791 4510
Fragments arranged by size:
4510 791 683 56
BtsI GCAGTG_nn'
Cuts at: 3556 4350 4370 3556
Size: 794 20 5226
Fragments arranged by size:
5226 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6040
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2218 2253 529
Size: 35 1654 35 4316
Fragments arranged by size:
4316 1654 35 35
DraI TTT'AAA
Cuts at: 3344 3344
Size: 6040
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5047 809
Size: 507 3731 1802
Fragments arranged by size:
3731 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3258 3487 4376
Size: 54 1425 50 275 18 1428 229 889
Cuts at: 4376 6005 8
Size: 1629 43
Fragments arranged by size:
1629 1428 1425 889 275 229 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3487 1537
Size: 275 1675 4090
Fragments arranged by size:
4090 1675 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2631 2714 3023 3970 1353
Size: 1278 83 309 947 3423
Fragments arranged by size:
3423 1278 947 309 83
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2024 2372 4627 5001 5147 387
Size: 1064 138 435 348 2255 374 146 1280
Fragments arranged by size:
2255 1280 1064 435 374 348 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2240 2603 2612 3078 4103 4700 5487
Size: 393 608 363 9 466 1025 597 787
Cuts at: 5487 5755 1239
Size: 268 1524
Fragments arranged by size:
1524 1025 787 608 597 466 393 363
268 9
EcoRI G'AATT_C
Cuts at: 3349 3727 3349
Size: 378 5662
FspI TGC'GCA
Cuts at: 4546 4546
Size: 6040
HaeII r_GCGC'y
Cuts at: 1678 5187 1678
Size: 3509 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2737 2769 3022 3054 4681
Size: 32 135 32 1165 32 253 32 1627
Cuts at: 4681 4713 4755 4787 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1627 1165 253 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2322 2908 3384 3630 669
Size: 988 281 384 586 476 246 3079
Fragments arranged by size:
3079 988 586 476 384 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6040
HpaI GTT'AAC
Cuts at: 2908 3630 2908
Size: 722 5318
KpnI G_GTAC'C
Cuts at: 3365 3365
Size: 6040
MfeI C'AATT_G
Cuts at: 2274 3639 2274
Size: 1365 4675
MluI A'CGCG_T
Cuts at: 5916 5916
Size: 6040
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5154 5338 1305
Size: 3849 184 2007
Fragments arranged by size:
3849 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 3260 10
Size: 54 1425 343 1428 2790
Fragments arranged by size:
2790 1428 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3065 3998 4357 4516 518
Size: 648 1899 933 359 159 2042
Fragments arranged by size:
2042 1899 933 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 3456 4133 5281 5526 1606
Size: 1850 677 1148 245 2120
Fragments arranged by size:
2120 1850 1148 677 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 2559 513
Size: 552 939 555 3994
Fragments arranged by size:
3994 939 555 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6040
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6040
NotI GC'GGCC_GC
Cuts at: 3487 3487
Size: 6040
NruI TCG'CGA
Cuts at: 2691 2691
Size: 6040
NspI r_CATG'y
Cuts at: 1132 1229 3070 3398 4943 1132
Size: 97 1841 328 1545 2229
Fragments arranged by size:
2229 1841 1545 328 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6040
PciI A'CATG_T
Cuts at: 3066 4939 3066
Size: 1873 4167
PflMI CCAn_nnn'nTGG
Cuts at: 1108 3256 1108
Size: 2148 3892
PmeI GTTT'AAAC
Cuts at: 3344 3344
Size: 6040
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2460 2492 4109 4141 5622 5654 2460
Size: 32 1617 32 1481 32 2846
Fragments arranged by size:
2846 1617 1481 32 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6040
PsiI TTA'TAA
Cuts at: 3610 3610
Size: 6040
PstI C_TGCA'G
Cuts at: 830 3392 830
Size: 2562 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6040
SalI G'TCGA_C
Cuts at: 1936 3382 1936
Size: 1446 4594
SbfI CC_TGCA'GG
Cuts at: 3392 3392
Size: 6040
ScaI AGT'ACT
Cuts at: 4288 6000 4288
Size: 1712 4328
SfcI C'TryA_G
Cuts at: 826 1045 2398 2778 3388 4523 5204 5395 826
Size: 219 1353 380 610 1135 681 191 1471
Fragments arranged by size:
1471 1353 1135 681 610 380 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6040
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6040
SmaI CCC'GGG
Cuts at: 1872 3367 1872
Size: 1495 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2990 3321 4144 5045 5307 820
Size: 197 926 1047 331 823 901 262 1553
Fragments arranged by size:
1553 1047 926 901 823 331 262 197
SnaBI TAC'GTA
Cuts at: 493 3831 493
Size: 3338 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6040
SphI G_CATG'C
Cuts at: 1132 3398 1132
Size: 2266 3774
SspI AAT'ATT
Cuts at: 5 52 2384 2698 3268 3964 5
Size: 47 2332 314 570 696 2081
Fragments arranged by size:
2332 2081 696 570 314 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6040
StyI C'CwwG_G
Cuts at: 513 1065 2004 2559 3479 513
Size: 552 939 555 920 3074
Fragments arranged by size:
3074 939 920 555 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 4067 4084 4237 4422 4867 325
Size: 1255 2487 17 153 185 445 1498
Fragments arranged by size:
2487 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4286 5998 96
Size: 274 80 33 51 1443 2309 1712 138
Fragments arranged by size:
2309 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6000 6032 6000
Size: 32 6008
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6040
XbaI T'CTAG_A
Cuts at: 3376 3376
Size: 6040
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2423 2423
Size: 6040
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6040
XmaI C'CCGG_G
Cuts at: 1870 3365 1870
Size: 1495 4545
XmnI GAAnn'nnTTC
Cuts at: 2969 4169 2969
Size: 1200 4840
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3848 276
Size: 53 83 186 936 2314 2468
Fragments arranged by size:
2468 2314 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BaeI BamHI BanI BanII BbsI BbvCI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BstZ17I
BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI
EagI EarI EciI Eco57MI EcoRI FspI HaeII Hin4I
HincII HindIII HpaI KpnI MfeI MluI MmeI MscI
MslI MspA1I NcoI NdeI NheI NotI NruI NspI
PasI PciI PflMI PmeI PpiI PshAI PsiI PstI
PvuI SalI SbfI ScaI SfcI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BbeI BfrBI
BplI BsiWI BstXI Bsu36I DraIII EcoICRI EcoNI EcoO109I
EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV
NsiI PacI PfoI PmlI PpuMI PspOMI PspXI PsrI
PvuII RsrII SacI SacII SanDI SapI SexAI SfiI
SfoI SrfI SwaI