Restriction map for
pFN21AB5344
(Circular) (Six-base) MAPSORT of: pFN21AB5344.seq Check: 2178 from: 1 to: 5401
pFN21AB5344 5401 bp
With 182 enzymes: SgfI *
January 16, 2009 15:25 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3211 278
Size: 53 83 186 936 1675 2468
Fragments arranged by size:
2468 1675 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2744 1937
Size: 807 4594
Acc65I G'GTAC_C
Cuts at: 2722 2722
Size: 5401
AclI AA'CG_TT
Cuts at: 1563 3528 3901 1563
Size: 1965 373 3063
Fragments arranged by size:
3063 1965 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3464 4848 5116 1632
Size: 1832 1384 268 1917
Fragments arranged by size:
1917 1832 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5204
AflIII A'CryG_T
Cuts at: 1203 4300 5277 1203
Size: 3097 977 1327
Fragments arranged by size:
3097 1327 977
AgeI A'CCGG_T
Cuts at: 4232 4232
Size: 5401
AhdI GACnn_n'nnGTC
Cuts at: 4130 4130
Size: 5401
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5401
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2478 2499 1184
Size: 1294 21 4086
Fragments arranged by size:
4086 1294 21
AlwNI CAG_nnn'CTG
Cuts at: 3144 4716 3144
Size: 1572 3829
ApaI G_GGCC'C
Cuts at: 2023 2023
Size: 5401
ApaLI G'TGCA_C
Cuts at: 3458 4614 3458
Size: 1156 4245
ApoI r'AATT_y
Cuts at: 1493 2710 2936 3088 5197 1493
Size: 1217 226 152 2109 1697
Fragments arranged by size:
2109 1697 1217 226 152
AseI AT'TA_AT
Cuts at: 160 1032 3955 160
Size: 872 2923 1606
Fragments arranged by size:
2923 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5401
AvaI C'yCGr_G
Cuts at: 1870 1943 2726 1870
Size: 73 783 4545
Fragments arranged by size:
4545 783 73
BamHI G'GATC_C
Cuts at: 2731 3098 2731
Size: 367 5034
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2437 2722 4177 618
Size: 325 217 627 650 285 1455 1842
Fragments arranged by size:
1842 1455 650 627 325 285 217
BanII G_rGCy'C
Cuts at: 1391 2023 2600 2720 1391
Size: 632 577 120 4072
Fragments arranged by size:
4072 632 577 120
BbeI G_GCGC'C
Cuts at: 2441 2441
Size: 5401
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2087 928
Size: 971 188 4242
Fragments arranged by size:
4242 971 188
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2243 2277 3592 3626 1903
Size: 34 306 34 1315 34 3678
Fragments arranged by size:
3678 1315 306 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2197 3293 4503 2197
Size: 1096 1210 3095
Fragments arranged by size:
3095 1210 1096
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5401
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4012 136
Size: 107 122 71 631 605 2340 1525
Fragments arranged by size:
2340 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5396 5396
Size: 5401
BlpI GC'TnA_GC
Cuts at: 2506 2818 2506
Size: 312 5089
Bme1580I G_kGCm'C
Cuts at: 1790 2023 2136 3462 4618 1790
Size: 233 113 1326 1156 2573
Fragments arranged by size:
2573 1326 1156 233 113
BmgBI CAC'GTC
Cuts at: 1318 2068 1318
Size: 750 4651
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2631 3132 4090
Size: 416 53 83 399 24 1214 501 958
Cuts at: 4090 442
Size: 1753
Fragments arranged by size:
1753 1214 958 501 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5401
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 4061 1239
Size: 2822 2579
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2300 3526 4391 4689 2300
Size: 1226 865 298 3012
Fragments arranged by size:
3012 1226 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2117 4064 882
Size: 617 618 1947 2219
Fragments arranged by size:
2219 1947 618 617
BsaAI yAC'GTr
Cuts at: 493 1204 3192 493
Size: 711 1988 2702
Fragments arranged by size:
2702 1988 711
BsaBI GATnn'nnATC
Cuts at: 2115 5395 2115
Size: 3280 2121
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2438 3208 3590 4225
Size: 53 83 186 936 905 770 382 635
Cuts at: 4225 275
Size: 1451
Fragments arranged by size:
1451 936 905 770 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 3834 4232 4506 4653 5064 3834
Size: 398 274 147 411 4171
Fragments arranged by size:
4171 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2610 2640 1178
Size: 30 165 30 1207 30 3939
Fragments arranged by size:
3939 1207 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 5401
BseYI C'CCAG_C
Cuts at: 2025 2351 2479 4604 5100 2025
Size: 326 128 2125 496 2326
Fragments arranged by size:
2326 2125 496 326 128
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2090 2505 1163
Size: 573 354 415 4059
Fragments arranged by size:
4059 573 415 354
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2851 3612 4640 1540
Size: 275 186 850 761 1028 2301
Fragments arranged by size:
2301 1028 850 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3462 3547 4618 3462
Size: 85 1071 4245
Fragments arranged by size:
4245 1071 85
BsmI GAATG_Cn'
Cuts at: 2912 3005 2912
Size: 93 5308
BsmBI CGTCTCn'nnnn_
Cuts at: 2008 2008
Size: 5401
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2023 2136 2600 2720 3462 3547 4618
Size: 399 233 113 464 120 742 85 1071
Cuts at: 4618 1391
Size: 2174
Fragments arranged by size:
2174 1071 742 464 399 233 120 113
85
BspEI T'CCGG_A
Cuts at: 5064 5064
Size: 5401
BspHI T'CATG_A
Cuts at: 3290 5020 3290
Size: 1730 3671
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2397 2756 844
Size: 1553 359 3489
Fragments arranged by size:
3489 1553 359
BsrBI CCG'CTC
Cuts at: 1394 1823 3288 1394
Size: 429 1465 3507
Fragments arranged by size:
3507 1465 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3896 4070 65
Size: 1183 2648 174 1396
Fragments arranged by size:
2648 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4045 4232 1700
Size: 110 2235 187 2869
Fragments arranged by size:
2869 2235 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5401
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5401
BssSI C'ACGA_G
Cuts at: 3461 4473 3461
Size: 1012 4389
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5401
BstBI TT'CG_AA
Cuts at: 2145 4205 2145
Size: 2060 3341
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5401
BstYI r'GATC_y
Cuts at: 1972 2213 2731 3098 3483 3500 4952 5038 5050
Size: 241 518 367 385 17 1452 86 12
Cuts at: 5050 5396 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 518 385 367 346 241 86
17 12
Bsu36I CC'TnA_GG
Cuts at: 2209 2209
Size: 5401
BtgI C'CryG_G
Cuts at: 513 1065 513
Size: 552 4849
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2102 487
Size: 683 56 876 3786
Fragments arranged by size:
3786 876 683 56
BtsI GCAGTG_nn'
Cuts at: 2078 2582 2917 3711 3731 2078
Size: 504 335 794 20 3748
Fragments arranged by size:
3748 794 504 335 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5401
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5366
DraI TTT'AAA
Cuts at: 2705 2705
Size: 5401
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4408 809
Size: 507 3092 1802
Fragments arranged by size:
3092 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2037 2848 3737
Size: 54 1425 50 275 18 207 811 889
Cuts at: 3737 5366 8
Size: 1629 43
Fragments arranged by size:
1629 1425 889 811 275 207 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2848 1537
Size: 275 1036 4090
Fragments arranged by size:
4090 1036 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2588 2662 3331 1353
Size: 1235 74 669 3423
Fragments arranged by size:
3423 1235 669 74
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3988 4362 4508 387
Size: 1064 138 2399 374 146 1280
Fragments arranged by size:
2399 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 3464 4061 4848 5116 1239
Size: 393 1832 597 787 268 1524
Fragments arranged by size:
1832 1524 787 597 393 268
EcoO109I rG'GnC_Cy
Cuts at: 2020 2020
Size: 5401
EcoRI G'AATT_C
Cuts at: 2710 3088 2710
Size: 378 5023
FspI TGC'GCA
Cuts at: 3907 3907
Size: 5401
HaeII r_GCGC'y
Cuts at: 1678 2441 4548 1678
Size: 763 2107 2531
Fragments arranged by size:
2531 2107 763
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2034 2066 4042 4074 4116
Size: 32 135 32 462 32 1976 32 42
Cuts at: 4116 4148 1373
Size: 32 2626
Fragments arranged by size:
2626 1976 462 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2745 2991 669
Size: 988 281 807 246 3079
Fragments arranged by size:
3079 988 807 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5401
HpaI GTT'AAC
Cuts at: 2991 2991
Size: 5401
KasI G'GCGC_C
Cuts at: 2437 2437
Size: 5401
KpnI G_GTAC'C
Cuts at: 2726 2726
Size: 5401
MfeI C'AATT_G
Cuts at: 3000 3000
Size: 5401
MluI A'CGCG_T
Cuts at: 5277 5277
Size: 5401
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4515 4699 1305
Size: 3210 184 2007
Fragments arranged by size:
3210 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2039 10
Size: 54 1425 343 207 3372
Fragments arranged by size:
3372 1425 343 207 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3359 3718 3877 518
Size: 648 2193 359 159 2042
Fragments arranged by size:
2193 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2479 2817 3494 4642 4887 1606
Size: 873 338 677 1148 245 2120
Fragments arranged by size:
2120 1148 873 677 338 245
NarI GG'CG_CC
Cuts at: 2438 2438
Size: 5401
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 4849
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5401
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5401
NotI GC'GGCC_GC
Cuts at: 2848 2848
Size: 5401
NspI r_CATG'y
Cuts at: 1132 1229 2759 4304 1132
Size: 97 1530 1545 2229
Fragments arranged by size:
2229 1545 1530 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5401
PciI A'CATG_T
Cuts at: 4300 4300
Size: 5401
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5401
PmeI GTTT'AAAC
Cuts at: 2705 2705
Size: 5401
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3470 3502 4983 5015 3470
Size: 32 1481 32 3856
Fragments arranged by size:
3856 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5401
PsiI TTA'TAA
Cuts at: 2971 2971
Size: 5401
PspOMI G'GGCC_C
Cuts at: 2019 2019
Size: 5401
PstI C_TGCA'G
Cuts at: 830 2753 830
Size: 1923 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5401
PvuII CAG'CTG
Cuts at: 2479 2479
Size: 5401
SalI G'TCGA_C
Cuts at: 1936 2743 1936
Size: 807 4594
SapI GCTCTTCn'nnn_
Cuts at: 2588 2588
Size: 5401
SbfI CC_TGCA'GG
Cuts at: 2753 2753
Size: 5401
ScaI AGT'ACT
Cuts at: 2407 3649 5361 2407
Size: 1242 1712 2447
Fragments arranged by size:
2447 1712 1242
SfcI C'TryA_G
Cuts at: 826 1045 2248 2749 3884 4565 4756 826
Size: 219 1203 501 1135 681 191 1471
Fragments arranged by size:
1471 1203 1135 681 501 219 191
SfoI GGC'GCC
Cuts at: 2439 2439
Size: 5401
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5401
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5401
SmaI CCC'GGG
Cuts at: 1872 2728 1872
Size: 856 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2315 3505 4406 4668 820
Size: 197 926 372 1190 901 262 1553
Fragments arranged by size:
1553 1190 926 901 372 262 197
SnaBI TAC'GTA
Cuts at: 493 3192 493
Size: 2699 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5401
SphI G_CATG'C
Cuts at: 1132 2759 1132
Size: 1627 3774
SspI AAT'ATT
Cuts at: 5 52 3325 5
Size: 47 3273 2081
Fragments arranged by size:
3273 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5401
StyI C'CwwG_G
Cuts at: 513 1065 2840 513
Size: 552 1775 3074
Fragments arranged by size:
3074 1775 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2471 3428 3445 3598 3783 4228 325
Size: 1255 891 957 17 153 185 445 1498
Fragments arranged by size:
1498 1255 957 891 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2405 3647 5359
Size: 274 80 33 51 1443 428 1242 1712
Cuts at: 5359 96
Size: 138
Fragments arranged by size:
1712 1443 1242 428 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2057 2089 5361 5393 2057
Size: 32 3272 32 2065
Fragments arranged by size:
3272 2065 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5401
XbaI T'CTAG_A
Cuts at: 2737 2737
Size: 5401
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5401
XmaI C'CCGG_G
Cuts at: 1870 2726 1870
Size: 856 4545
XmnI GAAnn'nnTTC
Cuts at: 3530 3530
Size: 5401
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3209 276
Size: 53 83 186 936 1675 2468
Fragments arranged by size:
2468 1675 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI
BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI
EarI EciI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I
HincII HindIII HpaI KasI KpnI MfeI MluI MmeI
MscI MslI MspA1I NarI NcoI NdeI NheI NotI
NspI PasI PciI PflMI PmeI PpiI PshAI PsiI
PspOMI PstI PvuI PvuII SalI SapI SbfI ScaI
SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI AscI AvrII BaeI BbvCI BfrBI
BplI Bpu10I BsiWI BstXI BstZ17I DraIII EcoICRI EcoNI
EcoRV FalI FseI FspAI NaeI NgoMIV NruI NsiI
PacI PfoI PmlI PpuMI PspXI PsrI RsrII SacI
SacII SanDI SexAI SfiI SrfI SwaI XcmI